PCR technology: current innovations
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| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Boca Raton [u.a.]
CRC Press
2013
|
| Edition: | 3. ed. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Klappentext |
| Item Description: | 2. Aufl. u.d.T.: PCR technology/Weißensteiner, Thomas |
| Physical Description: | XXI, 435 S. Ill., graph. Darst. |
| ISBN: | 9781439848050 |
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| adam_text | LIFE SCIENCES
Third Edition
PCR Technology
Current Innovations
PCR s simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation
that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate
concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel
methods, insights, and points of view that provides a critical and timely reference point for anyone wishing
to use this technology.
Topics in this forward-thinking volume include
The purification and handling of PCR templates
The effect of the manufacture and purification of the oligonucleotide on PCR behavior
Optimum buffer composition
Probe options
The design and optimization of
q PCR
assays
Issues surrounding the development and refinement of instrumentation
•
Effective controls to protect against uncertainties due to reaction variability
Covering all aspects of PCR and real-time PCR, the book contains detailed protocols that make it
suitable as both a reference and an instruction manual. Each chapter presents detailed guidelines as
well as helpful hints and tips supplied by authors who are recognized experts in their fields. In addition
to descriptions of current technology and best practices, the book also provides information about new
developments in the PCR arena.
Contents
Prel
асе
...............................................................................................................................................ix
Editors..............................................................................................................................................xv
Contributors
...................................................................................................................................xvii
PART I Template Preparation
Chapter
1
Pre-PCR Processing Strategies
....................................................................................3
Johannes
Hedman,
Maria
Lövenklev,
Petra
Wolffs,
Charlotta Löfström,
Rickard
Knutsson,
and Peter
Rådström
Chapter
2
Use of a Liposomal Internal Control Vehicle for Whole-Process Quality
Assurance of Nucleic Acid Amplification
..................................................................19
Einar
S.
Berg and
Tania
Nolan
PART
Π
Reaction Components
Chapter
3
Oligonucleotide Synthesis and Purification
...............................................................39
Yannick
Fillon,
Theresa Creasey, Qin Xiang, and Carlos Martinez
Chapter
4
Augmenting PCR: Effects of Additives in the Polymerase Chain Reaction
..............57
Ernest J. Mueller
Chapter
5
Real-Time Fluorescent PCR by Labeled Primer with a Single Fluorescent
Molecule
.....................................................................................................................63
Hong Yan and Liu Xiao
Кип
Chapter
6
Using Scorpion Primers for Genotyping
....................................................................75
David Whitcombe
Chapter
7
LNA™:
Adding New Functionality to PCR
...............................................................87
Ѕфгеп
M.
Echwald,
Ditte Andreasen,
and Peter Mouritzen
Chapter
8
Modified dNTPs: A Toolbox for Use in PCR
..........................................................103
Natasha Paul,
Olga
Adelfinskaya, Elena Hidalgo Ashrafi, Tony
Le,
and
Sabrina
Shore
vi
Contents
PART III Instruments
Chapter
9
Thermocycler Calibration and Analytical Assay Validation
...................................125
Mary Span, Marc Verblakt, and Tom Hendrikx
Chapter
10 Ultra-High-Speed PCR
Instrument Development
....................................................143
Nick Burroughs and Emmanouil
Karteris
Chapter
11
Dropletization of Bioreactions: How Single-Cell Quantitative Analyses May
Improve Gene Measurement and Cancer Diagnosis
................................................159
Ehsan Karimiani, Amelia Markey, and Philip Day
PART IV Design, Optimization, QC, and Standardization
Chapter
12
Assay Design for Real-Time qPCR
..........................................................................177
Gregory L. Shipley
Chapter
13
Primer Design for Large-Scale Multiplex PCR and Arrayed Primer Extension
.....199
Muido
Remm, Kaarel
Krjutškov,
and Andres Metspalu
Chapter
14
Development and Use of qPCR Assays for Detection and Study of Neglected
Tropical and Emerging Infectious Diseases
.............................................................209
Ashley R. Heath, Norha Deluge, Maria
Galli de Amorini,
and Emmanuel Dias-Neto
Chapter
15
MIQE: Guidelines for Reliable Design and Transparent Reporting of
Real-Time PCR
Assays
............................................................................................221
Tania
Nolan and Stephen A. Bustin
PARTV Data Analysis
Chapter
16
Hypothesis-Driven Approaches to Multivariate Analysis of qPCR Data
................233
Anders
Bergkvist,
Azam Sheikh Muhammad, and Peter Damaschke
Chapter
17
Experiment Design, Data Management, and Univariate Statistical Analysis
of Gene-Expression Data Obtained by
Real-Time
Quantitative PCR
.....................245
Ales Tichopad. Tzachi Bar,
Malin Edling, Boel Svanberg,
Tania
Nolan,
and Anders
Bergkvist
Chapter
18
Biomarker
Discovery via RT-qPCR and Bioinformatical Validation
......................259
Christiane
Becker,
Irmgard
Riedmaier, and Michael W. Pfaffl
Contents
vii
Chapter
19
Optimization of Quantitative
Real-Time PCR
for Studies in Cartilage
Mechanobiology
.......................................................................................................271
Oto
Akanji, Donald
Salter,
and Tina Chowdhury
PART VI Applications
Chapter
20
Enriching
DNA
Sequences with Nucleotide Variation by Thymidine
Glycosylases Combined with Suppression PCR
......................................................285
Xinghua Pan, Alexander E. Urban, and Sherman M. Weissman
Chapter
21
Chromosome Walking by Inverse PCR
...................................................................299
Michael Gotesman, Selwyn A. Williams, Jorge
A. Garcés,
and Ray H. Gavin
Chapter
22
Expression Profiling of MicroRNAs by Quantitative
Real-Time PCR:
The Good, the Bad, and
lhe
Ugly
.............................................................................307
Mirto Castaidi, Paul
Collier,
Tania
Nolan, and Vladimir Benes
Chapter
23
Dye-Based High-Throughput qPCR in Microlluidic Platform BioMark™
.............323
David
S vec,
Vendula
Rusnakova,
Vlasta Korenkova,
and
Mikael
Kubista
Chapter
24
Surface Plasmon Resonance-Based Biosensor Technology for Real-Time
Detection of PCR Products
......................................................................................339
Giordana Feriotto
and Roberto Gambari
Chapter
25
Current Innovations in the Development and Application of qPCR
in a Resource-Limited Setting
.................................................................................355
Clare Watt and Jim Huggett
Chapter
26
A Review of Isothermal Nucleic Acid Amplification Technologies
........................363
Gavin J. Nixon and Claire A.
Bushell
Chapter
27
Proximity Ligation Assay for Protein Quantification
..............................................393
David W. Ruff, Mark E. Shannon, Shiaw-Min Chen, Simon
Fredriksson,
and
Ulf Landegren
Chapter
28
High-Resolution Melt Analysis
................................................................................409
Einar
S.
Berg and
Tania
Nolan
Index
..............................................................................................................................................423
|
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