Differential gene expression during pre-implantation pregnancy in Bos taurus:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | kostenfrei https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120625-1096646-1-9 Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache |
| Beschreibung: | IX, 94 Bl. graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Differential gene expression during pre-implantation pregnancy in Bos taurus |c Andréa Hammerle-Fickinger |
| 264 | 1 | |c 2012 | |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
TABLE OF CONTENTS
LIST OF ABBREVIATIONS IV
ZUSAMMENFASSUNG VI
ABSTRACT VIII
1 INTRODUCTION 1
1.1 BOVINE ESTROUS CYCLE 1
1.2 ACTIVATION OF THE COMPLEMENT SYSTEM DURING PREGNANCY 2
1.3 EARLY PREGNANCY IN CATTLE AND SIGNALING RESPONSES 4
1.4 DIAPEDESIS: LEUKOCYTES MIGRATION FROM BLOOD INTO MILK 7
1.5 GENE EXPRESSION ANALYSIS AS A POTENTIAL METHOD FOR PREGNANCY
DIAGNOSIS IN CATTLE.... 8
1.6 IMPORTANCE OF SAMPLE PROCESSING FOR GENE EXPRESSION ANALYSIS 9
1.7 AIM OF THE THESIS 11
2 MATERIAL AND METHODS 13
2.1 STUDY DESIGN OF PREGNANCY RECOGNITION REGARDING THE COMPLEMENT
SYSTEM ACTIVATION IN
ENDOMETRIUM 13
2.1.1 MONOZYGOTIC TWIN ANIMALS STUDY 13
2.1.2 CYCLIC ANIMALS STUDY 13
2.1.3 IN VITRO CO-CULTURE OF GLANDULAR EPITHELIAL AND STROMA CELLS STUDY
14
2.1.4 TISSUE SAMPLING AND RNA EXTRACTION 14
2.2 STUDY DESIGN OF PREGNANCY RECOGNITION IN PERIPHERAL BLOOD LEUKOCYTES
14
2.2.1 VALIDATION OF BLOOD SAMPLING TECHNIQUES 14
2.2.2 BLOOD LEUKOCYTES STUDY 16
2.3 STUDY DESIGN OF PREGNANCY RECOGNITION IN MILK SOMATIC CELLS 18
2.3.1 VALIDATION OF MILK SAMPLING TECHNIQUES 18
2.3.2 MILK SOMATIC CELLS STUDY 19
HTTP://D-NB.INFO/102967132X
IMAGE 2
TABLE OF CONTENTS
2.4 RNA CONCENTRATION AND QUALITY DETERMINATION 20
2.5 TARGET GENE SELECTION AND PRIMER DESIGN 20
2.6 QUANTITATIVE RT-PCR 23
2.6.1 GENE EXPRESSION ANALYSIS IN ENDOMETRIUM 23
2.6.2 GENE EXPRESSION ANALYSIS FOR VALIDATION OF BLOOD SAMPLING 23
2.6.3 GENE EXPRESSION ANALYSIS ON BLOOD LEUKOCYTES AND ON MILK SOMATIC
CELLS 24
2.6.4 STATISTICAL ANALYSIS OF GENE EXPRESSION DATA 25
2.7 MICROARRAY ANALYSIS 26
2.7.1 GENE EXPRESSION ANALYSIS IN ENDOMETRIUM 26
2.7.2 GENE EXPRESSION ANALYSIS ON BLOOD LEUKOCYTES 26
2.8 DETERMINATION OF C1Q CONCENTRATION BY ELISA 27
3 RESULTS 28
3.1 ANALYSIS OF PREGNANCY RECOGNITION REGARDING THE COMPLEMENT SYSTEM
ACTIVATION IN
ENDOMETRIUM 28
3.1.1 EXPRESSION OF COMPLEMENT SYSTEM FACTORS BY RT-QPCR 28
3.1.2 EXPRESSION OF COMPLEMENT SYSTEM FACTORS BY MICROARRAY ANALYSIS 29
3.1.3 EXPRESSION OF COMPLEMENT SYSTEM FACTORS IN AN IN VITRO CO-CULTURE
SYSTEM 29
3.2 ANALYSIS OF PREGNANCY RECOGNITION IN PERIPHERAL BLOOD LEUKOCYTES 31
3.2.1 VALIDATION OF BLOOD SAMPLING TECHNIQUES 31
3.2.2 IDENTIFICATION OF A GENE EXPRESSION SIGNATURE IN BOVINE BLOOD
LEUKOCYTES 37
3.3 ANALYSIS OF PREGNANCY RECOGNITION IN MILK SOMATIC CELLS 45
3.3.1 VALIDATION OF MILK SAMPLING TECHNIQUES 45
3.3.2 IDENTIFICATION OF A GENE EXPRESSION SIGNATURE IN BOVINE MILK
SOMATIC CELLS 50
4 DISCUSSION 55
4.1 ANALYSIS OF PREGNANCY RECOGNITION REGARDING THE COMPLEMENT SYSTEM
ACTIVATION IN
ENDOMETRIUM 55
II
IMAGE 3
TABLE OF CONTENTS
4.2 ANALYSIS OF PREGNANCY RECOGNITION IN PERIPHERAL BLOOD LEUKOCYTES 58
4.3 ANALYSIS OF PREGNANCY RECOGNITION IN MILK SOMATIC CELLS 64
5 CONCLUSIONS AND PERSPECTIVES 69
6 REFERENCES 73
ACKNOWLEDGEMENTS 84
SCIENTIFIC COMMUNICATION 85
CURRICULUM VITAE 87
APPENDIX 88
|
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| ctrlnum | (OCoLC)802791121 (DE-599)BVBBV040339056 |
| discipline | Chemie Agrarwissenschaft Veterinärmedizin |
| format | Thesis Book |
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| spelling | Hammerle-Fickinger, Andréa Verfasser aut Differential gene expression during pre-implantation pregnancy in Bos taurus Andréa Hammerle-Fickinger 2012 IX, 94 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache München, Techn. Univ., Diss., 2012 Immunreaktion (DE-588)4133841-8 gnd rswk-swf Rind (DE-588)4050061-5 gnd rswk-swf Trächtigkeitsnachweis (DE-588)4185810-4 gnd rswk-swf Präimplantationsphase (DE-588)4293796-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Rind (DE-588)4050061-5 s Trächtigkeitsnachweis (DE-588)4185810-4 s Präimplantationsphase (DE-588)4293796-6 s Immunreaktion (DE-588)4133841-8 s b DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:91-diss-20120625-1096646-1-9 http://mediatum.ub.tum.de/node?id=1096646 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120625-1096646-1-9 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025193351&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hammerle-Fickinger, Andréa Differential gene expression during pre-implantation pregnancy in Bos taurus Immunreaktion (DE-588)4133841-8 gnd Rind (DE-588)4050061-5 gnd Trächtigkeitsnachweis (DE-588)4185810-4 gnd Präimplantationsphase (DE-588)4293796-6 gnd |
| subject_GND | (DE-588)4133841-8 (DE-588)4050061-5 (DE-588)4185810-4 (DE-588)4293796-6 (DE-588)4113937-9 |
| title | Differential gene expression during pre-implantation pregnancy in Bos taurus |
| title_auth | Differential gene expression during pre-implantation pregnancy in Bos taurus |
| title_exact_search | Differential gene expression during pre-implantation pregnancy in Bos taurus |
| title_full | Differential gene expression during pre-implantation pregnancy in Bos taurus Andréa Hammerle-Fickinger |
| title_fullStr | Differential gene expression during pre-implantation pregnancy in Bos taurus Andréa Hammerle-Fickinger |
| title_full_unstemmed | Differential gene expression during pre-implantation pregnancy in Bos taurus Andréa Hammerle-Fickinger |
| title_short | Differential gene expression during pre-implantation pregnancy in Bos taurus |
| title_sort | differential gene expression during pre implantation pregnancy in bos taurus |
| topic | Immunreaktion (DE-588)4133841-8 gnd Rind (DE-588)4050061-5 gnd Trächtigkeitsnachweis (DE-588)4185810-4 gnd Präimplantationsphase (DE-588)4293796-6 gnd |
| topic_facet | Immunreaktion Rind Trächtigkeitsnachweis Präimplantationsphase Hochschulschrift |
| url | http://mediatum.ub.tum.de/node?id=1096646 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120625-1096646-1-9 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025193351&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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