Histone 3 lysine 4 trimethylation and co-transcriptional splicing: a positive feedback loop reinforcing gene expression
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| Main Author: | |
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 120 S. Ill., graph. Darst. |
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Record in the Search Index
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TABLE OF CONTENTS
S U M M A R Y 6
1 I N T R O D U C T I O N 9
1 . 1 T H E SPL1CEOSOME: A DYNAMIC S N R N P MACHINE 9
1 . 2 CO-TRANSCRIPTIONALITY: N A S C E N T T R A N S C R I P T S A R E
SPLICED DURING TRANSCRIPTION
1 4
1 . 2 . 1 C O U P L I N G OF SPLICING A N D T R A N S C R I P T I O N 1
5
1 . 2 . 2 SPLICING IN T H E CONTEXT OF CHROMATIN 1 7
1 . 3 H 3 K 4 M E 3 , J A R I D 1 DEMETHYLASES A N D SPLICING 2 3
1 . 4 A I M O F T H E T H E S I S 2 6
2 R E S U L T S 2 8
2 . 1 . P E R T U R B I N G HISTONE 3 LYSINE 4 METHYLATION 2 8
2 . 1 . 1 O V E R E X P R E S S I O N OF J A R I D 1 H I S T O N E D E M
E T H Y L A S E S 2 8
2 . 1 . 2 E F F E C T S OF H 3 K 4 M E 3 D E P L E T I O N ON T R A N S
C R I P T I O N 3 0
2 . 1 . 3 E F F E C T S OF H 3 K 4 M E 3 D E P L E T I O N O N SPLICING
3 3
2 . 2 C O - T R A N S C R I P T I O N A L SPLICEOSOME ASSEMBLY 4 0
2 . 2 . 1 M O D E L G E N E S B A S E D O N M O U S E F O S 4 1
2 . 2 . 2 SPLICEOSOME A S S E M B L Y O N M O D E L GENES 4 3
2 . 2 . 3 E F F E C T S OF H 3 K 4 M E 3 DEPLETION ON SPLICEOSOME A S S
E M B L Y 4 8
2 . 3 SPLICING FEEDS BACK ON H 3 K 4 M E 3 5 5
2 . 3 . 1 T H E PRESENCE A N D POSITION OF I N T R O N S INFLUENCES H 3
K 4 M E 3 A N D T R A N S C R I P T I O N . . 5 5
2 . 3 . 2 SPLICING INHIBITION LEADS T O A REDUCTION OF H 3 K 4 M E 3
OVER T H E I N T R O N 6 0
3 D I S C U S S I O N 6 4
3 . 1 J A R I D 1 OVEREXPRESSION DOES N O T LEAD T O GENERAL
TRANSCRIPTIONAL REPRESSION
6 4
3 . 2 H 3 K 4 M E 3 P R O M O T E S CO-TRANSCRIPTIONAL SPLICING 6 5
3 . 3 H 3 K 4 M E 3 D E P E N D S ON SPLICING 7 1
3 . 4 CONCLUDING REMARKS 7 3
4 M E T H O D S 7 5
4 . 1 CELL CULTURE A N D T R E A T M E N T S 7 5
4 . 2 R E D / E T R E C O M B I N E E R I N G 7 5
1
HTTP://D-NB.INFO/1029449295
IMAGE 2
4 . 3 GENERATION OF STABLE H E L A CELL LINES 7 6
4 . 3 . 1 B A C S 7 6
4 . 3 . 2 P L A S M I D S 7 7
4 . 4 S I R N A K N O C K D O W N 7 7
4 . 5 P R I M E R DESIGN A N D EFFICIENCY T E S T FOR Q P C R 7 8
4 . 6 R N A EXTRACTION A N D R T - Q P C R 7 8
4 . 7 GENE EXPRESSION MICROARRAYS 8 0
4 . 8 P R O T E I N EXTRACTION A N D W E S T E R N B L O T A N A L Y S I
S 8 0
4 . 9 CHROMATIN IMMUNOPRECIPITATION A N D Q P C R 8 2
4 . 1 0 MICROSCOPY 8 4
4 . 1 1 FLUORESCENCE RECOVERY A F T E R P H O T O B L E A C H I N G 8 5
5 A P P E N D I X 8 6
6 G L O S S A R Y 1 0 4
7 R E F E R E N C E S 1 0 7
2
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| author | Bieberstein, Nicole 1984- |
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| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.884519 |
| dewey-search | 572.884519 |
| dewey-sort | 3572.884519 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| spelling | Bieberstein, Nicole 1984- Verfasser (DE-588)1024346544 aut Histone 3 lysine 4 trimethylation and co-transcriptional splicing a positive feedback loop reinforcing gene expression von Nicole Bieberstein 2012 120 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Dresden, Techn. Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025218675&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bieberstein, Nicole 1984- Histone 3 lysine 4 trimethylation and co-transcriptional splicing a positive feedback loop reinforcing gene expression |
| subject_GND | (DE-588)4113937-9 |
| title | Histone 3 lysine 4 trimethylation and co-transcriptional splicing a positive feedback loop reinforcing gene expression |
| title_auth | Histone 3 lysine 4 trimethylation and co-transcriptional splicing a positive feedback loop reinforcing gene expression |
| title_exact_search | Histone 3 lysine 4 trimethylation and co-transcriptional splicing a positive feedback loop reinforcing gene expression |
| title_full | Histone 3 lysine 4 trimethylation and co-transcriptional splicing a positive feedback loop reinforcing gene expression von Nicole Bieberstein |
| title_fullStr | Histone 3 lysine 4 trimethylation and co-transcriptional splicing a positive feedback loop reinforcing gene expression von Nicole Bieberstein |
| title_full_unstemmed | Histone 3 lysine 4 trimethylation and co-transcriptional splicing a positive feedback loop reinforcing gene expression von Nicole Bieberstein |
| title_short | Histone 3 lysine 4 trimethylation and co-transcriptional splicing |
| title_sort | histone 3 lysine 4 trimethylation and co transcriptional splicing a positive feedback loop reinforcing gene expression |
| title_sub | a positive feedback loop reinforcing gene expression |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025218675&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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