Microtubule dynamics: methods and protocols
Saved in:
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York [u.a.]
Humana Press
2011
|
| Series: | Methods in molecular biology
777 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Literaturangaben |
| Physical Description: | XI, 319 S. Ill., graph. Darst. |
| ISBN: | 9781617792519 |
Staff View
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Record in the Search Index
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| adam_text | Titel: Microtubule dynamics
Autor: Straube, Anne
Jahr: 2011
Contents
Preface............................................................ p
Contributors........................................................ ix
1 How to Measure Microtubule Dynamics?.............................. 1
Anne Straube
2 Purification of Tubulin from Porcine Brain............................. 15
Christof her Gell, Claire T. Friel, Barbara Borgonovo,
David N. Drechsel, Anthony A. Hyman, and Jonathon Howard
3 Purification of Tubulin from the Fission Yeast Schizosaccharomyces pombe....... 29
Douglas R. Drummond, Susan Kain, Anthony Newcombe,
Christina Hoey, Miho Katsuki, and Robert A. Cross
4 Generation of Differentially Polyglutamylated Microtubules................ 57
Benjamin Lacroix and Carsten Janke
5 Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms.......... 71
Laurence Lafanechère and Didier Job
6 aß-Tubulin and Microtubule-Binding Assays........................... 87
Jaime N. Campbell and Kevin C. Slep
7 Analysis of Microtubule Assembly Kinetics Using Turbidimetry............. 99
Felicia Gaskin
8 Isolation of Centrosomes from Cultured Cells.......................... 107
Simone Reber
9 Preparation of Dual-Color Polarity-Marked Fluorescent
Microtubule Seeds............................................... 117
Miho Katsuki, Etsuko Muto, and Robert A. Cross
10 Reconstitution and Quantification of Dynamic Microtubule
End Tracking In Vitro Using TIRF Microscopy......................... 127
Ivo A. Telley, Peter Bieling, and Thomas Surrey
11 Force Generation by Dynamic Microtubules In Vitro..................... 147
Svenja-Marei Kalisch, Liedewij Laan, and Marileen Dogterom
12 Variations on the Single-Molecule Assay
for Microtubule-Associated Proteins and Kinesins........................ 167
Susanne Bechstedt, Michal Wieczorek, Michael Noujaim,
and Gary J. Brouhard
13 Analysing the ATP Turnover Cycle of Microtubule Motors................. 177
Claire T. Friel, Clive R. Bagshaw, and Jonathon Howard
14 Cryo-electron Tomography of Microtubules Assembled
In Vitro from Purified Components.................................. 193
Frédéric M. Coquelle, Sophie Blestel, Claire Heichette,
Isabelle Arnal, Charles Kervrann, and Denis Chrétien
viii Contents
15 Kinetochore Microtubules, Analyzed by Correlated Light
and Immunoelectron Microscopy.................................... 209
Andreas Merdes and Jan De Mey
16 Photoactivatable-GFP-ct-Tubulin as a Tool to Study
Microtubule Plus-End Turnover in Living Human Cells................... 223
Catarina P. Samora and Andrew D. McAinsh
17 Quantification of Asymmetric Microtubule Nucleation
at Subcellular Strucrures........................................... 235
Xiaodong Zhu and Irina Kaverina
18 Investigating Microtubule Dynamic Instability
Using Microtubule-Targeting Agents................................. 245
Stéphane Honore and Diane Braguer
19 Laser Ablation of the Microtubule Cytoskeleton: Setting
Up and Working with an Ablation System.............................. 261
Nicola Maghelli and Iva M. Tolié-N0rrelykke
20 Isolation, Identification, and Validation of Microtubule-Associated
Proteins from Drosophila Embryos................................... 273
Robin Antrobus and James G. Wakefield
21 Isolation of Novel +TIPs and Their Binding Partners Using
Affinity Purification Techniques..................................... 293
Ka Lou Tu, Nanda Keijzer, Casper C. Hoogenraad,
and Anna Akhmanova
Index............................................................. 317
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| institution | BVB |
| isbn | 9781617792519 |
| language | English |
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| physical | XI, 319 S. Ill., graph. Darst. |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Microtubule dynamics methods and protocols ed. by Anne Straube New York [u.a.] Humana Press 2011 XI, 319 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 777 Springer protocols Literaturangaben Mikrotubulus (DE-588)4169864-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mikrotubulus (DE-588)4169864-2 s DE-604 Straube, Anne 1975- (DE-588)124554598 edt Methods in molecular biology 777 (DE-604)BV035362695 777 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024433538&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| subject_GND | (DE-588)4169864-2 (DE-588)4143413-4 |
| title | Microtubule dynamics methods and protocols |
| title_auth | Microtubule dynamics methods and protocols |
| title_exact_search | Microtubule dynamics methods and protocols |
| title_full | Microtubule dynamics methods and protocols ed. by Anne Straube |
| title_fullStr | Microtubule dynamics methods and protocols ed. by Anne Straube |
| title_full_unstemmed | Microtubule dynamics methods and protocols ed. by Anne Straube |
| title_short | Microtubule dynamics |
| title_sort | microtubule dynamics methods and protocols |
| title_sub | methods and protocols |
| topic | Mikrotubulus (DE-588)4169864-2 gnd |
| topic_facet | Mikrotubulus Aufsatzsammlung |
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