Endogenously expressed bestrophin-1 modulates calcium signaling in the retinal pigmented epithelium:
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| Main Author: | |
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-254798 Inhaltsverzeichnis |
| Physical Description: | XI, 90 Bl. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Endogenously expressed bestrophin-1 modulates calcium signaling in the retinal pigmented epithelium |c vorgelegt von Néstor Más Gómez |
| 264 | 1 | |c 2012 | |
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Record in the Search Index
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| adam_text | IMAGE 1
INDEX
SUMMARY III
ACKNOWLEDGMENTS V
ABBREVIATIONS VIII
1 INTRODUCTION 4
1.1 RPE FUNCTIONS 4
1.1.1 LIGHT ABSORTION 6
1.1.2 EPITHELIAL TRANSPORT 7
1.1.3 SPATIAL ION BUFFERING 8
1.1.4 VISUAL CYCLE 8
1.1.5 PHAGOCYTOSIS 9
1.1.6 SECRETION 9
1.1.7 IMMUNE MODULATION 10
1.2 CALCIUM AND CELL FUNCTION IN GENERAL 10
1.2.1 CALCIUM SIGNALING IN THE RPE 11
1.2.1.1 CALCIUM CHANNELS IN RPE 11
1.3 RPE DISEASES 16
1.3.1 BEST S VITELLIFORM MACULAR DYSTROPHY 16
1.3.1.1 ELECTRO-OCULOGRAM 17
1.4 BESTROPHIN 18
1.5 MUTANT BESTROPHIN-1 21
2 OBJECTIVES OF THE PRESENT WORK 25
3 MATERIAL AND METHODS 26
3.1 MICE MODELS 2 6
3.1.1 MOUSE GENOTYPE 26
3.1.2 LIGH DAMAGE EXPERIMENT 29
1
HTTP://D-NB.INFO/1026355451
IMAGE 2
3.1.2.1 LIGHT DAMAGE HISTOLOGY 29
3.2 PRIMARY MOUSE RETINAL PIGMENTED EPITHELIAL CELLS ISOLATION 29
3.3 PRIMARY PORCINE RETINAL PIGMENTED EPITHELIAL CELLS ISOLATION 30
3.4 RT-PCR 31
3.5 QPCR 32
3.6 SIRNA 33
3.7 WESTERN BLOT 34
3.8 IMMUNOPRECIPITATION AND CO-IMMUNOPRECIPITION 35
3.8.1 PRE-CLEARING 35
3.9 MEASUREMENT OF TRANSEPITHELIAL RESISTANCE 36
3.10 IMMUNOCYTOCHEMISTRY 36
3.11 ELECTRON MICROSCOPY 37
3.12 CALCIUM IMAGING (FURA-2 AM) 38
3.13 STATISTICAL ANALYSIS 39
3.14 EQUIPMENTS 39
4 RESULTS 41
4.1 BESTROPHIN-1 MOUSE MODELS 4 1
4.1.1 LIGHT DAMAGE AT THRESHOLD LEVEL 4 1
4.1.2 LIGHT DAMAGE IN BESTROPHIN-1 I +/Y227N MOUSE MODEL 4 2
4.2 CALCIUM SIGNALLING IN BESTROPHIN-1 MOUSE MODEL 4 3
4.3 PORCINE RETINAL PIGMENTED EPITHELIUM CELL MODEL 45
4.3.1 BESTROPHIN-1 EXPRESSION IN SHORT-TIME PORCINE RPE CELL CULTURE 4 6
4.4 STORE-OPERATED CALCIUM ENTRY (SOCE) IN PORCINE RPE CELLS 50
4.5 BESTROPHIN-1 INFLUENCES SOCE IN PORCINE RPE CELLS 55
5 DISCUSSION 68
5.1 BESTROPHIN-1 MOUSE MODELS 68
5.1.1 LIGHT DAMAGE PARADIGM IN BESTROPHIN-1 KI +,Y227N MOUSE 68
2
IMAGE 3
5.1.2 CALCIUM SIGNALING IN BESTROPHIN-1 KO MOUSE 68
5.2 PORCINE RPE CELL MODEL 69
5.3 STORE-OPERATED CALCIUM ENTRY (SOCE) IN PORCINE RPE CELLS 71
5.3.1 ORAI-1 IS INVOLVED IN SOCE ACTIVATION IN PORCINE RPE CELLS 71
5.4 BESTROPHIN-1 INFLUENCES SOCE PATHWAY IN PORCINE RPE CELLS 74
6 REFERENCES 79
3
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| spelling | Más Gómez, Néstor Verfasser aut Endogenously expressed bestrophin-1 modulates calcium signaling in the retinal pigmented epithelium vorgelegt von Néstor Más Gómez 2012 XI, 90 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Regensburg, Univ., Diss., 2012 Chloridkanal (DE-588)4291189-8 gnd rswk-swf Netzhautdegeneration (DE-588)4171515-9 gnd rswk-swf Pigmentepithel (DE-588)4243854-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Netzhautdegeneration (DE-588)4171515-9 s Pigmentepithel (DE-588)4243854-8 s Chloridkanal (DE-588)4291189-8 s b DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:355-epub-254798 http://epub.uni-regensburg.de/25479/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-254798 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025221383&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Más Gómez, Néstor Endogenously expressed bestrophin-1 modulates calcium signaling in the retinal pigmented epithelium Chloridkanal (DE-588)4291189-8 gnd Netzhautdegeneration (DE-588)4171515-9 gnd Pigmentepithel (DE-588)4243854-8 gnd |
| subject_GND | (DE-588)4291189-8 (DE-588)4171515-9 (DE-588)4243854-8 (DE-588)4113937-9 |
| title | Endogenously expressed bestrophin-1 modulates calcium signaling in the retinal pigmented epithelium |
| title_auth | Endogenously expressed bestrophin-1 modulates calcium signaling in the retinal pigmented epithelium |
| title_exact_search | Endogenously expressed bestrophin-1 modulates calcium signaling in the retinal pigmented epithelium |
| title_full | Endogenously expressed bestrophin-1 modulates calcium signaling in the retinal pigmented epithelium vorgelegt von Néstor Más Gómez |
| title_fullStr | Endogenously expressed bestrophin-1 modulates calcium signaling in the retinal pigmented epithelium vorgelegt von Néstor Más Gómez |
| title_full_unstemmed | Endogenously expressed bestrophin-1 modulates calcium signaling in the retinal pigmented epithelium vorgelegt von Néstor Más Gómez |
| title_short | Endogenously expressed bestrophin-1 modulates calcium signaling in the retinal pigmented epithelium |
| title_sort | endogenously expressed bestrophin 1 modulates calcium signaling in the retinal pigmented epithelium |
| topic | Chloridkanal (DE-588)4291189-8 gnd Netzhautdegeneration (DE-588)4171515-9 gnd Pigmentepithel (DE-588)4243854-8 gnd |
| topic_facet | Chloridkanal Netzhautdegeneration Pigmentepithel Hochschulschrift |
| url | http://epub.uni-regensburg.de/25479/ https://nbn-resolving.org/urn:nbn:de:bvb:355-epub-254798 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025221383&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT masgomeznestor endogenouslyexpressedbestrophin1modulatescalciumsignalingintheretinalpigmentedepithelium |