Functional analysis of the target of the Rapamycin (TOR) signaling pathway in Arabidopsis thaliana:
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XI, 133 S. Ill., graph. Darst. |
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Record in the Search Index
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| adam_text | IMAGE 1
TABLE O F CONTENTS
SELBSTANDIGKEITSERKLARUNG I
TABLE O F CONTENTS II
ALLGEMEINVERSTANDLICHE ZUSAMMENFASSUNG V
ABSTRACT VI
AIMS O F THE PHD THESIS VII
LIST O F FIGURES VIII
LIST O F TABLES IX
ABBREVIATIONS X
1. INTRODUCTION 1
1.1 TARGET O F RAPAMYCIN (TOR) 1
1.1.1 TARGET O F RAPAMYCIN 1
1.1.2 TWO TOR COMPLEXES-TORCL AND TORC2 3
1.2 UPSTREAM REGULATION O F THE TOR SIGNALING PATHWAY 6
1.2.1 INPUTS O F TORC1 6
1.2.2 INPUTS O F TORC2 7
1.3 DOWNSTREAM REGULATION O F THE TOR SIGNALING PATHWAY 8
1.3.1 TRANSLATION 8
1.3.2 AUTOPHAGY 9
1.3.3 OTHER FUNCTIONS O F TORC1 10
1.3.4 DOWNSTREAM REGULATION O F TORC2 11
1.4 THE TOR SIGNALING PATHWAY IN OTHER ORGANISMS 11
1.5 THE TOR SIGNALING PATHWAY IN ARABIDOPSIS THALIANA 12
1.6 DOWNSTREAM TARGETS O F TOR IN ARABIDOPSIS THALIANA 15
1.7 SYSTEMS APPROACH TO UNDERSTAND THE FUNCTION O F TOR 16
2. MATERIALS AND METHODS 19
2.1 CHEMICALS 19
2.2 SOFTWARE 19
2.3 FLUORESCEIN DIACETIC ACID (FDA) STAINING 19
2.4 ARABIDOPSIS THALIANA CELL SUSPENSION CULTURES 20
2.5 SUCROSE STARVATION-INDUCED SYNCHRONIZATION 20
2.6 CELL NUMBER DETERMINATION 20
2.7 DNA EXTRACTION 20
2.8 ISOLATION O F T-DNA INSERTION MUTANTS 21
2.9 ESTRADIOL INDUCIBLE ARTIFICIAL MICRO RNAI MEDIATED GENE SILENCING 21
2.10 PLANT GROWTH CONDITIONS 21
2.10.1 PLANT GROWTH CONDITIONS IN STERILE CULTURE 21
2.10.2 PLANT GROWTH CONDITIONS IN PHYTOTRONS AND GREENHOUSE 22
II
HTTP://D-NB.INFO/1036908178
IMAGE 2
2.11 EXPERIMENTAL DESIGN 22
2.12 METABOLITE EXTRACTION, MEASUREMENTS AND DATA ANALYSIS 23
2.12.1 METABOLITE EXTRACTION 23
2.12.2 PRIMARY METABOLITES ANALYSIS 23
2.12.3 LIPIDS AND SECONDARY METABOLITES ANALYSIS 24
2.12.4 DATA NORMALIZATION 25
2.12 RNA PREPARATION, REAL-TIME RT-PCR AND DATA ANALYSIS 26
2.13 AFFYMETRIX AND TRANSCRIPT DATA ANALYSIS 27
2.14 STARCH MEASUREMENT 28
2.15 WESTERN BLOT ANALYSIS O F ALTERNATIVE OXIDASE (AOX) 28
3. RESULTS 30
3.1 INSENSITIVITY O F ARABIDOPSIS SUSPENSION CELL CULTURE TO RAPAMYCIN
30
3.2 ISOLATION O F T-DNA LINES FOR GENES INVOLVED IN THE TOR SIGNALING IN
ARABIDOPSIS 32
3.3 DOWN-REGULATE TORC1 COMPONENTS BY ESTRADIOL INDUCIBLE ARTIFICIAL
MICRORNAI 34
3.3.1 CONDITIONAL DOWN-REGULATION O F TOR OR RAPTORL AFFECTS PLANT
GROWTH IN ARABIDOPSIS 34
3.3.2 EXPERIMENTAL SETUP FOR THE METABOLIC AND TRANSCRIPTIONAL PROFILING
O F TORC1 DEREGULATION
35
3.3.3 INHIBITION O F ATTOR OR ATRAPTORL LEVEL INDUCES COORDINATED
CHANGES IN THE TRANSCRIPT PROFILE
O F ARABIDOPSIS THALIANA 36
3.3.4 COMPREHENSIVE METABOLITE ANALYSIS O F AMIR_FOR AND AMIR _RAPTORL
PLANTS REVEALS
COORDINATED CHANGES IN THE TCA CYCLE AND AMINO ACIDS METABOLISM 39
3.3.5 SUPPRESSION O F ATTOR OR ATRAPTORL REALLOCATES CARBON STORAGE AND
ENERGY METABOLITES.... 43
3.3.6 INHIBITION O F ATTOR OR ATRAPTORL LEVEL LEADS TO CHANGES IN LIPID
METABOLISM 43
3.3.7 REPRESSION O F ATTOR OR ATRAPTORL LEADS TO CHANGE IN
PHENYLPROPANOID AND GLUCOSINOLATE
METABOLITES 46
3.4 DOWN-REGULATION O F TORC1 DOWNSTREAM TARGET -S6KS BY ESTRADIOL
INDUCIBLE ARTIFICIAL
MICRO RN AS 48
3.4.1 INDUCED AMIR_S6&./ AND AMIR _S6K2 LINES AFFECT PLANT GROWTH IN
ARABIDOPSIS 48
3.4.2 INHIBITION O F ATS6KS LEADS TO A DIFFERENT METABOLIC REPROGRAMMING
COMPARED TO TORC1
DEPLETION 50
3.4.3 ATS6KS REPROGRAM LIPID METABOLISM IN ARABIDOPSIS 53
3.4.4 ATS6KS SLIGHTLY AFFECTS PHENYLPROPANOID AND GLUCOSINOLATE
BIOSYNTHETIC PATHWAYS 55
4. DISCUSSION 56
4.1 RAPAMYCIN SENSITIVITY O F ARABIDOPSIS THALIANA 56
4.2 REPRESSING ATTOR OR ATRAPTORL IMPAIRS PLANT DEVELOPMENT 57
4.3 ROLE O F TORC1 IN TRANSCRIPTIONAL CONTROL IN ARABIDOPSIS 58
4.4 ROLE O F THE TOR SIGNALING IN PRIMARY METABOLIC CONTROL IN
ARABIDOPSIS 60
4.4.1 TORC1 AND AMINO ACID METABOLISM IN ARABIDOPSIS 61
4.4.2 TORC1 AND THE TCA CYCLE IN ARABIDOPSIS 65
III
IMAGE 3
4.4.3 TORC1 AND CARBON METABOLISM IN ARABIDOPSIS 67
4.4.4 TORC1 AND NITROGEN METABOLISM 68
4.5 ROLE O F THE TOR SIGNALING IN SECONDARY METABOLIC CONTROL IN
ARABIDOPSIS 70
4.6 ROLE O F THE TOR SIGNALING IN LIPID CONTROL IN ARABIDOPSIS 71
4.7 SUMMARY O F THE FUNCTIONS O F THE TORC1 COMPONENTS IN THE METABOLIC
REGULATION IN ARABIDOPSIS
THALIANA 73
4.8 DIFFERENT ROLES O F ATS6KS FROM THE TORC1 IN ARABIDOPSIS THALIANA 78
5. SUMMARY 81
6. REFERENCES 83
ACKNOWLEDGEMENTS 109
CURRICULUM VITAE 110
ACADEMIC QUALIFICATIONS 110
PUBLICATIONS 110
CONFERENCES I L L
SUPPLEMENTARY MATERIAL 112
IV
|
| any_adam_object | 1 |
| author | Li, Yan |
| author_GND | (DE-588)1026994713 |
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| author_sort | Li, Yan |
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| bvnumber | BV040491750 |
| ctrlnum | (OCoLC)828784366 (DE-599)GBV726277649 |
| dewey-full | 610 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 610 - Medicine and health |
| dewey-raw | 610 |
| dewey-search | 610 |
| dewey-sort | 3610 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Thesis Book |
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| language | English |
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| spelling | Li, Yan Verfasser (DE-588)1026994713 aut Functional analysis of the target of the Rapamycin (TOR) signaling pathway in Arabidopsis thaliana von Yan Li 2012 XI, 133 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Potsdam, Univ., Diss., 2012 Transkriptomanalyse (DE-588)4775956-2 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf Sirolimus (DE-588)4458353-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Sirolimus (DE-588)4458353-9 s Signaltransduktion (DE-588)4318717-1 s Transkriptomanalyse (DE-588)4775956-2 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025338724&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Li, Yan Functional analysis of the target of the Rapamycin (TOR) signaling pathway in Arabidopsis thaliana Transkriptomanalyse (DE-588)4775956-2 gnd Signaltransduktion (DE-588)4318717-1 gnd Ackerschmalwand (DE-588)4141299-0 gnd Sirolimus (DE-588)4458353-9 gnd |
| subject_GND | (DE-588)4775956-2 (DE-588)4318717-1 (DE-588)4141299-0 (DE-588)4458353-9 (DE-588)4113937-9 |
| title | Functional analysis of the target of the Rapamycin (TOR) signaling pathway in Arabidopsis thaliana |
| title_auth | Functional analysis of the target of the Rapamycin (TOR) signaling pathway in Arabidopsis thaliana |
| title_exact_search | Functional analysis of the target of the Rapamycin (TOR) signaling pathway in Arabidopsis thaliana |
| title_full | Functional analysis of the target of the Rapamycin (TOR) signaling pathway in Arabidopsis thaliana von Yan Li |
| title_fullStr | Functional analysis of the target of the Rapamycin (TOR) signaling pathway in Arabidopsis thaliana von Yan Li |
| title_full_unstemmed | Functional analysis of the target of the Rapamycin (TOR) signaling pathway in Arabidopsis thaliana von Yan Li |
| title_short | Functional analysis of the target of the Rapamycin (TOR) signaling pathway in Arabidopsis thaliana |
| title_sort | functional analysis of the target of the rapamycin tor signaling pathway in arabidopsis thaliana |
| topic | Transkriptomanalyse (DE-588)4775956-2 gnd Signaltransduktion (DE-588)4318717-1 gnd Ackerschmalwand (DE-588)4141299-0 gnd Sirolimus (DE-588)4458353-9 gnd |
| topic_facet | Transkriptomanalyse Signaltransduktion Ackerschmalwand Sirolimus Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025338724&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT liyan functionalanalysisofthetargetoftherapamycintorsignalingpathwayinarabidopsisthaliana |