Suppression of experimental autoimmune-encephalomyelitis by myeloid-derived suppressor cells:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. und engl. Sprache |
| Physical Description: | 100 Bl. Ill. |
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MARC
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| 100 | 1 | |a Sandwick, Sarah |e Verfasser |0 (DE-588)1026271878 |4 aut | |
| 245 | 1 | 0 | |a Suppression of experimental autoimmune-encephalomyelitis by myeloid-derived suppressor cells |c von Sarah Sandwick |
| 246 | 1 | 3 | |a Suppression der Experimentellen Autoimmun-Enzephalomyelitis durch Myeloide Suppressorzellen |
| 264 | 1 | |c 2012 | |
| 300 | |a 100 Bl. |b Ill. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zsfassung in dt. und engl. Sprache | ||
| 502 | |a Würzburg, Univ., Diss., 2012 | ||
| 650 | 0 | 7 | |a Autoaggressionskrankheit |0 (DE-588)4003935-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Suppressorzelle |0 (DE-588)4246406-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Encephalomyelitis |0 (DE-588)4152136-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Myeloblast |0 (DE-588)4307045-0 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Encephalomyelitis |0 (DE-588)4152136-5 |D s |
| 689 | 0 | 1 | |a Autoaggressionskrankheit |0 (DE-588)4003935-3 |D s |
| 689 | 0 | 2 | |a Suppressorzelle |0 (DE-588)4246406-7 |D s |
| 689 | 0 | 3 | |a Myeloblast |0 (DE-588)4307045-0 |D s |
| 689 | 0 | |5 DE-604 | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:20-opus-72690 |
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| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-025289420 | ||
Record in the Search Index
| _version_ | 1804149498075676672 |
|---|---|
| adam_text | IMAGE 1
S A N D W I C K D O C T O R A L T H E S I S , J U N E 2 0 1 2 T A B L E
O F
C O N T E N T
1.1 TABLE O F CONTENT
1 FORWARD 2
1.1 TABLE O F CONTENT 2
1.2 ACKNOWLEDGEMENTS 6
1.3 ABBREVIATIONS 7
1.4 LIST O F FIGURES/TABLES 9
1.5 SUMMARY 11
1.6 ZUSAMMENFASSUNG 13
2 INTRODUCTION 15
2.1 IMMUNE RESPONSES 15
2.1.1 INNATE IMMUNITY 15
2.1.2 ADAPTIVE IMMUNITY 16
2.1.3 IMMUNE ACTIVATION 17
2.1.4 SPLEEN: STRUCTURE AND FUNCTION 18
2.1.5 ADAPTIVE IMMUNE DEVELOPMENT: TOLERANCE 19
2.1.6 TOLERANCE BY ANERGY 20
2.1.7 PROGRAMMED DEATH RECEPTOR-1 AND B7-FAMILY MEMBER HOMOLOGUE-1 20
2.1.8 SUPPRESSION, AS A MODE O F TOLERANCE 21
2.2 MYELOID DERIVED SUPPRESSOR CELLS 22
2.2.1 TRANSCRIPTION FACTORS RELATING TO MDSC 23
2.2.2 MYELOID DERIVED SUPPRESSOR CELL ACTIVATION 23
2.2.3 SUPPRESSIVE CAPACITY O F MDSC 24
2.2.4 MIGRATION O F MDSC 25
2.3 AUTOIMMUNITY 25
2.3.1 MULTIPLE SCLEROSIS: THE DISEASE 26
2.3.2 ANIMAL MODEL FOR MS: EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS 26
2.3.3 MULTIPLE SCLEROSIS: THERAPEUTIC FDA-APPROVED DRUGS 27
2.3.4 TRAFFICKING AND ENTRY INTO CNS 27
2.3.5 SUPPRESSIVE CELLULAR THERAPIES IN EAE 30
2.3.6 MYELOID DERIVED SUPPRESSOR CELLS IN AUTOIMMUNE DISEASES/EAE 30 2.4
SCOPE O F STUDY 31
3 MATERIALS AND METHODS 32
3.1 MATERIALS 32
3.1.1 STIMULI 32
3.1.2 MEDIA, BUFFERS, SOLUTIONS 3 2
3.1.3 GRIESS REAGENT: 34
3.1.4 ANAESTHESIA: 34
3.1.5 IN VIVO TRACKING 3 5
3.1.6 RADIOACTIVE [ 3 H] THYMIDINE 35
3.1.7 EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS 35
3.1.8 ENZYMES AND INHIBITORS 3 6
3.1.9 GM-CSF SUPERNATANT 36
3.1.10 MICE 36
P A G E | 2
HTTP://D-NB.INFO/1026947383
IMAGE 2
S A N D V V I C K D O C T O R A L T H E S I S , J U N E 2 0 1 2 T A B L
E
O F
C O N T E N T
3.2 METHODS 38
3.2.1 HARVESTING, AND CULTURING PRIMARY MURINE CELLS 38
3.2.2 STIMULATION 39
3.2.3 SORTING IMMATURE MYELOID CELL SUBSETS 39
3.2.4 FIXATION O F SUBSETS FOR M L R 40
3.2.5 PROLIFERATION 40
3.2.6 FLOW CYTOMETRY 40
3.2.7 HISTOLOGY BY IMMUNOFLUORESCENCE STAINING 41
3.2.8 CELL TRACKING 41
3.2.9 SOLUBLE FACTORS 42
3.2.10 PCR 42
3.2.11 INDUCTION O F EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS 44
4 RESULTS 45
4.1 CONFIRMING BONE MARROW GENERATION O F MYELOID DERIVED SUPPRESSOR
CELLS AND THEIR CORRESPONDING SPLENIC MYELOID DERIVED SUPPRESSOR CELL
SUBSETS IN UNTREATED WILDTYPE C57B1/6 MICE 45
4.2 CAAT/ENHANCER BINDING PROTEIN-BETA MAY INFLUENCE DEVELOPMENT AND
ACTIVATION O F MONO-BM-MDSC. 46
4.3 INDUCTION O F NITRIC OXIDE BY BONE MARROW GENERATED MYELOID DERIVED
SUPPRESSOR CELLS WITH VARIOUS PATHOGENS AND INTERFERON GAMMA REQUIRES
MYD88, IFN-GRL, AND IRF-1 SIGNALLING, BUT NOT ACTIVATION BY NUCLEIC
ACIDS AND SELF-GLYCOLIPIDS. 48
4.4 INTERFERON-BETA, USED IN MULTIPLE SCLEROSIS DRUG THERAPY, DOES NOT
INFLUENCE MYELOID DERIVED SUPPRESSOR CELL ACTIVATION OR SUPPRESSIVE
CAPACITY. 50 4.5 BOTH IN VITRO GENERATED AND ENDOGENOUS MYELOID DERIVED
SUPPRESSOR CELLS SPECIFICALLY UPREGULATE B7-H1, BUT NO CHANGE OCCURS IN
CO-STIMULATORY
MARKERS ON BOTH SUBSETS 24H AFTER PATHOGEN STIMULATION. 51
4.6 BONE MARROW GENERATED IMMATURE MYELOID CELLS INJECTED INTO MICE
CAUSE SUPPRESSION O F EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYEOLITIS INDUCED
BY MYELIN OLIGODENDROCYTE GLYCOPROTEIN3 5 . 55 . 53
4.7 INCREASED SUPPRESSION O F SPECIFIC MEMORY CELLS BY BONE MARROW
GENERATED IMMATURE MYELOID CELLS IS DEPENDENT UPON THE EXPRESSION O F
PD-1 ON SPLENOCYTES IN VITRO. 56
4.8 BONE MARROW GENERATED IMMATURE MYELOID CELLS EXPRESSION O F B7-H1
DOES NOT CHANGE THE ABILITY TO SUPPRESS EXPERIMENTAL AUTOIMMUNE
ENCEPHALOMYELITIS SEVERITY, BUT PD-1 EXPRESSION IN RECIPIENT MICE DOES.
57 4.9 KINETICS O F BONE MARROW GENERATED IMMATURE MYELOID CELLS
ACCUMULATE IN THE
SPLEEN, HOMING TO THE RED PULP. 58
4.10BONE MARROW GENERATED IMMATURE MYELOID CELLS INDUCE INCREASED SPLEEN
CELLULARITY BY INDUCTION O F PROLIFERATION O F SPLENIC CELLS. 61
5 DISCUSSION 67
5.1 ESTABLISHING THE MODEL O F CELLULAR THERAPY BY MDSC IN EAE 67
5.2 TRANSCRIPTIONAL FACTORS IN MDSC DEVELOPMENT AND ACTIVATION 68
5.3 ACTIVATION REQUIREMENTS O F BM-IMC BY THL AND T H L 7
MICROENVIROMENT STIMULI, IN VITRO 69
5.4 IFN-B TREATMENT DOES NOT DIRECTLY INFLUENCE BM-IMC IN VITRO. 72
5.5 MIGRATION O F BM-MDSC 73
5.6 PROTECTION BY BM-IMC 74
P A G E | 3
IMAGE 3
S A N D W I C K D O C T O R A L T H E S I S , J U N E 2 0 1 2
T A B L E
O F
C O N T E N T
5.7 BM-IMC MAY INDUCE PROLIFERATIVE EFFECTS RATHER THAN SUPPRESSIVE
EFFECTS IN
VIVO 76
6 REFERENCES 78
7 GSLS THESIS GUIDELINES 94
8 CURRICULUM VITAE 96
P A G E | 4
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| any_adam_object | 1 |
| author | Sandwick, Sarah |
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| bvnumber | BV040441594 |
| collection | ebook |
| ctrlnum | (OCoLC)815931593 (DE-599)BVBBV040441594 |
| format | Thesis Book |
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| indexdate | 2024-07-10T00:23:59Z |
| institution | BVB |
| language | English |
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| spelling | Sandwick, Sarah Verfasser (DE-588)1026271878 aut Suppression of experimental autoimmune-encephalomyelitis by myeloid-derived suppressor cells von Sarah Sandwick Suppression der Experimentellen Autoimmun-Enzephalomyelitis durch Myeloide Suppressorzellen 2012 100 Bl. Ill. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache Würzburg, Univ., Diss., 2012 Autoaggressionskrankheit (DE-588)4003935-3 gnd rswk-swf Suppressorzelle (DE-588)4246406-7 gnd rswk-swf Encephalomyelitis (DE-588)4152136-5 gnd rswk-swf Myeloblast (DE-588)4307045-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Encephalomyelitis (DE-588)4152136-5 s Autoaggressionskrankheit (DE-588)4003935-3 s Suppressorzelle (DE-588)4246406-7 s Myeloblast (DE-588)4307045-0 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:20-opus-72690 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-72690 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025289420&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sandwick, Sarah Suppression of experimental autoimmune-encephalomyelitis by myeloid-derived suppressor cells Autoaggressionskrankheit (DE-588)4003935-3 gnd Suppressorzelle (DE-588)4246406-7 gnd Encephalomyelitis (DE-588)4152136-5 gnd Myeloblast (DE-588)4307045-0 gnd |
| subject_GND | (DE-588)4003935-3 (DE-588)4246406-7 (DE-588)4152136-5 (DE-588)4307045-0 (DE-588)4113937-9 |
| title | Suppression of experimental autoimmune-encephalomyelitis by myeloid-derived suppressor cells |
| title_alt | Suppression der Experimentellen Autoimmun-Enzephalomyelitis durch Myeloide Suppressorzellen |
| title_auth | Suppression of experimental autoimmune-encephalomyelitis by myeloid-derived suppressor cells |
| title_exact_search | Suppression of experimental autoimmune-encephalomyelitis by myeloid-derived suppressor cells |
| title_full | Suppression of experimental autoimmune-encephalomyelitis by myeloid-derived suppressor cells von Sarah Sandwick |
| title_fullStr | Suppression of experimental autoimmune-encephalomyelitis by myeloid-derived suppressor cells von Sarah Sandwick |
| title_full_unstemmed | Suppression of experimental autoimmune-encephalomyelitis by myeloid-derived suppressor cells von Sarah Sandwick |
| title_short | Suppression of experimental autoimmune-encephalomyelitis by myeloid-derived suppressor cells |
| title_sort | suppression of experimental autoimmune encephalomyelitis by myeloid derived suppressor cells |
| topic | Autoaggressionskrankheit (DE-588)4003935-3 gnd Suppressorzelle (DE-588)4246406-7 gnd Encephalomyelitis (DE-588)4152136-5 gnd Myeloblast (DE-588)4307045-0 gnd |
| topic_facet | Autoaggressionskrankheit Suppressorzelle Encephalomyelitis Myeloblast Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-72690 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025289420&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT sandwicksarah suppressionofexperimentalautoimmuneencephalomyelitisbymyeloidderivedsuppressorcells AT sandwicksarah suppressionderexperimentellenautoimmunenzephalomyelitisdurchmyeloidesuppressorzellen |