Biocatalysts and enzyme technology:
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| Main Authors: | , , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Weinheim
Wiley-Blackwell
2012
|
| Edition: | 2., completely rev., and enl. ed. |
| Subjects: | |
| Online Access: | Inhaltstext Inhaltsverzeichnis |
| Physical Description: | XX, 606 S. Ill., graph. Darst. 240 mm x 170 mm |
| ISBN: | 9783527329892 9783527632916 |
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MARC
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| 100 | 1 | |a Buchholz, Klaus |d 1941- |e Verfasser |0 (DE-588)118071696 |4 aut | |
| 245 | 1 | 0 | |a Biocatalysts and enzyme technology |c Klaus Buchholz, Volker Kasche, and Uwe T. Bornscheuer |
| 250 | |a 2., completely rev., and enl. ed. | ||
| 264 | 1 | |a Weinheim |b Wiley-Blackwell |c 2012 | |
| 300 | |a XX, 606 S. |b Ill., graph. Darst. |c 240 mm x 170 mm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
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| 700 | 1 | |a Kasche, Volker |d 1939-2017 |e Verfasser |0 (DE-588)11807170X |4 aut | |
| 700 | 1 | |a Bornscheuer, Uwe Theo |d 1964- |e Verfasser |0 (DE-588)121061841 |4 aut | |
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Record in the Search Index
| _version_ | 1826212228886429696 |
|---|---|
| adam_text |
I
V
I
I
CONTENTS
PREFACE TO THE SECOND EDITION XV
PREFACE TO THE FIRST GERMAN EDITION XVII
PREFACE TO THE FIRST ENGLISH EDITION XX
1 INTRODUCTION TO ENZYME TECHNOLOGY 1
1.1 INTRODUCTION 1
1.1.1 WHAT ARE BIOCATALYSTS? 2
1.1.2 BIO- AND CHEMOCATALYSTS - SIMILARITIES AND DIFFERENCES 2
1.2 GOALS AND POTENTIAL OF BIOTECHNOLOGICAL PRODUCTION PROCESSES 4
1.3 HISTORICAL HIGHLIGHTS O
F
ENZYME TECHNOLOGY/APPLIED BIOCATALYSIS
8
1.3.1 EARLY DEVELOPMENTS
8
1.3.2 SCIENTIFIC PROGRESS SINCE 1890: THE BIOCHEMICAL PARADIGM; GROWING
SUCCESS IN APPLICATION 10
1.3.3 DEVELOPMENTS SINCE 1950 13
1.4 BIOTECHNOLOGICAL PROCESSES: THE USE O
F
ISOLATED OR INTRACELLULAR
ENZYMES AS BIOCATALYSTS 15
1.5 ADVANTAGES AND DISADVANTAGES O
F
ENZYME-BASED PRODUCTION
PROCESSES 19
1.6 GOALS AND ESSENTIAL SYSTEM PROPERTIES FOR NEW OR IMPROVED ENZYME
PROCESSES 22
1.6.1 GOALS 22
1.6.2 ESSENTIAL SYSTEM PROPERTIES FOR RATIONAL DESIGN OF AN ENZYME
PROCESS 24
1.6.3 CURRENT USE AND POTENTIAL O
F
ENZYME TECHNOLOGY 27
EXERCISES 28
LITERATURE 29
REFERENCES 29
2 BASICS O
F
ENZYMES AS BIOCATALYSTS 33
2.1 INTRODUCTION 34
2.2 ENZYME CLASSIFICATION 35
2.3 ENZYME SYNTHESIS AND STRUCTURE 37
2.4 ENZYME FUNCTION AND ITS GENERAL MECHANISM 41
HTTP://D-NB.INFO/1021678481
V
I
I
I I
CONTENTS
2.5 FREE ENERGY CHANGES AND THE SPECIFICITY OF ENZYME-CATALYZED
REACTIONS 52
2.6 EQUILIBRIUM- AND KINETICALLY CONTROLLED REACTIONS CATALYZED BY
ENZYMES 54
2.7 KINETICS OF ENZYME-CATALYZED REACTIONS 58
2.7.1 QUANTITATIVE RELATIONS FOR KINETIC CHARACTERISTICS AND
SELECTIVITIES OF
ENZYME-CATALYZED REACTIONS 59
2.7.1.1 TURNOVER NUMBER (K
CAT
) AND MICHAELIS-MENTEN CONSTANT (K
M
) 59
2.7.1.2 STEREOSELECTIVITIES FOR EQUILIBRIUM- AND KINETICALLY CONTROLLED
REACTIONS 63
2.7.2 DEPENDENCE O
F
K
CAT
, K
M
, AND SELECTIVITIES ON PH, TEMPERATURE,
INHIBITORS, ACTIVATORS, AND IONIC STRENGTH IN AQUEOUS
SOLUTIONS 68
2.7.2.1 PH DEPENDENCE 69
2H.2.2 TEMPERATURE DEPENDENCE 71
2.7.2.3 BINDING OF ACTIVATOR AND INHIBITOR MOLECULES 73
2.7.2.4 INFLUENCE O
F
IONIC STRENGTH 75
2.8 END POINTS O
F
ENZYME PROCESSES AND AMOUNT OF ENZYME REQUIRED TO
REACH THE END POINT IN A GIVEN TIME 76
2.8.1 TEMPERATURE DEPENDENCE O
F
THE PRODUCT YIELD 79
2.8.2 P
H
DEPENDENCE OF THE YIELD AT THE END POINT 79
2.8.3 END POINTS FOR KINETIC RESOLUTIONS O
F
RACEMATES 82
2.9 ENZYME-CATALYZED PROCESSES WITH SLIGHTLY SOLUBLE PRODUCTS AND
SUBSTRATES 83
2.9.1 ENZYME-CATALYZED PROCESSES IN AQUEOUS SUSPENSIONS 84
2.9.1.1 CHANGES IN RATES, K
CAT
, K
M
, AND SELECTIVITIES IN THESE SYSTEMS
COMPARED WITH HOMOGENEOUS AQUEOUS SOLUTIONS 85
2.9.2 ENZYME-CATALYZED PROCESSES IN NONCONVENTIONAL SOLVENTS
WHERE PRODUCTS AND SUBSTRATES ARE DISSOLVED (AND THE ENZYME
SUSPENDED) 85
2.9.2.1 CHANGES IN RATES, K
CAT
, K
M
, AND SELECTIVITIES IN THESE SYSTEMS
COMPARED WITH HOMOGENEOUS AQUEOUS SOLUTIONS 89
2.10 STABILITY, DENATURATION, AND RENATURATION O
F
ENZYMES 91
2.11 BETTER ENZYMES BY NATURAL EVOLUTION, IN VITRO EVOLUTION, OR
RATIONAL
ENZYME ENGINEERING 94
2.11.1 CHANGES IN ENZYME PROPERTIES BY NATURAL EVOLUTION 96
2.11.1.1 FC
CAT
AND K
M
96
2.11.1.2 ENZYME STABILITY 99
2.11.1.3 STEREOSELECTIVITY 100
2.11.1.4 SELECTIVITY IN KINETICALLY CONTROLLED SYNTHESIS OF CONDENSATION
PRODUCTS 100
EXERCISES 101
LITERATURE 105
REFERENCES 106
CONTENTS
IIX
3 ENZYME DISCOVERY AND PROTEIN ENGINEERING 111
3.1 ENZYME DISCOVERY 111
3.2 STRATEGIES FOR PROTEIN ENGINEERING 115
3.2.1 RATIONAL PROTEIN DESIGN 117
3.2.2 DIRECTED (MOLECULAR) EVOLUTION 118
3.2.2.1 METHODS TO CREATE MUTANT LIBRARIES 118
3.2.2.2 ASSAY SYSTEMS 121
3.2.2.3 EXAMPLES 124
3.2.3 FOCUSED DIRECTED EVOLUTION 129
3.3 COMPUTATIONAL DESIGN O
F
ENZYMES 131
EXERCISES 132
REFERENCES 132
4 ENZYMES IN ORGANIC CHEMISTRY 141
4.1 INTRODUCTION 141
4.1.1 KINETIC RESOLUTION OR ASYMMETRIC SYNTHESIS 143
4.2 EXAMPLES 144
4.2.1 OXIDOREDUCTASES (EC 1) 144
4.2.1.1 DEHYDROGENASES (EC 1.1.1.-, EC 1.2.1.-, EC 1.4.1.-) 144
4.2.1.2 OXYGENASES 148
4.2.1.3 PEROXIDASES (EC 1.11.1.10) 157
4.2.1.4 ENOATE REDUCTASES (EC 1.4.1.31) 157
4.2.1.5 MONOAMINE OXIDASES 159
4.2.2 TRANSAMINASES 161
4.2.3 HYDROLASES (EC 3.1) 165
4.2.3.1 LIPASES (EC 3.1.1.3) 165
4.2.3.2 ESTERASES (EC 3.1.1.1) 172
4.2.3.3 PEPTIDASES, ACYLASES, AND AMIDASES 176
4.2.3.4 EPOXIDE HYDROLASES (EC 3.3.2.3) 178
4.2.3.5 DEHALOGENASES (EC 3.8.1.5) 182
4.2.3.6 NITRILASES (EC 3.5.5.1) AND NITRILE HYDRATASES (EC 4.2.1.84) 182
4.2.3.7 HYDANTOINASES (EC 3.5.2.-) 187
4.2.4 LYASES (EC 4) 189
4.2.4.1 HYDROXYNITRILE LYASES (EC 4.1.2.-) 189
4.2.4.2 ALDOLASES (EC 4.1.2.-, EC 4.1.3.-) 193
4.2.5 ISOMERASES (EC 5) 197
EXERCISES 199
LITERATURE 199
REFERENCES 199
5 CELLS DESIGNED BY METABOLIC ENGINEERING AS BIOCATALYSTS
FOR MULTIENZYME BIOTRANSFORMATIONS 209
5.1 INTRODUCTION 209
5.2 A SHORT INTRODUCTION TO METABOLIC ENGINEERING 210
5.3 EXAMPLES 214
X
| CONTENTS
5.3.1 1,3-PROPANEDIOL 214
5.3.2 SYNTHESIS O
F
"BIODIESEL" AND OTHER FATTY ACID
DERIVATIVES 215
5.3.3 CONVERSION OF CELLULOSICS TO ETHANOL 216
5.3.4 CONVERSION OF
D-FRUCTOSE
TO
D-MANNITOL
218
5.3.5 SYNTHESIS OF L-ASCORBIC ACID 219
5.3.6 OTHER EXAMPLES 220
EXERCISES 222
LITERATURE 222
REFERENCES 222
6
ENZYME PRODUCTION AND PURIFICATION 225
6.1 INTRODUCTION 226
6.2 ENZYME SOURCES 227
6.2.1 ANIMAL AND PLANT TISSUES 227
6.2.2 WILD-TYPE MICROORGANISMS 229
6.2.3 RECOMBINANT MICROORGANISMS 231
6.3 IMPROVING ENZYME YIELD 231
6.3.1 PROCESSES THAT INFLUENCE THE ENZYME YIELD 233
6.4 INCREASING THE YIELD OF PERIPLASMIC AND EXTRACELLULAR
ENZYMES 236
6.4.1 PENICILLIN AMIDASE 238
6.4.2 LIPASE 243
6.5 DOWNSTREAM PROCESSING OF ENZYMES 245
6.5.1 STATIC AND DYNAMIC PROPERTIES OF CHROMATOGRAPHIC ADSORBENTS THAT
MUST BE KNOWN FOR A RATIONAL DESIGN OF CHROMATOGRAPHIC PROTEIN
PURIFICATION 249
6.5.1.1 STATIC PROPERTIES 249
6.5.1.2 DYNAMIC PROPERTIES 252
6.5.2 CHROMATOGRAPHIC PURIFICATION OF ENZYMES: PROBLEMS AND
PROCEDURES 255
6.5.2.1 PROBLEMS 255
6.5.2.2 PROCEDURES 255
6.5.3 CHROMATOGRAPHIC PURIFICATION AND CONDITIONING O
F
TECHNICAL AND
THERAPEUTIC ENZYMES 257
6.5.3.1 TECHNICAL ENZYMES 257
6.5.3.2 ENZYMES FOR THERAPY AND DIAGNOSTICS 259
6.6 REGULATIONS BASED ON RISK ASSESSMENTS/SAFETY CRITERIA THAT INFLUENCE
THE PRODUCTION OF ENZYMES AND THEIR USE FOR ANALYTICAL,
PHARMACEUTICAL, SCIENTIFIC, AND TECHNICAL PURPOSES 260
6.6.1 REGULATIONS GOVERNING THE USE OF GENETICALLY MODIFIED
MICROORGANISMS FOR THE PRODUCTION O
F
ENZYMES IN LABORATORIES AND
PRODUCTION FACILITIES 260
6.6.2 REGULATIONS GOVERNING THE USE OF ENZYMES PRODUCED IN WILD-TYPE OR
RECOMBINANT ORGANISMS 265
CONTENTS I X
I
EXERCISES 267
LITERATURE 268
REFERENCES 269
7 APPLICATION O
F
ENZYMES IN SOLUTION: SOLUBLE ENZYMES AND ENZYME
SYSTEMS 275
7.1 INTRODUCTION AND AREAS OF APPLICATION 276
7.1.1 THE IMPACT OF GENETIC ENGINEERING 278
7.1.2 MEDIUM DESIGN 279
7.1.3 SAFETY ASPECTS 280
7.2 SPACE-TIME YIELD AND PRODUCTIVITY 281
7.3 EXAMPLES FOR THE APPLICATION O
F
ENZYMES IN SOLUTION 286
7.3.1 SURVEY 286
7.3.1.1 FOOD APPLICATIONS 289
7.3.1.2 OTHER INDUSTRIAL APPLICATIONS 290
7.3.2 STARCH PROCESSING 291
7.3.3 DETERGENTS 294
7.4 MEMBRANE SYSTEMS AND PROCESSES 297
EXERCISES 305
LITERATURE 307
REFERENCES 308
8 IMMOBILIZATION O
F
ENZYMES (INCLUDING APPLICATIONS) 313
8.1 PRINCIPLES 313
8.1.1 PARAMETERS O
F
IMMOBILIZATION 317
8.2 CARRIERS 319
8.2.1 INORGANIC CARRIERS 321
8.2.2 POLYSACCHARIDES 321
8.2.3 SYNTHETIC POLYMERS 326
8.3 BINDING METHODS 330
8.3.1 ADSORPTION 330
8.3.2 COVALENT BINDING 331
8.4 EXAMPLES: APPLICATION OF IMMOBILIZED ENZYMES 335
8.4.1 HYDROLYSIS AND BIOTRANSFORMATION OF CARBOHYDRATES 335
8.4.2 HYDROLYSIS AND SYNTHESIS OF PENICILLINS AND CEPHALOSPORINS 345
8.4.3 FURTHER PROCESSES 346
8.4.3.1 AMINO ACID, PEPTIDE, AND AMIDE SYNTHESIS 346
8.4.3.2 APPLICATION OF LIPASES 348
EXERCISES 349
LITERATURE 352
REFERENCES 352
9 IMMOBILIZATION O
F
MICROORGANISMS AND CELLS 359
9.1 INTRODUCTION 359
9.2 FUNDAMENTAL ASPECTS 362
X
I
I |
CONTENTS
9.3 IMMOBILIZATION BY AGGREGATION/FLOCCULATION 364
9.4 IMMOBILIZATION BY ENTRAPMENT 368
9.4.1 ENTRAPMENT IN POLYMERIC NETWORKS 368
9.4.2 ENTRAPMENT IN IONOTROPIC GELS 369
9.4.2.1 PRINCIPLE 369
9.4.2.2 EXAMPLES 372
9.5 ADSORPTION 375
9.6 ADHESION 376
9.6.1 BASIC CONSIDERATIONS 377
9.6.2 APPLICATIONS 383
9.6.2.1 ADHERENT MAMMALIAN CELLS FOR BIOPHARMACEUTICALS PRODUCTION 383
9.6.2.2 ANAEROBIC WASTEWATER TREATMENT 384
9.6.2.3 NITROGEN ELIMINATION (NITRIFICATION AND DENITRIFICATION) 391
9.6.2.4 EXHAUST GAS PURIFICATION 391
9.7 PERSPECTIVES 393
9.7.1 BIOFILM CATALYSIS 393
9.7.2 MICROBIAL FUEL CELLS 396
EXERCISES 399
REFERENCES 402
10 CHARACTERIZATION O
F
IMMOBILIZED BIOCATALYSTS 411
10.1 INTRODUCTION 412
10.2 FACTORS INFLUENCING THE SPACE-TIME YIELD OF IMMOBILIZED
BIOCATALYSTS 413
10.3 EFFECTIVENESS FACTORS FOR IMMOBILIZED BIOCATALYSTS 414
10.4 MASS TRANSFER AND REACTION 416
10.4.1 MAXIMAL REACTION RATE OF IMMOBILIZED BIOCATALYSTS AS A FUNCTION
OF
PARTICLE RADIUS 416
10.4.2 CALCULATION OF EFFECTIVENESS FACTORS AND CONCENTRATION PROFILES
INSIDE
AND OUTSIDE THE PARTICLES 418
10.5 SPACE-TIME YIELDS AND EFFECTIVENESS FACTORS FOR DIFFERENT
REACTORS 422
10.5.1 CONTINUOUS STIRRED TANK REACTOR 423
10.5.2 PACKED BED REACTOR OR STIRRED BATCH REACTOR 424
10.5.3 COMPARISON OF CSTAND PB REACTORS 425
10.6 DETERMINATION O
F
ESSENTIAL PROPERTIES OF IMMOBILIZED
BIOCATALYSTS 425
10.6.1 PHYSICOCHEMICAL PROPERTIES 428
10.6.1.1 IMMOBILIZED BIOCATALYST DISTRIBUTION AND CONFORMATION 429
10.6.1.2 STATIONARY CHARGE DENSITY IN THE SUPPORT 429
10.6.2 KINETIC CHARACTERIZATION O
F
IMMOBILIZED BIOCATALYSTS: INFLUENCE OF
SUPPORT PROPERTIES ON THE NANO- AND MICROMETER LEVEL IN AQUEOUS AND
OTHER SYSTEMS 430
10.6.2.1 DETERMINATION OF V'
MAX
, K'
CAT
, SUBSTRATE/PRODUCT CONCENTRATION,
AND P
H
GRADIENTS 431
CONTENTS | X
I
I
I
10.6.2.2 K'
M
AND K[ 434
10.6.2.3 SELECTIVITIES 435
10.6.2.4 DETERMINATIONS O
F
EFFECTIVENESS FACTORS 436
10.6.3 PRODUCTIVITY AND STABILITY UNDER PROCESS CONDITIONS 436
10.7 COMPARISON OF CALCULATED AND EXPERIMENTAL DATA FOR IMMOBILIZED
BIOCATALYSTS 437
10.8 APPLICATION O
F
IMMOBILIZED BIOCATALYSTS FOR ENZYME PROCESSES
IN AQUEOUS SUSPENSIONS 439
10.9 IMPROVING THE PERFORMANCE OF IMMOBILIZED BIOCATALYSTS 441
EXERCISES 443
REFERENCES 445
11 REACTORS AND PROCESS TECHNOLOGY 449
11.1 GENERAL ASPECTS, BIOCHEMICAL ENGINEERING, AND PROCESS
SUSTAINABILITY 449
11.1.1 BIOCHEMICAL ENGINEERING ASPECTS 450
11.1.2 PROCESS SUSTAINABILITY AND ECOLOGICAL CONSIDERATIONS 452
11.2 TYPES O
F
REACTORS 454
11.2.1 BASIC TYPES AND MASS BALANCES 455
11.2.2 OTHER REACTOR TYPES AND CONFIGURATIONS: APPLICATION EXAMPLES 460
11.3 RESIDENCE TIME DISTRIBUTION, MIXING, PRESSURE DROP, AND MASS
TRANSFER IN REACTORS 466
11.3.1 SCALE-UP, DIMENSIONLESS NUMBERS 466
11.3.2 RESIDENCE TIME DISTRIBUTION 468
11.3.3 MIXING IN STIRRED TANK REACTORS 471
11.3.4 MASS TRANSFER IN REACTORS 476
11.3.5 PRESSURE DROP AND FLUIDIZATION IN TUBULAR REACTORS 477
11.4 PROCESS TECHNOLOGY 478
11.4.1 SURVEY 478
11.4.2 PROCESS INTEGRATION 479
11.4.3 REACTOR INSTRUMENTATION 486
EXERCISES 486
LITERATURE 488
REFERENCES 488
12 CASE STUDIES 493
12.1 STARCH PROCESSING AND GLUCOSE ISOMERIZATION 493
12.1.1 STARCH PROCESSING 493
12.1.2 THE MANUFACTURE OF GLUCOSE-FRUCTOSE SYRUP 497
12.2 BIOFUELS FROM BIOMASS 501
12.2.1 STARCH-BASED ETHANOL PRODUCTION 502
12.2.2 LIGNOCELLULOSE-BASED BIOFUELS 507
12.2.2.1 GENERAL 507
12.2.2.2 RAW MATERIALS 508
12.2.2.3 PRETREATMENT 509
X
I
V
| CONTENTS
12.2.2.4 ENZYMES 512
12.2.2.5 PROCESSING AND REACTION ENGINEERING 517
12.2.2.6 PILOT STUDIES 519
12.2.2.7 ALTERNATIVE BIOCATALYST-BASED BIOFUELS 520
12.3 CASE STUDY: THE ONE-STEP ENZYMATIC PROCESS TO PRODUCE 7-ACA
FROM CEPHALOSPORIN C 521
12.3.1 ENZYME PROCESSES FOR THE PRODUCTION O
F
P-LACTAM ANTIBIOTICS 521
12.3.2 OVERALL PROCESS FOR THE PRODUCTION O
F
7-ACA 531
12.3.3 CONVERSION OF CEPHALOSPORIN C TO 7-ACA 533
12.3.4 REACTION CHARACTERIZATION AND IDENTIFICATION O
F
CONSTRAINSTS: HYDROLYSIS
O
F
CEPHALOSPORIN C 533
12.3.5 ENZYME CHARACTERIZATION AND IDENTIFICATION O
F
CONSTRAINTS:
CEPHALOSPORIN ACYLASE (OR GLUTARYL ACYLASE OR AMIDASE) 535
12.3.6 EVALUATION O
F
PROCESS OPTIONS 536
12.3.6.1 PROCESS WINDOW 536
12.3.6.2 SUITABLE REACTORS AND PH-CONTROLLING BUFFERS 537
12.3.6.3 REACTION END POINT AND IMMOBILIZED ENZYME REQUIREMENT FOR
MINIMUM SPACE-TIME YIELD 539
12.3.6.4 PRODUCT ISOLATION 539
12.4 CASE STUDY. BIOCATALYTIC PROCESS FOR THE SYNTHESIS OF THE LIPITOR
SIDE CHAIN 540
EXERCISES 543
REFERENCES 544
APPENDIX A: THE WORLD OF BIOTECHNOLOGY INFORMATION: SEVEN POINTS FOR
REFLECTING ON
YOUR INFORMATION BEHAVIOR 553
APPENDIX B: SOLUTIONS TO EXERCISES 565
APPENDIX C: SYMBOLS AND ABREVIATIONS 585
INDEX 591 |
| any_adam_object | 1 |
| author | Buchholz, Klaus 1941- Kasche, Volker 1939-2017 Bornscheuer, Uwe Theo 1964- |
| author_GND | (DE-588)118071696 (DE-588)11807170X (DE-588)121061841 |
| author_facet | Buchholz, Klaus 1941- Kasche, Volker 1939-2017 Bornscheuer, Uwe Theo 1964- |
| author_role | aut aut aut |
| author_sort | Buchholz, Klaus 1941- |
| author_variant | k b kb v k vk u t b ut utb |
| building | Verbundindex |
| bvnumber | BV040278359 |
| classification_rvk | VK 8700 VN 8900 WF 9720 WF 9730 |
| classification_tum | CHE 825f CIT 970f |
| ctrlnum | (OCoLC)802476866 (DE-599)DNB1021678481 |
| dewey-full | 660.634 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.634 |
| dewey-search | 660.634 |
| dewey-sort | 3660.634 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie Biologie Chemie Chemie-Ingenieurwesen Biotechnologie |
| edition | 2., completely rev., and enl. ed. |
| format | Book |
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| genre | 1\p (DE-588)4151278-9 Einführung gnd-content 2\p (DE-588)4123623-3 Lehrbuch gnd-content |
| genre_facet | Einführung Lehrbuch |
| id | DE-604.BV040278359 |
| illustrated | Illustrated |
| indexdate | 2025-03-10T13:01:37Z |
| institution | BVB |
| isbn | 9783527329892 9783527632916 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025133774 |
| oclc_num | 802476866 |
| open_access_boolean | |
| owner | DE-703 DE-M49 DE-BY-TUM DE-634 DE-11 DE-29T DE-188 DE-83 DE-1102 DE-19 DE-BY-UBM |
| owner_facet | DE-703 DE-M49 DE-BY-TUM DE-634 DE-11 DE-29T DE-188 DE-83 DE-1102 DE-19 DE-BY-UBM |
| physical | XX, 606 S. Ill., graph. Darst. 240 mm x 170 mm |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Wiley-Blackwell |
| record_format | marc |
| spelling | Buchholz, Klaus 1941- Verfasser (DE-588)118071696 aut Biocatalysts and enzyme technology Klaus Buchholz, Volker Kasche, and Uwe T. Bornscheuer 2., completely rev., and enl. ed. Weinheim Wiley-Blackwell 2012 XX, 606 S. Ill., graph. Darst. 240 mm x 170 mm txt rdacontent n rdamedia nc rdacarrier Enzymkatalyse (DE-588)4152480-9 gnd rswk-swf Biokatalyse (DE-588)4393622-2 gnd rswk-swf Enzymtechnologie (DE-588)4135962-8 gnd rswk-swf 1\p (DE-588)4151278-9 Einführung gnd-content 2\p (DE-588)4123623-3 Lehrbuch gnd-content Enzymtechnologie (DE-588)4135962-8 s DE-604 Enzymkatalyse (DE-588)4152480-9 s Biokatalyse (DE-588)4393622-2 s 3\p DE-604 Kasche, Volker 1939-2017 Verfasser (DE-588)11807170X aut Bornscheuer, Uwe Theo 1964- Verfasser (DE-588)121061841 aut Erscheint auch als Online-Ausgabe, PDF 978-3-527-63292-3 X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=4014757&prov=M&dok_var=1&dok_ext=htm Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025133774&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 3\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Buchholz, Klaus 1941- Kasche, Volker 1939-2017 Bornscheuer, Uwe Theo 1964- Biocatalysts and enzyme technology Enzymkatalyse (DE-588)4152480-9 gnd Biokatalyse (DE-588)4393622-2 gnd Enzymtechnologie (DE-588)4135962-8 gnd |
| subject_GND | (DE-588)4152480-9 (DE-588)4393622-2 (DE-588)4135962-8 (DE-588)4151278-9 (DE-588)4123623-3 |
| title | Biocatalysts and enzyme technology |
| title_auth | Biocatalysts and enzyme technology |
| title_exact_search | Biocatalysts and enzyme technology |
| title_full | Biocatalysts and enzyme technology Klaus Buchholz, Volker Kasche, and Uwe T. Bornscheuer |
| title_fullStr | Biocatalysts and enzyme technology Klaus Buchholz, Volker Kasche, and Uwe T. Bornscheuer |
| title_full_unstemmed | Biocatalysts and enzyme technology Klaus Buchholz, Volker Kasche, and Uwe T. Bornscheuer |
| title_short | Biocatalysts and enzyme technology |
| title_sort | biocatalysts and enzyme technology |
| topic | Enzymkatalyse (DE-588)4152480-9 gnd Biokatalyse (DE-588)4393622-2 gnd Enzymtechnologie (DE-588)4135962-8 gnd |
| topic_facet | Enzymkatalyse Biokatalyse Enzymtechnologie Einführung Lehrbuch |
| url | http://deposit.dnb.de/cgi-bin/dokserv?id=4014757&prov=M&dok_var=1&dok_ext=htm http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025133774&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT buchholzklaus biocatalystsandenzymetechnology AT kaschevolker biocatalystsandenzymetechnology AT bornscheueruwetheo biocatalystsandenzymetechnology |