Synthesis and evaluation of redox-center modified DNA:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
München
Verl. Dr. Hut
2011
|
| Edition: | 1. Aufl. |
| Series: | Organische Chemie
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | IX, 133 S. graph. Darst. 210 mm x 148 mm, 244 g |
| ISBN: | 9783843902403 3843902402 |
Staff View
MARC
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Record in the Search Index
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|---|---|
| adam_text | IMAGE 1
TABLE OF CONTENTS
SUMMARY I
ZUSAMMENFASSUNG V
1 INTRODUCTION 1
1.1 DNA 1
1.2 DNA DAMAGE 5
1.2 DNA DAMAGE 5
1.3 DNA REPAIR 11
1.3.1 THE ROLE OF FLAVINS IN DNA REPAIR 13
1.4 ELECTRON TRANSFER PROCESSES IN DNA 14
1.4.1 TRANSFER MECHANISMS 16
1.4.2 OXIDATIVE HOLE TRANSFER 17
1.4.3 REDUCTIVE ELECTRON TRANSFER 19
1.4.3 ELECTRON ACCEPTORS 24
1.5 COORDINATION OF METAL IONS BY DNA 26
1.5.1 METAL BASE PAIRS 26
1.6 COPPER-CATALYZED CLICK CHEMISTRY 29
1.6.1 REACTION MECHANISM 31
1.6.2 COPPER-CATALYZED CLICK REACTION ON DNA 33
2 DEVELOPMENT OF A NEW CHEMICAL SYSTEM FOR THE ESTIMATION OF EET AND CPD
CLEAVA&E RATES IN DNA 35
2.1 FLAVIN PHOTOREDUCTION 37
2.2 SYNTHESIS OF THE DNA DOUBLE-DUPLEX 38
2.3 DNA DOUBLE-DUPLEX CONTAINING ONLY BR-DU 40
2.3 DNA DOUBLE-DUPLEX CONTAINING ONLY BR-DU 40
2.3.1 DATA COLLECTION AND ANALYSIS OF THE PRODUCTS 43
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1018404724
DIGITALISIERT DURCH
IMAGE 2
2.4 DOES THE SECOND ELECTRON TRAP INFLUENCE THE REACTION AT THE FIRST?
47
2.5 DOUBLE-DUPLEXES CONTAINING BR-DU, BR-DA, BR-DG AND T=T 48
2.5.1 DATA COLLECTION AND ANALYSIS OF THE PRODUCTS 50
2.6 RESULTS AND DICUSSION 52
3 FLAVIN-INDUCED DNA PHOTOOXIDATION 57
3.1 SYNTHESIS OF THE DNA HAIRPIN 59
3.2 MELTING CURVES AND CD SPECTRA 62
3.3 TRANSIENT SPECTROSCOPY OF THE HAIRPINS 63
3.3.1 DETAILS OF THE FEMTOSECOND BROADBAND PUMP-PROBE SPECTROMETER 63
3.3.2 CHARACTERISTIC SIGNALS IN THE TRANSIENT SPECTRA OF FREE FLAVIN
MOLECULES 65
3.4 RESULTS AND DISCUSSION 67
4 METAL-SALEN COMPLEXES IN THE MAJOR GROOVE OF DNA.... 73
4.1 SYNTHESIS OF THE ALKYNE MODIFIED DNA 74
4.2 CLICK REACTION OF THE ALKYNE-MODIFIED DNA WITH SALICYLIC ALDEHYDE 76
4.3 ASSEMBLY AND CHARACTERIZATION OF THE METAL-SALEN COMPLEX 78
4.3 ASSEMBLY AND CHARACTERIZATION OF THE METAL-SALEN COMPLEX 78
4.4 ESI MASS SPECTROMETRY 79
4.5 MELTING POINT INVESTIGATIONS 83
4.6 UV-VIS SPECTROSCOPIC INVESTIGATIONS 85
4.7 CRYSTALLIZATION EXPERIMENTS 88
4.8 WHERE IS THE SECOND COORDINATION SITE PLACED? 89
4.9 USE OF THE NEW MODEL SYSTEM IN CATALYSIS 91
5 EXPERIMENTAL
SECTION..........................................................................
93
5.1 MATERIALS AND METHODS 93
5.2 DNA SYNTHESIS, CLEAVAGE AND PURIFICATION 93
IMAGE 3
5.3 MASS SPECTROMETRY 95
5.4 MELTING POINT EXPERIMENTS 95
5.5 CD SPECTRA 96
5.6 IRRADIATION EXPERIMENTS 96
5.7 FEMTOSECOND TRANSFER ABSORPTION 97
5.8 UV/VIS TITRATION 97
5.9 PROCEDURE FOR CLICK REACTION 98
5.10 CRYSTALLIZATION EXPERIMENT 98
ANNEX 99
A.1 DNA SOLID PHASE SYNTHESIS PROTOCOL FOR//-PHOSPHORATES 99
A.2 HPLC CHROMATOGRAMS AND MALDI-TOF ANALYSIS 102
A.3 IRRADIATION DATA 103
A.4 DNA METAL COMPLEX: TITRATION CURVE 104
ABBREVIATIONS 105
REFERENCES 109
ACKNOWLEDGMENTS 129
CURRICULUM VITAE 131
|
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| genre_facet | Hochschulschrift |
| id | DE-604.BV039816859 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:12:06Z |
| institution | BVB |
| isbn | 9783843902403 3843902402 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024677058 |
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| owner_facet | DE-12 DE-19 DE-BY-UBM DE-29T |
| physical | IX, 133 S. graph. Darst. 210 mm x 148 mm, 244 g |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Verl. Dr. Hut |
| record_format | marc |
| series2 | Organische Chemie |
| spelling | Fazio, Danila 1980- Verfasser (DE-588)1018996656 aut Synthesis and evaluation of redox-center modified DNA Danila Fazio 1. Aufl. München Verl. Dr. Hut 2011 IX, 133 S. graph. Darst. 210 mm x 148 mm, 244 g txt rdacontent n rdamedia nc rdacarrier Organische Chemie Zugl.: München, Univ., Diss., 2011 DNS (DE-588)4070512-2 gnd rswk-swf Redoxpolymere (DE-588)4394331-7 gnd rswk-swf Funktionalisierung Chemie (DE-588)4279323-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content DNS (DE-588)4070512-2 s Funktionalisierung Chemie (DE-588)4279323-3 s Redoxpolymere (DE-588)4394331-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024677058&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fazio, Danila 1980- Synthesis and evaluation of redox-center modified DNA DNS (DE-588)4070512-2 gnd Redoxpolymere (DE-588)4394331-7 gnd Funktionalisierung Chemie (DE-588)4279323-3 gnd |
| subject_GND | (DE-588)4070512-2 (DE-588)4394331-7 (DE-588)4279323-3 (DE-588)4113937-9 |
| title | Synthesis and evaluation of redox-center modified DNA |
| title_auth | Synthesis and evaluation of redox-center modified DNA |
| title_exact_search | Synthesis and evaluation of redox-center modified DNA |
| title_full | Synthesis and evaluation of redox-center modified DNA Danila Fazio |
| title_fullStr | Synthesis and evaluation of redox-center modified DNA Danila Fazio |
| title_full_unstemmed | Synthesis and evaluation of redox-center modified DNA Danila Fazio |
| title_short | Synthesis and evaluation of redox-center modified DNA |
| title_sort | synthesis and evaluation of redox center modified dna |
| topic | DNS (DE-588)4070512-2 gnd Redoxpolymere (DE-588)4394331-7 gnd Funktionalisierung Chemie (DE-588)4279323-3 gnd |
| topic_facet | DNS Redoxpolymere Funktionalisierung Chemie Hochschulschrift |
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| work_keys_str_mv | AT faziodanila synthesisandevaluationofredoxcentermodifieddna |