Human stem cell technology and biology: a research guide and laboratory manual
"Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An...
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Hoboken, NJ
Wiley-Blackwell
2011
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Summary: | "Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers. Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource. A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology. Perspectives written by the world leaders in the field. Introductory chapters will provide background information. The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field"--Provided by publisher |
| Physical Description: | XVII, 419 S. Ill. 1 DVD-ROM, (12 cm) |
| ISBN: | 9780470595459 |
Staff View
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Record in the Search Index
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| adam_text | Titel: Human stem cell technology and biology
Autor: Stein, Gary S.
Jahr: 2011
Foreword ix
Preface xi
Acknowledgments xiii
Editors and Contributors xv
INTRODUCTION
1 Introduction to Pluripotent Stem Cells: Biology and Applications 3
Maria Borowski and Gary S. Stein
2 Researching and Obtaining Established Stem Cell Lines 9
MaiX. Luong, Kelly P. Smith, and Gary S. Stein
LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE:
PLURIPOTENT STEM CELL CULTURE
3 Basics of Cell Culture 19
Alicia Allaire, MaiX. Luong, and Kelly P. Smith
4 The Stem Cell Laboratory 33
Alicia Allaire, Mai X. Luong, and Kelly P. Smith
5 Reagent Preparation 41
Alicia Allaire, Mai X. Luong, and Kelly P. Smith
6 Preparation of Mouse Embryonic Fibroblasts
for Culture of Human Embryonic Stem Cells 59
Meng-fiao Shi, Maria Borowski, and Kimberly Stencel
7 Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts 87
Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel
8 Thawing, Seeding, and Changing the Medium
of Human Embryonic Stem Cells 95
Meng-fiao Shi, Kimberly Stencel, and Maria Borowski
9 Passaging of Human Embryonic Stem Cells
on Inactivated Mouse Embryonic Fibroblast Plates 105
Meng-fiao Shi, Kimberly Stencel, and Maria Borowski
10 Harvesting Human Embryonic Stem Cells for Cryopreservation 119
Meng-fiao Shi, Kimberly Stencel, and Maria Borowski
11 Human Embryonic Stem Cell Culture
on BD Matrigel™ with mTeSR®1 Medium 129
Meng-fiao Shi, Kimberly Stencel, and Maria Borowski
LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE:
CHARACTERIZATION OF PLURIPOTENT STEM CELLS
12 Defining Pluripotency 141
Kelly P. Smith and Mai X. Luong
13 Characterization of Human Embryonic Stem Cells
by Immunofluorescence Microscopy 149
Shirwin M. Pockwinse andPrachi N. Ghule
14 Preparation of Human Embryonic Stem Cell Samples
for Flow Cytometry 159
Meng-fiao Shi, Kimberly Stencel, and Maria Borowski
15 Characterization of Human Embryonic Stem
Cell Gene Expression Using Reverse Transcription
Polymerase Chain Reaction 167
Alicia Allaire, Kelly P. Smith, and MaiX. Luong
16 Determining Pluripotency of Human Embryonic
Stem Cells: Embryoid Body Formation 191
Shirwin M. Pockwinse and Prachi N. Ghule
17 Characterization of Human Embryonic Stem Cells
by Cytogenetics: Karyotyping and Fluorescence
In Situ Hybridization 199
Shirwin M. Pockwinse, Prachi N. Ghule and Anne Higgins
18 High-Resolution Chromatin Immunoprecipitation Assay 211
Beatriz Pérez-Cadahia, Boj an Drobic, and James R. Davie
19 Assaying Pluripotency Via Teratoma Formation 217
M. William Lensch and Tan A. Ince
PERSPECTIVES IN HUMAN STEM CELL TECHNOLOGIES
20 Genomic Analysis of Pluripotent Stem Cells 237
David Lapointe
21 Proteomic Analysis of Human Pluripotent Cells 245
Andy T. Y Lau, Yan-Ming Xu, andfen-Fu Chiu
22 Bioinformatics Strategies for Understanding
Gene Expression in Human Pluripotent Cells 257
Gustavo Glusman, Bruz Marzolf, Kai Wang, fi-Hoon Cho,
Burak Kutlu, and Qiang Tian
23 Epigenetic Analysis of Pluripotent Cells 273
Mojgan Rastegar, Geneviève P. Delcuve, and fames R. Davie
24 Differentiation of Pluripotent Stem Cells: An Overview 289
Jeremy Micah Crook
25 Cellular Reprogramming: Current Technology, Perspectives, and
Generation of Induced Pluripotent Cells 297
Tanja Dominko
SECTäON V
APPLICATIONS OF HUMAN EMBRYONIC STEM CELLS
26 Human Pluripotent Cells: The Biology of Pluripotency 313
Li-Fang Chu and Thomas P. Zwaka
27 Human Pluripotent Cells for Regenerative Medicine:
Potential Applications for Regenerative Medicine 327
Christopher G Ford and Darrell N. Kotton
28 Therapeutic Applications of Human Embryonic Stem Cells 339
Shi-Jiang Lu, Irina Klimanskaya, Edmund Mickunas, and Robert Lanza
29 Human Stem Cells for Drug Screening, Toxicity, Specificity, and
Off-Target Effects 353
Arnaud Lacoste and Mark Burcin
30 Interfacing Stem Cells with Gene Therapy 367
Christian Mueller and Terence R. Flotte
31 Tissue Engineering for Stem Cell Mediated
Regenerative Medicine 377
Janet Zoldan, Thomas P. KraehenbuehL Abigail K. R. Lytton-Jean,
Robert S. Langer, and Daniel G. Anderson
Glossary 401
Index 413
|
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| discipline | Biologie Medizin |
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| spelling | Human stem cell technology and biology a research guide and laboratory manual ed. by Gary S. Stein ... Hoboken, NJ Wiley-Blackwell 2011 XVII, 419 S. Ill. 1 DVD-ROM, (12 cm) txt rdacontent n rdamedia nc rdacarrier "Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers. Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource. A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology. Perspectives written by the world leaders in the field. Introductory chapters will provide background information. The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field"--Provided by publisher Embryonic stem cells / Laboratory manuals Embryonic stem cells / Research SCIENCE / Life Sciences / Cytology bisacsh Stem Cells / Laboratory Manuals Cell Culture Techniques / methods / Laboratory Manuals Stem Cell Research / Laboratory Manuals Technology, Medical / methods / Laboratory Manuals Labortechnik (DE-588)4123602-6 gnd rswk-swf Stammzelle (DE-588)4665329-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Stammzelle (DE-588)4665329-6 s Labortechnik (DE-588)4123602-6 s DE-604 Stein, Gary S. 1943- Sonstige (DE-588)123682584 oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022567605&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Human stem cell technology and biology a research guide and laboratory manual Embryonic stem cells / Laboratory manuals Embryonic stem cells / Research SCIENCE / Life Sciences / Cytology bisacsh Stem Cells / Laboratory Manuals Cell Culture Techniques / methods / Laboratory Manuals Stem Cell Research / Laboratory Manuals Technology, Medical / methods / Laboratory Manuals Labortechnik (DE-588)4123602-6 gnd Stammzelle (DE-588)4665329-6 gnd |
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| title | Human stem cell technology and biology a research guide and laboratory manual |
| title_auth | Human stem cell technology and biology a research guide and laboratory manual |
| title_exact_search | Human stem cell technology and biology a research guide and laboratory manual |
| title_full | Human stem cell technology and biology a research guide and laboratory manual ed. by Gary S. Stein ... |
| title_fullStr | Human stem cell technology and biology a research guide and laboratory manual ed. by Gary S. Stein ... |
| title_full_unstemmed | Human stem cell technology and biology a research guide and laboratory manual ed. by Gary S. Stein ... |
| title_short | Human stem cell technology and biology |
| title_sort | human stem cell technology and biology a research guide and laboratory manual |
| title_sub | a research guide and laboratory manual |
| topic | Embryonic stem cells / Laboratory manuals Embryonic stem cells / Research SCIENCE / Life Sciences / Cytology bisacsh Stem Cells / Laboratory Manuals Cell Culture Techniques / methods / Laboratory Manuals Stem Cell Research / Laboratory Manuals Technology, Medical / methods / Laboratory Manuals Labortechnik (DE-588)4123602-6 gnd Stammzelle (DE-588)4665329-6 gnd |
| topic_facet | Embryonic stem cells / Laboratory manuals Embryonic stem cells / Research SCIENCE / Life Sciences / Cytology Stem Cells / Laboratory Manuals Cell Culture Techniques / methods / Laboratory Manuals Stem Cell Research / Laboratory Manuals Technology, Medical / methods / Laboratory Manuals Labortechnik Stammzelle Aufsatzsammlung |
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