Characterization and cloning of WUSCHEL enhancers from Arabidopsis thaliana:
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 100 Bl. Ill., graph. Darst. 30 cm |
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MARC
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Record in the Search Index
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TABLE OF CONTENTS
TABLE OF CONTENTS 4
ABSTRACT 10
AIMS OF THIS STUDY 12
INTRODUCTION 13
/. GENERAL INTRODUCTION TO PLANT MERISTEMS: THEY MAKE THE WHOLE PLANT
BODY 13
2. A CLOSER LOOK AT THE SAM: FUNCTION AND DOMAINS O F THE SAM 14
3. THE STEM CELL CONCEPT IN PLANTS: A NICHE CONCEPT WITH DYNAMIC CELL
EQUILIBRIUM 16
4. WUS IS REQUIRED F O R STEM CELL MAINTENANCE 16
5. FACTORS INVOLVED IN REGULATING STEM CELL NUMBER TOGETHER WITH WUS 17
6. TWO FACTORS INVOLVED IN WUS-STEM CELL REGULATORY PATHWAY WERE
IDENTIFIED 17
RESULTS 19
1. CLONING AND ANALYSIS O F WEN9 19
1.1. WHAT IS THE ENHANCED PHENOTYPE OF WEN9 WUS-61 19
1.2. WEN9 ENCODES OBERON3 25
1.3. OBE3-2 ENHANCES FLORAL MERISTEM BUT NOT SHOOT MERISTEM DEFECTS IN
WUS-7 30
1.4. OBE3-2 DID NOT ENHANCE THE SHOOT MERISTEM DEFECTS OF WUS-1 32
1.5. OBE3 FUNCTIONS REDUNDANTLY WITH OBE4 32
1.6. EXPRESSION LEVELS OF WUS AND CLV3 ARE REDUCED IN OBE3-2, BUT NOT IN
OBE4-2 36
1.7. ECTOPIC WUS EXPRESSION INDIRECTLY INDUCES THE EXPRESSION OF BOTH
OBE3 AND OBE4....
37
1.8. OVEREXPRESSION OF OBE3 ALLEVIATES SHOOT MERISTEM BUT NOT FLORAL
MERISTEM DEFECTS OF
WUS-1 40
1.9. EMBRYONIC POBE3 IS EXPRESSED UBIQUITOUSLY FROM THE 16-CELL STAGE 44
2. CHARACTERIZATION O F WEN 1 46
2.1. PENETRANCE OF WEITL ENHANCEMENT OF WUS-6 IS LOWER THAN EXPECTED 46
4
HTTP://D-NB.INFO/1025553284
IMAGE 2
2.2. PLANTS OF WENL WUS-6/+ HAVE DEPRESSED FEMALE FERTILITY 47
2.3. A PORTION OF WEITL WUS-6/+ PROGENY HAVE EMBRYONIC DEFECTS 47
2.4. A PORTION OF WENL WUS-6/+ SEED WERE ABNORMAL 50
2.5. THE ABNORMAL SEEDS O F WENL, WENL WUS-6/+ AND WENL/+ WUS-6/+ PLANTS
DID NOT
GERMINATE 52
DISCUSSION 54
1. GENERAL KNOWLEDGE O F OBES 54
2. OBE3/OBE4 ARE REDUNDANTLY INVOLVED IN MERISTEM REGULATION 54
3. OBE3 FUNCTIONS IN THE SAME PATHWAY AS WUS IN SAM ESTABLISHMENT. 55
4. OBE3 AND OBE4 PLAY DIFFERENT ROLES IN INFLORESCENCE/FLORAL MERISTEM
MAINTENANCE 56
5. HOW DO WUS AND OBE INTERACT? 56
6. HOMOZYGOSITY O F THE WENL LINE, AND EMBRYO PHENOTYPE CLASSIFICATION O
F DOUBLE MUTANTS.... 59
7. THE LOW RATIO O F ENHANCED WUS SEEDLINGS FROM WENL WUS-6/+ PARENTS
COULD BE EXPLAINED BY
THE EMBRYO DEFECT/SEED DEFECT 59
8. PUTATIVE NOVEL FUNCTION O F WUS IN EARLY EMBRYOGENESIS 60
9. IS REDUCED FEMALE FERTILITY O F WENL WUS-6/+ PLANTS GAMETOPHYTIC OR
SPOROPHYTIC? 60
10. THE PHENOTYPIC SEVERITY O F WENL MIGHT BE AFFECTED BY STRESS 61
11. WHAT GOES WRONG IN THE WENL EMBRYO? 62
12. HOW DOES WEN1 INTERACT WITH WUS? 62
13. MAPPING O F WEN 1 63
MATERIALS AND METHODS 64
MATERIAL: 64
MUTANT LINES 64
BUFFERS AND MEDIA 64
BACTERIAL STRAINS 64
5
IMAGE 3
FINAL CONCENTRATION O F ANTIBIOTICS USED IN GROWTH MEDIA 65
PLASMIDS, OLIGONUCLEOTIDES AND DNA SEQUENCING 65
CHEMICALS, ENZYMES AND KITS 66
METHODS: 67
PLANT WORK 67
SURFACE STERILIZATION O F SEEDS 67
ISOLATION OF PLANT DNA 68
PREPARATION OF TAQ DNA POLYMERASE FOR PCR-BASED GENOTYPING 69
MAPPING, GENETIC ANALYSIS AND PCR GENOTYPING OF WEN9 72
PCR-BASED GENOTYPING 73
TRANSFORMATION OF ARABIDOPSIS PLANTS 74
SELECTION OF TRANSGENIC PLANTS 75
CLONING OF DNA 76
PREPARATION OF POBE3:GOBE3 CONSTRUCTS OF AND SELECTION OF TRANSFORMANTS
76
QUANTITATIVE RT-PCR ANALYSIS 77
REFERENCES 78
ABBREVIATIONS 84
PHENOTYPIC NOMENCLATURE 85
PUBLICATIONS 86
APPENDIXES 87
ACKNOWLEDGEMENT 99
6
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| author | Lin, Ta-Fang |
| author_GND | (DE-588)1025456114 |
| author_facet | Lin, Ta-Fang |
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| author_sort | Lin, Ta-Fang |
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| building | Verbundindex |
| bvnumber | BV040522071 |
| ctrlnum | (OCoLC)820380285 (DE-599)DNB1025553284 |
| dewey-full | 575.482364 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 575 - Specific parts of & systems in plants |
| dewey-raw | 575.482364 |
| dewey-search | 575.482364 |
| dewey-sort | 3575.482364 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| spelling | Lin, Ta-Fang Verfasser (DE-588)1025456114 aut Characterization and cloning of WUSCHEL enhancers from Arabidopsis thaliana vorgelegt von Ta-Fang Lin 2012 100 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Freiburg (Breisgau), Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025368349&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lin, Ta-Fang Characterization and cloning of WUSCHEL enhancers from Arabidopsis thaliana |
| subject_GND | (DE-588)4113937-9 |
| title | Characterization and cloning of WUSCHEL enhancers from Arabidopsis thaliana |
| title_auth | Characterization and cloning of WUSCHEL enhancers from Arabidopsis thaliana |
| title_exact_search | Characterization and cloning of WUSCHEL enhancers from Arabidopsis thaliana |
| title_full | Characterization and cloning of WUSCHEL enhancers from Arabidopsis thaliana vorgelegt von Ta-Fang Lin |
| title_fullStr | Characterization and cloning of WUSCHEL enhancers from Arabidopsis thaliana vorgelegt von Ta-Fang Lin |
| title_full_unstemmed | Characterization and cloning of WUSCHEL enhancers from Arabidopsis thaliana vorgelegt von Ta-Fang Lin |
| title_short | Characterization and cloning of WUSCHEL enhancers from Arabidopsis thaliana |
| title_sort | characterization and cloning of wuschel enhancers from arabidopsis thaliana |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025368349&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT lintafang characterizationandcloningofwuschelenhancersfromarabidopsisthaliana |