MicroRNAs in development: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
New York [u.a.]
Humana Press
2011
|
| Series: | Methods in molecular biology
732 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | X, 227 S. Ill., graph. Darst. |
| ISBN: | 9781617790829 |
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Record in the Search Index
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| adam_text | Titel: MicroRNAs in development
Autor: Dalmay, Tamas
Jahr: 2011
Contents
Preface................................................................. v
Contributors............................................................. ix
1 In Situ Detection of microRNAs in Animals............................. 1
Dylan Sweetman
2 Detection of microRNAs in Plants by In Situ Hybridisation................. 9
Eva Várallyay and Zoltán Havelda
3 Detecting sRNAs by Northern Blotting................................ 25
Sara López-Gomollón
4 Profiling MicroRNAs by Real-Time PCR............................... 39
Nana Jacobsen, Ditte Andreasen, and Peter Mouritzen
5 Detection of Small RNAs and MicroRNAs Using Deep Sequencing
Technology..................................................... 55
Ericka R. Havecker
6 Detection of MicroRNAs in Prostate Cancer Cells by MicroRNA Array........ 69
Xiaoqing Tang, Xiaohu Tang, JozsefGal, Natasha Kyprianou,
HainingZhu, and Guiliang Tang
7 MicroRNA Knock Down by Cholesterol-Conjugated Antisense
Oligos in Mouse Organ Culture...................................... 89
Sharon Kredo-Russo and Eran Hornstein
8 Protocols for Use of Homologous Recombination Gene Targeting
to Produce MicroRNA Mutants in Drosophila............................ 99
Ta-Wen Chen, Ruifen Weng, and Stephen M. Cohen
9 Engineering Elements for Gene Silencing: The Artificial
MicroRNAs Technology............................................ 121
Pablo Andrés Manavella and Ignacio Rubio-Somoza
10 Mimicry Technology: Suppressing Small RNA Activity in Plants.............. 131
Ignacio Rubio-Somoza and Pablo Andrés Manavella
11 Experimental Validation of MicroRNA Targets Using a Luciferase
Reporter System.................................................. 139
Francisco E. Nicolas
12 Experimental Identification of MicroRNA Targets by Immunoprecipitation
of Argonaute Protein Complexes..................................... 153
Michaela Beitzinger and Gunter Meister
13 Comprehensive Identification of miRNA Target Sites in Live Animals.......... 169
Dimitrios G. Zisoulis, Gene W. Yeo, and Amy E. Pasquinelli
viii Contents
14 Target Validation of Plant microRNAs................................. 187
César Llave, José Manuel Franco-Zorrilla, Roberto Solano,
and Daniel Barajas
15 A High-Throughput Sequencing-Based Methodology to Identify
All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes........... 209
Matthew W. Endres, Rebecca T. Cook, and Brian D. Gregory
Index.............................................................. 225
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| id | DE-604.BV037392690 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T23:23:20Z |
| institution | BVB |
| isbn | 9781617790829 |
| language | English |
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| physical | X, 227 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | MicroRNAs in development methods and protocols ed. by Tamas Dalmay New York [u.a.] Humana Press 2011 X, 227 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 732 Springer protocols Includes bibliographical references and index Small interfering RNA. MicroRNAs Dalmay, Tamas Sonstige oth Erscheint auch als Online-Ausgabe 978-1-61779-083-6 Methods in molecular biology 732 (DE-604)BV035362695 732 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022545541&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | MicroRNAs in development methods and protocols Methods in molecular biology Small interfering RNA. MicroRNAs |
| title | MicroRNAs in development methods and protocols |
| title_auth | MicroRNAs in development methods and protocols |
| title_exact_search | MicroRNAs in development methods and protocols |
| title_full | MicroRNAs in development methods and protocols ed. by Tamas Dalmay |
| title_fullStr | MicroRNAs in development methods and protocols ed. by Tamas Dalmay |
| title_full_unstemmed | MicroRNAs in development methods and protocols ed. by Tamas Dalmay |
| title_short | MicroRNAs in development |
| title_sort | micrornas in development methods and protocols |
| title_sub | methods and protocols |
| topic | Small interfering RNA. MicroRNAs |
| topic_facet | Small interfering RNA. MicroRNAs |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022545541&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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