Tag-based next generation sequencing:
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| Format: | Book |
| Language: | English |
| Published: |
Weinheim
Wiley-Blackwell
2012
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| Subjects: | |
| Online Access: | Inhaltstext Inhaltsverzeichnis |
| Item Description: | Literaturangaben |
| Physical Description: | XXV, 581 S. Ill., graph. Darst. 240 mm x 170 mm |
| ISBN: | 9783527328192 352732819X |
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Edited by
Matthias Harbers and Cunter Kahl
Tag-based Next Generation Sequencing
WILEY-BLACKWELL
Contents
Preface XIX
List of Contributors XXI
Part One Tag-Based Nucleic Acid Analysis 7
1 DeepSuperSACE: High-Throughput Transcriptome Sequencing with
Now- and Next-Generation Sequencing Technologies 3
Hideo Matsumura, Carlos Molina, Detlev H Kriiger, Ryohei Terauchi,
and Cunter Kahl
1 1 Introduction 3
1 2 Overview of the Protocols 5
121 Principle of the SuperSAGE Method 5
122 Power of the SuperSAGE Tag 5
123 Development of DeepSuperSAGE 6
124 Ditag-Based DeepSuperSAGE (for 454 Pyrosequencing) 7
125 Single-Tag-Based DeepSuperSAGE (HT-SuperSAGE) 8
1 3 Methods and Protocols 9
131 Linker or Adapter Preparation 9
132 RNA Samples 10
133 cDNA Synthesis and Nlalll Digestion 10
134 Tag Extraction from cDNA 10
135 Tag Extraction from cDNA 11
136 Purification of Linker-Tag Fragments 12
137 Ditag or Adapter-Tag Formation and Amplification 12
138 Preparation of Templates for Sequencing 14
1 4 Applications 14
141 Applications of DeepSuperSAGE in Combination with 454
Pyrosequencing 14
142 Practical Analysis of HT-SuperSAGE 18
1 5 Perspectives 19
References 20
2 DeepCACE: Genome-Wide Mapping of Transcription
Start Sites 23
Matthias Harbers, Mitchell S Dushay, and Piero Carninci
2 1 Introduction 23
2 2 What is CAGE? 24
2 3 Why CAGE? 26
2 4 Methods and Protocols 28
2A I Key Reagents and Consumables 28
242 Precautions 30
243 RNA Samples Used for DeepCAGE Library Preparation 30
244 DeepCAGE Library Preparation 32
2 5 Applications 43
2 6 Perspectives 44
References 45
3 Definition of Promotome-Transcriptome Architecture Using CAGEscan 47
Nicolas Bertin, Charles Plessy, Piero Caminci, and Matthias Harbers
3 1 Introduction 47
3 2 What is CAGEscan? 48
3 3 Why CAGEscan? 50
3 4 Methods and Protocols 51
341 Key Reagents and Consumables 51
342 Precautions 53
343 RNA Samples Used for CAGEscan Library Preparation 53
344 Considerations on Pooling CAGEscan Libraries 54
345 CAGEscan Library Preparation 54
3 5 Applications and Perspectives 59
References 61
4 RACE: New Applications of an Old Method to Connect Exons 63
Charles Plessy
4 1 Introduction 63
4 2 Deep-RACE 65
421 Choice of the Sequencer 65
422 Validation of Promoter Studies 65
423 Other Applications of Deep-RACE 66
424 Limitations of Deep-RACE 66
4 3 Methods Outline 67
431 Primer Design 67
432 Molecular Biology of Deep-RACE Library Preparation 67
433 Sequencing of Deep-RACE Libraries 68
434 Analysis 68
4 4 Perspectives 70
References 71
5 RNA-PET: Full-Length Transcript Analysis Using 5'- and 3'-Paired-End Tag
Next-Generation Sequencing 73
Xiaoan Ruan and Yijun Ruan
5 1 Introduction 73
5 2 Methods and Protocols 75
521 Key Reagents and Consumables 75
522 Protocol 78
5 3 Applications 88
531 PET Sequencing with SOLiD 88
532 Mapping of the PETs 88
533 PET Clustering, Annotation, and Genome Browser Visualization 89
5 4 Perspectives 90
References 90
6 Stranded RNA-Seq: Strand-Specific Shotgun Sequencing of RNA 91
Alistair R R Forrest
6 1 Introduction 91
611 Before Starting 93
6 2 Methods and Protocols 93
621 Preface 93
622 Materials and Consumables 94
Contt
623 Protocol 95
6 3 Bioinformatic Considerations 103
6 4 Applications 104
6 5 Perspectives 105
References 107
7 Differential RNA Sequencing (dRNA-Seq): Deep-Sequencing-Based Analysis
of Primary Transcriptomes 709
Anne Borries, J'brg Vogel, and Cynthia M Sharma
7 1 Introduction 109
7 2 What is dRNA-Seq? Ill
7 3 Why dRNA-Seq? 112
7 4 Methods and Protocols 115
741 Materials and Consumables 115
742 Precautions 116
7 A3 RNA Samples Used for dRNA-Seq Library Preparation 116
744 dRNA-Seq Library Preparation 116
7 5 Applications 119
7 6 Perspectives 120
References 121
8 Identification and Expression Profiling of Small RNA Populations Using
High-Throughput Sequencing 123
Javier Armisen, W Robert Shaw, and Eric A Miska
8 1 Introduction 123
811 miRNAs 123
812 piRNAs 125
813 siRNAs 126
814 Other Small RNAs 126
8 2 HTS/NGS 127
8 3 Methods and Protocols 128
831 Key Reagents and Solutions 128
832' Total RNA Isolation 129
833 Small RNA Isolation 129
834 Ligation of Adapters 131
8 4 Troubleshooting 134
8 5 Applications 134
8 6 Perspectives 136
References 138
9 Genome-Wide Mapping of Protein-DNA Interactions by ChlP-Seq 139
Joshua W K Ho, Artyom A Alekseyenko, Mitzi I Kuroda, and PeterJ Park
9 1 Introduction 139
9 2 Methods and Protocols 141
921 Antibody Validation 141
922 ChIP 141
923 Sequencing Library Preparation 144
924 Data Analysis 146
9 3 Applications 147
931 Deciphering the Transcriptional Regulatory Program 148,
932 Unraveling Epigenetic Regulation 148
933 Comparative Interindividual or Interspecies Analysis 149
934 Study of Human Diseases and Clinical Applications 149
935 Advantages and Challenges of ChlP-Seq 149
9 4 Perspectives 150
References 151
10 Analysis of Protein-RNA Interactions with Single-Nucleotide Resolution
Using iCLIP and Next-Generation Sequencing 753
Julian Kbnig, Nicholas J McClincy, andjernej Ule
10 1 Introduction 153
10 2 Procedure Overview 154
10 3 Antibody and Library Preparation Quality Controls 155
10 4 Oligonucleotide Design 156
10 5 Recent Modifications of the iCLIP Protocol 158
10 6 Troubleshooting 158
10 7 Methods and Protocols 159
References 169
11 Massively Parallel Tag Sequencing Unveils the Complexity of Marine
Protistan Communities in Oxygen-Depleted Habitats 777
Virginia Edgcomb and Thorsten Stoeck
11 1 Introduction 171
11 2 Cariaco Basin 173
11 3 Framvaren Fjord 176
11A Comparison of Cariaco Basin to Framvaren Fjord 177
11 5 Perspectives on Interpretation of Microbial Eukaryote 454 Data 179
References 182
12 Chromatin Interaction Analysis Using Paired-End Tag Sequencing
(ChlA-PET) 185
Xiaoan Ruan and Yijun Ruan
12 1 Introduction 185
12 1 1 Development of the ChlA-PET Method 186
12 1 2 Applications of the ChlA-PET Method 187
12 1 3 Experimental Design of ChlA-PET Analysis 187
12 131 ChIP Sample Preparation 187
12 132 ChlA-PET Library Construction 189
12 133 ChlA-PET Library Sequencing and Mapping 190
12 134 Control Libraries 191
12 2 Methods and Protocols 192
12 2 1 Key Reagents and Consumables 192
12 2 2 Protocol 195
12 3 Timeline 206
12 A Anticipated Results 207
12 A I Verification of Sonicated Chromatin DNA Size Range 207
12 4 2 ChIP Quality Control: Yield and Enrichment 207
12 4 3 ChlA-PET Library Quality Control 207
12 4 4 ChlA-PET Sequencing and Mapping Analysis 207
12 5 Perspectives 209
References 209
13 Tag-Seq: Next-Generation Tag Sequencing for Gene Expression
Profiling 27 7
Sorana Morrissy, Yongjun Zhao, Allen Delaney, Jennifer Asano, Noreen Dhalla,
Irene Li, Helen McDonald, Pawan Pandoh, Anna-Liisa Prabhu, Angela Tarn,
Martin Hirst, and Marco Marra
13 1 Introduction 211
13 2 Protocol Details 212
13 3 Protocol Overview and Timeline 213
13 4 Critical Parameters and Troubleshooting 214
13 5 Methods and Protocols 215
13 5 1 Basic Protocol 1: First- and Second-Strand cDNA Synthesis for Tag-Seq
Library Construction 215
13 5 2 Basic Protocol 2: Tag Generation 219
13 5 3 Basic Protocol 3: PCR and Fragment Isolation 223
13 5 4 Basic Protocol 4: Preparing the Library for Illumina Sequencing 226
13 5 5 Alternate Protocol: Amplified Tag-Seq library construction
(Tag-SeqLite) 227
13 5 6 Basic Protocol 5: Data Analysis 232
13 6 Applications 239
13 7 Perspectives 240
References 241
14 Isolation of Active Regulatory Elements from Eukaryotic Chromatin Using
FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements) 243
Paul C Ciresi and Jason D Lieb
14 1 Introduction 243
14 2 Methods and Protocols 245
14 2 1 FAIRE Procedure 245
14 2 2 Optimization of the FAIRE Procedure 245
14 2 3 Equipment and Reagents 246
14 2 4 Detection of FAIRE DNA 250
14 2 5 High-Throughput Sequencing 252
14 3 Applications 254
14 4 Perspectives 254
References 255
15 Identification of Nucleotide Variation in Genomes Using Next-Generation
Sequencing 257
Hendrik-Jan Megens and Martien A M Croenen
15 1 Introduction 257
15 1 1 SNP Discovery and Nucleotide Variation Assessment 259
15 1 2 Sequence and Library Preparation Strategies 259
15 2 Methods 261
15 2 1 Preprocessing of Reads 262
15 211 FASTQ Format 262
15 212 FASTQ Format - Illumina Version 262
15 213 Illumina FASTQ to Sanger FASTQ 263
15 214 ABI SOLiD- and Roche 454-Specific Formats 264
15 215 Illumina SCARF or QSEQ to FASTQ 264
15 216 Quality Evaluation 265
15 217 Handling Adapter Sequences - Linkers and Barcodes 265
15 218 Quality Trimming 266
15 2 2 Mapping Reads to a Reference Genome 266
15 221 Making Alignments Using MOSAIK 267
15 222 Making Alignments Using BWA 268
15 2 3 Variant Calling 269
15 231 SAM Format 270
15 232 Variant Calling with SAMtools -273
15 233 Variant Calling with GATK 274
15 3 Notes 275
References 275
16 DGS (Ditag Genome Scanning) - A Restriction-Based Paired-End
Sequencing Approach for Genome Structural Analysis 277
Jun Chen, Yeong C Kim, and San Ming Wang
16 1 Introduction 277
16 2 Methods and Protocols 278
16 2 1 Cloning-Based DGS Protocol 278
16 2 2 Non-Cloning-Based DGS Protocol 281
16 2 3 Computational Mapping Analysis of Experimental Ditags 282
16 3 Applications 283
16 3 1 Analyzing Normal Genome Structure 283
16 3 2 Identifying Somatic Rearrangements in Cancer Genomes 283
16 33A Useful Tool to Study Family Germline Genetic Disorders 284
16 4 Perspectives 284
References 285
17 Next-Generation Sequencing of Bacterial Artificial Chromosome Clones
for Next-Generation Physical Mapping 287
Robert Bogden, Keith Stormo, Jason Dobry, Amy Mraz, Quanzhou Tao, Michiel
van Eijkjan van Oeveren, Marcel Prims, Jon Wittendorp, and Mark van Haaren
17 1 History of the Bacterial Artificial Chromosome Vector Systems 287
17 2 History of Physical Mapping 288
17 3 What is WGP? 289
17 A Flow of a WGP Project 289
17 5 BAC Pooling Strategies 290
17 6 Methods and Protocols 291
17 6 1 BAC Library and Pooling Strategy 291
17 6 2 Sample Preparation for Illumina Sequencing 292
17 6 3 Illumina Sequencing 293
17 6A Deconvolution to Assign the BAC Address to Each Read 293
17 6 5 Contig Building 293
17 7 Applications 294
17 7 1 Results from Real WGP Projects Performed by the Authors 294
17 7 2 Reorganizing Project Funding and Sequencing Budgets 295
17 7 3 Unleash the Power of BAC Clones 296
17 8 Perspectives 296
References 297
18 HELP-Tagging: Tag-Based Genome-Wide Cytosine Methylation
Profiling 299
Masako Suzuki and John M Greally
18 1 Introduction 299
18 2 Genome-Wide DNA Methylation Analysis 299
18 3 What is HELP-Tagging? 300
18 3 1 When is HELP-Tagging the Preferred Cytosine Methylation Assay? 301
18 4 Methods and Protocols 301
18 4 1 Reagents, Materials, and Equipment 301
18 4 2 Buffers and Adapters for HELP-Tagging Library Preparation 302
18 4 3 Precautions 303
18 4 4 DNA Samples for HELP-Tagging Library Preparation 303
18 4 5 HELP-Tagging Library Preparation 304
18 4 6 Illumina Sequencing 307
18 5 Applications 308
18 6 Perspectives 308
References 309
19 Second-Generation Sequencing Library Preparation: In Vitro Tagmentation
via Transposome Insertion 311
Fraz Syed
19 1 Introduction 311
19 2 Methods and Protocols 313
19 2 1 Materials 313
19 2 2 Methods 314
19 3 Perspectives 321
References 321
Part Two Next-Generation Tag-Based Sequencing 323
20 Moving Towards Third-Generation Sequencing Technologies 325
Karolina Janitz and MichalJanitz
20 1 Introduction 325
20 2 Differences Between NGS and Sanger Sequencing 326
20 3 Preparation of Templates for Sequencing 326
20 3 1 Next Generation: Single-Molecule Templates 327
20 4 Real-Time Sequencing ,-,327
20 5 Nanopore Sequencing 328
20 6 Ion Torrent Electronic Sequencing 329
20 7 Genome Enrichment 331
20 8 Advantages of NGS 331
20 9 Problem of Short Reads 333
20 10 Perspectives 335
References 335
21 Beyond Tags to Full-Length Transcripts 337
Mohammed Mohiuddin, Stephen Hutchison, and Thomas Jarvie
21 1 Introduction 337
21 2 Generation of Full-Length Transcriptomes 338
21 2 1 mRNA Fragmentation and Preparation for Sequencing 339
21 211 Step 1: mRNA Preparation 339
21 212 Step 2: Fragmentation 339
21 213 Step 3: First-Strand Synthesis 340
21 214 Step 4: Second-Strand Synthesis and Library Preparation 340
21 2 2 Sequencing of Full-Length Transcriptomes 341
21 3 Methods 342
21 3 1 Assembly 342
21 3 2 Mapping 343
21 4 Applications 344
21 4 1 Model Organisms 344
21 4 2 Fusion Transcript Detection 347
21 4 3 Digital Gene Expression 349
21 4 4 Allele-Specific Expression 349
21 5 Perspectives 350
References 351
22 Helicos Single-Molecule Sequencing for Accurate Tag-Based RNA
Quantitation 353
John F Thompson, Tal Raz, and Patrice M Milos
22 1 Introduction 353
22 2 Methods and Protocols 355
22 2 1 Reagents, Required Primers, and Thermocycler Programs 355
22 2 2 Standard Precautions 357
22 2 3 DGE Sample Preparation 357
22 3 Applications 362
22 4 Perspectives 364
References 365
23 Total RNA-seq: Complete Analysis of the Transcriptome Using Illumina
Sequencing-by-Synthesis Sequencing 367
Shujun Luo, Ceoffrey P Smith, Irina Khrebtukova, and Cary P Schroth
23 1 Introduction 367
23 2 Total RNA-Seq 368
23 3 Methods and Protocols 369
23 3 1 Key Materials 369
23 3 2 Method 370
23 4 Total RNA-Seq Data Collection and Interpretation 378
23 5 Applications 380
References 381
Part Three Bioinformatics for Tag-Based Technologies 383
24 Computational Infrastructure and Basic Data Analysis for Next-Generation
Sequencing 385
David Sexton
24 1 Introduction 385
24 2 Background 386
24 3 Getting Started with the Next-Generation Manufacturers 387
24 4 Infrastructure and Data Analysis 388
24 4 1 Computational Considerations 388
24 4 2 Data Dynamics 389
24 4 3 Software and Postanalysis 390
24 4 4 Staffing Requirements 391
24 5 Applications 392
24 6 Perspectives 392
25 CLC Bio Integrated Platform for Handling and Analysis of Tag Sequencing
Data 393
Roald Forsberg, Soren Monsted, and Anne-Mette Hein
25 1 Introduction 393
25 2 Main Components and Features 394
25 2 1 Data Flow and Data Back-End 394
25 2 2 CLC Genomics Workbench 394
25 2 3 CLC Genomics Server 395
25 2 4 APIs 396
25 2 5 Acceleration of Analysis 396
25 3 Applications 396
25 3 1 First-Level Analysis 396
25 311 Import 396
25 312 Demultiplexing 397
25 313 Trim and Quality Control 397
25 3 2 Second and Third Levels - Application-Specific Steps 398
25 321 RNA-Seq 398
25 322 SmallRNA-Seq 399
25 323 Tag-Seq 400
25 324 ChlP-Seq 401
25 3 3 Fourth Level - Expression Analysis 403
25 4 Perspectives 404
References 405
26 Multidimensional Context of Sequence Tags:
Biological Data Integration 407
Korbinian Crote and Thomas Werner
26 1 Introduction 407
26 2 Methods and Strategies 408
26 2 1 Annotation Links Sequence Tags (Reads) to Biology 408
26 2 2 Application of the Methods and Strategies 410
26 2 3 Only Positive Results are Conclusive 411
26 2 4 Automatic Workflow: ChlP-seq of Peroxisome Proliferator-Activated
Receptor-y 412
26 3 Perspectives 414
References 415
27 Experimental Design and Quality Control of Next-Generation Sequencing
Experiments 477
Peter AC 't Hoen, Matthew S Hestand, Judith M Boer, Yuching Lai,
Maarten van Iterson, Michiel van Calen, Henk P Buermans,
andjohan T den Dunnen
27 1 Introduction 417
27 2 Choice of Platform 417
27 2 1 Read Length and Number of Reads 418
27 2 2 Single-End versus Paired-End Sequencing 419
27 2 3 Platform-Specific Advantages and Disadvantages 419
27 3 Sequencing Depth 420
27 3 1 Expression Profiling 420
27 3 2 ChlP-Seq: Relation Enrichment Factor and Sequencing Depth 421
27 3 3 Barcoding 422
27A Replicates, Randomization, and Statistical Testing 422
27A I Technical and Biological Replicates 422
27 4 2 Technical Variability 423
27' 4 3 Biological Replicates Increase Accuracy 424
21' 4 4 Sample Size 424
27 4 5 Importance of Randomizing Samples 424
27 5 Experimental Controls 425
27 5 1 Spike-Ins 425
27 5 2 Negative Controls in ChlP-Seq Experiments 426
27 6 General Quality Assessment 427
27 6 1 Nucleotide Frequency Characteristics 428
27 6 2 Percentage Duplicate Reads 428
27 7 Platform-Specific Quality Scores 428
27 7 1 Sanger, Roche, Illumina, and SOLiD Quality Scores 429
27 7 2 Conversion and Visualization of Quality Scores 429
27 8 Quality Checks After Alignment 430
27 8 1 Percentage of Reads Aligned and Percentage in Repeat Regions 430
27 8 2 DeepSAGE: Percentage 21-22Mers 430
27 8 3 RNA-Seq: Percentage Tags in Annotated Transcripts 430
27 8 4 miRNA Profiling: Percentage in Annotated miRNAs 430
27 8 5 ChlP-Seq: Enrichment 430
27 8 6 Correlation Measures 431
27 9 What Can Go Wrong 431
27 9 1 Sample Swaps 431
27 9 2 Contamination 431
27 10 Perspectives 432
References 432
28 UTGB Toolkit for Personalized Genome Browsers 435
Taro L Saitojun Yoshimura, Budrul Ahsan, Atsushi Sasaki, Reginaldo Kurosh,
and Shinichi Morishita
28 1 Introduction 435
28 2 Overview of the UTGB Toolkit 436
28 2 1 Availability of the UTGB Toolkit 438
28 3 Methods 438
28 3 1 Installation of the UTGB Toolkit 438
28 311 Prerequisites 438
28 312 Easy Installer 438
28 313 Mac OS X and Linux 438
28 314 Windows 439
28 3 2 Running the UTGB Toolkit 439
28 3 3 Viewing Help Messages 439
28 3 4 Creating a new UTGB Project 440
28 3 5 Building a Genome Browser 441
28 3 6 Launching a Portable Web Server 441
28 3 7 Configuring Track Views 441
28 3 8 Adding a New Track 442
28 381 FastaTrack 442
28 382 ReadTrack 442
28 383 WigTrack 443
28 384 Adding Keyword Search 443
28 3 9 Switching Views 443
28 3 10 Publishing Your Genome Browser 443
28 3 11 Manual Installation of UTGB Toolkit (Optional) 444
28 3 11 1 Windows 444
28 3 11 2 Mac OS X and Linux 444
28 3 12 Developing Your Own Tracks 444
28 4 Applications 444
28 4 1 Portable Web Server for Quickly Browsing Local Resources 444
28 4 2 Portable Database Engine 445
28 4 3 Web Application Development Framework 446
28 431 Server-Side Programming Support 446
28 432 Web Action 446
28 433 Database Connection 446
28 434 Object-Database Mapping 446
28 5 Perspectives 447
References 447
29 Beyond the Pipelines: Cloud Computing Facilitates Management,
Distribution, Security, and Analysis of High-Speed Sequencer Data 449
Boris Umylny and Richard S J Weisburd
29 1 Introduction 449
29 2 Data Management 450
29 2 1 Data Quantity 450
29 2 2 HSSs 451
29 2 3 Data Analysis 452
29 2 4 Data Size 453
29 3 Distribution 454
29 3 1 Collaboration 454
29 3 2 Distribution of Data, Annotations, and Analysis Tools 455
29 4 Analysis 456
29 4 1 Integrating Data Repositories and Analytics 456
29 4 2 Integrating HSS Discovery Pipelines with Annotation Data 458
29 4 3 Integrating HSS and Traditional Analysis Algorithms 459
29 4 4 Cloud-Based Infrastructure 461
29 5 Security 462
29 6 Healthcare Data and Privacy Issues 464
29 7 Sample Evaluation of a Vendor Solution 465
29 8 Perspectives 465
References 467
30 Computational Methods for the Identification of MicroRNAs from Small
RNA Sequencing Data 469
Eugene Berezikov
30 1 Introduction 469
30 2 Implementing the miR-Intess Pipeline 470
30 2 1 Preprocessing of Small RNA Sequencing Data 470
30 2 2 Mapping of Small RNA Reads to the Genome 471
30 2 3 Annotation 471
30 2 4 Identification of Hairpin Structures 472
30 2 5 miRNA Signatures 472
30 2 6 miRNA Expression Profiles 474
30 3 Applications 474
References 474
Glossary 477
Link Collection for Next-Generation Sequencing 565
Index 575 |
| any_adam_object | 1 |
| author2 | Harbers, Matthias |
| author2_role | edt |
| author2_variant | m h mh |
| author_facet | Harbers, Matthias |
| building | Verbundindex |
| bvnumber | BV039583878 |
| classification_rvk | WC 4460 |
| ctrlnum | (OCoLC)856788167 (DE-599)DNB1011200392 |
| dewey-full | 572.330285 572.8028 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.330285 572.8028 |
| dewey-search | 572.330285 572.8028 |
| dewey-sort | 3572.330285 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV039583878 |
| illustrated | Illustrated |
| indexdate | 2024-07-21T00:10:06Z |
| institution | BVB |
| isbn | 9783527328192 352732819X |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024435115 |
| oclc_num | 856788167 |
| open_access_boolean | |
| owner | DE-11 DE-19 DE-BY-UBM |
| owner_facet | DE-11 DE-19 DE-BY-UBM |
| physical | XXV, 581 S. Ill., graph. Darst. 240 mm x 170 mm |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Wiley-Blackwell |
| record_format | marc |
| spelling | Tag-based next generation sequencing ed. by Matthias Harbers ... Weinheim Wiley-Blackwell 2012 XXV, 581 S. Ill., graph. Darst. 240 mm x 170 mm txt rdacontent n rdamedia nc rdacarrier Literaturangaben Sequenzanalyse Chemie (DE-588)4132277-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Sequenzanalyse Chemie (DE-588)4132277-0 s DE-604 Harbers, Matthias edt X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3718077&prov=M&dok_var=1&dok_ext=htm Inhaltstext HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024435115&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Tag-based next generation sequencing Sequenzanalyse Chemie (DE-588)4132277-0 gnd |
| subject_GND | (DE-588)4132277-0 (DE-588)4143413-4 |
| title | Tag-based next generation sequencing |
| title_auth | Tag-based next generation sequencing |
| title_exact_search | Tag-based next generation sequencing |
| title_full | Tag-based next generation sequencing ed. by Matthias Harbers ... |
| title_fullStr | Tag-based next generation sequencing ed. by Matthias Harbers ... |
| title_full_unstemmed | Tag-based next generation sequencing ed. by Matthias Harbers ... |
| title_short | Tag-based next generation sequencing |
| title_sort | tag based next generation sequencing |
| topic | Sequenzanalyse Chemie (DE-588)4132277-0 gnd |
| topic_facet | Sequenzanalyse Chemie Aufsatzsammlung |
| url | http://deposit.dnb.de/cgi-bin/dokserv?id=3718077&prov=M&dok_var=1&dok_ext=htm http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024435115&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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