Evaluation of equine bronchoalveolar lavage fluid (BALF) and tracheal aspirate sample (TAs) in normal and recurrent airway obstruction (RAO) horses:
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Berlin
Mensch und Buch-Verl.
2010 [erschienen] 2011
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | X, 134 S. Ill., graph. Darst. 21 cm |
| ISBN: | 9783866649187 |
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MARC
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| 100 | 1 | |a Shawaf, Turke |e Verfasser |0 (DE-588)1011358905 |4 aut | |
| 245 | 1 | 0 | |a Evaluation of equine bronchoalveolar lavage fluid (BALF) and tracheal aspirate sample (TAs) in normal and recurrent airway obstruction (RAO) horses |c submitted by Turke Shawaf |
| 264 | 1 | |a Berlin |b Mensch und Buch-Verl. |c 2010 [erschienen] 2011 | |
| 300 | |a X, 134 S. |b Ill., graph. Darst. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Berlin, Freie Univ., Diss., 2011 | ||
| 650 | 7 | |a bronchoalveolar lavage |2 cabt | |
| 650 | 7 | |a chronic obstructive pulmonary disease |2 cabt | |
| 650 | 7 | |a cd8+ lymphocytes |2 cabt | |
| 650 | 7 | |a cd4+ lymphocytes |2 cabt | |
| 650 | 7 | |a flow cytometry |2 cabt | |
| 650 | 7 | |a horses |2 cabt | |
| 650 | 4 | |a Pferd | |
| 650 | 0 | 7 | |a Pferd |0 (DE-588)4045503-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Broncho-alveoläre Lavage |0 (DE-588)4214640-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Obstruktive Ventilationsstörung |0 (DE-588)4213128-5 |2 gnd |9 rswk-swf |
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Record in the Search Index
| _version_ | 1804149602876653568 |
|---|---|
| adam_text | IMAGE 1
CONTENT I
CONTENT
LIST OF TABLES IV
LIST OF FIGURES V
LIST OF ABBREVIATIONS. XI
1 INTRODUCTION 1
2 REVIEW OF THE LITERATURE 3
2.1 TERMINOLOGY 3
2.2 ETIOLOGY AND PATIIOGENESIS 5
2.2.1 BACTERIA 8
2.2.1.1 ENDOTOXINS 8
2.2.2 FUNGI 9
2.2.2.1 GLUCAN 10
2.2.2.2 ASPERGILLUS FUMIGATUS 11
2.2.3 NOXIOUS GASES 11
2.3 PATHOPHYSIOLOGY OF RAO 12
2.3.1 AIRWAY OBSTRUCTION (BRONCHOSPASM) 12
2.3.2 AIRWAY INFLAMMATION 14
2.3.2.1 NEUTROPHILS 14
2.3.2.2 LYMPHOCYTES 15
2.3.2.3 MAST CELLS 17
2.3.2.4 EOSINOPHILS 18
2.3.2.5 MACROPHAGES 18
2.3.2.6 EPITHELIAL CELLS 19
2.3.3 MUCUS ACCUMULATION 19
2.3.4 AIRWAY REMODELING 20
2.4 CLINICAL SIGNS 20
2.5 ASSESSMENT OF LUNG FUNCTION 22
2.5.1 CLINICAL SCORES 22
2.5.2 ARTERIAL BLOOD GAS ANALYIS 23
2.5.2.1 ARTERIAL CARBON DIOXIDE TENSION (PACO2 24
2.5.2.2 ARTERIAL OXYGEN TENSION (PAO 2) 24
2.5.2.3 ALVEOLAR-ARTERIAL OXYGEN TENSION DIFFERENCE (P(A-A)O:) (A - ADO
2) 26 2.5.3 ENDOSCOPY OF THE EQUINE LOWER AIRWAY TRACT 26
2.5.3.1 ENDOSCOPIC SCORING SYSTEMS 27
2.5.3.2 INTERPRETATION OF TRACHEAL ASPIRATES 28
2.5.3.2.1 EPITHELIAL CELLS 29
2.5.3.2.2 MACROPHAGES 30
2.5.3.2.3 NEUTROPHILS 30
2.5.3.2.4 LYMPHOCYTES 32
2.5.3.2.5 EOSINOPHILS 32
2.5.3.2.6 MAST CELLS 33
2.5.3.2.7 MICROORGANISMS, DEBRIS, AND ARTIFACTS 33
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1010548530
DIGITALISIERT DURCH
IMAGE 2
CONTENT II
2.5.4 BRONCHOALVEOLAR LAVAGE (BAL) 33
2.5.4.1 TOTAL CELL COUNT 35
2.5.4.2 DIFFERENTIAL CELL COUNTS 35
2.5.4.2.1 MACROPHAGES 36
2.5.4.2.2 LYMPHOCYTES 37
2.5.4.2.3 NEUTROPHILS 38
2.5.4.2.4 MAST CELLS 38
2.5.4.2.5 EOSINOPHILS 39
2.5.4.2.6 EPITHELIAL CELLS 39
2.5.4.2.7 GLOBULE LEUKOCYTES 41
2.5.4.3 PHENOTYPIC ANALYSIS OF BALF LYMPHOCYTES BY FLOW CYTOMETRY 42
2.6 MANAGEMENT 44
2.7 THERAPY 46
3 MATERIALS AND METHODS 51
3.1 HORSES 51
3.2 CLINICAL EXAMINATION 52
3.3 ENDOSCOPY (CLINICAL SCORE) 54
3.4 SAMPLES 56
3.4.1 PERIPHERAL BLOOD 56
3.4.2 ARTERIAL BLOOD 57
3.4.2.1 PUNCTURE TECHNIQUE 58
3.4.2.2 SAMPLE HANDLING 58
3.4.3 TRACHEAL ASPIRATE SAMPLES 60
3.4.3.1 COLLECTION OF TRACHEAL ASPIRATE SAMPLES 60
3.4.3.2 LABORATORY ANALYSIS OF TRACHEAL ASPIRATE SAMPLES 73
3.4.4 BRONCHOALVEOLAR LAVAGE FLUID (BALF) 64
3.4.4.1 SAMPLE COLLECTION 64
3.4.4.2 TOTAL CELL COUNT 66
3.4.4.2.1 COUNTING TECHNIQUE 67
3.4.4.2.2 CALCULATION 68
3.4.4.3 DIFFERENTIAL CELL COUNTS 68
3.4.5 ANALYSIS OF T CELL SUBPOPULATIONS IN BALF BY FLOW CYTOMETRY 69
3.5 STATISTICAL ANALYSIS 70
4 RESULTS 71
4.1 CLINICAL EXAMINATION (CLINIC SCORE) 71
4 .2 ENDOSCOPY (SCORE) 72
4.3 BLOOD GAS ANALYSIS 73
4.3.1 TOTAL CELL COUNT 75
4.3.2 DIFFERENTIAL CELL COUNTS 76
IMAGE 3
CONTENT III
4.4 TRACHEAL ASPIRATE SAMPLES 78
4.5 COMPARISON IN DIFFERENTIAL CELL COUNTS BETWEEN BALF AND TAS 80
4.5.1 NEUTROPHILS 80
4.5.2 MACROPHAGE 81
4.5.3 LYMPHOCYTE 82
4.5.4 MAST CELLS 83
4.5.5 EOSINOPHILS 84
4.6 CD4 + AND CD8+ IN BLOOD AND BALF 85
5 DISCUSSION 90
5.1 GENERAL ASPECTS 90
5.2 DISCUSSION OF THE RESULTS 91
5.2.1 RESULTS OF CLINICAL EXAMINATION 91
5.2.2 TRACHEAL ENDOSCOPY 92
5.2.3 BLOOD GAS ANALYSIS 93
5.2.4 TOTAL CELL COUNTS IN BALF 93
5.2.5 DIFFERENTIAL CELL COUNTS BETWEEN BALF AND TAS 94
5.2.5.1 NEUTROPHILS 95
5.2.5.2 MACROPHAGE 96
5.2.5.3 LYMPHOCYTE 97
5.2.5.4 MAST CELLS 98
5.2.5.5 EOSINOPHILS 98
5.2.6 RESULTS OF CD4 + AND CD8+ IN BLOOD AND BALF 99
5.3 GENERAL CONCLUSIONS 100
6 SUMMARY 102
7 ZUSAMMENFASSUNG 104
8 REFERENCE 107
9 ACKNOWLEDGMENTS 133
10 SELBSTAENDIGKEITSERKLAERUNG 134
IMAGE 4
LIST OF TABLES IV
LIST OF TABLES
TABLE 1: RELATIVE PROPORTION (%) OF CELLS IN TA OBTAINED FROM HORSES
WITHOUT CLINICAL SIGNS OF RESPIRATORY DISEASE 29
TABLE 2: REFERENCE VALUES FOR BAL CYTOLOGY OF CLINICALLY NORMAL HORSES
36
TABLE 3: BRONCHOALVEOLAR LAVAGE CYTOLOGY OF HORSES WITH SIGNS OF
RESPIRATORY DISEASE 41
TABLE 4: THE PERCENTAGE OF T-LYMPHOCYTE SUBPOPULATION IN BALF IN NORMAL
AND RAO- AFFECTED HORSES 44
TABLE 5: PHYSICAL EXAMINATION AND CLINICAL SCORING 53
TABLE 6: ENDOSCOPIC EVALUATION WITH SCORE POINT 55
|
| any_adam_object | 1 |
| author | Shawaf, Turke |
| author_GND | (DE-588)1011358905 |
| author_facet | Shawaf, Turke |
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| author_sort | Shawaf, Turke |
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| building | Verbundindex |
| bvnumber | BV040521118 |
| classification_rvk | XX 4104 |
| ctrlnum | (OCoLC)830931806 (DE-599)DNB1010548530 |
| dewey-full | 636.1089624075 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 636 - Animal husbandry |
| dewey-raw | 636.1089624075 |
| dewey-search | 636.1089624075 |
| dewey-sort | 3636.1089624075 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau Medizin |
| format | Thesis Book |
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| indexdate | 2024-07-10T00:25:39Z |
| institution | BVB |
| isbn | 9783866649187 |
| language | English |
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| physical | X, 134 S. Ill., graph. Darst. 21 cm |
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| spelling | Shawaf, Turke Verfasser (DE-588)1011358905 aut Evaluation of equine bronchoalveolar lavage fluid (BALF) and tracheal aspirate sample (TAs) in normal and recurrent airway obstruction (RAO) horses submitted by Turke Shawaf Berlin Mensch und Buch-Verl. 2010 [erschienen] 2011 X, 134 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Berlin, Freie Univ., Diss., 2011 bronchoalveolar lavage cabt chronic obstructive pulmonary disease cabt cd8+ lymphocytes cabt cd4+ lymphocytes cabt flow cytometry cabt horses cabt Pferd Pferd (DE-588)4045503-8 gnd rswk-swf Broncho-alveoläre Lavage (DE-588)4214640-9 gnd rswk-swf Obstruktive Ventilationsstörung (DE-588)4213128-5 gnd rswk-swf Diagnostik (DE-588)4113303-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Pferd (DE-588)4045503-8 s Obstruktive Ventilationsstörung (DE-588)4213128-5 s Broncho-alveoläre Lavage (DE-588)4214640-9 s Diagnostik (DE-588)4113303-1 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025367409&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Shawaf, Turke Evaluation of equine bronchoalveolar lavage fluid (BALF) and tracheal aspirate sample (TAs) in normal and recurrent airway obstruction (RAO) horses bronchoalveolar lavage cabt chronic obstructive pulmonary disease cabt cd8+ lymphocytes cabt cd4+ lymphocytes cabt flow cytometry cabt horses cabt Pferd Pferd (DE-588)4045503-8 gnd Broncho-alveoläre Lavage (DE-588)4214640-9 gnd Obstruktive Ventilationsstörung (DE-588)4213128-5 gnd Diagnostik (DE-588)4113303-1 gnd |
| subject_GND | (DE-588)4045503-8 (DE-588)4214640-9 (DE-588)4213128-5 (DE-588)4113303-1 (DE-588)4113937-9 |
| title | Evaluation of equine bronchoalveolar lavage fluid (BALF) and tracheal aspirate sample (TAs) in normal and recurrent airway obstruction (RAO) horses |
| title_auth | Evaluation of equine bronchoalveolar lavage fluid (BALF) and tracheal aspirate sample (TAs) in normal and recurrent airway obstruction (RAO) horses |
| title_exact_search | Evaluation of equine bronchoalveolar lavage fluid (BALF) and tracheal aspirate sample (TAs) in normal and recurrent airway obstruction (RAO) horses |
| title_full | Evaluation of equine bronchoalveolar lavage fluid (BALF) and tracheal aspirate sample (TAs) in normal and recurrent airway obstruction (RAO) horses submitted by Turke Shawaf |
| title_fullStr | Evaluation of equine bronchoalveolar lavage fluid (BALF) and tracheal aspirate sample (TAs) in normal and recurrent airway obstruction (RAO) horses submitted by Turke Shawaf |
| title_full_unstemmed | Evaluation of equine bronchoalveolar lavage fluid (BALF) and tracheal aspirate sample (TAs) in normal and recurrent airway obstruction (RAO) horses submitted by Turke Shawaf |
| title_short | Evaluation of equine bronchoalveolar lavage fluid (BALF) and tracheal aspirate sample (TAs) in normal and recurrent airway obstruction (RAO) horses |
| title_sort | evaluation of equine bronchoalveolar lavage fluid balf and tracheal aspirate sample tas in normal and recurrent airway obstruction rao horses |
| topic | bronchoalveolar lavage cabt chronic obstructive pulmonary disease cabt cd8+ lymphocytes cabt cd4+ lymphocytes cabt flow cytometry cabt horses cabt Pferd Pferd (DE-588)4045503-8 gnd Broncho-alveoläre Lavage (DE-588)4214640-9 gnd Obstruktive Ventilationsstörung (DE-588)4213128-5 gnd Diagnostik (DE-588)4113303-1 gnd |
| topic_facet | bronchoalveolar lavage chronic obstructive pulmonary disease cd8+ lymphocytes cd4+ lymphocytes flow cytometry horses Pferd Broncho-alveoläre Lavage Obstruktive Ventilationsstörung Diagnostik Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025367409&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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