Induction of HO-1 on endothelial cells via pI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Gießen
VVB Laufersweiler
2010
|
| Edition: | 1. Aufl. |
| Series: | Edition scientifique
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | VIII, 79 S. graph. Darst., Kt. 21 cm |
| ISBN: | 9783835956353 |
Staff View
MARC
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| 100 | 1 | |a Rahayu, Puji |e Verfasser |0 (DE-588)142937231 |4 aut | |
| 245 | 1 | 0 | |a Induction of HO-1 on endothelial cells via pI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients |c submitted by Puji Rahayu |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Gießen |b VVB Laufersweiler |c 2010 | |
| 300 | |a VIII, 79 S. |b graph. Darst., Kt. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Edition scientifique | |
| 502 | |a Zugl.: Gießen, Univ., Diss., 2010 | ||
| 650 | 7 | |a dengue virus |2 cabt | |
| 650 | 7 | |a arboviruses |2 cabt | |
| 650 | 7 | |a dengue |2 cabt | |
| 650 | 7 | |a human diseases |2 cabt | |
| 650 | 7 | |a viral diseases |2 cabt | |
| 650 | 7 | |a flavivirus |2 cabt | |
| 650 | 7 | |a disease vectors |2 cabt | |
| 650 | 7 | |a culicidae |2 cabt | |
| 650 | 7 | |a immunology |2 cabt | |
| 650 | 7 | |a immune response |2 cabt | |
| 650 | 7 | |a immunological techniques |2 cabt | |
| 650 | 7 | |a antibodies |2 cabt | |
| 650 | 7 | |a elisa |2 cabt | |
| 650 | 7 | |a polymerase chain reaction |2 cabt | |
| 650 | 7 | |a flow cytometry |2 cabt | |
| 650 | 7 | |a immunoprecipitation tests |2 cabt | |
| 650 | 7 | |a western blotting |2 cabt | |
| 650 | 7 | |a in vitro |2 cabt | |
| 650 | 7 | |a endothelium |2 cabt | |
| 650 | 7 | |a cell culture |2 cabt | |
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Record in the Search Index
| _version_ | 1804147992194711552 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
ABBREVIATIONS I
CONTENTS IV
LIST OF TABLES VII
LIST OF FIGURES VIII
CHAPTER 1 INTRODUCTION 1
1.1 BASIC BIOLOGY AND EPIDEMIOLOGY OF DENGUE 1
1.1.1 DENGUE DISEASE 1
1.1.2 CLINICAL AND PATHOLOGICAL FINDINGS ON DENGUE VIRUS INFECTION 2
1.1.3 PATHOGENESIS OF SEVERE DENGUE VIRUS INFECTION 4
1.1.4 VIRUS STRUCTURE 6
1.2 DENGUE DISEASE AND ENDOTHELIAL CELLS 8
1.2.1 CROSS-REACTION OF DENGUE ANTIBODIES WITH ENDOTHELIAL CELLS 8
1.2.2 DENGUE VIRUS-INDUCED ANTI-ENDOTHELIAL CELLS AUTOANTIBODY 10
1.2.3 IMMUNOPATHOGENETIC EFFECTS OF DENGUE VIRUS INFECTION ON
ENDOTHELIAL CELLS 10
1.3 HEME OXYGENASE (HO) 12
1.3.1 ISOFORMS OF HO 12
1.3.2 FUNCTIONAL SIGNIFICANCE OF HO-1 12
1.3.3 PHYSIOLOGICAL FUNCTION OF HO-1 14
1.3.4 SIGNALING PATHWAY OF HO-1 15
1.4 OBJECTIVES OF THE STUDY 16
CHAPTER 2 MATERIALSAND METHODS 17
2.1 MATERIALS 17
2.1.1 CHEMICALS AND REAGENTS 17
2.1.2 PHARMACOLOGICAL COMPOUNDS 18
2.1.3 MARKERS 18
2.1.4 KITS 19
2.1.5 MATERIALS OF CELL CULTURE 19
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1009824716
DIGITALISIERT DURCH
IMAGE 2
2.1.6 CELL CULTURES 20
2.1.7 ANTIGEN AND ANTIBODIES 20
2.1.8 OTHER MATERIALS 21
2.1.9 INSTRUMENTS 21
2.2 METHODS 23
2.2.1 SAMPLE COLLECTION 23
2.2.2 IGG PURIFICATION 23
2.2.3 DETERMINATION OF ANTI-NS1 ANTIBODIES BY SOLID PHASE ELISA... 23
2.2.4 CELL CULTURE PREPARATION 24
2.2.5 CELL CULTURE STIMULATION 24
2.2.6 WESTERN BLOTTING ANALYSIS 25
2.2.7 IMMUNOPRECIPITATION 26
2.2.8 FLOW CYTOMETRY ANALYSIS 27
2.2.8.1 ANALYSIS OF CELL APOPTOSIS 27
2.2.8.2 ANALYSIS OF ROS PRODUCTION 27
2.2.8.3 ANALYSIS OF ANTIBODY BINDING ON ENDOTHELIAL CELLS 28
2.2.9 ANALYSIS OF ENDOTHELIAL PERMEABILITY 28
2.2.10 QUANTIFICATION ANALYSIS 29
2.2.11 STATISTICAL ANALYSIS 29
CHAPTER 3 RESULTS 30
3.1 ANTI-NS1 ANTIBODIES IN DENGUE VIRUS INFECTED PATIENTS 30
3.2 THE INFLUENCE OF ANTI-NS1 ANTIBODIES ON THE REGULATION OF HO-1 32
3.3 INHIBITION OF ANTI-NS1 ANTIBODY-MEDIATED HO-1 INDUCTION BY
NS1 ANTIGEN 36
3.4 ANTI-NS1 ANTIBODIES ACTIVATE HO-1 VIA PL3K PATHWAY 37
3.5 ANTI-NS1 ANTIBODIES INCREASE ACCUMULATION OF CELLULAR ROS 39
3.6 ANTI-NS1 ANTIBODIES INDUCE APOPTOSIS ON ENDOTHELIAL CELLS 41
3.7 ANTI-NS1 ANTIBODIES BINDING ONTO ENDOTHELIAL CELLS 42
3.8 ANTI-NS1 ANTIBODIES OF DHF PATIENTS REACT WITH PDI ANTIGEN
ON ENDOTHELIAL CELLS 43
IMAGE 3
3.9 INHIBITION OF PDI ABOLISHES HO-1 UPREGULATION MEDIATED
BY ANTI-NS1 ANTIBODIES 45
3.10 PERMEABILITY DISTURBANCE OF ENDOTHELIAL CELLS BY ANTI-NS1
ANTIBODIES 47
CHAPTER 4 DISCUSSION 48
4.1 ANTI-NS1 ANTIBODIES CAUSE ACCUMULATION OF CELLULAR ROS,
APOPTOSIS, AND PERMEABILITY DISTURBANCE ON ENDOTHELIAL CELLS 48
4.2 ANTI-NS1 ANTIBODIES REGULATE THE ANTI-APOPTOTIC HO-1
ON ENDOTHELIAL CELLS VIA ACTIVATION OF PL3K 52
CHAPTER 5 SUMMARY 56
CHAPTER 6 ZUSAMMENFASSUNG 58
CHAPTER 7 REFERENCES 60
ACKNOWLEDGEMENTS 79
|
| any_adam_object | 1 |
| author | Rahayu, Puji |
| author_GND | (DE-588)142937231 |
| author_facet | Rahayu, Puji |
| author_role | aut |
| author_sort | Rahayu, Puji |
| author_variant | p r pr |
| building | Verbundindex |
| bvnumber | BV039149293 |
| collection | digit |
| ctrlnum | (OCoLC)690863358 (DE-599)DNB1009824716 |
| dewey-full | 614.58852 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 614 - Forensic medicine; incidence of disease |
| dewey-raw | 614.58852 |
| dewey-search | 614.58852 |
| dewey-sort | 3614.58852 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| edition | 1. Aufl. |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV039149293 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:00:03Z |
| institution | BVB |
| isbn | 9783835956353 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024167135 |
| oclc_num | 690863358 |
| open_access_boolean | |
| owner | DE-188 |
| owner_facet | DE-188 |
| physical | VIII, 79 S. graph. Darst., Kt. 21 cm |
| psigel | digit |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | VVB Laufersweiler |
| record_format | marc |
| series2 | Edition scientifique |
| spelling | Rahayu, Puji Verfasser (DE-588)142937231 aut Induction of HO-1 on endothelial cells via pI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients submitted by Puji Rahayu 1. Aufl. Gießen VVB Laufersweiler 2010 VIII, 79 S. graph. Darst., Kt. 21 cm txt rdacontent n rdamedia nc rdacarrier Edition scientifique Zugl.: Gießen, Univ., Diss., 2010 dengue virus cabt arboviruses cabt dengue cabt human diseases cabt viral diseases cabt flavivirus cabt disease vectors cabt culicidae cabt immunology cabt immune response cabt immunological techniques cabt antibodies cabt elisa cabt polymerase chain reaction cabt flow cytometry cabt immunoprecipitation tests cabt western blotting cabt in vitro cabt endothelium cabt cell culture cabt Protein NS1 Denguevirus (DE-588)7739941-9 gnd rswk-swf Hämorrhagisches Denguefieber (DE-588)7739001-5 gnd rswk-swf Permeabilität (DE-588)4173828-7 gnd rswk-swf Endothel (DE-588)4014675-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Hämorrhagisches Denguefieber (DE-588)7739001-5 s Endothel (DE-588)4014675-3 s Permeabilität (DE-588)4173828-7 s Protein NS1 Denguevirus (DE-588)7739941-9 s DE-604 Erscheint auch als Online-Ausgabe Rahayu, Puji Induction of HO-1 on endothelial cells via PI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024167135&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rahayu, Puji Induction of HO-1 on endothelial cells via pI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients dengue virus cabt arboviruses cabt dengue cabt human diseases cabt viral diseases cabt flavivirus cabt disease vectors cabt culicidae cabt immunology cabt immune response cabt immunological techniques cabt antibodies cabt elisa cabt polymerase chain reaction cabt flow cytometry cabt immunoprecipitation tests cabt western blotting cabt in vitro cabt endothelium cabt cell culture cabt Protein NS1 Denguevirus (DE-588)7739941-9 gnd Hämorrhagisches Denguefieber (DE-588)7739001-5 gnd Permeabilität (DE-588)4173828-7 gnd Endothel (DE-588)4014675-3 gnd |
| subject_GND | (DE-588)7739941-9 (DE-588)7739001-5 (DE-588)4173828-7 (DE-588)4014675-3 (DE-588)4113937-9 |
| title | Induction of HO-1 on endothelial cells via pI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients |
| title_auth | Induction of HO-1 on endothelial cells via pI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients |
| title_exact_search | Induction of HO-1 on endothelial cells via pI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients |
| title_full | Induction of HO-1 on endothelial cells via pI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients submitted by Puji Rahayu |
| title_fullStr | Induction of HO-1 on endothelial cells via pI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients submitted by Puji Rahayu |
| title_full_unstemmed | Induction of HO-1 on endothelial cells via pI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients submitted by Puji Rahayu |
| title_short | Induction of HO-1 on endothelial cells via pI3K signaling pathway by anti-NS1 antibodies in dengue virus infected patients |
| title_sort | induction of ho 1 on endothelial cells via pi3k signaling pathway by anti ns1 antibodies in dengue virus infected patients |
| topic | dengue virus cabt arboviruses cabt dengue cabt human diseases cabt viral diseases cabt flavivirus cabt disease vectors cabt culicidae cabt immunology cabt immune response cabt immunological techniques cabt antibodies cabt elisa cabt polymerase chain reaction cabt flow cytometry cabt immunoprecipitation tests cabt western blotting cabt in vitro cabt endothelium cabt cell culture cabt Protein NS1 Denguevirus (DE-588)7739941-9 gnd Hämorrhagisches Denguefieber (DE-588)7739001-5 gnd Permeabilität (DE-588)4173828-7 gnd Endothel (DE-588)4014675-3 gnd |
| topic_facet | dengue virus arboviruses dengue human diseases viral diseases flavivirus disease vectors culicidae immunology immune response immunological techniques antibodies elisa polymerase chain reaction flow cytometry immunoprecipitation tests western blotting in vitro endothelium cell culture Protein NS1 Denguevirus Hämorrhagisches Denguefieber Permeabilität Endothel Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024167135&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT rahayupuji inductionofho1onendothelialcellsviapi3ksignalingpathwaybyantins1antibodiesindenguevirusinfectedpatients |