Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei: strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2011
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Getr. Zählung Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV040362864 | ||
| 003 | DE-604 | ||
| 005 | 20161123 | ||
| 007 | t | ||
| 008 | 120813s2011 ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)815887219 | ||
| 035 | |a (DE-599)BSZ368149501 | ||
| 040 | |a DE-604 |b ger | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-188 |a DE-29T |a DE-12 |a DE-703 | ||
| 082 | 0 | |a 610 | |
| 084 | |a XX 4200 |0 (DE-625)153080:12905 |2 rvk | ||
| 100 | 1 | |a Cupelli, Anna Karolina |d 1981- |e Verfasser |0 (DE-588)1024211169 |4 aut | |
| 245 | 1 | 0 | |a Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei |b strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B |c vorgelegt von Anna Karolina Cupelli |
| 264 | 1 | |c 2011 | |
| 300 | |a Getr. Zählung |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Tübingen, Univ., Diss., 2011 | ||
| 650 | 0 | 7 | |a Adenoviren |0 (DE-588)4141412-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Trypanosoma brucei |0 (DE-588)4186350-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Kathepsin B |0 (DE-588)4339104-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Zellrezeptor |0 (DE-588)4133853-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Adenoviren |0 (DE-588)4141412-3 |D s |
| 689 | 0 | 1 | |a Zellrezeptor |0 (DE-588)4133853-4 |D s |
| 689 | 0 | 2 | |a Trypanosoma brucei |0 (DE-588)4186350-1 |D s |
| 689 | 0 | 3 | |a Kathepsin B |0 (DE-588)4339104-7 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025216678&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-025216678 | ||
Datensatz im Suchindex
| _version_ | 1804149408684572673 |
|---|---|
| adam_text | IMAGE 1
TABLE OF CONTENTS
1 INTRODUCTION: PART I 1
1.1 VIRUSES AND VIRAL INFECTION 1
1.2 VIRUS CLASSIFICATION AND VIRUS STRUCTURE 3
1.3 HUMAN ADENOVIRUSES (HADS) 5
1.4 THERAPEUTIC RELEVANCE OF HUMAN ADENOVIRUSES 5
1.5 INFECTION CYCLE 8
1.5.1 ATTACHMENT AND INTERNALIZATION 8
1.5.2 DISSASEMBLY AND RELEASE 8
1.6 ADENOVIRUS STRUCTURE 11
1.7 ATTACHMENT PROTEIN: FIBER (PROTEIN IV) 12
1.8 INTERNALIZATION PROTEIN: PENTON BASE (PROTEIN III) 13
1.9 ATTACHMENT RECEPTORS 15
1.9.1 MEMBRANE COFACTOR PROTEIN CD46 15
1.9.2 CAR 17
1.9.3 GD1A 18
1.9.4 DSG-2 18
1.10 ENTRY RECEPTORS: INTEGRINS 19
1.10.1 INTEGRIN FUNCTION 19
1.10.2 INTEGRIN STRUCTURE 24
2 INTRODUCTION: PART II 26
2.1 TRYPANOSOMIASIS AND TRYPANOSOMA BRUCEI 26
2.1.1 MORPHOLOGY AND LIFE CYCLE 26
2.1.2 TRANSMISSION AND PATHOGENICITY 29
2.1.3 TREATMENT 30
2.1.4 AUTOPHAGY AND CYSTEINE PROTEASES 30
2.2 TRYPANOSOMA BRUCEI CATHEPSIN B 31
2.2.1 FUNCTION AND STRUCTURE 31
2.2.2 REACTION MECHANISM 34
2.2.3 INHIBITION 35
HTTP://D-NB.INFO/1024899144
IMAGE 2
3 METHODS 36
3.1 PROTEIN CRYSTALLOGRAPHY 36
3.1.1 CRYSTAL LATTICE AND CRYSTAL SYMMETRY 37
3.1.2 NON-CRYSTALLOGRAPHIC SYMMETRY 37
3.1.3 PROTEIN CRYSTALLIZATION 38
3.2 X-RAY STRUCTURE ANALYSIS 39
3.2.1 THE PRINCIPLE OF X-RAY DIFFRACTION 39
3.2.2 TEMPERATURE FACTOR 41
3.2.3 RECIPROCAL SPACE AND EWALD CONSTRUCTION 42
3.2.4 DATA PROCESSING AND DATA REDUCTION 44
3.2.5 DATA VALIDATION 45
3.2.6 PHASE DETERMINATION 46
3.2.7 REFINEMENT 49
3.2.8 REFINEMENT VALIDATION 53
3.3 FREE ELECTRON LASER AND FEMTOSECOND X-RAY CRYSTALLOGRAPHY.... 54
3.3.1 GENERATION OF FEMTOSECOND X-RAY PULSES 55
3.3.2 DATA COLLECTION BEFORE DESTRUCTION 56
4 AIMS 58
4.1 HUMAN ADENOVIRUS-HOST CELL RECEPTOR INTERACTIONS 58
4.2 TRYPANOSOMA BRUCEI CYSTEINE PROTEASE CATHEPSIN B 60
5 RESULTS AND DISCUSSION 61
5.1 STRUCTURE OF ADENOVIRUS TYPE 21 KNOB IN COMPLEX WITH
CD46 REVEALS KEY DIFFERENCES IN RECEPTOR CONTACTS AMONG SPECIES B
ADENOVIRUSES 61
5.2 VIRAL ATTACHMENT STRATEGIES: THE MANY FACES OF ADENOVIRUSES.. 65
5.3 IN VIVO CRYSTALLIZATION AND SERIAL FEMTOSECOND CRYSTALLOGRAPHY OF
CATHEPSIN B FROM THE PROTOZOAN PARASITE TRYPANOSOMA BRUCEI 68
6 ONGOING RESEARCH 75
6.1 EXPRESSION OF OVP 3 76
6.2 PURIFICATION OF A VP3 7 7
IMAGE 3
6.3 EXPRESSION AND PURIFICATION OF HUMAN ADENOVIRUS TYPE 9 PENTON
BASE 79
6.4 AFFINITY MEASUREMENTS 79
6.5 STRUCTURAL ANALYSES 82
7 CONCLUSIONS AND OUTLOOK 85
8 BIBLIOGRAPHY 87
9 APPENDIX 102 ^
9.1 CURRICULUM VITAE 102
9.2 ACKNOWLEDGMENTS 104
|
| any_adam_object | 1 |
| author | Cupelli, Anna Karolina 1981- |
| author_GND | (DE-588)1024211169 |
| author_facet | Cupelli, Anna Karolina 1981- |
| author_role | aut |
| author_sort | Cupelli, Anna Karolina 1981- |
| author_variant | a k c ak akc |
| building | Verbundindex |
| bvnumber | BV040362864 |
| classification_rvk | XX 4200 |
| ctrlnum | (OCoLC)815887219 (DE-599)BSZ368149501 |
| dewey-full | 610 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 610 - Medicine and health |
| dewey-raw | 610 |
| dewey-search | 610 |
| dewey-sort | 3610 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01832nam a2200409 c 4500</leader><controlfield tag="001">BV040362864</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20161123 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">120813s2011 ad|| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)815887219</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BSZ368149501</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-188</subfield><subfield code="a">DE-29T</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-703</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">610</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">XX 4200</subfield><subfield code="0">(DE-625)153080:12905</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Cupelli, Anna Karolina</subfield><subfield code="d">1981-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1024211169</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei</subfield><subfield code="b">strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B</subfield><subfield code="c">vorgelegt von Anna Karolina Cupelli</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2011</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Getr. Zählung</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Tübingen, Univ., Diss., 2011</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Adenoviren</subfield><subfield code="0">(DE-588)4141412-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Trypanosoma brucei</subfield><subfield code="0">(DE-588)4186350-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Kathepsin B</subfield><subfield code="0">(DE-588)4339104-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Zellrezeptor</subfield><subfield code="0">(DE-588)4133853-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Adenoviren</subfield><subfield code="0">(DE-588)4141412-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Zellrezeptor</subfield><subfield code="0">(DE-588)4133853-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Trypanosoma brucei</subfield><subfield code="0">(DE-588)4186350-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Kathepsin B</subfield><subfield code="0">(DE-588)4339104-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025216678&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-025216678</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV040362864 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:22:30Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025216678 |
| oclc_num | 815887219 |
| open_access_boolean | |
| owner | DE-188 DE-29T DE-12 DE-703 |
| owner_facet | DE-188 DE-29T DE-12 DE-703 |
| physical | Getr. Zählung Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| record_format | marc |
| spelling | Cupelli, Anna Karolina 1981- Verfasser (DE-588)1024211169 aut Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B vorgelegt von Anna Karolina Cupelli 2011 Getr. Zählung Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Tübingen, Univ., Diss., 2011 Adenoviren (DE-588)4141412-3 gnd rswk-swf Trypanosoma brucei (DE-588)4186350-1 gnd rswk-swf Kathepsin B (DE-588)4339104-7 gnd rswk-swf Zellrezeptor (DE-588)4133853-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Adenoviren (DE-588)4141412-3 s Zellrezeptor (DE-588)4133853-4 s Trypanosoma brucei (DE-588)4186350-1 s Kathepsin B (DE-588)4339104-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025216678&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cupelli, Anna Karolina 1981- Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B Adenoviren (DE-588)4141412-3 gnd Trypanosoma brucei (DE-588)4186350-1 gnd Kathepsin B (DE-588)4339104-7 gnd Zellrezeptor (DE-588)4133853-4 gnd |
| subject_GND | (DE-588)4141412-3 (DE-588)4186350-1 (DE-588)4339104-7 (DE-588)4133853-4 (DE-588)4113937-9 |
| title | Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B |
| title_auth | Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B |
| title_exact_search | Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B |
| title_full | Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B vorgelegt von Anna Karolina Cupelli |
| title_fullStr | Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B vorgelegt von Anna Karolina Cupelli |
| title_full_unstemmed | Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B vorgelegt von Anna Karolina Cupelli |
| title_short | Structural investigations of Adenovirus-host cell receptor interactions and the Cathepsin B protease from Trypanosoma brucei |
| title_sort | structural investigations of adenovirus host cell receptor interactions and the cathepsin b protease from trypanosoma brucei strukturelle untersuchungen von adenovirus rezeptor wechselwirkungen und der trypanosomalen protease cathepsin b |
| title_sub | strukturelle Untersuchungen von Adenovirus-Rezeptor-Wechselwirkungen und der Trypanosomalen Protease Cathepsin B |
| topic | Adenoviren (DE-588)4141412-3 gnd Trypanosoma brucei (DE-588)4186350-1 gnd Kathepsin B (DE-588)4339104-7 gnd Zellrezeptor (DE-588)4133853-4 gnd |
| topic_facet | Adenoviren Trypanosoma brucei Kathepsin B Zellrezeptor Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025216678&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT cupelliannakarolina structuralinvestigationsofadenovirushostcellreceptorinteractionsandthecathepsinbproteasefromtrypanosomabruceistrukturelleuntersuchungenvonadenovirusrezeptorwechselwirkungenunddertrypanosomalenproteasecathepsinb |