Atomic force microscopy in liquid: biological applications
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Weinheim
Wiley-VCH
2012
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| Physical Description: | XIX, 362 S. Ill., graph. Darst. |
| ISBN: | 9783527327584 9783527649808 |
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| 245 | 1 | 0 | |a Atomic force microscopy in liquid |b biological applications |c ed. by Arturo M. Baró ... |
| 264 | 1 | |a Weinheim |b Wiley-VCH |c 2012 | |
| 300 | |a XIX, 362 S. |b Ill., graph. Darst. | ||
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IMAGE 1
CONTENTS
PREFACE X I I I
LIST O F CONTRIBUTORS XV
PART I CENERAL ATOMIC FORCE MICROSCOPY 1
1 AFM: BASIC CONCEPTS 3
FERNANDO MORENO-HERRERO A N D JULIO GOMEZ-HERRERO
1.1 ATOMIC FORCE MICROSCOPE: PRINCIPLES 3
1.2 PIEZOELECTRIC S C A N N E R S 5
1.2.1 PIEZOELECTRIC S C A N N E R S FOR I M A G I N G IN LIQUIDS 8
1.3 T I P S A N D CANTILEVERS 8
1.3.1 CANTILEVER CALIBRATION 10
1.3.2 TIPS A N D CANTILEVERS FOR I M A G I N G I N LIQUIDS 11
1.3.3 CANTILEVER DYNAMICS I N LIQUIDS 13
1.4 FORCE DETECTION M E T H O D S FOR I M A G I N G I N LIQUIDS 15
1.4.1 PIEZOELECTRIC CANTILEVERS A N D T U N I N G FORKS 15
1.4.2 LASER B E A M DEFLECTION M E T H O D 17
1.4.2.1 LIQUID CELLS A N D B E A M DEFLECTION 18
1.5 AFM OPERATION MODES'. CONTACT, J U M P I N G / P U L S E D , D Y N A
M I C 19
1.5.1 CONTACT M O D E 19
1.5.2 J U M P I N G A N D P U L S E D FORCE M O D E 2 0
1.5.3 DYNAMIC MODES 2 2
1.5.3.1 LIQUID CELLS A N D DYNAMIC MODES 2 3
1.6 T H E FEEDBACK LOOP 2 4
1.7 I M A G E REPRESENTATION 2 5
1.8 ARTIFACTS A N D RESOLUTION LIMITS 28
1.8.1 ARTIFACTS RELATED TO T H E G E O M E T R Y O F T H E T I P 2 8
1.8.2 ARTIFACTS RELATED TO T H E FEEDBACK LOOP 3 0
1.8.3 RESOLUTION LIMITS 31
ACKNOWLEDGMENTS 3 2
REFERENCES 3 2
HTTP://D-NB.INFO/1016558414
IMAGE 2
V I CONTENTS
2 CARBON N A N O T U B E TIPS IN ATOMIC FORCE MICROSCOPY WITH
APPLICATIONS
T O IMAGING IN LIQUID 3 5
EDWARD D. DE ASIS, JR., JOSEPH LEUNG, A N D CATTIEN V. NGUYEN 2.1
INTRODUCTION 3 5
2.2 FABRICATION O F C N T A F M PROBES 3 7
2.2.1 MECHANICAL A T T A C H M E N T 3 8
2.2.2 C N T A T T A C H M E N T T E C H N I Q U E S EMPLOYING MAGNETIC A
N D ELECTRIC FIELDS 3 9
2.2.3 DIRECT G R O W T H O F C N T TIPS 41
2.2.4 E M E R G I N G C N T A T T A C H M E N T T E C H N I Q U E S 4 3
2.2.5 POSTFABRICATION MODIFICATION O F T H E C N T T I P 43
2.2.5.1 S H O R T E N I N G 43
2.2.5.2 COATING W I T H METAL 4 4
2.3 CHEMICAL FUNCTIONALIZATION 4 4
2.3.1 FUNCTIONALIZATION O F T H E C N T FREE E N D 4 5
2.3.2 COATING T H E C N T SIDEWALL 4 5
2.4 MECHANICAL PROPERTIES O F CNTS I N RELATION TO A F M APPLICATIONS 4
6
2.4.1 C N T ATOMIC STRUCTURE 4 7
2.4.2 MECHANICAL PROPERTIES O F C N T AFM TIPS 4 9
2.5 DYNAMICS O F C N T TIPS I N LIQUID 5 0
2.5.1 INTERACTION O F MICROFABRICATED A F M TIPS A N D CANTILEVERS I N
LIQUID 5 0
2.5.2 C N T A F M TIPS I N LIQUID 5 2
2.5.3 INTERACTION O F C N T W I T H LIQUIDS 5 2
2.5.3.1 C N T TIPS A T T H E A I R - L I Q U I D INTERFACE D U R I N G A
P P R O A C H 5 4
2.5.3.2 C N T TIPS A T T H E L I Q U I D - S O L I D INTERFACE 5 6
2.5.3.3 C N T TIPS A T T H E A I R - L I Q U I D INTERFACE D U R I N G
WITHDRAWAL 58
2.6 P E R F O R M A N C E A N D RESOLUTION O F C N T TIPS I N LIQUID 58
2.6.1 P E R F O R M A N C E O F C N T A F M TIPS W H E N I M A G I N G I
N LIQUID 5 8
2.6.2 BIOLOGICAL I M A G I N G I N LIQUID M E D I U M WITH C N T AFM
TIPS 5 9
2.6.3 CELL M E M B R A N E PENETRATION A N D APPLICATIONS O F
INTRACELLULAR C N T
AFM PROBES 6 0
REFERENCES 61
3 FORCE SPECTROSCOPY 6 5
ARTURO M. BARE5
3.1 INTRODUCTION 6 5
3.2 M E A S U R E M E N T O F FORCE CURVES 6 7
3.2.1 ANALYSIS O F FORCE CURVES T A K E N I N AIR 6 8
3.2.2 ANALYSIS O F FORCE CURVES I N A LIQUID 70
3.3 M E A S U R I N G SURFACE FORCES BY T H E SURFACE FORCE A P P A R A
T U S 70
3.4 FORCES B E T W E E N MACROSCOPIC BODIES 71
3.5 THEORY O F DLVO FORCES B E T W E E N T W O SURFACES 71
3.6 V A N D E R WAALS FORCES - T H E H A M A K E R C O N S T A N T 72
3.7 ELECTROSTATIC FORCE B E T W E E N SURFACES I N A LIQUID 72
IMAGE 3
CONTENTS I V I I
3.8 SPATIALLY RESOLVED FORCE SPECTROSCOPY 76
3.9 FORCE SPECTROSCOPY I M A G I N G O F SINGLE D N A MOLECULES 7 8
3.10 SOLVATION FORCES 7 9
3.11 HYDROPHOBIC FORCES 81
3.12 STERIC FORCES 81
3.13 CONCLUSIVE R E M A R K S 83
A C K N O W L E D G M E N T S 83 REFERENCES 8 3
4 DYNAMIC-MODE AFM IN LIQUID 8 7
TAKESHI F U K U M A A N D MICHAEL J. HIGGINS 4.1 INTRODUCTION 8 7
4.2 OPERATION PRINCIPLES 8 8
4.2.1 A M P L I T U D E A N D P H A S E MODULATION A F M (AM- A N D
PM-AFM) 88
4.2.2 FREQUENCY-MODULATION A F M (FM-AFM) 8 9
4.3 I N S T R U M E N T A T I O N 90
4.3.1 CANTILEVER EXCITATION 9 0
4.3.2 CANTILEVER DEFLECTION M E A S U R E M E N T 91
4.3.3 O P E R A T I N G CONDITIONS 93
4.3.4 AM-AFM 93
4.3.4.1 FM-AFM 95
4.3.4.2 PM-AFM 96
4.4 QUANTITATIVE FORCE M E A S U R E M E N T S 9 7
4.4.1 CALIBRATION O F S P R I N G C O N S T A N T 9 8
4.4.2 CONSERVATIVE A N D DISSIPATIVE FORCES 101
4.4.3 SOLVATION FORCE M E A S U R E M E N T S 103
4.4.3.1 INORGANIC SOLIDS I N NONPOLAR LIQUIDS 104
4.4.3.2 M E A S U R E M E N T S I N P U R E W A T E R 106
4.4.3.3 SOLVATION FORCES I N BIOLOGICAL SYSTEMS 106
4.4.4 SINGLE-MOLECULE FORCE SPECTROSCOPY 108
4.4.4.1 U N F O L D I N G A N D "STRETCHING" O F BIOMOLECULES 108
4.4.4.2 L I G A N D - R E C E P T O R INTERACTIONS 110
4.5 HIGH-RESOLUTION I M A G I N G 110
4.5.1 SOLID CRYSTALS 112
4.5.2 BIOMOLECULAR ASSEMBLIES 113
4.5.3 W A T E R DISTRIBUTION 114
4.6 S U M M A R Y A N D F U T U R E PROSPECTS 116
REFERENCES 117
5 FUNDAMENTALS O F AFM CANTILEVER DYNAMICS IN LIQUID
ENVIRONMENTS 121 DANIEL KIRACOFE, J O H N MELCHER, A N D ARVIND R A M A
N 5.1 INTRODUCTION 121
5.2 REVIEW O F F U N D A M E N T A L S O F CANTILEVER OSCILLATION 122
5.3 H Y D R O D Y N A M I C S O F CANTILEVERS I N LIQUIDS 123
IMAGE 4
V I I I I CONTENTS
5.4 METHODS O F DYNAMIC EXCITATION 126
5.4.1 REVIEW O F CANTILEVER EXCITATION M E T H O D S 128
5.4.2 THEORY 130
5.4.2.1 DIRECT FORCING 130
5.4.2.2 IDEAL PIEZO/ACOUSTIC 132
5.4.2.3 T H E R M A L 132
5.4.2.4 COMPARISON O F EXCITATION M E T H O D S 133
5.4.3 PRACTICAL CONSIDERATIONS FOR ACOUSTIC M E T H O D 135
5.4.4 PHOTOTHERMAL M E T H O D 137
5.4.5 FREQUENCY MODULATION CONSIDERATIONS I N LIQUIDS 140
5.5 DYNAMICS O F CANTILEVERS INTERACTING WITH S A M P L E S I N LIQUIDS
140
5.5.1 EXPERIMENTAL OBSERVATIONS O F OSCILLATING PROBES INTERACTING WITH
SAMPLES I N LIQUIDS 141 5.5.2 MODELING A N D N U M E R I C A L
SIMULATIONS O F OSCILLATING P R O B E S INTERACTING W I T H S A M P L E
S I N LIQUIDS 142 5.5.3 COMPOSITIONAL M A P P I N G I N LIQUIDS 145
5.5.4 IMPLICATIONS FOR FORCE SPECTROSCOPY I N LIQUIDS 148
5.6 OUTLOOK 150
REFERENCES 150
6 SINGLE-MOLECULE FORCE SPECTROSCOPY 157
ALBERT GALERA-PRAT, RODOLFO HERMANS, RUBEN HERVAS, ANGEL GDMEZ-SICILIA,
AND MARIANO CARRION-VDZQUEZ 6.1 I N T R O D U C T I O N 157
6.1.1 W H Y SINGLE-MOLECULE FORCE SPECTROSCOPY? 157
6.1.2 SMFS I N BIOLOGY 158
6.1.3 SMFS T E C H N I Q U E S A N D RANGES 158
6.2 AFM-SMFS PRINCIPLES 159 ,
6.2.1 LENGTH-CLAMP M O D E 160
6.2.2 FORCE-CLAMP M O D E 163
6.3 D Y N A M I C S O F ADHESION BONDS 165
6.3.1 B O N D DISSOCIATION DYNAMICS I N LENGTH C L A M P 165
6.3.2 G E N E R A L CONSIDERATIONS 167
6.3.3 B O N D DISSOCIATION DYNAMICS I N FORCE C L A M P 168
6.3.3.1 T H E NEED FOR ROBUST STATISTICS 169
6.4 SPECIFIC VERSUS O T H E R INTERACTIONS 169
6.4.1 INTRAMOLECULAR SINGLE-MOLECULE MARKERS 170
6.4.1.1 T H E W O R M L I K E CHAIN: A N ELASTICITY MODEL 170
6.4.1.2 PROTEINS 171
6.4.1.3 DNA A N D POLYSACCHARIDES 174
6.4.2 INTERMOLECULAR SINGLE-MOLECULE MARKERS 174
6.5 STEERED MOLECULAR DYNAMICS SIMULATIONS 176
6.6 BIOLOGICAL FINDINGS U S I N G A F M - S M F S 177
6.6.1 TITIN AS A N ADJUSTABLE MOLECULAR S P R I N G I N T H E MUSCLE
SARCOMERE 177
IMAGE 5
CONTENTS I X
6.6.2 MONITORING T H E FOLDING PROCESS BY FORCE-CLAMP SPECTROSCOPY 180
6.6.3 INTERMOLECULAR BINDING FORCES A N D ENERGIES I N PAIRS O F
BIOMOLECULES 180
6.6.4 N E W INSIGHTS I N CATALYSIS REVEALED AT T H E SINGLE-MOLECULE
LEVEL 181 6.7 C O N C L U D I N G R E M A R K S 182
A C K N O W L E D G M E N T S 182 DISCLAIMER 182
REFERENCES 182
7 HIGH-SPEED AFM FOR OBSERVING DYNAMIC PROCESSES IN LIQUID 189
TOSHIO ANDO, TAKAYUKI UCHIHASHI, NORIYUKI KODERA, MIKIHIRO SHIBATA,
DAISUKE YAMAMOTO, A N D HAYATO YAMASHITA 7.1 INTRODUCTION 189
7.2 THEORETICAL DERIVATION O F I M A G I N G RATE A N D FEEDBACK
BANDWIDTH 190
7.2.1 I M A G I N G T I M E A N D FEEDBACK BANDWIDTH 190
7.2.2 T I M E DELAYS 191
7.3 T E C H N I Q U E S REALIZING HIGH-SPEED BIO-AFM 192
7.3.1 SMALL CANTILEVERS 192
7.3.2 FAST A M P L I T U D E DETECTOR 194
7.3.3 HIGH-SPEED S C A N N E R 194
7.3.4 ACTIVE D A M P I N G T E C H N I Q U E S 196
7.3.5 S U P P R E S S I O N O F P A R A C H U T I N G 198
7.3.6 FAST P H A S E DETECTOR 199
7.4 SUBSTRATE SURFACES 200
7.4.1 S U P P O R T E D PLANAR LIPID BILAYERS 200
7.4.1.1 CHOICE O F ALKYL C H A I N S 201
7.4.1.2 CHOICE O F H E A D G R O U P S 201
7.4.2 STREPTAVIDIN 2D CRYSTAL SURFACE 201
7.5 I M A G I N G O F D Y N A M I C MOLECULAR PROCESSES 203
7.5.1 BACTERIORHODOPSIN CRYSTAL EDGE 203
7.5.2 PHOTOACTIVATION O F BACTERIORHODOPSIN 204
7.6 F U T U R E PROSPECTS O F HIGH-SPEED AFM 2 0 6
7.6.1 I M A G I N G RATE A N D LOW INVASIVENESS 206
7.6.2 HIGH-SPEED AFM C O M B I N E D WITH FLUORESCENCE MICROSCOPE 206
7.7 CONCLUSION 207
REFERENCES 207
8 INTEGRATION O F AFM WITH OPTICAL MICROSCOPY TECHNIQUES 211
Z H C SUN, ANDREEA TRACHE, KENITH MEISSNER, A N D GERALD A. MEININGER
8.1 INTRODUCTION 211
8.1.1 C O M B I N I N G AFM W I T H FLUORESCENCE MICROSCOPY 214
8.1.1.1 EPIFLUORESCENCE MICROSCOPY 214
8.1.2 EXAMPLES O F APPLICATIONS 215
8.1.2.1 C A 2 + F L U O R E S C E N C E MICROSCOPY 215
8.1.2.2 AFM - EPIFLUORESCENCE MICROSCOPY 217
IMAGE 6
X CONTENTS
8.2 C O M B I N I N G A F M W I T H IRM A N D TIRF MICROSCOPY 217
8.2.1 INTERFERENCE REFLECTION MICROSCOPY 217
8.2.1.1 OPTICAL S E T U P 218
8.2.2 TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY 218
8.2.2.1 OPTICAL S E T U P 218
8.2.2.2 APPLICATIONS O F C O M B I N E D A F M - T I R F A N D A F M - I
R M MICROSCOPY 2 2 0 8.3 C O M B I N I N G A F M A N D FRET 221
8.3.1 FRET 221
8.3.2 FRET A N D NEAR-FIELD S C A N N I N G OPTICAL MICROSCOPY (NSOM) 2
2 2
8.4 FRET-AFM 2 2 2
8.5 S A M P L E PREPARATION A N D EXPERIMENT SETUP 223
8.5.1 CELL CULTURE, TRANSFECTION, A N D FURA-LOADING 223
8.5.2 CANTILEVER PREPARATION 224
8.5.3 TYPICAL EXPERIMENTAL PROCEDURE 225
REFERENCES 2 2 5
PART II BIOLOGICAL APPLICATIONS 231
9 AFM IMAGING IN LIQUID O F DNA A N D P R O T E I N - D N A COMPLEXES
233
YURI L. LYUBCHENKO
9.1 OVERVIEW: T H E STUDY O F DNA AT NANOSCALE RESOLUTION 233
9.2 S A M P L E PREPARATION FOR AFM I M A G I N G O F D N A A N D P R O
T E I N - D N A
COMPLEXES 234
9.3 A F M O F D N A I N A Q U E O U S SOLUTIONS 236
9.3.1 ELEVATED RESOLUTION I N A Q U E O U S SOLUTIONS 236
9.3.2 S E G M E N T A L MOBILITY O F DNA 237
9.4 A F M I M A G I N G O F ALTERNATIVE DNA C O N F O R M A T I O N S
239
9.4.1 C R U C I F O R M S I N DNA 239
9.4.2 INTRAMOLECULAR TRIPLE HELICES 244
9.4.3 FOUR-WAY D N A JUNCTIONS A N D DNA RECOMBINATION 245
9.5 D Y N A M I C S O F P R O T E I N - D N A INTERACTIONS 247
9.5.1 SITE-SPECIFIC P R O T E I N - D N A COMPLEXES 247
9.5.2 C H R O M A T I N DYNAMICS TIME-LAPSE AFM 251
9.6 D N A C O N D E N S A T I O N 253
9.7 CONCLUSIONS 254
A C K N O W L E D G M E N T S 254 REFERENCES 2 5 5
10 STABILITY O F LIPID BILAYERS A S MODEL MEMBRANES: ATOMIC FORCE
MICROSCOPY A N D SPECTROSCOPY APPROACH 259 LORENA REDONDO-MORATA, MARINA
IN6S GIANNOTTI, A N D FAUSTO S A N Z 10.1 BIOLOGICAL M E M B R A N E S 2
5 9
10.1.1 CELL M E M B R A N E 259
10.1.2 SUPPORTED LIPID BILAYERS 2 5 9
IMAGE 7
CONTENTS | X I
10.2 MECHANICAL CHARACTERIZATION O F LIPID M E M B R A N E S 263
10.2.1 BREAKTHROUGH FORCE AS A MOLECULAR FINGERPRINT 263
10.2.2 A F M TIP-LIPID BILAYER INTERACTION 2 6 5
10.2.3 EFFECT O F CHEMICAL C O M P O S I T I O N O N T H E MECHANICAL
STABILITY O F LIPID BILAYERS 2 6 7
10.2.4 EFFECT O F IONIC STRENGTH O N T H E MECHANICAL STABILITY O F
LIPID BILAYERS 268
10.2.5 EFFECT O F DIFFERENT CATIONS O N T H E MECHANICAL STABILITY O F
LIPID BILAYERS 271
10.2.6 EFFECT O F T E M P E R A T U R E O N T H E MECHANICAL STABILITY O
F LIPID BILAYERS 273
10.2.7 T H E C A S E O F PHASE-SEGREGATED LIPID BILAYERS 2 7 4
10.3 F U T U R E PERSPECTIVES 2 7 9
REFERENCES 279
H SINGLE-MOLECULE ATOMIC FORCE MICROSCOPY O F CELLULAR S E N S O R S 2 8
5
JTIRGEN J. HEINISCH A N D YVES F. DUFRENE 11.1 INTRODUCTION 285
11.1.1 M E C H A N O S E N S O R S I N LIVING CELLS 285
11.1.2 YEAST CELL WALL INTEGRITY SENSORS: A VALUABLE MODEL FOR M E C H A
N O S E N S I N G 286 11.2 METHODS 288
11.2.1 ATOMIC FORCE MICROSCOPY O F LIVE CELLS 288
11.2.2 AFM DETECTION O F SINGLE S E N S O R S 2 9 0
11.2.3 BRINGING YEAST S E N S O R S T O T H E SURFACE 291
11.3 P R O B I N G SINGLE YEAST SENSORS I N LIVE CELLS 2 9 2
11.3.1 M E A S U R I N G S E N S O R S P R I N G PROPERTIES 292
11.3.2 I M A G I N G S E N S O R CLUSTERING 2 9 5
11.3.3 U S I N G S E N S O R S AS MOLECULAR RULERS 298
11.4 CONCLUSIONS 3 0 2
A C K N O W L E D G M E N T S 303 REFERENCES 303
12 AFM-BASED SINGLE-CELL FORCE SPECTROSCOPY 3 0 7
CLEMENS M. FRANZ A N D A N N A TAUBENBERGER 12.1 INTRODUCTION 307
12.2 CANTILEVER CHOICE 3 1 0
12.3 CANTILEVER FUNCTIONALIZATION 3 1 0
12.4 CANTILEVER CALIBRATION 311
12.5 CELL A T T A C H M E N T TO T H E AFM CANTILEVER 311
12.6 RECORDING A F O R C E - D I S T A N C E CURVE 313
12.7 PROCESSING F - D CURVES 3 1 5
12.8 QUANTIFYING OVERALL CELL A D H E S I O N BY SCFS 317
12.9 SFCS WITH SINGLE-MOLECULE RESOLUTION 3 2 0
12.10 DYNAMIC FORCE SPECTROSCOPY 321
IMAGE 8
X I I I CONTENTS
12.11 MEASURING CELL-CELL ADHESION 325
12.12 CONCLUSIONS A N D OUTLOOK 326
REFERENCES 327
13 NANOSURGICAL MANIPULATION O F LIVING CELLS WITH T H E AFM 331
ATSUSHI IKAI, REHANA AFRIN, TAKAHIRO WATANABE-NAKAYAMA, AND SHIN-ICHI
MACHIDA 13.1 INTRODUCTION: MECHANICAL MANIPULATION O F LIVING CELLS 331
13.2 BASIC MECHANICAL PROPERTIES O F PROTEINS A N D CELLS 331
13.3 HOLE FORMATION O N T H E CELL M E M B R A N E 3 3 2
13.4 EXTRACTION O F M R N A F R O M LIVING CELLS 334
13.5 DNA DELIVERY A N D G E N E EXPRESSION 3 3 5
13.6 MECHANICAL MANIPULATION O F INTRACELLULAR STRESS FIBERS 338
13.6.1 AFM U S E D AS A LATERAL FORCE MICROSCOPE 338
13.6.2 FORCE CURVES A N D FLUORESCENCE I M A G E S U N D E R LATERAL
FORCE APPLICATION 3 4 0
13.6.2.1 CASE 1 3 4 0
13.6.2.2 CASE 2 3 4 0
13.7 CELLULAR ADAPTATION TO LOCAL STRESSES 343
13.8 APPLICATION O F C A R B O N N A N O T U B E NEEDLES 344
13.9 U S E O F FABRICATED AFM PROBES WITH A H O O K I N G FUNCTION 346
13.9.1 RESULT FOR A SEMI-INTACT CELL 348
13.9.2 RESULT FOR A LIVING CELL 348
13.10 M E M B R A N E PROTEIN EXTRACTION 348
13.11 F U T U R E PROSPECTS 3 5 0
A C K N O W L E D G M E N T S 3 5 0 REFERENCES 3 5 0
INDEX 3 5 5 |
| any_adam_object | 1 |
| author2 | Baró, Arturo M. |
| author2_role | edt |
| author2_variant | a m b am amb |
| author_facet | Baró, Arturo M. |
| building | Verbundindex |
| bvnumber | BV039958781 |
| classification_rvk | UH 6320 |
| ctrlnum | (OCoLC)794533416 (DE-599)DNB1016558414 |
| dewey-full | 502.82 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 502 - Miscellany |
| dewey-raw | 502.82 |
| dewey-search | 502.82 |
| dewey-sort | 3502.82 |
| dewey-tens | 500 - Natural sciences and mathematics |
| discipline | Allgemeine Naturwissenschaft Physik |
| format | Book |
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| id | DE-604.BV039958781 |
| illustrated | Illustrated |
| indexdate | 2024-07-21T00:27:35Z |
| institution | BVB |
| isbn | 9783527327584 9783527649808 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024816556 |
| oclc_num | 794533416 |
| open_access_boolean | |
| owner | DE-11 DE-703 DE-19 DE-BY-UBM |
| owner_facet | DE-11 DE-703 DE-19 DE-BY-UBM |
| physical | XIX, 362 S. Ill., graph. Darst. |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Wiley-VCH |
| record_format | marc |
| spelling | Atomic force microscopy in liquid biological applications ed. by Arturo M. Baró ... Weinheim Wiley-VCH 2012 XIX, 362 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Rasterkraftmikroskopie (DE-588)4274473-8 gnd rswk-swf Flüssigkeit (DE-588)4017621-6 gnd rswk-swf Rasterkraftmikroskopie (DE-588)4274473-8 s Flüssigkeit (DE-588)4017621-6 s DE-604 Baró, Arturo M. edt Erscheint auch als Online-Ausgabe, EPUB 978-3-527-64982-2 Erscheint auch als Online-Ausgabe, MOBI 978-3-527-64981-5 Erscheint auch als Online-Ausgabe, PDF 978-3-527-64983-9 X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3902513&prov=M&dok_var=1&dok_ext=htm Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024816556&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Atomic force microscopy in liquid biological applications Rasterkraftmikroskopie (DE-588)4274473-8 gnd Flüssigkeit (DE-588)4017621-6 gnd |
| subject_GND | (DE-588)4274473-8 (DE-588)4017621-6 |
| title | Atomic force microscopy in liquid biological applications |
| title_auth | Atomic force microscopy in liquid biological applications |
| title_exact_search | Atomic force microscopy in liquid biological applications |
| title_full | Atomic force microscopy in liquid biological applications ed. by Arturo M. Baró ... |
| title_fullStr | Atomic force microscopy in liquid biological applications ed. by Arturo M. Baró ... |
| title_full_unstemmed | Atomic force microscopy in liquid biological applications ed. by Arturo M. Baró ... |
| title_short | Atomic force microscopy in liquid |
| title_sort | atomic force microscopy in liquid biological applications |
| title_sub | biological applications |
| topic | Rasterkraftmikroskopie (DE-588)4274473-8 gnd Flüssigkeit (DE-588)4017621-6 gnd |
| topic_facet | Rasterkraftmikroskopie Flüssigkeit |
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