Follicular penetration of nano-sized particulate FA-PLGA and the dye RhBITC in dorsal dog and rat skin, and porcine ear skin as an in vitro model for the human integument:
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| Format: | Thesis Book |
| Language: | English |
| Published: |
Berlin
Mensch und Buch Verl.
2010 [erschienen] 2011
|
| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis |
| Item Description: | Nebentitel: Follikuläre Penetration von nanogroßen FA-PLGA-Partikeln und dem Farbstoff RhBITC in die Rückenhaut von Hunden und Ratten, sowie in die Ohrenhaut von Schweinen als in-vitro-Modell für die menschliche Haut |
| Physical Description: | V, 108 S. Ill., graph. Darst. |
| ISBN: | 9783866649613 |
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| 100 | 1 | |a Knorr, Fanny Esther |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Follicular penetration of nano-sized particulate FA-PLGA and the dye RhBITC in dorsal dog and rat skin, and porcine ear skin as an in vitro model for the human integument |c vorgelegt von Fanny Esther Knorr |
| 246 | 1 | 3 | |a Follikuläre Penetration von nanogroßen FA-PLGA-Partikeln und dem Farbstoff RhBITC in die Rückenhaut von Hunden und Ratten, sowie in die Ohrenhaut von Schweinen als in-vitro-Modell für die menschliche Haut |
| 264 | 1 | |a Berlin |b Mensch und Buch Verl. |c 2010 [erschienen] 2011 | |
| 300 | |a V, 108 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Nebentitel: Follikuläre Penetration von nanogroßen FA-PLGA-Partikeln und dem Farbstoff RhBITC in die Rückenhaut von Hunden und Ratten, sowie in die Ohrenhaut von Schweinen als in-vitro-Modell für die menschliche Haut | ||
| 502 | |a Berlin, Freie Univ., Diss., 2010 | ||
| 650 | 7 | |a Hair follicles |2 cabt | |
| 650 | 7 | |a skin |2 cabt | |
| 650 | 7 | |a penetration |2 cabt | |
| 650 | 7 | |a particles |2 cabt | |
| 650 | 7 | |a size |2 cabt | |
| 650 | 7 | |a extraction |2 cabt | |
| 650 | 7 | |a pigs |2 cabt | |
| 650 | 7 | |a dogs |2 cabt | |
| 650 | 7 | |a rats |2 cabt | |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:kobv:188-fudissthesis000000022855-1 |
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| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-022580682 | ||
Record in the Search Index
| _version_ | 1804145734035963904 |
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| adam_text | IMAGE 1
TABLE OF CONTENTS
TABLE OF CONTENTS
ABBREVIATIONS AND ACRONYMS IV
1. INTRODUCTION AND RESEARCH AIMS 1
1.1. INTRODUCTION 2
1.2. RESEARCH AIMS 3
2. LITERATURE REVIEW 5
2.1. SKIN 6
2.1.1. EPIDERMIS 6
2.1.2. DENNIS 8
2.1.3. HYPODERMIS 10
2.1.4. APPENDAGES 10
HAIRFOLLICLES 10
SEBACEOUS GLANDS 14
SWEAT GLANDS 15
ARRECTOR PILI MUSCLE 16
2.2. PENETRATION PATHWAYS INTO THE SKIN 16
2.2.1. INTERCELLULAR AND TRANSCELLULAR PENETRATION 17
2.2.2. FOLLICULAR PENETRATION 17
2.2.3. METHODS FOR ANALYZING INTERCELLULAR AND FOLLICULAR PENETRATION 19
TAPE STRIPPING 19
CYANOACRYLATE FOLLICULAR BIOPSY 20
DIFFERENTIAL STRIPPING 20
2.3. FOLLICULAR PENETRATION OF NANO-SIZED PARTICIPATES 21
2.3.1. NANO-SIZEDPANICULATE PLGA 22
2.3.2. FLUORESCENT MARKERS 23
2.3.3. METHODS FOR DETECTING FLUORESCENT MARKERS IN THE SKIN 24
FLUORESCENCE MICROSCOPY 24
CONFOCAL LASER SCANNING MICROSCOPY 25
FLUORESCENCE SPECTROSCOPY 26
3. MATERIALS AND METHODS 28
3.1. EXPERIMENTS ON THE FOLLICULAR PENETRATION OF NANO-SIZED PARTICIPATE
FA-PLGA IN DOG, RAT AND PORCINE SKIN 29
3.1.1. MATERIAL 29
FORMULATIONS CONTAINING NANO-SIZEDPARTICULATE FA-PLGA 29
SKIN SAMPLES 29
I
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1011794195
DIGITALISIERT DURCH
IMAGE 2
TABLE OF CONTENTS
3.1.2 METHODS 30
PREPARATION OF THE SKIN SAMPLES 30
APPLICATION OF FORMULATIONS CONTAINING NANO-SIZED PARTICULATE FA-PLGA 30
SKIN BIOPSIES 31
CRYOHISTOLOGICAL CROSS-SECTIONS 31
CONFOCAL LASER SCANNING MICROSCOPY 31
3.2. EXPERIMENTS ON THE SELECTIVE QUANTIFICATION OF FOLLICULAR RHBITC IN
PORCINE SKIN 33
3.2.1. MATERIAL 33
FORMULATION CONTAINING RHBITC 33
SKIN SAMPLES 33
3.2.2. METHODS 34
PREPARATION OF THE SKIN SAMPLES 34
APPLICATION OF THE FORMULATION CONTAINING RHBITC 34
TAPE STRIPPING 34
FLUORESCENCE MICROSCOPY 36
EXTRACTION OF RHBITC 36
FLUORESCENCE SPECTROSCOPY 37
4. RESULTS 38
4.1. THE FOLLICULAR PENETRATION OF NANO-SIZED PARTICULATE FA-PLGA IN
DOG, RAT AND PORCINE SKIN 39
4.1.1. FOLLICULAR PENETRATION OFFA-PLGA 256 AND FA-PLGA430 IN DOG SKIN
39
4.1.2. FOLLICULAR PENETRATION OFFA-PLGA 256 AND FA-PLGA430 IN RAT SKIN
42 4.1.3. FOLLICULAR PENETRATION OF FA-PLGA256 AND FA-PLGA430 IN PORCINE
SKIN 44 4.1.4. COMPARISONS BETWEEN THE FOLLICULAR PENETRATION OF
FA-PLGA256 AND FA-PLGA430 IN DOG, RAT AND PORCINE SKIN 47
4.2. THE SELECTIVE QUANTIFICATION OF FOLLICULAR RHBITC IN PORCINE SKIN
48 4.2.1. FLUORESCENCE MICROSCOPY 48
4.2.2. FLUORESCENCE SPECTROSCOPY 51
5. DISCUSSION 54
5.1. CONSIDERATIONS ON THE FOLLICULAR PENETRATION OF NANO-SIZED
PARTICULATE FA-PLGA IN DOG, RAT AND PORCINE SKIN 55
5.1.1. FOLLICULAR MORPHOMETRIES AND SKIN CONSTITUTIONS 55
HAIR COAT 56
ACTIVE AND INACTIVE HAIR FOLLICLES 56
FOLLICULAR ORIFICES AND INFUNDIBULA 57
SEBACEOUS GLANDS 58
II
IMAGE 3
TABLE OF CONTENTS
BULGE REGION 59
TIGHT JUNCTIONS 60
CAPILLARY NETWORK 60
5.1.2. CHARACTERISTICS OF THE PARTICULATES 61
SIZE 61
AGGREGATION 61
DEGRADATION 61
LEAKAGE OFFLUORESCEINAMINE 62
RISKS AND TOXICITY 62
5.1.3. FUTURE OUTLOOK ON THE FOLLICULAR PENETRATION OF NANO-SIZED
PARTICULATES
IN ANIMALS RELEVANT FOR VETERINARY PRACTITIONERS 63
5.2. CONSIDERATIONS ON THE SELECTIVE QUANTIFICATION OF FOLLICULAR RHBITC
IN
PORCINE SKIN 64
5.2.1. PORCINE SKIN AS AN IN VITRO MODEL FOR THE HUMAN INTEGUMENT 64
5.2.2. METHODOLOGY 65
5.2.3. FUTURE OUTLOOK ON THE SELECTIVE QUANTIFICATION OF DRUGS LOCATED
IN THE FOLLICULAR CANALS OF PORCINE SKIN 65
6. 6.1. 6.1.1.
6.1.2.
6.1.3.
6.2.
7.
8.
9.
10.
1 1,
APPENDIX APPENDIX A: DESCRIPTIVE STATISTICS DOG SKIN RAT SKIN
PORCINE SKIN APPENDIX B: FLUORESCENCE EMISSION OF FOLLICULAR RHBITC
SUMMARY
ZUSAMMENFASSUNG
REFERENCES
ACKNOWLEDGEMENTS
OIRRIRNLIIM VIRAE
67
68 68
69 70 71
75
78
81
107
109
HI
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| any_adam_object | 1 |
| author | Knorr, Fanny Esther |
| author_facet | Knorr, Fanny Esther |
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| author_sort | Knorr, Fanny Esther |
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| bvnumber | BV037428568 |
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| dewey-full | 630 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 630 - Agriculture and related technologies |
| dewey-raw | 630 |
| dewey-search | 630 |
| dewey-sort | 3630 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| format | Thesis Book |
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| indexdate | 2024-07-09T23:24:09Z |
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| isbn | 9783866649613 |
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| spelling | Knorr, Fanny Esther Verfasser aut Follicular penetration of nano-sized particulate FA-PLGA and the dye RhBITC in dorsal dog and rat skin, and porcine ear skin as an in vitro model for the human integument vorgelegt von Fanny Esther Knorr Follikuläre Penetration von nanogroßen FA-PLGA-Partikeln und dem Farbstoff RhBITC in die Rückenhaut von Hunden und Ratten, sowie in die Ohrenhaut von Schweinen als in-vitro-Modell für die menschliche Haut Berlin Mensch und Buch Verl. 2010 [erschienen] 2011 V, 108 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Nebentitel: Follikuläre Penetration von nanogroßen FA-PLGA-Partikeln und dem Farbstoff RhBITC in die Rückenhaut von Hunden und Ratten, sowie in die Ohrenhaut von Schweinen als in-vitro-Modell für die menschliche Haut Berlin, Freie Univ., Diss., 2010 Hair follicles cabt skin cabt penetration cabt particles cabt size cabt extraction cabt pigs cabt dogs cabt rats cabt (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:kobv:188-fudissthesis000000022855-1 http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000022855 kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022580682&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Knorr, Fanny Esther Follicular penetration of nano-sized particulate FA-PLGA and the dye RhBITC in dorsal dog and rat skin, and porcine ear skin as an in vitro model for the human integument Hair follicles cabt skin cabt penetration cabt particles cabt size cabt extraction cabt pigs cabt dogs cabt rats cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Follicular penetration of nano-sized particulate FA-PLGA and the dye RhBITC in dorsal dog and rat skin, and porcine ear skin as an in vitro model for the human integument |
| title_alt | Follikuläre Penetration von nanogroßen FA-PLGA-Partikeln und dem Farbstoff RhBITC in die Rückenhaut von Hunden und Ratten, sowie in die Ohrenhaut von Schweinen als in-vitro-Modell für die menschliche Haut |
| title_auth | Follicular penetration of nano-sized particulate FA-PLGA and the dye RhBITC in dorsal dog and rat skin, and porcine ear skin as an in vitro model for the human integument |
| title_exact_search | Follicular penetration of nano-sized particulate FA-PLGA and the dye RhBITC in dorsal dog and rat skin, and porcine ear skin as an in vitro model for the human integument |
| title_full | Follicular penetration of nano-sized particulate FA-PLGA and the dye RhBITC in dorsal dog and rat skin, and porcine ear skin as an in vitro model for the human integument vorgelegt von Fanny Esther Knorr |
| title_fullStr | Follicular penetration of nano-sized particulate FA-PLGA and the dye RhBITC in dorsal dog and rat skin, and porcine ear skin as an in vitro model for the human integument vorgelegt von Fanny Esther Knorr |
| title_full_unstemmed | Follicular penetration of nano-sized particulate FA-PLGA and the dye RhBITC in dorsal dog and rat skin, and porcine ear skin as an in vitro model for the human integument vorgelegt von Fanny Esther Knorr |
| title_short | Follicular penetration of nano-sized particulate FA-PLGA and the dye RhBITC in dorsal dog and rat skin, and porcine ear skin as an in vitro model for the human integument |
| title_sort | follicular penetration of nano sized particulate fa plga and the dye rhbitc in dorsal dog and rat skin and porcine ear skin as an in vitro model for the human integument |
| topic | Hair follicles cabt skin cabt penetration cabt particles cabt size cabt extraction cabt pigs cabt dogs cabt rats cabt |
| topic_facet | Hair follicles skin penetration particles size extraction pigs dogs rats Hochschulschrift |
| url | http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000022855 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022580682&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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