Protein electrophoresis: methods and protocols
Saved in:
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York [u.a.]
Humana Press
2012
|
| Series: | Methods in molecular biology
869 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIV, 648 S. Ill., graph. Darst. |
| ISBN: | 9781617798207 9781617798214 |
Staff View
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV039839970 | ||
| 003 | DE-604 | ||
| 005 | 20120824 | ||
| 007 | t | ||
| 008 | 120125s2012 ad|| |||| 00||| eng d | ||
| 020 | |a 9781617798207 |c hardback |9 978-1-61779-820-7 | ||
| 020 | |a 9781617798214 |9 978-1-61779-821-4 | ||
| 035 | |a (OCoLC)775127707 | ||
| 035 | |a (DE-599)BVBBV039839970 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-20 |a DE-355 |a DE-11 |a DE-12 | ||
| 084 | |a WC 3440 |0 (DE-625)148085: |2 rvk | ||
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 245 | 1 | 0 | |a Protein electrophoresis |b methods and protocols |c ed. by Biji T. Kurien ... |
| 264 | 1 | |a New York [u.a.] |b Humana Press |c 2012 | |
| 300 | |a XIV, 648 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 869 | |
| 490 | 0 | |a Springer protocols | |
| 650 | 0 | 7 | |a Elektrophorese |0 (DE-588)4014373-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Proteine |0 (DE-588)4076388-2 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Proteine |0 (DE-588)4076388-2 |D s |
| 689 | 0 | 1 | |a Elektrophorese |0 (DE-588)4014373-9 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Kurien, Biji T. |4 edt | |
| 830 | 0 | |a Methods in molecular biology |v 869 |w (DE-604)BV035362695 |9 869 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024699880&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-024699880 | ||
Record in the Search Index
| _version_ | 1804148771320233984 |
|---|---|
| adam_text | Contents
Preface
..................................................................
ρ
Contributors
.............................................................. xi
1
How It All Began: A Personal History of Gel Electrophoresis
................. 1
Oliver Smithies
2
Introduction to Protein Electrophoresis
................................. 23
Pothur R. Srinivas
3
Protein Quantification Methods to Determine Protein Concentration
Prior to Electrophoresis
............................................. 29
J.P. Dean
Goldring
4
Protein Solubilization: Attend to the Choice of Lysis Buffer
.................. 37
Mandy
Peach,
Noëlle
Marsh, and Daniel J. MacPhee
5
Native Polyacrylamide Gels
........................................... 49
Claudia
Arndt, Stefanie
Koristka,
Holger Bartsch,
and Michael
Bachmann
6
Cationic Electrophoresis
............................................. 55
Engelbert Buxbaum
7
SARCOSYL-PAGE: A New Electrophoretic Method for the Separation
and Immunological Detection of PEGylated Proteins
....................... 65
Christian
Reichet
8
Tricine-SDS-PAGE
................................................. 81
Syed R. Haider, Helen J. Reid, and Barry L. Sharp
9
Analysis of Protein Glycation Using Phenylboronatc Acrylamidc
Gel Electrophoresis
................................................. 93
Marta
P.
Pereira Morais,
John
S.
Fossey, Tony D. James,
and Jean M.H. van
den Elsen
10
Protein Electrophoresis in
Agarose
Gels for Separating High
Molecular Weight Proteins
...........................................
Ill
Marion L. Greaser and Chad M. Warren
11
Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis
... 119
Henghang Tsai and Hon-chiu Eastwood Leung
12
A Combined Free Flow Electrophoresis and DIGE Approach to Compare
Proteins in Complex Biological Samples
................................. 135
Kim T.C. Fung, Chris Cursaro, Tanya Lewanowitsch,
Leah Cosgrove, and Peter Hoffmann
13
Immunofixation Electrophoresis for Identification of Proteins
and Specific Antibodies
.............................................. 147
György Csako
14
Microchip Electrophoresis, with Respect to Profiling of
Aß Peptides
in the
Cerebrospinal
Fluid of Patients with Alzheimer s Disease
............... 173
Mohamad
Reza Mohamadi,
Romain Verpillot,
Myriam
Taverna,
Markus
Otto, and Jean-Louis Viovy
V¡¡
viii Contents
15
A Foodomics Approach: CE-MS for Comparative Metabolomics
of Colon Cancer Cells Treated with Dietary Polyphenols
.................... 185
Mustafa
Çelebier,
Clara
Ibáñez,
Carolina
Simó,
and Alejandro Cifuentes
16
Peptide
Fractionation by SDS-Frcc Polyacrylamidc Gel Electrophoresis
for Proteomic Analysis via DF-PAGE
................................... 197
Tassel Ramos, Vladimir
Besada,
and
Lila Castellanos-Serra
17
Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous
Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass
...... 205
Monica
Cubillos-Rojas, Fabiola
Amair-Pinedo,
Irantzu
Tato,
Ramon
Bartrons,
Francese
Ventura, and Jose Luis Rosa
18
SDS-PAGE for 35S Immunoprccipitation and Immunoprecipitation
Western Blotting
................................................... 215
Edward P. Trieu and Ira
N.
Targo
ff
19
Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing
............ 235
Yaser Dorri
20
Isoelectric Focusing in
Agarose Gel
for Detection of Oligoclonal Bands
in
Cerebrospinal
and Other Biological Fluids
............................. 247
György Csako
21
Native Flatbed Isoelectric Focusing for Determining Antibody
Clonotype Distribution
.............................................. 259
Biji
T.
Kurien
and
R.
Hal Scofield
22
Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF
Followed by SDS-PAGE
............................................. 267
Hiroyuki Matsumoto, Hisao
Haniu,
Biji
T.
Kurien,
and Naoka
Komori
23
One-Dimensional and Two-Dimensional Immobilized Metal
Affinity Electrophoresis
.............................................. 275
Bao-Shiang Lee, G.D. Lasanthi, P. Jayathilaka, Jin-Sheng Huang,
and Shalini Gupta
24
Two-Dimensional Difference Gel Electrophoresis
.......................... 287
Jonathan S.
Minden
25
Protein Identification on Archived 2-D Gels
.............................. 305
Hiroyuki Matsumoto, Nobuaki Takemori, and Naoka
Komori
26
Diagonal Electrophoresis for the Detection of Protein Disulfides
............... 309
Brian McDonagh
27
Two-Dimensional Blue Native/SDS Gel Electrophoresis
of Multiprotein Complexes
........................................... 317
Jean Paul Lasserre and
Armelle
Ménard
28
Immiinoelectrophoresis: A Method with Many Faces
....................... 339
György
Csako
29
Cell Surface Protein Biotinylation for SDS-PAGE Analysis
................... 361
Giuliano Elia
30
A Multichannel Gel Electrophoresis and Continuous Fraction Collection
Apparatus for High-Throughput Protein Separation and Characterization
........ 373
Ming Dong, Megan Choi, Mark D. Biggin, and
Jian
Jin
Contents ix
31
Gel-Absorption-Based
Sample
Preparation Method for Shotgun
Analysis of Membrane Proteome
....................................... 385
Xianchun Wang and Songping Liang
32
Interactions of Hemoglobin in Live Red Blood Cells Measured
by the Electrophoresis Release Test
..................................... 393
Tan
Su, Lijun
Gao,
and Wenbin Qin
33
Extraction of Proteins from Gels: A Brief Review
.......................... 403
Biji
T.
Kurien
and
R.
Hal Scofield
34
A New Algorithm for the Simulation of
SDS 2D-PAGE Datasets..............
407
Emilio
Marengo and Elisa Robotti
35
Isolation of Proteins from Polyacrylamide Gels
............................ 427
Irene Michalk,
Stefanie Koristka,
Claudia
Arndt,
and Michael
Bachmann
36
Gel Drying Methods
................................................ 433
Slava Stamova,
Irene Michalk,
Holger
Bartsch,
and Michael
Bachmann
37
Long-Term, Buffer-Less, Wet Gel Storage in Non-scaled Polyethylene Bags
...... 437
Biji
T.
Kurien
and
R.
Hal Scofield
38
A Brief Review of Other Notable Electrophoretic Methods
................... 445
James D. Fesmire
39
Protein Stains and Applications
........................................ 451
Ranjini
K. Sundaram, Natarajan
Balasubramaniyan,
and Pazhani
Sundaram
40
Coomassie-Brilliant Blue Staining of Polyacrylamide Gels
.................... 465
Claudia
Arndt, Stefanie
Koristka,
Anja Feldmann, Holger Bartsch,
and Michael
Bachmann
41
Accelerated Coomassie Blue Staining and Destaining of
SDS-
PAGE Gels
with Application of Heat
............................................. 471
Biji
T.
Kurien
and
R.
Hal Scofield
42
Silver Staining Techniques of Polyacrylamide Gels
.......................... 481
Holger Bartsch,
Claudia
Arndt, Stefanie
Koristka,
Marc Cartellieri,
and Michael
Bachmann
43
Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide
Gel Electrophoresis
................................................. 487
Han-Min Chen
44
Counterion Dye Staining of Proteins in One- and Two-Dimensional
Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic
Gel Digestion of Stained Protein for Mass Spectrometry
..................... 497
Wei-Tao Cong, Xu Wang, Sun-Young Hwang, Li-Taijin, andJung-Kap Choi
45
TEMED-Enhanced
Photoluminescent
Imaging of Human Serum
Proteins by Quantum Dots After PAGE
................................. 511
Na Na
and Jin Ouyang
46
Microwave-Assisted Protein Staining, Destaining, and In-Gel/In-Solution
Digestion of Proteins
............................................... 521
Jennie R.
Lill
and Victor J. Nesatyy
47
Electrophoretic Separation and Detection of Mctalloproteins
by
Х
-Ray Fluorescence Mapping
....................................... 533
Tripti Khare, Yasmin Chishti, and
Lydia
A. Finney
χ
Contents
48
Fluorescent
Staining of Gels
.......................................... 543
Engelbert Buxbaum
49
A Single-Step Simultaneous Protein Staining
Procedure for Polyacrylamide Gels and Nitrocellulose
Membranes by
Alta
During Western Blot Analysis
.......................... 551
Jayanta K. Pal, Sunil K. Berwal, and
Kupali
N.
Soni
50
Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q
Emerald
300
Dye, a Fluorescent
Periodate
Schiff-Base
Stain
.................. 561
Padmaja
Mehta-D souza
51
Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly
Protein Stain
...................................................... 567
Biß
T.
Kurien,
Yaser Dorri, and R. Hal Scofield
52
In-Gel Staining of Proteins in Native Polyacrylamide Gel Electrophoresis
Using Meso-Tetrakis(4-Sulfonatophenyl)
Porphyrin........................ 579
K. Divakar,
G. Nandhini
Devi,
and Pennathur Gautam
53
Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels
with Direct Red
81
and
Amido
Black
................................... 585
David Choveaux, Robert
G.E. Krause, andJ.P. Dean Goldring
54
Zymographic Assay of
Oxidases
Using Peroxidase
or Hemin Entrapped in Polyacrylamide Gel
.............................. 591
Mircea
Alexandru
Mateescu, Carmen Calinescu, Tien Canh
Le,
Rodolfo Federico,
and Bruno
Mondovi
55
Coomassie Brilliant Blue Removal/Disposal from Gel Destain
and Used Gel Stain in an Environment-Friendly Manner
..................... 607
Biji
T,
Kurien
and Taser Dorri
56
A Brief Review of Other Notable Protein Detection Methods
on Acrylamide Gels
................................................. 617
Biji
T.
Kurien
and
R.
Hal Scofield
57
Ultrasensitive Protein Detection and Imaging: Comparison of Lumitein™,
ProteoSilver™, SYPRO® Ruby, and Coomassie® Brilliant Blue Gel Stains
......... 621
Guang Ren, Carl K. Okerberg, and Suresh T. Mathews
58
Common Artifacts and Mistakes Made in Electrophoresis
.................... 633
Biji
T.
Kurien
and
R.
Hal Scofield
Index
.................................................................. 641
|
| any_adam_object | 1 |
| author2 | Kurien, Biji T. |
| author2_role | edt |
| author2_variant | b t k bt btk |
| author_facet | Kurien, Biji T. |
| building | Verbundindex |
| bvnumber | BV039839970 |
| classification_rvk | WC 3440 WC 4150 |
| ctrlnum | (OCoLC)775127707 (DE-599)BVBBV039839970 |
| discipline | Biologie |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01636nam a2200409 cb4500</leader><controlfield tag="001">BV039839970</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20120824 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">120125s2012 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781617798207</subfield><subfield code="c">hardback</subfield><subfield code="9">978-1-61779-820-7</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781617798214</subfield><subfield code="9">978-1-61779-821-4</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)775127707</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV039839970</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-20</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-12</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 3440</subfield><subfield code="0">(DE-625)148085:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Protein electrophoresis</subfield><subfield code="b">methods and protocols</subfield><subfield code="c">ed. by Biji T. Kurien ...</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York [u.a.]</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XIV, 648 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in molecular biology</subfield><subfield code="v">869</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Springer protocols</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Elektrophorese</subfield><subfield code="0">(DE-588)4014373-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Proteine</subfield><subfield code="0">(DE-588)4076388-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Proteine</subfield><subfield code="0">(DE-588)4076388-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Elektrophorese</subfield><subfield code="0">(DE-588)4014373-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kurien, Biji T.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in molecular biology</subfield><subfield code="v">869</subfield><subfield code="w">(DE-604)BV035362695</subfield><subfield code="9">869</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024699880&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-024699880</subfield></datafield></record></collection> |
| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV039839970 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:12:26Z |
| institution | BVB |
| isbn | 9781617798207 9781617798214 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024699880 |
| oclc_num | 775127707 |
| open_access_boolean | |
| owner | DE-20 DE-355 DE-BY-UBR DE-11 DE-12 |
| owner_facet | DE-20 DE-355 DE-BY-UBR DE-11 DE-12 |
| physical | XIV, 648 S. Ill., graph. Darst. |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Protein electrophoresis methods and protocols ed. by Biji T. Kurien ... New York [u.a.] Humana Press 2012 XIV, 648 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 869 Springer protocols Elektrophorese (DE-588)4014373-9 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Elektrophorese (DE-588)4014373-9 s DE-604 Kurien, Biji T. edt Methods in molecular biology 869 (DE-604)BV035362695 869 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024699880&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein electrophoresis methods and protocols Methods in molecular biology Elektrophorese (DE-588)4014373-9 gnd Proteine (DE-588)4076388-2 gnd |
| subject_GND | (DE-588)4014373-9 (DE-588)4076388-2 (DE-588)4143413-4 |
| title | Protein electrophoresis methods and protocols |
| title_auth | Protein electrophoresis methods and protocols |
| title_exact_search | Protein electrophoresis methods and protocols |
| title_full | Protein electrophoresis methods and protocols ed. by Biji T. Kurien ... |
| title_fullStr | Protein electrophoresis methods and protocols ed. by Biji T. Kurien ... |
| title_full_unstemmed | Protein electrophoresis methods and protocols ed. by Biji T. Kurien ... |
| title_short | Protein electrophoresis |
| title_sort | protein electrophoresis methods and protocols |
| title_sub | methods and protocols |
| topic | Elektrophorese (DE-588)4014373-9 gnd Proteine (DE-588)4076388-2 gnd |
| topic_facet | Elektrophorese Proteine Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024699880&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT kurienbijit proteinelectrophoresismethodsandprotocols |