Difference gel electrophoresis (DIGE): methods and protocols
Literaturangaben
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
New York, NY
Humana Press
2012
|
| Series: | Methods in molecular biology
854 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Summary: | Literaturangaben |
| Physical Description: | XIII, 401 S. Ill., graph. Darst. |
| ISBN: | 1617795720 9781617795725 |
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Record in the Search Index
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| adam_text | Titel: Difference gel electrophoresis (DIGE)
Autor: Cramer, Rainer
Jahr: 2012
Contents
Preface.......................................................... v
Contributors...................................................... xi
Part I Fundamentals
1 DIGE: Past and Future.......................................... 3
Jonathan S. Minden
2 The Basics of 2D DIGE......................................... 9
Phil Beckett
3 Multifluorescence 2D Gel Imaging and Image Analysis.................. 21
Ingo Vormbrock, Sonja Hartwig, and Stefan Lehr
4 Assessing Signal-to-Noise in Quantitative Proteomics:
Multivariate Statistical Analysis in DIGE Experiments................... 31
David B. Friedman
5 Analysis of Proteins Using DIGE and MALDI Mass Spectrometry......... 47
Witold M. Winnik, Robert M. DeKroon, Joseph S.T. Jeong,
Mihaela Mocanu, Jennifer B. Robinette, Cristina Osorio,
Nedyalka N. Dicheva, Eric Hamlett, and Oscar Alzate
6 Synthesis and Validation of Cyanine-Based Dyes for DIGE............... 67
Michael E. Jung, Wan-Joong Kim, Nuraly K. Avliyakulov,
Merve Oztug, and Michael J. Haykinson
Part II Methods
7 2D DIGE Saturation Labeling for Minute Sample Amounts.............. 89
Georg J. Arnold and Thomas Fröhlich
8 Proteomic Analysis of Redox-Dependent Changes Using
Cysteine-Labeling 2D DIGE...................................... 113
Hong-Lin Chan, John Sinclair, and John F. Timms
9 Analysis of Protein Posttranslational Modifications Using
DIGE-Based Proteomics......................................... 129
Robert M. DeKroon, Jennifer B. Robinette, Cristina Osorio,
Joseph S.T. Jeong, Eric Hamlett, Mihaela Mocanu,
and Oscar Alzate
10 Comparative Analyses of Protein Complexes by Blue Native DIGE......... 145
Katrin Peters and Hans-Peter Braun
11 2D DIGE Analysis of Protein Extracts from Muscle Tissue............... 155
Cecilia Gelfi and Sara De Palma
VII
viii Contents
12 Combination of Highly Efficient Hexapeptide Ligand Library-Based
Sample Preparation with 2D DIGE for the Analysis of the Hidden
Human Serum/Plasma Proteome.................................. 169
Sonja Hartwig and Stefan Lehr
13 2D DIGE Analysis of Serum After Fractionation
by ProteoMiner™ Beads......................................... 181
Cynthia Liang, Gek San Tan, and Maxey CM. Chung
14 Study Design in DIGE-Based Biomarker Discovery..................... 195
Alexandra Graf and Rudolf Oehler
15 Comparative 2D DIGE Analysis of the Depleted Serum Proteome
for Biomarker Discovery......................................... 207
Megan Penno, Matthias Ernst, and Peter Hoffmann
Part III Applications in Clinical Proteomics
16 Differential Gel-Based Proteomic Approach for Cancer Biomarker
Discovery Using Human Plasma................................... 223
Keun Na, Min-Jung Lee, Hye-Jin Jeong, Hqguen Kim,
and Toung-Ki Paik
17 2D DIGE for the Analysis of RAMOS Ceils Subproteomes............... 239
Marisol Fernandez and Juan Pablo Albar
18 Application of Saturation Labeling in Lung Cancer Proteomics............ 253
Gereon Poschmann, Barbara Sitek, Bence Sipos, and Kai Stühler
19 Proteomic Profiling of the Epithelial-Mesenchymal Transition
Using 2D DIGE............................................... 269
Rommel A. Mathias, Hongji, and Richard J. Simpson
20 Method for Protein Subfractionation of Cardiovascular Tissues
Before DIGE Analysis........................................... 287
Athanasios Didangelos, Xiaoke Tin, and Manuel Mayr
21 Application of DIGE and Mass Spectrometry in the Study
of Type 2 Diabetes Mellitus Mouse Models........................... 299
Celia Smith, Davinia Mills, and Rainer Cramer
22 Evaluating the Efficacy of Subcellular Fractionation of Blast Cells
Using Live Cell Labeling and 2D DIGE............................. 319
Tin Ting Ho, Megan Penno, Michelle Perugini, Ian Lewis,
and Peter Hoffmann
Part IV Applications in Animal, Plant, and Microbial Proteomics
23 DIGE Analysis of Plant Tissue Proteomes Using a Phenolic
Protein Extraction Method....................................... 335
Christina Rode, Traud Winkelmann, Hans-Peter Braun,
and Frank Colditz
24 Native DIGE of Fluorescent Plant Protein Complexes................... 343
Veronika Reisinger and Lutz Andreas Eichacker
Contents ix
25 An Overview of 2D DIGE Analysis of Marine (Environmental) Bacteria..... 355
RalfRabus
26 Application of 2D DIGE in Animal Proteomics........................ 373
Ingrid Miller
Index........................................................... 397
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| language | English |
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| physical | XIII, 401 S. Ill., graph. Darst. |
| publishDate | 2012 |
| publishDateSearch | 2012 |
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| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Difference gel electrophoresis (DIGE) methods and protocols ed. by Rainer Cramer ... New York, NY Humana Press 2012 XIII, 401 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 854 Springer protocols Literaturangaben Gelelektrophorese (DE-588)4123267-7 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Gelelektrophorese (DE-588)4123267-7 s Labortechnik (DE-588)4123602-6 s b DE-604 Cramer, Rainer Sonstige oth Erscheint auch als Online-Ausgabe 978-1-617-79573-2 Methods in molecular biology 854 (DE-604)BV035362695 854 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024973571&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Difference gel electrophoresis (DIGE) methods and protocols Methods in molecular biology Gelelektrophorese (DE-588)4123267-7 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4123267-7 (DE-588)4123602-6 (DE-588)4143413-4 |
| title | Difference gel electrophoresis (DIGE) methods and protocols |
| title_auth | Difference gel electrophoresis (DIGE) methods and protocols |
| title_exact_search | Difference gel electrophoresis (DIGE) methods and protocols |
| title_full | Difference gel electrophoresis (DIGE) methods and protocols ed. by Rainer Cramer ... |
| title_fullStr | Difference gel electrophoresis (DIGE) methods and protocols ed. by Rainer Cramer ... |
| title_full_unstemmed | Difference gel electrophoresis (DIGE) methods and protocols ed. by Rainer Cramer ... |
| title_short | Difference gel electrophoresis (DIGE) |
| title_sort | difference gel electrophoresis dige methods and protocols |
| title_sub | methods and protocols |
| topic | Gelelektrophorese (DE-588)4123267-7 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Gelelektrophorese Labortechnik Aufsatzsammlung |
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