Chromatin remodeling: methods and protocols
Gespeichert in:
Format: | Buch |
---|---|
Sprache: | English |
Veröffentlicht: |
New York [u.a.]
Humana Press
2012
|
Schriftenreihe: | Methods in molecular biology
833 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | Includes bibliographical references and index |
Beschreibung: | XII, 453 S. Ill., graph. Darst. 26 cm |
ISBN: | 1617794767 |
Internformat
MARC
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300 | |a XII, 453 S. |b Ill., graph. Darst. |c 26 cm | ||
336 | |b txt |2 rdacontent | ||
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490 | 1 | |a Methods in molecular biology |v 833 | |
490 | 0 | |a Springer protocols | |
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Datensatz im Suchindex
_version_ | 1804148846548221952 |
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adam_text | Titel: Chromatin remodeling
Autor: Morse, Randall H.
Jahr: 2012
Contents
Preface.............................................................. v
Contributors.......................................................... ix
1 Strain Construction and Screening Methods for a Yeast Histone H3/H4
Mutant Library.................................................... 1
Junbiao Dai andJefD. Boeke
2 Measuring Dynamic Changes in Histone Modifications and Nucleosome
Density during Activated Transcription in Budding Yeast.................... 15
Chhabi K. Govind, Daniel Ginsburg, and Alan G. Hinnebusch
3 Monitoring the Effects of Chromatin Remodelers on Long-Range
Interactions In Vivo................................................ 29
Christine M. Kiefer and Ann Dean
4 Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease........... 47
Gene O. Bryant
5 Analysis of Nucleosome Positioning Using a Nucleosome-Scanning Assay........ 63
Juan Jose Infante, G. Lynn Law, and Elton T. Young
6 Assaying Chromatin Structure and Remodeling by Restriction Enzyme
Accessibility...................................................... 89
Kevin W. Trotter and Trevor K. Archer
7 Generation of DNA Circles in Yeast by Inducible Site-Specific
Recombination.................................................... 103
MarcR. Gartenberg
8 An Efficient Purification System for Native Minichromosome
from Saccharomyces cerevisiae......................................... 115
Ashwin Unnikrishnan, Bungo Akiyoshi, Sue Biggins, and Toshio Tsukiyama
9 Simultaneous Single-Molecule Detection of Endogenous C-5 DNA
Methylation and Chromatin Accessibility Using MAPit...................... 125
Russell P. Darst, Carolina E. Pardo, Santhi Pondugula,
Vamsi K. Gangaraju, Nancy H. Nabilsi, Blaine Bartholomew,
and Michael P. Kladde
10 Analysis of Stable and Transient Protein-Protein Interactions................. 143
Stephanie Byrum, Sherri K. Smart, Signe Larson, and Alan J. Tackett
11 Monitoring Dynamic Binding of Chromatin Proteins In Vivo
by Fluorescence Recovery After Photobleaching........................... 153
Florian Mueller, Tatiana S. Karpova, Davide Mazza,
and James G. McNally
12 Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence
Correlation Spectroscopy and Temporal Image Correlation Spectroscopy........ 177
Davide Mazza, Timothy J. Stasevich, Tatiana S. Karpova,
and James G. McNally
VII
viii Contents
13 Analysis of Chromatin Structure in Plant Cells............................ 201
Mala Singh, Amol Ranjan, Krishan Mohan Rat, Sunil Kumar Singh,
Verandra Kumar, Ha Trivedi, NirajLodhi, and Samir V. Sawant
14 Analysis of Histones and Histone Variants in Plants......................... 225
Ila Trivedi, Krishan Mohan Rai, Sunil Kumar Singh,
Verandra Kumar, Mala Singh, Amol Ranjan, NirajLodhi,
and Samir V. Sawant
15 Reconstitution of Modified Chromatin Templates for In Vitro
Functional Assays.................................................. 237
Miyong Tun, Chun Ruan,Jae-Wan Huh, and Bing Li
16 A Denned In Vitro System to Study ATP-Dependent Remodeling
of Short Chromatin Fibers........................................... 255
Verena K. Maier and Peter B. Becker
17 In Vitro Reconstitution of In Vivo-Like Nucleosome Positioning
on Yeast DNA.................................................... 271
Christian J. Wippo and Philipp Korber
18 Activator-Dependent Acetylation of Chromatin Model Systems................ 289
Heather J. Szerlong and Jeffrey C. Hansen
19 Mapping Assembly Favored and Remodeled Nucleosome Positions
on Polynucleosomal Templates........................................ 311
Hillel I. Sims, ChuongD. Pham, and Gavin R. Schnitzler
20 Analysis of Changes in Nucleosome Conformation Using Fluorescence
Resonance Energy Transfer........................................... 337
Tina Shahian and GeetaJ. Narlikar
21 Preparation of Nucleosomes Containing a Specific H2A-H2A Cross-Link
Forming a DNA-Constraining Loop Structure............................ 351
Ning Liu and Jeffrey J. Hayes
22 Sulfyhydryl-Reactive Site-Directed Cross-Linking as a Method for Probing
the Tetrameric Structure of Histones H3 and H4.......................... 373
Andrew Bowman and Tom Owen-Hughes
23 Genomic Approaches for Determining Nucleosome Occupancy in Yeast......... 389
Kyle Tsui, Tanja Durbic, Marinella Gebbia, and Corey Nislow
24 Genome-Wide Approaches to Determining Nucleosome Occupancy
in Metazoans Using MNase-Seq....................................... 413
Kairong Cui and Keji Zhao
25 Salt Fractionation of Nucleosomes for Genome-Wide Profiling................ 421
Sheila S. Teves and Steven Henikoff
26 Quantitative Analysis of Genome-Wide Chromatin Remodeling............... 433
Songjoon Back, Myong-Hee Sung, and Gordon L. Hager
27 Computational Analysis of Nucleosome Positioning........................ 443
Itay Tirosh
Index............................................................... 452
|
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id | DE-604.BV039895794 |
illustrated | Illustrated |
indexdate | 2024-07-10T00:13:37Z |
institution | BVB |
isbn | 1617794767 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024754749 |
oclc_num | 756282478 |
open_access_boolean | |
owner | DE-12 DE-11 DE-355 DE-BY-UBR |
owner_facet | DE-12 DE-11 DE-355 DE-BY-UBR |
physical | XII, 453 S. Ill., graph. Darst. 26 cm |
publishDate | 2012 |
publishDateSearch | 2012 |
publishDateSort | 2012 |
publisher | Humana Press |
record_format | marc |
series | Methods in molecular biology |
series2 | Methods in molecular biology Springer protocols |
spelling | Chromatin remodeling methods and protocols ed. by Randall H. Morse New York [u.a.] Humana Press 2012 XII, 453 S. Ill., graph. Darst. 26 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 833 Springer protocols Includes bibliographical references and index Chromatin / Structure Morse, Randall H. Sonstige oth Methods in molecular biology 833 (DE-604)BV035362695 833 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754749&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Chromatin remodeling methods and protocols Methods in molecular biology Chromatin / Structure |
title | Chromatin remodeling methods and protocols |
title_auth | Chromatin remodeling methods and protocols |
title_exact_search | Chromatin remodeling methods and protocols |
title_full | Chromatin remodeling methods and protocols ed. by Randall H. Morse |
title_fullStr | Chromatin remodeling methods and protocols ed. by Randall H. Morse |
title_full_unstemmed | Chromatin remodeling methods and protocols ed. by Randall H. Morse |
title_short | Chromatin remodeling |
title_sort | chromatin remodeling methods and protocols |
title_sub | methods and protocols |
topic | Chromatin / Structure |
topic_facet | Chromatin / Structure |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754749&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035362695 |
work_keys_str_mv | AT morserandallh chromatinremodelingmethodsandprotocols |