Single domain antibodies: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
2012
|
| Schriftenreihe: | Methods in molecular biology
911 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XV, 580 S. Ill., graph. Darst. 27 cm |
Internformat
MARC
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| 245 | 1 | 0 | |a Single domain antibodies |b methods and protocols |c ed. by Dirk Saerens and Serge Muyldermans |
| 264 | 1 | |a New York |b Humana Press |c 2012 | |
| 300 | |a XV, 580 S. |b Ill., graph. Darst. |c 27 cm | ||
| 336 | |b txt |2 rdacontent | ||
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| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 911 | |
| 490 | 0 | |a Springer protocols | |
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| 650 | 4 | |a Monoclonal antibodies / Laboratory manuals | |
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Datensatz im Suchindex
| _version_ | 1804149549372014592 |
|---|---|
| adam_text | Titel: Single domain antibodies
Autor: Saerens, Dirk
Jahr: 2012
Contents
Preface.......................................................... v
Contributors...................................................... xj
Part I Overview of Single Domain Antibodies
1 From Whole Monoclonal Antibodies to Single Domain Antibodies:
Think Small.................................................. 3
Jean-Luc Teillaud
2 Introduction to Heavy Chain Antibodies and Derived Nanobodies......... 15
Cécile Vincke and Serge Muyldermans
3 Overview and Discovery of IgNARs and Generation of VNARs............ 27
Stewart D. Nuttall
Part II Single Domain Antibody Library Construction
4 Creation of the Large and Highly Functional Synthetic Repertoire
of Human VH and Vk Domain Antibodies........................... 39
Olga Ignatovich, Laurent Jespers, Ian M. Tomlinson,
and Ruud M.T. de Wildt
5 Preparation of a Naive Library of Camelid Single Domain Antibodies....... 65
Aurelien Olichon andArio de Marco
Part III Selection of Single Domain Antibodies
6 Selection by Phage Display of Single Domain Antibodies Specific
to Antigens in Their Native Conformation........................... 81
Peter Verheesen and Toon Laeremans
7 Semiautomated Panning of Naive Camelidae Libraries and Selection
of Single-Domain Antibodies Against Peptide Antigens.................. 105
Jyothi Kumaran, C. Roger MacKenzie, and Mehdi Arbabi-Ghahroudi
8 Pichia Surface Display: A Tool for Screening Single Domain Antibodies..... 125
KristofDe Schutter and Nico Callewaert
9 Bacterial Two Hybrid: A Versatile One-Step
Intracellular Selection Method.................................... 135
Mireille Pellis, Serge Muyldermans, and Cécile Vincke
10 Intracellular Antibody Capture (IAC) Methods
for Single Domain Antibodies..................................... 151
Tomoyuki Tanaka and Terence H. Rabbitts
VII
viii Contents
11 Selection of Functional Single Domain Antibody Fragments for Interfering
with Protein-Protein Interactions Inside Cells: A One Plasmid
Mammalian Two-Hybrid System................................... 175
Tomoyuki Tanaka and Terence H. Rabbitts
12 Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization . 183
Armin Sepp and Andrew Griffiths
13 Selection ofVHHs Under Application Conditions..................... 199
Edward Dolk, Theo Verrips, and Hans de Haard
14 Isolation and Characterization of Clostridium difficile Toxin-Specific
Single-Domain Antibodies....................................... 211
Greg Hussack, Mehdi Arbabi-Ghahroudi, C. Roger MacKenzie,
andjamshid Tanha
15 Selection of VHH Antibody Fragments That Recognize Different
Aß Depositions Using Complex Immune Libraries..................... 241
Rinse Klooster, Kim S. Rutgers, and Silvere M. van der Maarel
Part IV Expression of Single Domain Antibodies and Derivatives
16 Expression of Single-Domain Antibodies in Bacterial Systems............. 257
Toya Nath Baral and Mehdi Arbabi-Ghahroudi
17 Expression of VHHs in Saccharomyces cerevisiae........................ 277
Andrea Gorlani, Hans de Haard, and Theo Verrips
18 Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells....... 287
Vishal Agrawal, Igor Slivac, Sylvie Perret, Louis Bisson,
Gilles St-Laurent, Yanal Murad, Jianbing Zhang, and Yves Durocher
19 Production of Camel-Like Antibodies in Plants........................ 305
Sylvie De Buck, Vikratn Virdi, Thomas De Meyer, Kirsten De Wilde,
Robin Piron, Jonah Nolf, Els Van Lerberge, Annelies De Paepe,
and Ann Depicker
Part V Improvement and Applications of Single Domain Antibodies
20 Selecting and Purifying Autonomous Human
Variable Heavy (VH) Domains.................................... 327
Raffi Tonikian and Sachdev S. Sidhu
21 Solubility and Stability Engineering of Human VH Domains............. 355
Dae Toung Kim, Wen Ding, andjamshid Tanha
22 Improvement of Proteolytic Stability Through In Silico Engineering........ 373
Lucy Rutten, Hans de Haard, and Theo Verrips
23 Selection of Human VH Single Domains with Improved Biophysical
Properties by Phage Display...................................... 383
Kip Dudgeon, Romain Rouet, Kristoffer Famm, and Daniel Christ
24 Improvement of Single Domain Antibody Stability by Disulfide
Bond Introduction............................................. 399
Toshihisa Hagihara and Dirk Saerens
Contents ix
2 5 Characterization of Single- Domain Antibodies
with an Engineered Disulfide Bond................................. 417
GregHussack, C. Roger MacKenzie, andjamshid Tanha
26 Affinity Maturation of Single-Domain Antibodies
by Yeast Surface Display......................................... 431
Akiko Koide and Shohei Koide
27 Multivalent Display of Single-Domain Antibodies...................... 445
Jianbing Zhang and C. Roger MacKenzie
28 Methods for Determining the PK Parameters of AlbudAbs™
and of Long Serum Half-Life Drugs Made Using
the AlbudAb™ Technology....................................... 457
Daniel Rycroft and Lucy J. Holt
29 Fluorescent Protein Specific Nanotraps to Study Protein-Protein
Interactions and Histone-Tail Peptide Binding........................ 475
Garwin Fichier, Heinrich Leonhardt, and Ulrich Rothbauer
30 Site-Specific Labeling of His-Tagged Nanobodies with 99mTc:
A Practical Guide.............................................. 485
Catarina Xavier, Nick Devoogdt, Sophie Hernot, Use Vaneycken,
Matthias D Huyvetter, Jens De VOS, Sam Massa, Tony Lahoutte,
and Vicky Caveliers
31 Nanobody-Based Chromatin Immunoprecipitation..................... 491
Trong Nguyen Due, Gholamreza Hassanzadeh-Ghassabeh,
Dirk Saerens, Eveline Peeters, Daniel Charlier, and Serge Muyldermans
32 User-Friendly Expression Plasmids Enable the Fusion of VHHs
to Application-Specific Tags...................................... 507
Ario de Marco
33 Application of Single-Domain Antibodies in Tumor Histochemistry........ 523
Kien T. Maik and C. Roger MacKenzie
Part VI Case Studies
34 Nanobodies as Structural Probes of Protein
Misfolding and Fibril Formation................................... 533
Erwin De Genstand Christopher M. Dobson
35 Molecular Imaging Using Nanobodies: A Case Study................... 559
Nick Devoogdt, Catarina Xavier, Sophie Hernot, Ilse Vaneycken,
Matthias D Huyvetter, Jens De VOS, Sam Massa, Patrick De Baetselier,
Vicky Caveliers, and Tony Lahoutte
36 Case Study on Live Cell Apoptosis-Assay Using Larnin-Chromobody
Cell-Lines for High-Content Analysis............................... 569
Kourosh Zolghadr, Jacqueline Gregor, Heinrich Leonhardt,
and Ulrich Rothbauer
Index........................................................... 577
|
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| indexdate | 2024-07-10T00:24:48Z |
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| language | English |
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| publishDate | 2012 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Single domain antibodies methods and protocols ed. by Dirk Saerens and Serge Muyldermans New York Humana Press 2012 XV, 580 S. Ill., graph. Darst. 27 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 911 Springer protocols Includes bibliographical references and index Antibodies / Laboratory Manuals Monoclonal antibodies / Laboratory manuals Monoklonaler Antikörper (DE-588)4040094-3 gnd rswk-swf Hybridomtechnik (DE-588)4354586-5 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Monoklonaler Antikörper (DE-588)4040094-3 s Hybridomtechnik (DE-588)4354586-5 s b DE-604 Saerens, Dirk Sonstige oth Methods in molecular biology 911 (DE-604)BV035362695 911 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025334426&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Single domain antibodies methods and protocols Methods in molecular biology Antibodies / Laboratory Manuals Monoclonal antibodies / Laboratory manuals Monoklonaler Antikörper (DE-588)4040094-3 gnd Hybridomtechnik (DE-588)4354586-5 gnd |
| subject_GND | (DE-588)4040094-3 (DE-588)4354586-5 (DE-588)4143413-4 |
| title | Single domain antibodies methods and protocols |
| title_auth | Single domain antibodies methods and protocols |
| title_exact_search | Single domain antibodies methods and protocols |
| title_full | Single domain antibodies methods and protocols ed. by Dirk Saerens and Serge Muyldermans |
| title_fullStr | Single domain antibodies methods and protocols ed. by Dirk Saerens and Serge Muyldermans |
| title_full_unstemmed | Single domain antibodies methods and protocols ed. by Dirk Saerens and Serge Muyldermans |
| title_short | Single domain antibodies |
| title_sort | single domain antibodies methods and protocols |
| title_sub | methods and protocols |
| topic | Antibodies / Laboratory Manuals Monoclonal antibodies / Laboratory manuals Monoklonaler Antikörper (DE-588)4040094-3 gnd Hybridomtechnik (DE-588)4354586-5 gnd |
| topic_facet | Antibodies / Laboratory Manuals Monoclonal antibodies / Laboratory manuals Monoklonaler Antikörper Hybridomtechnik Aufsatzsammlung |
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