Enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the Cladoceran Daphnia magna:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Berlin
Logos Verl.
2012
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. Sprache |
| Physical Description: | IX, 109 S. Ill., graph. Darst. |
| ISBN: | 9783832531454 |
Staff View
MARC
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| 100 | 1 | |a Ortiz Rodríguez, Rafael |d 1977- |e Verfasser |0 (DE-588)1022074989 |4 aut | |
| 245 | 1 | 0 | |a Enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the Cladoceran Daphnia magna |c von Rafael Ortiz Rodrĺguez |
| 264 | 1 | |a Berlin |b Logos Verl. |c 2012 | |
| 300 | |a IX, 109 S. |b Ill., graph. Darst. | ||
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| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zsfassung in dt. Sprache | ||
| 502 | |a Zugl.: Berlin, Humboldt-Univ., Diss., 2012 | ||
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Record in the Search Index
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|---|---|
| adam_text | IMAGE 1
TABLE O F CONTENTS
SUMMARY I ZUSAMMENFASSUNG I
PUBLICATIONS AND CONFERENCE PARTICIPATION VII
LIST O F ABBREVIATIONS VIII
1. INTRODUCTION
I. I CYANOBACTERIA 1
I . I . I CYANOBACTERIA IN THE LIMNIC ECOSYSTEM 1
1. 1.2 CYANOBACTERIAL BLOOMS 2
1. 1.3 CYANOBACTERIAL TOXINS AND MICROCYSTIN 3
1.1.3.1 MICROCYSTIN TOXICITY 5
1. 1.3.2 OXIDATIVE STRESS 5
1.1.3.3 COPING WITH MICROCYSTIN 8
1. 1.3.4 THE PHYSIOLOGICAL COSTS O F COPING WITH MICROCYSTIN 1 1
1.2 THE CLADOCERAN DAPHNIA MAGNA 12
1.2.1 THE BIOLOGY O F DAPHNIA 12
1.2.2 ECOLOGICAL FUNCTION O F DAPHNIA 13
1.2.3 DAPHNIA AS A MODEL ORGANISM IN ECOLOGY AND TOXICOLOGY 14
1.2.4 SINGLE AND MULTIGENERATIONAL TOXICITY TESTS WITH DAPHNIA SP. 15
1.2.5 TRANS- O R MULTIGENERATIONAL EFFECTS ON DAPHNIA MAGNA 16
1.3 THE INTERACTION BETWEEN TOXIC CYANOBACTERIA AND DAPHNIA MAGNA 18
1.4 RATIONALE F O R THE INVESTIGATION 20
1.5 AIMS O F THE STUDY 21
2. MATERIALS AND METHODS 23
2.1 MATERIALS 23
2.1.1 LIST O F EQUIPMENT 23
2.1.2 LIST O F CHEMICALS 24
HTTP://D-NB.INFO/1022024507
IMAGE 2
TABLE OF CONTENTS
2.1.3 THE TOXIN MICROCYSTIN-LR 26
2.1.4 THE EXPERIMENTAL ORGANISM DAPHNIA MAGNA 26
2.1.5 GREEN ALGAE CULTIVATION 27
2.2 METHODS 27
2.2.1 ENZYME EXTRACTION 27
2.2.2 ENZYME ACTIVITY MEASUREMENT 28
2.2.2.1 GLUTATHIONE S TRANSFERASE 28
2.2.2.2 CATALASE 28
2.2.2.3 LACTATE DEHYDROGENASE 29
2.2.2.4 MALATE DEHYDROGENASE 29
2.2.3 PROTEIN QUANTIFICATION 30
2.2.4 LIPID PEROXIDATION 30
2.2.5 LACTATE 30
2.2.6 WEIGHT 31
2.3 EXPERIMENTAL SETUP 3 1
2.3.1 DETERMINATION O F THE ACUTE EFFECTS O F MC-LR ON
BIOTRANSFORMATION,
ANTIOXIDANT AND ENERGY METABOLISM ENZYMES AND ON LIPID PEROXIDATION 31
AND LACTATE O F DAPHNIA MAGNA AT DIFFERENT AGES
2.3.2 DETERMINATION O F THE ACUTE EFFECTS O F MC-LR ON
BIOTRANSFORMATION,
ANTIOXIDANT AND ENERGY METABOLISM ENZYME ACTIVITIES O F DAPHNIA MAGNA S
32
PROGENY ORIGINATED F R O M PREVIOUSLY ACUTE O R SUBCHRONIC EXPOSED
MOTHERS T O DIFFERENT MC-LR CONCENTRATIONS
2.3.3 DETERMINATION O F THE CHRONIC EFFECTS O F MC-LR ON THE SURVIVAL
AND
GROWTH O F DAPHNIA MAGNA S PROGENY ORIGINATED FROM PREVIOUSLY ACUTE O R
3 5
SUBCHRONIC EXPOSED MOTHERS T O DIFFERENT MC-LR CONCENTRATIONS
2.4 STATISTICAL ANALYSIS 37
IMAGE 3
TABLE O F CONTENTS
3. RESULTS
3.1 DETERMINATION O F THE ACUTE EFFECTS O F MC-LR ON BIOTRANSFORMATION,
ANTIOXIDANT AND ENERGY METABOLISM ENZYMES AND ON LIPID PEROXIDATION AND
3 9
LACTATE O F DAPHNIA MAGNA AT DIFFERENT AGES
3.1.1 SOLUBLE GLUTATHIONE S TRANSFERASE 3 9
3.1.2 MICROSOMAL GLUTATHIONE S TRANSFERASE 40
3.1.3 CATALASE 4 1
3.1.4 LACTATE DEHYDROGENASE 4 2
3.1.2 THIOBARBITURIC ACID REACTIVE SUBSTANCES 4 3
3.1.3 LACTATE 4 4
3.2 DETERMINATION O F THE ACUTE EFFECTS O F MC-LR ON BIOTRANSFORMATION,
ANTIOXIDANT AND ENERGY METABOLISM ENZYME ACTIVITIES O F DAPHNIA MAGNA S
45
PROGENY ORIGINATED FROM PREVIOUSLY ACUTE O R SUBCHRONIC EXPOSED MOTHERS
T O
DIFFERENT MC-LR CONCENTRATIONS
3.2.1 CODES 4 5
3.2.2 SOLUBLE GLUTATHIONE S TRANSFERASE 4 6
3.2.3 MICROSOMAL GLUTATHIONE S TRANSFERASE 4 7
3.2.4 CATALASE 4 G
3.2.5 LACTATE DEHYDROGENASE 4 9
3.2.6 MALATE DEHYDROGENASE 5 0
3.3 DETERMINATION O F THE CHRONIC EFFECTS O F MC-LR ON THE SURVIVAL AND
GROWTH OF
DAPHNIA MAGNA S PROGENY ORIGINATED FROM PREVIOUSLY ACUTE O R SUBCHRONIC
5 1
EXPOSED MOTHERS T O DIFFERENT MC-LR CONCENTRATIONS.
3.3.1 SURVIVAL 5 1
3.3.2 WEIGHT 5 3
IMAGE 4
TABLE O F CONTENTS
4. DISCUSSION
4.1 THE ACUTE EFFECTS O F MC-LR ON BIOTRANSFORMATION, ANTIOXIDANT AND
ENERGY
METABOLISM ENZYMES AND ON LIPID PEROXIDATION AND LACTATE O F DAPHNIA
MAGNA
AT DIFFERENT AGES
4.2 THE ACUTE EFFECTS O F MC-LR ON BIOTRANSFORMATION, ANTIOXIDANT AND
ENERGY
METABOLISM ENZYME ACTIVITIES O F DAPHNIA MAGNA S PROGENY ORIGINATED F R
O M
PREVIOUSLY ACUTE O R SUBCHRONIC EXPOSED MOTHERS T O DIFFERENT MC-LR
CONCENTRATIONS
4.2.1 EXPERIMENTAL DESIGN
4.2.2 BIOTRANSFORMATION AND ANTIOXIDANT ENZYMES
4.2.3 ENERGY METABOLISM ENZYMES
4.2.4 CONSIDERING ALL ENZYMES (BIOTRANSFORMATION, ANTIOXIDANT AND ENERGY
METABOLISM)
4.3 DETERMINATION O F THE CHRONIC EFFECTS O F MC-LR ON THE SURVIVAL AND
GROWTH OF
DAPHNIA MAGNA S PROGENY ORIGINATED FROM PREVIOUSLY ACUTE O R SUBCHRONIC
EXPOSED MOTHERS T O DIFFERENT MC-LR CONCENTRATIONS
4.3.1 SURVIVAL
4.3.2 WEIGHT
4.4 CONSIDERING ALL VARIABLES
5. CONCLUSIONS AND PERSPECTIVES
5.1 CONCLUSIONS
5.2 PERSPECTIVES
6. APPENDIX
6.1 THE ACUTE EFFECTS O F MC-LR ON TOTAL CARBOHYDRATES AND GLYCOGEN O F
DAPHNIA
MAGNA NEONATES AND ADULTS
6.2 THE EFFECTS O F MC-LR ON DAPHNIA MAGNA S GROWTH AFTER 7 DAYS O F
EXPOSURE
6.3 EFFECTS O F CYANOBACTERIAL CRUDE EXTRACTS ON CARBOHYDRATES, GLYCOGEN
AND
MALATE DEHYDROGENASE O F DAPHNIA MAGNA
55
59
59
60
63
63
65
65
68
69
73
74
77
79
81
IMAGE 5
7. REFERENCES
SELBSTANDIGKEITSERKLARUNG
CURRICULUM VITAE
TABLE O F CONTENTS
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| author | Ortiz Rodríguez, Rafael 1977- |
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| author_facet | Ortiz Rodríguez, Rafael 1977- |
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| building | Verbundindex |
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| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.9571532 |
| dewey-search | 571.9571532 |
| dewey-sort | 3571.9571532 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| indexdate | 2024-07-10T00:17:19Z |
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| isbn | 9783832531454 |
| language | English |
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| spelling | Ortiz Rodríguez, Rafael 1977- Verfasser (DE-588)1022074989 aut Enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the Cladoceran Daphnia magna von Rafael Ortiz Rodrĺguez Berlin Logos Verl. 2012 IX, 109 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache Zugl.: Berlin, Humboldt-Univ., Diss., 2012 Glutathiontransferasen (DE-588)4314134-1 gnd rswk-swf Großer Wasserfloh (DE-588)4158265-2 gnd rswk-swf Microcystine (DE-588)4429944-8 gnd rswk-swf Vererbung (DE-588)4187668-4 gnd rswk-swf Toleranz Biologie (DE-588)4308830-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Großer Wasserfloh (DE-588)4158265-2 s Microcystine (DE-588)4429944-8 s Toleranz Biologie (DE-588)4308830-2 s Vererbung (DE-588)4187668-4 s Glutathiontransferasen (DE-588)4314134-1 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024975833&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ortiz Rodríguez, Rafael 1977- Enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the Cladoceran Daphnia magna Glutathiontransferasen (DE-588)4314134-1 gnd Großer Wasserfloh (DE-588)4158265-2 gnd Microcystine (DE-588)4429944-8 gnd Vererbung (DE-588)4187668-4 gnd Toleranz Biologie (DE-588)4308830-2 gnd |
| subject_GND | (DE-588)4314134-1 (DE-588)4158265-2 (DE-588)4429944-8 (DE-588)4187668-4 (DE-588)4308830-2 (DE-588)4113937-9 |
| title | Enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the Cladoceran Daphnia magna |
| title_auth | Enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the Cladoceran Daphnia magna |
| title_exact_search | Enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the Cladoceran Daphnia magna |
| title_full | Enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the Cladoceran Daphnia magna von Rafael Ortiz Rodrĺguez |
| title_fullStr | Enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the Cladoceran Daphnia magna von Rafael Ortiz Rodrĺguez |
| title_full_unstemmed | Enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the Cladoceran Daphnia magna von Rafael Ortiz Rodrĺguez |
| title_short | Enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the Cladoceran Daphnia magna |
| title_sort | enzymatic mechanisms of cyanobacterial toxin tolerance transfer to offspring in the cladoceran daphnia magna |
| topic | Glutathiontransferasen (DE-588)4314134-1 gnd Großer Wasserfloh (DE-588)4158265-2 gnd Microcystine (DE-588)4429944-8 gnd Vererbung (DE-588)4187668-4 gnd Toleranz Biologie (DE-588)4308830-2 gnd |
| topic_facet | Glutathiontransferasen Großer Wasserfloh Microcystine Vererbung Toleranz Biologie Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024975833&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ortizrodriguezrafael enzymaticmechanismsofcyanobacterialtoxintolerancetransfertooffspringinthecladocerandaphniamagna |