Förster resonance energy transfer (FRET)-based detection of molecular binding interactions: from fundamental research to application in life science
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 129 Bl. graph. Darst. |
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Record in the Search Index
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| adam_text | IMAGE 1
CONTENTS
SUMMARY 1
ZUSAMMENFASSUNG 3
1 GENERAL INTRODUCTION 6
1.1 THE PHOTOPHYSICAL PHENOMENON FLUORESCENCE AND APPLICATIONS O F
FLUORESCENCE 7 SPECTROSCOPY
1.2 FORSTER RESONANCE ENERGY TRANSFER (FRET) - THE MOST POPULAR
FLUORESCENCE 8
PHENOMENON IN LIFE SCIENCE 1.3 REQUIREMENTS FOR A SUCCESSFUL APPLICATION
O F FLUORESCENCE PROBES IN LIFE SCIENCE 12
1.4 ADAPTATION O F FLUORESCENCE TECHNIQUES TO THE USERS DEMANDS 15
1.5 MOTIVATION O F MY THESIS 16
2 OXAZINE DYE-CONJUGATED DNA OLIGONUCLEOTIDES: FORSTER RESONANCE ENERGY
19 TRANSFER IN VIEW O F MOLECULAR DYE-DNA INTERACTIONS
2.1 ABSTRACT 20
2.2 INTRODUCTION 21
2.3 EXPERIMENTAL PROCEDURES 23
2.3.1 REAGENTS AND CHEMICALS 23
2.3.2 HYBRIDIZATION O F THE DYE-SSDNA CONJUGATES 24
2.3.3 ABSORPTION AND FLUORESCENCE SPECTROSCOPY 25
2.3.4 FLUORESCENCE ANISOTROPY 26
2.3.5 FLUORESCENCE CORRELATION SPECTROSCOPY (FCS) 27
2.3.6 FORSTER RESONANCE ENERGY TRANSFER (FRET) 28
2.4 RESULTS 30
2.4.1 EFFECTS O F THE BIOCONJUGATION ON THE BASIC PHOTOPHYSICAL
PROPERTIES O F THE DYES .... 30 2.4.2 INTERACTION BETWEEN CONJUGATED
DYES AND SSDNA 33
2.4.3 INTERACTION BETWEEN CONJUGATED DYES AND DSDNA 35
2.4.4 FORSTER RESONANCE ENERGY TRANSFER (FRET) 37
2.5 DISCUSSION 40
2.6 REFERENCES 46
2.7 SUPPORTING INFORMATION 52
3 NOVEL INTRAMOLECULAR ENERGY TRANSFER PROBE FOR THE DETECTION O F
BENZO[A]PYRENE 56 METABOLITES IN A HOMOGENEOUS COMPETITIVE FLUORESCENCE
IMMUNOASSAY
3.1 ABSTRACT 57
3.2 INTRODUCTION 57
3.3 EXPERIMENTAL METHODS 59
3.3.1 REAGENTS AND CHEMICALS 59
HTTP://D-NB.INFO/1025956451
IMAGE 2
5.1 PHOTOPHYSICAL CHARACTERIZATION O F PROMISING REPORTER DYES FOR
FRET-BASED LIFE 98
SCIENCE APPLICATIONS
5.2 IN-DEPTH CHARACTERIZATION O F MOLECULAR INTERACTIONS BETWEEN
REPORTER DYES AND 100
BIOMOLECULES SUCH AS DNA AND ANTIBODIES
5.3 INVESTIGATION O F A NOVEL FRET-BASED IMMUNODETECTION SCHEME FOR
LOW-MOLECULAR- 104 WEIGHT ANALYTES
5.4 ADAPTATION O F A WELL-ESTABLISHED AND CLINICALLY APPROVED, HIGHLY
SENSITIVE FRET- 105 BASED TR-FIA TECHNOLOGY TO POCT REQUIREMENTS
5.5 OUTLOOK 106
6 REFERENCES FOR GENERAL INTRODUCTION AND GENERAL DISCUSSION 110
7 APPENDIX 125
8 ACKNOWLEDGEMENTS 127
9 AUTHORS CONTRIBUTION STATEMENT 129
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| spelling | Kupstat, Annette Verfasser aut Förster resonance energy transfer (FRET)-based detection of molecular binding interactions from fundamental research to application in life science vorgelegt von Annette Kupstat 2012 129 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Potsdam, Univ., Diss., 2012 Fluoreszenzfarbstoff (DE-588)4154826-7 gnd rswk-swf Fotophysik (DE-588)4193388-6 gnd rswk-swf Fluoreszenz-Resonanz-Energie-Transfer (DE-588)4773018-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Fluoreszenz-Resonanz-Energie-Transfer (DE-588)4773018-3 s Fotophysik (DE-588)4193388-6 s Fluoreszenzfarbstoff (DE-588)4154826-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025123285&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kupstat, Annette Förster resonance energy transfer (FRET)-based detection of molecular binding interactions from fundamental research to application in life science Fluoreszenzfarbstoff (DE-588)4154826-7 gnd Fotophysik (DE-588)4193388-6 gnd Fluoreszenz-Resonanz-Energie-Transfer (DE-588)4773018-3 gnd |
| subject_GND | (DE-588)4154826-7 (DE-588)4193388-6 (DE-588)4773018-3 (DE-588)4113937-9 |
| title | Förster resonance energy transfer (FRET)-based detection of molecular binding interactions from fundamental research to application in life science |
| title_auth | Förster resonance energy transfer (FRET)-based detection of molecular binding interactions from fundamental research to application in life science |
| title_exact_search | Förster resonance energy transfer (FRET)-based detection of molecular binding interactions from fundamental research to application in life science |
| title_full | Förster resonance energy transfer (FRET)-based detection of molecular binding interactions from fundamental research to application in life science vorgelegt von Annette Kupstat |
| title_fullStr | Förster resonance energy transfer (FRET)-based detection of molecular binding interactions from fundamental research to application in life science vorgelegt von Annette Kupstat |
| title_full_unstemmed | Förster resonance energy transfer (FRET)-based detection of molecular binding interactions from fundamental research to application in life science vorgelegt von Annette Kupstat |
| title_short | Förster resonance energy transfer (FRET)-based detection of molecular binding interactions |
| title_sort | forster resonance energy transfer fret based detection of molecular binding interactions from fundamental research to application in life science |
| title_sub | from fundamental research to application in life science |
| topic | Fluoreszenzfarbstoff (DE-588)4154826-7 gnd Fotophysik (DE-588)4193388-6 gnd Fluoreszenz-Resonanz-Energie-Transfer (DE-588)4773018-3 gnd |
| topic_facet | Fluoreszenzfarbstoff Fotophysik Fluoreszenz-Resonanz-Energie-Transfer Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025123285&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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