Molecular biology:
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2013
|
| Edition: | 2. ed. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XV, 907 S. Ill., graph. Darst. 28 cm |
| ISBN: | 9780123785947 0123785944 |
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MARC
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| 020 | |a 9780123785947 |c (pbk.) £48.99 |9 978-0-12-378594-7 | ||
| 020 | |a 0123785944 |c (pbk.) £48.99 |9 0-12-378594-4 | ||
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| 100 | 1 | |a Clark, David P. |d 1952- |e Verfasser |0 (DE-588)131505998 |4 aut | |
| 245 | 1 | 0 | |a Molecular biology |c David P. Clark ; Nanette J. Pazdernik |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2013 | |
| 300 | |a XV, 907 S. |b Ill., graph. Darst. |c 28 cm | ||
| 336 | |b txt |2 rdacontent | ||
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| 700 | 1 | |a Pazdernik, Nanette Jean |e Verfasser |0 (DE-588)136402100 |4 aut | |
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Record in the Search Index
| _version_ | 1804149059108208640 |
|---|---|
| adam_text | Contents
UNIT
1
Basic Chemical
and Biological Principles
1
CHAPTER I Cells and Organisms
_______2
1.
What Is Life?
3
2.
Living Creatures Are Made of Cells
4
3.
Eubacteria and Archaea Are Genetically
Distinct
8
4.
Eukaryotic Cells Are Subdivided into
Compartments
9
5.
The Diversity of Eukaryotes
13
6.
Haploidy, Diploidy, and the Eukaryote Cell Cycle
14
7.
Organisms Are Classified
15
8.
Some Widely-Studied Organisms Serve as Models
16
9.
Basic Characteristics of a Model Organism
10.
Purifying
DNA
from Model Organisms
11.
Viruses Are Not Living Cells
12.
Bacterial Viruses Infect Bacteria
13.
Human Viral Diseases Are Common
14.
A Variety of Subcellular Genetic Entities Exist
Key Concepts
Review Questions
Conceptual Questions
CHAPTER
2
Basic Genetics
27
27
29
30
32
32
34
35
36
37
1. Gregor
Mendel, The Father of Classical Genetics
37
2.
Genes Determine Each Step in Biochemical
Pathways
39
3.
Mutants Result from Alterations in Genes
40
4.
Phenotypes and Genotypes
41
5.
Chromosomes Are Long, Thin Molecules
That Carry Genes
42
6.
Dominant and Recessive
Alíeles
45
7.
Genes from Both Parents Are Mixed by Sexual
Reproduction
48
8.
Neighboring Genes Are Linked During
Inheritance Unless the
DNA
Recombines
52
9.
Identifying Genes that Cause Human Diseases
57
Key Concepts
58
Review Questions
59
Conceptual Questions
60
CHAPTER
3
DNA, RNA,
and Protein
62
1.
History of
DNA
as the Genetic Material
62
2.
Nucleic Acid Molecules Carry Genetic
Information
63
3.
Chemical Structure of Nucleic Acids
63
4.
Double-Stranded
DNA
Forms a Double Helix
67
5.
Constituents of Chromosomes
75
6.
The Central Dogma Outlines the Flow of
Genetic Information
78
7.
Ribosomes Read the Genetic Code
81
8.
Various Classes of
RNA
Have Different Functions
82
9.
Proteins Carry Out Many Cell Functions
84
Key Concepts
91
Review Questions
92
Conceptual Questions
93
CHAPTER
4
Genomes and
DNA 94
1.
Genome Organization
94
2.
Repeated Sequences Are a Feature of
Eukaryotic
DNA
100
3.
Palindromes, Inverted Repeats, and Stem and
Loop Structures
105
4.
Multiple
А
-Tracts Cause
DNA
to Bend
106
5.
Supercoiling Is Necessary for Packaging
of Bacterial
DNA
106
6.
Separation of
DNA
Fragments by
Electrophoresis
111
7.
Alternative Helical Structures of
DNA
Occur
113
8.
Packaging
DNA in
Eukaryotic Nuclei
116
Key Concepts
121
Review Questions
122
Conceptual Questions
123
CHAPTER
5
Manipulation of
Nucleic Acids
125
1.
Manipulating
DNA 126
2.
Chemical Synthesis of
DNA 135
3.
Measuring the Concentration of
DNA
and
RNA
with Ultraviolet Light
143
4.
Radioactive Labeling of Nucleic Acids
144
5.
Fluorescence in the Detection of
DNA
and
RNA
146
6.
The Electron Microscope
149
7.
Hybridization of
DNA
and
RNA
151
Key Concepts
158
Review Questions
158
Conceptual Questions
159
UNIT
2
The Genome
162
CHAPTER
6
Polymerase
Chain Reaction
163
1.
Fundamentals of the Polymerase Chain Reaction
164
2.
Inverse PCR
171
xii Contents
3.
Randomly Amplified Polymorphic
DNA
fRAPD)
172
I, Re
erse
Transcriptase PCR
174
X
Di
lïerential
Display PCR
175
>■·■„
Rapid Amplification of cDNA Ends (RACE)
176
,
PC
R
in Genetic Engineering
179
■S. Directed Mutagenesis
179
У.
Engineering Deletions and Insertions
by PCR
181
10.
Real-Time Fluorescent PCR
182
11.
Molecular Beacons and Scorpion Primers
184
12.
Use of PCR in Medical Diagnosis
188
13.
Environmental Analysis by PCR
189
14.
Rescuing
DNA
from Extinct Life Forms
by PCR
190
Key Concepts
191
Review Questions
192
Conceptual Questions
193
CHAPTER
7
Cloning Genes
for Analysis
194
1.
Properties of Cloning Vectors
195
2.
Detecting Insertions in Vectors
199
3.
Moving Genes Between Organisms:
Shuttle Vectors
201
4.
Bacteriophage Lambda Vectors
205
5.
Cosmid Vectors
206
6.
Yeast Artificial Chromosomes
208
7.
Bacterial and PI Artificial Chromosomes
208
8.
Recombineering Increases the Speed of
Gene Cloning
209
9.
A
DNA
Library is a Collection of Genes
from One Source
212
10.
Cloning Complementary
DNA
Avoids
Introns
215
11.
Chromosome Walking
217
12.
Cloning by Subtractive Hybridization
219
13.
Expression Vectors
220
Key Concepts
223
Review Questions
224
Conceptual Questions
225
CHAPTER
8 DNA
Sequencing
227
1. DNA
Sequencing—General Principles for
Chain Termination Sequencing
228
2.
Primer Walking Along a Strand of
DNA 234
3.
Automated Sequencing
235
4.
Cycle Sequencing
236
5.
The Emergence of
DNA
Chip Technology
237
6.
Pyrosequencing
239
7.
Second-Generation Sequencing
239
8.
Third-Generation Sequencing
242
9.
Nanopore Detectors for
DNA 243
Key Concepts
244
Review Questions
246
Conceptual Questions
246
CHAPTER
9
Genomics
&
Systems
_________
Biology
________________248
1.
Large-Scale Mapping with Sequence Tags
249
2.
Assembling Small Genomes by Shotgun
Sequencing
250
3.
Race for the Human Genome
253
4.
Survey of the Human Genome
255
5.
Pharmacogenomics—Genetically-
Individualized Drug Treatment
263
6.
Personal Genomics and Comparative
Genomics
264
7.
Bioinformatics and Computer Analysis
265
8.
Systems Biology
266
9.
Metagenomics and Community Sampling
268
10.
Epigenetics and Epigenomics
268
Key Concepts
269
Review Questions
271
Conceptual Questions
271
UNIT
3
The Central Dogma
of Molecular Biology
273
CHAPTER
10
Cell Division and
__________DNA
Replication
274
1.
Cell Division and Reproduction Are Not
Always Identical
275
2. DNA
Replication Occurs at the Replication Fork
275
3.
Properties of
DNA
Polymerase
279
4.
Nucleotides Are the Precursors for
DNA
Synthesis
280
5. DNA
Polymerase Elongates
DNA
Strands
282
6.
The Complete Replication Fork Is Complex
285
7.
Discontinuous Synthesis of the Lagging Strand
286
8.
Chromosome Replication Initiates at oriC
289
9.
Chromosome Replication Terminates at terC
292
10.
Cell Division in Bacteria Occurs after
Replication of Chromosomes
293
11.
The Concept of the
Replicón
297
12.
Replicating Linear
DNA
in Eukaryotes
298
13.
Cell Division in Higher Organisms
304
Key Concepts
305
Review Questions
307
Conceptual Questions
308
Contents xiii
CHAPTER
11
Transcription of
Genes
309
1.
Genes Are Expressed by Making
RNA
310
2.
How Is the Beginning of a Gene Recognized?
312
3.
Manufacturing the Message
314
4.
RNA Polymerase
Knows Where to Stop
316
5.
How Does the Cell Know Which Genes to
Turn On?
318
6.
Transcription in Eukaryotes Is More
Complex
324
Key Concepts
333
Review Questions
334
Conceptual Questions
335
CHAPTER
12
Processing of
RNA
336
1.
RNA
Is Processed in Several Ways
336
2.
Coding and Non-Coding
RNA
338
3.
Processing of Ribosomal and Transfer
RNA
338
4.
Eukaryotic Messenger
RNA
Contains a
Cap and a Tail
340
5.
Introns
Are Removed from
RNA
by Splicing
344
6.
Alternative Splicing Produces Multiple
Forms of
RNA
349
7.
Inteins
and Protein Splicing
352
8.
Base Modification of rRNA Requires Guide
RNA
355
9.
RNA
Editing Alters the Base Sequence
358
10.
Transport of
RNA
out of the Nucleus
360
11.
Degradation of mRNA
361
Key Concepts
366
Review Questions
367
Conceptual Questions
367
CHAPTER
13
Protein Synthesis
369
1.
Overview of Protein Synthesis
2.
Proteins Are Chains of
Amino
Acids
3.
Decoding the Genetic Information
4.
The RibosomeiThe Cell s Decoding Machine
5.
Three Possible Reading Frames Exist
6.
The tRNA Occupies Three Sites During
Elongation of the Polypeptide
7.
Bacterial mRNA Can Code for Several
Proteins
8.
Some Ribosomes Become Stalled and Are
Rescued
9.
Differences between Eukaryotic and
Prokaryotic Protein Synthesis
10.
Protein Synthesis Is Halted When Resources
Are Scarce
11.
A Signal Sequence Marks a Protein for
Export from the Cell
12.
Protein Synthesis Occurs
in Mitochondria and Chloroplasts
405
13.
Mistranslation Usually Results in Mistakes in
Protein Synthesis
407
14.
Many Antibiotics Work by Inhibiting Protein
Synthesis
408
15.
Post-Translational Modifications of Proteins
408
16.
Selenocysteine and Pyrrolysine: Rare
Amino
Acids
410
17.
Degradation of Proteins
412
Key Concepts
415
Review Questions
415
Conceptual Questions
416
CHAPTER
14
Protein Structure and
Function
ĄYI
1.
The Structure of Proteins Reflects Four Levels
of Organization
417
2.
Determining Protein Structures
428
3.
Nucleoproteins, Lipoproteins, and
Glycoproteins Are Conjugated Proteins
430
4.
Proteins Serve Numerous Cellular Functions
433
5.
Protein
(Nano)-Machines
436
6.
Enzymes Catalyze Metabolic Reactions
437
7.
Binding of Proteins to
DNA
Occurs in Several
Different Ways
450
8.
Denaturation of Proteins
454
Key Concepts
455
Review Questions
456
Conceptual Questions
457
CHAPTER
15
Proteomics: The Global
Analysis of Proteins
459
370
1.
The Proteome
460
371
2.
Antibodies Are Essential Proteomics Tools
464
376
3.
Western Blotting of Proteins
465
381
4.
Isolating Proteins with Chromatography
466
386
5.
Mass Spectrometry for Protein
Identification
468
389
6.
Protein-Tagging Systems
470
7.
Selection by Phage Display
474
394
8.
Protein Interactions: The Yeast Two-Hybrid
System
478
395
9.
Protein Interaction by
Co-Immunoprecipitation
483
397
10.
Protein Arrays
484
11.
Metabolomics
487
401
Key Concepts
489
Review Questions
490
403
Conceptual Questions
491
xiv Contents
Regulating
Gene
Expression
492
5
Regulation of
Transcription in
Prokaryotes
493
1.
tiene
Regulation Ensures a Physiological
Response
493
2.
Regulation at the Level of Transcription
Involves Several Steps
496
3.
Alternative Sigma Factors in Prokaryotes
Recognize Different Sets of Genes
497
4.
Activators and
Repressore
Participate in
Positive and Negative Regulation
502
5.
Two-Component Regulatory Systems
511
6.
Specific versus Global Control
515
7.
Accessory Factors and Nucleoid-Binding
Proteins
517
8.
Anti-Termination as a Control Mechanism
520
Key Concepts
523
Review Questions
524
Conceptual Questions
524
CHAPTER
17
Regulation of
Transcription in
Eukaryotes
526
1.
Transcriptional Regulation in Eukaryotes Is
More Complex Than in Prokaryotes
526
2.
Specific Transcription Factors Regulate
Protein-Encoding Genes
527
3.
Negative Regulation of Transcription Occurs in
Eukaryotes
533
4.
Heterochromatin Blocks Access to
DNA in
Eukaryotes
537
5.
Methylation of Eukaryotic
DNA
Controls
Gene Expression
543
6.
X-Chromosome Inactivation Occurs in
Female XX Animals
547
Key Concepts
550
Review Questions
550
Conceptual Questions
551
CHAPTER
18
Regulation at the
__________
RNA
Level
____________553
1.
Regulation at the Level of mRNA
553
2.
Basic Principles of
RNA
Interference (RNAi)
564
3.
Long Non-coding Regulatory
RNA
572
4.
CRISPR: Anti-Viral Defense in Bacteria
573
Premature Termination Causes Attenuation
of
RNA
Transcription
574
6.
Riboswitches
—
RNA
Acting Directly as a
Control Mechanism
576
Key Concepts
579
Review Questions
579
Conceptual Questions
580
CHAPTER
19
Analysis of Gene
Expression
581
1.
Monitoring Gene Expression
581
2.
Reporter Genes for Monitoring Gene
Expression
582
3.
Deletion Analysis of the Upstream Region
590
4.
DNA-Protein Complexes Can Be Isolated by
Chromatin Immunoprecipitation
594
5.
Location of the Start of Transcription by Primer
Extension
596
6.
Transcriptome
Analysis
600
7. DNA Microarrays
for Gene Expression
601
8.
TaqMan Quantitative PCR to Assay Gene
Expression
608
9.
Serial Analysis of Gene Expression (SAGE)
610
Key Concepts
613
Review Questions
613
Conceptual Questions
614
UNIT
5
Subcellular Life Forms
615
CHAPTER
20
Plasmids
616
1.
Plasmids as Replicons
616
2.
General Properties of Plasmids
619
3.
Plasmid
DNA
Replicates by Two Alternative
Methods
622
4.
Many Plasmids Help their Host Cells
628
5.
Plasmids may Provide Aggressive Characters
635
6.
Ή
Plasmids are Transferred from
Bacteria to Plants
640
7.
The
2μ
Plasmid of Yeast
644
8.
Certain
DNA
Molecules May Behave as
Viruses or Plasmids
645
Key Concepts
646
Review Questions
647
Conceptual Questions
648
CHAPTER
21
Viruses
__________________649
1.
Viruses Are Infectious Packages of Genetic
Information
650
2.
The Great Diversity of Viruses
658
Contents xv
3.
Viruses with
RNA
Genomes Have
Very Few Genes
4.
Retroviruses Use both
RNA
and
DNA
5.
Subviral
Infectious Agents
Key Concepts
Review Questions
Conceptual Questions
CHAPTER
22
Mobile
DNA
666
672
678
684
684
685
686
1.
Subcellular Genetic Elements as Gene
Creatures
686
2.
Most Mobile
DNA
Consists of Transposable
Elements
687
3.
Retroelements Make an
RNA
Copy
703
4.
The Multitude of Transposable Elements
708
5.
Junk
DNA
and Selfish
DNA 716
Key Concepts
717
Review Questions
718
Conceptual Questions
719
UNIT
6
Changing the
DNA
Blueprint
720
CHAPTER
23
Mutations and Repair
721
1.
Mutations Alter the
DNA
Sequence
721
2.
The Major Types of Mutation
722
3.
Chemical
Mutagens
Damage
DNA 733
4.
Overview of
DNA
Repair
742
5.
Mutations Occur More Frequently at Hotspots
758
6.
Reversions Are Genetic Alterations That
Change the Phenotype Back to Wild-Type
759
7.
Site-Directed Mutagenesis
763
Key Concepts
764
Review Questions
765
Conceptual Questions
766
CHAPTER
24
Recombination
_________767
1.
Overview of Recombination
767
2.
Molecular Basis of Homologous Recombination
769
3.
Site-Specific Recombination
773
4.
Recombination in Higher Organisms
776
5.
Gene Conversion
778
Key Concepts
781
Review Questions
781
Conceptual Questions
782
CHAPTER
25
Bacterial Genetics
783
1.
Reproduction versus Gene Transfer
783
2.
Fate of the Incoming
DNA
after Uptake
784
3.
Transformation Is Gene Transfer by Naked
DNA 785
4.
Gene Transfer by Virus—Transduction
792
5.
Transfer of Plasmids between Bacteria
795
6.
Gene Transfer among Gram-Positive Bacteria
801
7.
Archaeal Genetics
804
8.
Whole-Genome Sequencing
805
Key Concepts
808
Review Questions
809
Conceptual Questions
810
CHAPTER
26
Molecular Evolution
812
1.
Getting Started—Formation of the Earth
812
2.
Oparin s Theory of the Origin of Life
814
3.
Origin of Informational Macromolecules
818
4.
The Autotrophic Theory of the Origin of
Metabolism
823
5.
Evolution of
DNA, RNA,
and Protein Sequences
824
6.
Different Proteins Evolve at Very Different Rates
830
7.
Symbiotic Origin of Eukaryotic Cells
835
8. DNA
Sequencing and Biological Classification
841
9.
Evolving Sideways: Horizontal Gene Transfer
847
Key Concepts
850
Review Questions
851
Conceptual Questions
852
Glossary
855
Index
883
|
| any_adam_object | 1 |
| author | Clark, David P. 1952- Pazdernik, Nanette Jean |
| author_GND | (DE-588)131505998 (DE-588)136402100 |
| author_facet | Clark, David P. 1952- Pazdernik, Nanette Jean |
| author_role | aut aut |
| author_sort | Clark, David P. 1952- |
| author_variant | d p c dp dpc n j p nj njp |
| building | Verbundindex |
| bvnumber | BV040106470 |
| classification_rvk | WD 4150 WG 1700 |
| ctrlnum | (OCoLC)796206252 (DE-599)OBVAC08926147 |
| dewey-full | 572.8 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8 |
| dewey-search | 572.8 |
| dewey-sort | 3572.8 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| genre | (DE-588)4123623-3 Lehrbuch gnd-content |
| genre_facet | Lehrbuch |
| id | DE-604.BV040106470 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:17:00Z |
| institution | BVB |
| isbn | 9780123785947 0123785944 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024962900 |
| oclc_num | 796206252 |
| open_access_boolean | |
| owner | DE-20 DE-1028 DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-188 |
| owner_facet | DE-20 DE-1028 DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-188 |
| physical | XV, 907 S. Ill., graph. Darst. 28 cm |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| spelling | Clark, David P. 1952- Verfasser (DE-588)131505998 aut Molecular biology David P. Clark ; Nanette J. Pazdernik 2. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2013 XV, 907 S. Ill., graph. Darst. 28 cm txt rdacontent n rdamedia nc rdacarrier Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Molecular biology. Molecular genetics. (DE-588)4123623-3 Lehrbuch gnd-content Molekularbiologie (DE-588)4039983-7 s DE-604 Molekulargenetik (DE-588)4039987-4 s Pazdernik, Nanette Jean Verfasser (DE-588)136402100 aut Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024962900&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Clark, David P. 1952- Pazdernik, Nanette Jean Molecular biology Molekulargenetik (DE-588)4039987-4 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4039987-4 (DE-588)4039983-7 (DE-588)4123623-3 |
| title | Molecular biology |
| title_auth | Molecular biology |
| title_exact_search | Molecular biology |
| title_full | Molecular biology David P. Clark ; Nanette J. Pazdernik |
| title_fullStr | Molecular biology David P. Clark ; Nanette J. Pazdernik |
| title_full_unstemmed | Molecular biology David P. Clark ; Nanette J. Pazdernik |
| title_short | Molecular biology |
| title_sort | molecular biology |
| topic | Molekulargenetik (DE-588)4039987-4 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | Molekulargenetik Molekularbiologie Lehrbuch |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024962900&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT clarkdavidp molecularbiology AT pazderniknanettejean molecularbiology |