Neurotrophic factors: methods and protocols
Saved in:
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York, NY [u.a.]
Humana Press, Springer
2012
|
| Series: | Methods in molecular biology
846 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Klappentext |
| Physical Description: | XIV, 421 S. Ill., graph. Darst. |
| ISBN: | 9781617795350 |
Staff View
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Record in the Search Index
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|---|---|
| adam_text | Contents
Preface
..............................................................
v
Contributors
.......................................................... xi
1
The Neurotrophin Family of Neurotrophic Factors: An Overview
.......... 1
Stephen D. Skater
2
Neuronal
Growth-Promoting and Inhibitory Cues
in
Neuroprotection
and Neuroregeneration
.......................... 13
Stephen D. Simper
3
Culture of Rat Cerebellar Granule Neurons and Application
to Identify Neuroprotective Agents
................................. 23
Laura· Facci
and Stephen D. Skaper
4
Isolation and Culture of Neural Progenitor Cells from Rat
Postnatal Cerebellum
........................................... 39
Morena
Zusso and
Patrizia Debetto
5
Culture of Rodent Cortical and Hippocampal Neurons
.................. 49
Laura
Facci
and Stephen D. Skaper
6
Amyloid
ß-Peptide Neurotoxicity
Assay Using Cultured
Rat Cortical Neurons
........................................... 57
Laura
Facci
and Stephen D. Skaper
7
Culture of Neonatal Rodent Microglia, Astrocytes,
and Oligodendrocytes from Cortex and Spinal Cord
.................... 67
Stephen D. Skaper,
Carla Argentini,
and Massimo Barbierato
8
Central Nervous System Neuron-Glia Co-culture Models
................ 79
Stephen D. Skaper and Laura
Facci
9
Culture and Characterization of Rat Mesencephalic
Dopaminergic Neurons
.......................................... 91
Stephen D. Skaper,
Giulia Mercanti,
and Laura
Facci
10
Preparation of Adult Spinal Cord Motor Neuron Cultures
Under Serum-Free Conditions
.................................... 103
Jose V. Montoya-Gacbarnaffltonjairo Sutachan,
Wai Si Chan, Alexandra Sideris, Thomas JJ.
Blanek,
and
Esperanza Recio-Pinto
11
Rodent Retinal Ganglion Cell Cultures
.............................. 117
Stephen D. Skaper
12
Culture of Purified Glial CeU Populations from Optic Nerve
.............. 131
Stephen D. Skaper
13
Isolation and Culture of Rat Cone Photoreceptor Cells
.................. 147
Stephen D. Skaper
VII
viii Contents
14
Culture
of
Rat Retina
Pigmented
Epithelial Cells
...................... 159
Stephen
D.
Skater
15
Mammalian Growth Cone Turning Assays Identify Distinct
Cell Signalling Mechanisms That Underlie Axon Growth,
Guidance and Regeneration
...................................... 167
Andrew]. Murray, Andrew G. Peace, Steven J. Tucker,
and Derryck A. Shewan
16
Culture of Dissociated Sensory Neurons from Dorsal Root
Ganglia of Postnatal and Adult Rats
................................ 179
Davina
E.
Owen and Julie Egerton
17
Culture and Proliferation of Highly Purified Adult Schwann Cells
from Rat, Dog, and Man
......................................... 189
Kirsten Haastert-Talini
18
Use of PC12 Cells and Rat Superior Cervical Ganglion
Sympathetic Neurons as Models for Neuroprotective Assays
Relevant to Parkinson s Disease
.................................... 201
Cristina
Malagelada
Grau
and Lloyd A. Greene
19
Compartmented Chambers for Studying Neurotrophic Factor Action
....... 213
Stephen D. Skaper
20
Preparation and Culture of Adrenal
Chromaffin
Cells
................... 223
Natalia
Domínguez,
Miriam
Rodríguez, J. David Machado,
and
Ricardo
Borges
21
Indirect Immunofluorescence Staining of Cultured Neural Cells
........... 235
Massimo Barbierato,
Carla Argentini,
and Stephen D. Skaper
22
Neurite Outgrowth Assessment Using High Content
Analysis Methodology
........................................... 247
Nicholas M. Radio
23
Dissociated Cell Culture for Testing Effects of Carbon Nanotubes
on
Neuronal
Growth
........................................... 261
William Lee and Vladimir Parpura
24
High-Resolution Imaging and Evaluation of Spines in Organotypic
Hippocampal Slice Cultures
...................................... 277
Frederik Sündermann, Nataliya Golovyashkina,
Christian
Tackenberg,
Roland Brandt, and
Lidia
Bakota
25
Imaging Amyloid Precursor Protein In Vivo:
An Axonal Transport Assay
....................................... 295
Tomás
L.
Fatzone
and
Gorazd B. Stokin
26
The Use of Specific AAV Serotypes to Stably Transduce Primary
CNS Neuron Cultures
.......................................... 305
Saafan Z. Malik, Margaret A.
Maronski,
Marc
Α.
Dichter,
and Deborah
J.
Watson
27
Preparation and Characterization of
Biocompatible Chitosan
Nanoparticles for Targeted Brain Delivery of Peptides
................... 321
Secil
Caban, Ttlmaz
Capan,
Patrick
Couvreur,
and Turgay Dalkara
Contents ix
28 [3H]Serotonin Release
Assay Using Antigen-Stimulated
Rat
Peritoneal Mast Cells
........................................ 333
Stephen D. Skater and
Laura Facci
29
Rat Hippocampal Slice Culture Models for the Evaluation
of Neuroprotective Agents
....................................... 343
Elisabetta Gerace,
Elisa
Landucci,
Tania
Scartabelli,
Flavio Moroni, and
Domenico E.
Pellegrini-Giampietro
30
A 6-Hydroxydopamine In Vivo Model of Parkinson s Disease
............. 355
Giulia Mercanti, Gianfranco
Bazzu, and
Pietro Giusti
31
Brain Microdialysis in Freely Moving Animals
......................... 365
Gianfranco Bazzu, Alice Biosa, Donatella Farina,
Ylenia Spissu, Giammario Calia,
Sonia
Dedola,
Gaia Rocchitta, Rossana Micheli, Pier
Andrea
Serra,
and Maria Speranza Desole
32
Evaluating
Motor
Neuron Death in Neonatal Rats Subjected
to Sciatic Nerve Lesion
.......................................... 383
Andre Scbwambach
Vieira, Alexandre
Cesar Santos
de Rezende,
and
Fabio
Rogério
33
Rodent Spinal Cord Injury Model and Application of Neurotrophic
Factors for
Neuroprotection
...................................... 393
Hart Shanker Sharma and Aruna Sharma
Index
............................................................... 417
Methods in Molecular Biology
846
John M. Walker, Series Editor
Stephen D. Skaper Editor
Neurotrophic Factors
Methods and Protocols
Nervous system development evolves from the well-orchestrated processes of neural induction,
cell proliferation, differentiation, cell migration, survival, and synapse formation. Among these
environmental cues, neurotrophic factors are secreted proteins that promote neurite outgrowth,
neuronal
cell differentiation and survival both in vivo and in vitro. Nerve growth factor (NGF)
is the founding and best characterized member of the neurotrophin family ofneurotrophic
polypeptides. Neurotrophic Factors: Methods and Protocols presents a selection of protocols and
procedures which make use of cellular, tissue, and whole animal models which can be applied to
the investigation ofneurotrophic factors and other agents impacting on these systems. Chapters
cover a wide-range of topics such as dealing with the culture ot neurons and
glia
from the central
and peripheral nervous systems, neuron-glia co-culture models, and cell-based assays for the
evaluation of neuroprotective molecules, as well as assays which can be applied to the study of
agents with neuroregenerative potential. Protocols describing viral- and nanoparticle-based de¬
livery methods to neural cells are also presented, followed by chapters dealing with organotypic
slice culture protocols. Lastly, several chapters are dedicated to in vivo lesion models of relevance
to nervous system pathology, which can be applied to the investigation ofneurotrophic factors
and peptides. Written in the successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known
pitfalls.
Authoritative and easily accessible, Neurotrophic Factors: Methods and Protocols seeks to serve
both professionals and novices alike with its well-honed methodologies in an effort to further
our knowledge of what has been described as the last frontier of science.
|
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| spelling | Neurotrophic factors methods and protocols ed. by Stephen D. Skaper New York, NY [u.a.] Humana Press, Springer 2012 XIV, 421 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 846 Springer protocols Neurotropher Faktor (DE-588)4317638-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Neurotropher Faktor (DE-588)4317638-0 s Methode (DE-588)4038971-6 s DE-604 Skaper, Stephen D. edt Erscheint auch als Online-Ausgabe 978-1-61779-536-7 Methods in molecular biology 846 (DE-604)BV035362695 846 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024498764&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024498764&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Neurotrophic factors methods and protocols Methods in molecular biology Neurotropher Faktor (DE-588)4317638-0 gnd Methode (DE-588)4038971-6 gnd |
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| title | Neurotrophic factors methods and protocols |
| title_auth | Neurotrophic factors methods and protocols |
| title_exact_search | Neurotrophic factors methods and protocols |
| title_full | Neurotrophic factors methods and protocols ed. by Stephen D. Skaper |
| title_fullStr | Neurotrophic factors methods and protocols ed. by Stephen D. Skaper |
| title_full_unstemmed | Neurotrophic factors methods and protocols ed. by Stephen D. Skaper |
| title_short | Neurotrophic factors |
| title_sort | neurotrophic factors methods and protocols |
| title_sub | methods and protocols |
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