Mucins: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2012
|
| Schriftenreihe: | Methods in molecular biology
842 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XI, 329 S. Ill., graph. Darst. |
| ISBN: | 1617795127 9781617795121 |
Internformat
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| 245 | 1 | 0 | |a Mucins |b methods and protocols |c ed. by Michael A. McGuckin ; David J. Thornton |
| 264 | 1 | |a New York [u.a.] |b Humana Press |c 2012 | |
| 300 | |a XI, 329 S. |b Ill., graph. Darst. | ||
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-024775014 | ||
Datensatz im Suchindex
| _version_ | 1804148874905911296 |
|---|---|
| adam_text | Titel: Mucins
Autor: McGuckin, Michael A.
Jahr: 2012
Contents
Preface.......................................................... v
Contributors...................................................... ix
1 Mucin Methods: Genes Encoding Mucins and Their Genetic
Variation with a Focus on Gel-Forming Mucins........................ 1
Karine Rousseau and Dallas M. Swallow
2 Gel-Forming and Cell-Associated Mucins: Preparation
for Structural and Functional Studies................................ 27
Julia R. Davies, Claes Wickström, and David J. Thornton
3 Detecting, Visualising, and Quantifying Mucins....................... 49
Cert A. Harrop, David J. Thornton, and Michael A. McGuckin
4 Mass Spectrometric Analysis of Mucin Core Proteins.................... 67
Mehmet Kesimer and John K. Sheehan
5 O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation
and Site Prediction Based on Random Peptide Substrates................ 81
Thomas A. Gerken
6 Analysis of Assembly of Secreted Mucins............................. 109
Malin E.V.Johansson and Gunnar C. Hansson
7 MUC1 Membrane Trafficking: Protocols for Assessing
Biosynthetic Delivery, Endocytosis, Recycling, and Release
Through Exosomes............................................. 123
Franz-Georg Hanisch, Carol L. Kinlough, Simon Staubach,
and Rebecca P. Hughey
8 Glycomic Work-Flow for Analysis of Mucin OLinked
Oligosaccharides............................................... 141
Catherine A. Hayes, Szilard Nemes, Samah Issa, Chunsheng Jin,
and Niclas G. Karlsson
9 O-Glycomics: Profiling and Structural Analysis of Mucin-type
O-linked Glycans.............................................. 165
Isabelle Breloy
10 O-Glycoproteomics: Site-Specific O-Glycoprotein Analysis
by CID/ETD Electrospray Ionization Tandem Mass Spectrometry
and Top-Down Glycoprotein Sequencing by In-Source Decay
MALDI Mass Spectrometry...................................... 179
Franz-Georg Hanisch
11 Analysing Mucin Degradation..................................... 191
Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline M. Rudd,
Elizabeth Matthews, and Anthony P. Corfield
VII
viii Contents
12 Assessment of Mucus Thickness and Production In Situ................. 217
Lena Holm and Mia Phillipson
13 Preservation of Mucus in Histological Sections,
Immunostaining of Mucins in Fixed Tissue, and Localization
of Bacteria with FISH........................................... 229
Malin E.V.Johansson and Gunnar C. Hansson
14 Ex Vivo Measurements of Mucus Secretion by Colon Expiants............ 237
Jenny K. Gustafison, Henrik Sjövall, and Gunnar C. Hansson
15 Establishment of Respiratory Air-Liquid Interface Cultures
and Their Use in Studying Mucin Production, Secretion,
and Function................................................. 245
David B. Hill and Brian Button
16 Studying Mucin Secretion from Human Bronchial Epithelial
Cell Primary Cultures........................................... 259
Lubna H. Abdullah, Cédric Wolber, Mehmet Kesimer,
John K. Sheehan, and C. William Davis
17 Assessment of Intracellular Mucin Content In Vivo..................... 279
Lucia Piccotti, Burton F. Dickey, and Christopher M. Evans
18 Techniques for Assessment of Interactions of Mucins with Microbes
and Parasites In Vitro and In Vivo.................................. 297
TongH. Sheng, SumairaZ. Hasnain, Chin Wen Png,
Michael A. McGuckin, and Sara K. Linden
19 Assessing Mucin Expression and Function in Human Ocular
Surface Epithelia In Vivo and In Vitro............................... 313
Pablo Argiieso and Bene K. Gipson
Index........................................................... 327
|
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| dewey-ones | 612 - Human physiology |
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| dewey-search | 612/.01575 |
| dewey-sort | 3612 41575 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Chemie Medizin |
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| id | DE-604.BV039916438 |
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| indexdate | 2024-07-10T00:14:04Z |
| institution | BVB |
| isbn | 1617795127 9781617795121 |
| language | English |
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| physical | XI, 329 S. Ill., graph. Darst. |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Mucins methods and protocols ed. by Michael A. McGuckin ; David J. Thornton New York [u.a.] Humana Press 2012 XI, 329 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 842 Springer protocols mucins cabt molecular biology cabt mucosa cabt Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Mucine (DE-588)4291070-5 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mucine (DE-588)4291070-5 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s b DE-604 McGuckin, Michael A. Sonstige oth Thornton, David J. Sonstige oth Erscheint auch als Online-Ausgabe 978-1-61779-513-8 Methods in molecular biology 842 (DE-604)BV035362695 842 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024775014&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mucins methods and protocols Methods in molecular biology mucins cabt molecular biology cabt mucosa cabt Molekularbiologie (DE-588)4039983-7 gnd Mucine (DE-588)4291070-5 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4291070-5 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Mucins methods and protocols |
| title_auth | Mucins methods and protocols |
| title_exact_search | Mucins methods and protocols |
| title_full | Mucins methods and protocols ed. by Michael A. McGuckin ; David J. Thornton |
| title_fullStr | Mucins methods and protocols ed. by Michael A. McGuckin ; David J. Thornton |
| title_full_unstemmed | Mucins methods and protocols ed. by Michael A. McGuckin ; David J. Thornton |
| title_short | Mucins |
| title_sort | mucins methods and protocols |
| title_sub | methods and protocols |
| topic | mucins cabt molecular biology cabt mucosa cabt Molekularbiologie (DE-588)4039983-7 gnd Mucine (DE-588)4291070-5 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | mucins molecular biology mucosa Molekularbiologie Mucine Methode Aufsatzsammlung |
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