Single-stranded DNA binding proteins: methods and protocols
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2012
|
| Schriftenreihe: | Methods in molecular biology
922 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 259 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Single-stranded DNA binding proteins |b methods and protocols |c ed. by James L. Keck |
| 264 | 1 | |a New York [u.a.] |b Humana Press |c 2012 | |
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Datensatz im Suchindex
| _version_ | 1804149246001152000 |
|---|---|
| adam_text | Single-Stranded DNA Binding
Proteins
Methods and Protocols
Edited by
James L Keck
Department of Biomolecular Chemistry, School of Medicine and Public Health,
University of Wisconsin, Madison, Wl, USA
Humana Press
Contents
Preface v
Contributors ix
1 Functions of Single-Strand DNA-Binding Proteins in DNA Replication,
Recombination, and Repair 1
Aimee H Marceau
2 Structural Diversity Based on Variability in Quaternary Association
A Case Study Involving Eubacterial and Related SSBs 23
S M Arif and M Vijayan
3 SSB Binding to ssDNA Using Isothermal Titration Calorimetry 37
Alexander G Kozlov and Timothy M Lohman
4 SSB-DNA Binding Monitored by Fluorescence Intensity and Anisotropy 55
Alexander G Kozlov, Roberto Galletto, and Timothy M Lohman
5 Single-Molecule Analysis of SSB Dynamics on Single-Stranded DNA 85
Ruobo Zhou and Taekjip Ha
6 Sample Preparation Methods to Analyze DNA- Induced Structural
Changes in Replication Protein A 101
Chris A Brosey, Susan E Tsutakawa, and Walter J Chazin
7 Structural Studies of SSB Interaction with RecC) 123
Mikhail Ryzhikov and Sergey Korolev
8 Investigation of Protein-Protein Interactions of Single-Stranded
DNA-Binding Proteins by Analytical Ultracentrifugation 133
Natalie Naue and Ute Curth
9 Ammonium Sulfate Co-precipitation of SSB and Interacting Proteins 151
Aimee H Marceau
10 Analyzing Interactions Between SSB and Proteins by the Use
of Fluorescence Anisotropy 155
Duo Lu
11 Far Western Blotting as a Rapid and Efficient Method for Detecting
Interactions Between DNA Replication and DNA Repair Proteins 161
Brian W Walsh, Justin S Lenhart, Jeremy W Schroeder, and Lyle A Simmons
12 Methods for Analysis of SSB-Protein Interactions by SPR 169
Asher N Page and Nicholas P George
13 Use of Native Gels to Measure Protein Binding to SSB 175
Jin Inoue and Tsutomu Mikawa
vii
viii Contents
14 Identification of Small Molecules That Disrupt SSB-Protein Interactions
Using a High-Throughput Screen 183
Douglas A Bernstein
15 Detection of Posttranslational Modifications of Replication Protein A 193
Cathy S Hass, Ran Chen, and Marc S Wold
16 Detecting Posttranslational Modifications of Bacterial SSB Proteins 205
Dusica Vujaklija and Boris Macek
17 Fluorescent SSB as a Reagentless Biosensor for Single-Stranded DNA 219
Katy Hedgethorne and Martin R Webb
18 Fluorescent Single-Stranded DNA-Binding Proteins Enable In Vitro
and In Vivo Studies 235
Piero R Bianco, Adam J Stanenas, Juan Liu, and Christopher S Cohan
19 Use of Fluorescently Tagged SSB Proteins in In Vivo Localization
Experiments 245
Rodrigo Reyes-Lamothe
Index 255
|
| any_adam_object | 1 |
| author | Keck, James L. |
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| dewey-full | 572.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.6 |
| dewey-search | 572.6 |
| dewey-sort | 3572.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| id | DE-604.BV040250016 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:19:58Z |
| institution | BVB |
| language | English |
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| physical | X, 259 S. Ill., graph. Darst. |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Keck, James L. Verfasser aut Single-stranded DNA binding proteins methods and protocols ed. by James L. Keck New York [u.a.] Humana Press 2012 X, 259 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 922 Springer protocols Methods in molecular biology 922 (DE-604)BV035362695 922 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025106013&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Keck, James L. Single-stranded DNA binding proteins methods and protocols Methods in molecular biology |
| title | Single-stranded DNA binding proteins methods and protocols |
| title_auth | Single-stranded DNA binding proteins methods and protocols |
| title_exact_search | Single-stranded DNA binding proteins methods and protocols |
| title_full | Single-stranded DNA binding proteins methods and protocols ed. by James L. Keck |
| title_fullStr | Single-stranded DNA binding proteins methods and protocols ed. by James L. Keck |
| title_full_unstemmed | Single-stranded DNA binding proteins methods and protocols ed. by James L. Keck |
| title_short | Single-stranded DNA binding proteins |
| title_sort | single stranded dna binding proteins methods and protocols |
| title_sub | methods and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025106013&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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