Caenorhabditis elegans: molecular genetics and development
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier, Academic Press
2011
|
| Edition: | 2. ed. |
| Series: | Methods in cell biology
106 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XIX, 488 S., [4] Bl. Ill., graph. Darst. |
| ISBN: | 9780125441728 |
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MARC
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Record in the Search Index
| _version_ | 1804148766676090880 |
|---|---|
| adam_text | CONTENTS
Contributors
xi
Preface
xv
Acknowledgments
xix
Part I
—
Genetics and Functional Genomics
1.
Mapping Mutations in
C. elegans
Eric J. Lambie
I. Introduction
4
II. Mutant Origination
4
III. Mutations That Can Be Propagated as
Homozygotes
5
IV Mutations That Cannot Be Propagated as
Homozygotes
10
V Modifier Mutations
11
VI.
SNP
Mapping
14
VII. WGS
and Beyond
18
Acknowledgments
20
References
20
2.
Specialized Chromosomes and Their Uses in Caenorhabditis
elegans
Martin R. Jones,
Zoe
Lohn
and Ann M. Rose
I. Introduction
24
II. Chromosomal Rearrangements
30
III. Characterization of Chromosomal Rearrangements
43
IV Engineered Constructs: Transgenic Arrays
48
Acknowledgements
57
References
57
Glossary
63
3.
Genome Engineering by Transgene-Instructed Gene Conversion in
C. elegans
Valerie J. P. Robert and Jean-Louis Bessereau
I. Introduction
66
II. General Features of the
МоіТІС
Technique and Experimental Outline
70
III. Methods
72
IV Materials
83
V Discussion: MosJIC Applications and Derivatives
84
VI. Summary
85
Acknowledgment
86
References
sa
Contents
4. RNAi
Methods and Screening: RNAi Based High-Throughput Genetic
Interaction Screening
Patricia G. Cipriani and
Fabio
Piano
I. Introduction: Large-Scale RNAi Screening in
C. elegans
90
II. Methods
92
III. Materials
107
Acknowledgments
109
References
109
5.
Dissection of Genetic Pathways in
C. elegans
Zheng Wang and David R. Sherwood
I. Introduction
114
II. Strategies for Identifying Components in Pathways of Interest
115
III. Ordering Genes into Pathways
140
IV. Future Outlook
148
Acknowledgments
148
References
148
Part II
—
Molecular Biology and Biochemistry
6. Transgenesis in
С
elegans
Vida
Praitis and Morris F.
Maduro
I. Introduction
162
II. Uses for
Transgenes
in
C. elegans
164
III. Construction of
Transgenes
175
IV. Obtaining Transgenic Animals
176
V Perspectives: What Lies on the Horizon?
180
VI. Summary
181
References
181
7.
RNA
Processing in
C. elegans
J.
Jason Morion and Thomas
Blumenthal
I. Introduction
188
II.
RNA
Processing in Other Organisms
188
III. RNA
Processing in
C elegans
191
Acknowledgements
211
References
211
8.
Analysis of microRNA Expression and Function
Priscilla M. Van Wynsberghe, Shih-Peng Chan, Frank J. Slack and Amy E. Pasquinelli
I. Introduction
220
II. Rationale, Methods, and Materials
221
III. Summary
247
Acknowledgments
247
References
248
Contents
9. In situ
Hybridization of
Embryos
with
Antisense
RNA
Probes
Gina Broitman-Maduro
and Morris
F.
Maduro
I. Introduction
254
II. Rationale
255
III. Methods
255
IV. Animal Preparation and Fixation
257
V. Hybridization and Signal Development
260
VI. Materials
261
VII.
Notes
264
VIII.
Troubleshooting Guide
266
IX. Imaging of Stained Embryos
267
X. Summary
268
Acknowledgments
269
References
269
10.
Gene-Centered Regulatory Network Mapping
AlberthaJ.M. Walkout
I. Introduction
272
II. Gene Regulatory Networks
274
III. Identifying Gene Regulatory Network Nodes
274
IV. Gene Regulatory Network Visualization and Analysis
282
V. Future Challenges
284
Acknowledgments
284
References
284
11. Affinity Purification of Protein Complexes in
C. elegans
Esther Zanin,
Julien Dumont,
RetoGassmann, Iain Cheeseman,
Paul Maddox, Shirin Bahmanyar, Ana
Carvalho,
Sherry Niessen,
John R.
Yates
HI, Karen Oegema, Arshad Desai
I. Introduction
291
II. Generating Tools for Biochemistry
292
III. The Optimal Starting Material for Protein Purification
298
IV Isolation of Nuclei from Embryos
305
V. Preparing Worm and Embryonic Extract
307
VI. Single-step Immunoprecipitation
309
VII.
Tandem Affinity Purification Using a LAP Tag
з із
VIII.
Mass Spectrometry
&
Prioritization for Follow-up
Experiments 3I4
IX. Summary
315
X. Solutions and Media
315
XI. Equipment
320
Acknowledgments
320
References
320
viii Contents
Part III
—
Development
12.
Cell Identification and Cell Lineage Analysis
Claudiu A. Giurumescu and Andrew D. Chisholm
I. Introduction
326
II. Rationale
328
III. Resources
330
IV Nomenclature and Conventions
330
V Cell Identification and Lineage Analysis
331
VI. Materials, Methods and Protocols
335
VII.
Discussion
338
Acknowledgments
338
References
338
13.
The Genetics and Cell Biology of Fertilization
Brian D. Geldziler, Mathew R.
Marcello,
Diane C. Shakes and Andrew Singson
I. General Introduction/Background
344
II. Finding Sterile Mutants
349
III. Maintaining Sterile Mutants
352
IV. Fecundity Analysis
354
V.
Ovulation/Ovulation
Rates
355
VI. Spermatogenesis
356
VII.
Sperm Migratory Behavior/Tracking
358
VIII.
Sperm Competition
359
IX.
Oogénesis
and Oocyte Maturation
360
X. Assessing Fertilization and Egg Activation in Egg-Sterile Mutants
361
XI. Eggshell Production
365
XII.
Analysis of Fertilization-Specific Gene Products
367
XIII.
Future Prospects/Issues
368
Acknowledgments
369
References
369
14.
Imaging Embryonic Morphogenesis in
C. elegans
Jeff Hardin
I. Introduction
378
II. Methods
378
III. Summary
410
Acknowledgments
411
References
411
15.
Molecular and Genetic Approaches for the Analysis of
C. elegans
Neuronal
Development
Dong Yan and Tishi Jin
I. Introduction
414
II. Strategies for In Vivo Labeling of Neurons and Compartments
417
Ш.
Other Methods for Examining the Development of Neurons
426
Contents
IV Functional Dissection of Signaling Pathways Controlling
Neuronal
Development
427
V Examples of
Neuronal
Development
434
VI. Outlook
436
References
437
16.
Methods for Evaluating the Caenorhabditis
elegans
Dauer
State:
Standard Dauer-formation Assay Using Synthetic Daumones and Proteomic
Analysis of O-GlcNAc Modifications
Jeeyong Lee, Kwang-Youl Kim, Hyoe-Jin Joo, Heekyeong Kim,
Pan-Young Jeong, and Young-Ki Paik
447
449
451
456
457
458
458
459
461
463
479
I.
Introduction
II.
Rationale
III.
Methods
IV
Materials
V
Discussion
VI.
Summary
Acknowledgments
References
Appendix
Index
Volumes in
Series
|
| any_adam_object | 1 |
| author_GND | (DE-588)1018861211 |
| building | Verbundindex |
| bvnumber | BV039836517 |
| classification_rvk | WC 5100 |
| ctrlnum | (OCoLC)772628307 (DE-599)BVBBV039836517 |
| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| spelling | Caenorhabditis elegans molecular genetics and development ed. by Joel H. Rothman ... 2. ed. Amsterdam [u.a.] Elsevier, Academic Press 2011 XIX, 488 S., [4] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 106 Methode (DE-588)4038971-6 gnd rswk-swf Entwicklung (DE-588)4113450-3 gnd rswk-swf Caenorhabditis elegans (DE-588)4147127-1 gnd rswk-swf Genetik (DE-588)4071711-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Caenorhabditis elegans (DE-588)4147127-1 s Genetik (DE-588)4071711-2 s Methode (DE-588)4038971-6 s DE-604 Entwicklung (DE-588)4113450-3 s b DE-604 Rothman, Joel H. Sonstige (DE-588)1018861211 oth Methods in cell biology 106 (DE-604)BV002534878 106 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024696504&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Caenorhabditis elegans molecular genetics and development Methods in cell biology Methode (DE-588)4038971-6 gnd Entwicklung (DE-588)4113450-3 gnd Caenorhabditis elegans (DE-588)4147127-1 gnd Genetik (DE-588)4071711-2 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4113450-3 (DE-588)4147127-1 (DE-588)4071711-2 (DE-588)4143413-4 |
| title | Caenorhabditis elegans molecular genetics and development |
| title_auth | Caenorhabditis elegans molecular genetics and development |
| title_exact_search | Caenorhabditis elegans molecular genetics and development |
| title_full | Caenorhabditis elegans molecular genetics and development ed. by Joel H. Rothman ... |
| title_fullStr | Caenorhabditis elegans molecular genetics and development ed. by Joel H. Rothman ... |
| title_full_unstemmed | Caenorhabditis elegans molecular genetics and development ed. by Joel H. Rothman ... |
| title_short | Caenorhabditis elegans |
| title_sort | caenorhabditis elegans molecular genetics and development |
| title_sub | molecular genetics and development |
| topic | Methode (DE-588)4038971-6 gnd Entwicklung (DE-588)4113450-3 gnd Caenorhabditis elegans (DE-588)4147127-1 gnd Genetik (DE-588)4071711-2 gnd |
| topic_facet | Methode Entwicklung Caenorhabditis elegans Genetik Aufsatzsammlung |
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