Intracellular glycation of nuclear DNA, mitochondrial DNA, and cytosolic proteins triggered by endogenous oxidative stress: = Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2011
|
| Schlagworte: | |
| Online-Zugang: | kostenfrei https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-24465 http://d-nb.info/1011059290/34 Inhaltsverzeichnis |
| Beschreibung: | V, 160 S. Ill., graph. Darst. 21 cm |
Internformat
MARC
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| 001 | BV037376840 | ||
| 003 | DE-604 | ||
| 005 | 20160607 | ||
| 007 | t | ||
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| 015 | |a 11,H05 |2 dnb | ||
| 016 | 7 | |a 1011016532 |2 DE-101 | |
| 035 | |a (OCoLC)725235920 | ||
| 035 | |a (DE-599)DNB1011016532 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
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| 084 | |a WD 5360 |0 (DE-625)148202: |2 rvk | ||
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Breyer, Viola |e Verfasser |0 (DE-588)143994174 |4 aut | |
| 245 | 1 | 0 | |a Intracellular glycation of nuclear DNA, mitochondrial DNA, and cytosolic proteins triggered by endogenous oxidative stress |b = Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress |c vorgelegt von Viola Breyer |
| 246 | 1 | 1 | |a Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress |
| 264 | 1 | |c 2011 | |
| 300 | |a V, 160 S. |b Ill., graph. Darst. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Erlangen-Nürnberg, Univ., Diss., 2011 | ||
| 650 | 0 | 7 | |a Oxidativer Stress |0 (DE-588)4353497-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a DNS |0 (DE-588)4070512-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Zellkern |0 (DE-588)4067544-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Glykation |0 (DE-588)4767145-2 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Oxidativer Stress |0 (DE-588)4353497-1 |D s |
| 689 | 0 | 1 | |a Zellkern |0 (DE-588)4067544-0 |D s |
| 689 | 0 | 2 | |a DNS |0 (DE-588)4070512-2 |D s |
| 689 | 0 | 3 | |a Glykation |0 (DE-588)4767145-2 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:29-opus-24465 |
| 856 | 4 | |u https://open.fau.de/handle/openfau/1651 |x Verlag |z kostenfrei |3 Volltext | |
| 856 | 4 | |u https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-24465 |x Resolvingsystem | |
| 856 | 4 | |u http://d-nb.info/1011059290/34 |x Langzeitarchivierung Nationalbibliothek | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022530055&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-022530055 | ||
Datensatz im Suchindex
| _version_ | 1804145660587409408 |
|---|---|
| adam_text | CONTENTS
ABSTRACT
1
ZUSAMMENFASSUNG
7
1
INTRODUCTION
13
1.1
THE
FREE
RADICAL
THEORY
OF
AGING
..................................................................
14
1.2
THE
MITOCHONDRIA
.........................................................................................
14
1.2.1
CITRIC
ACID
CYCLE
AND
FATTY
ACID-OXIDATION
.....................................
16
1.2.2
OXIDATIVE
PHOSPHORYLATION
..............................................................
16
1.2.3
THE
MITOCHONDRIAL
ROLE
IN
AGING
.......................................................
18
1.3
REACTIVE
OXYGEN
SPECIES
..............................................................................
19
1.4 ADVANCED
GLYCATION
END-PRODUCTS
..............................................................
21
1.4.1
THE
MAILLARD
REACTION
......................................................................
21
1.4.2
PHYSIOLOGICAL
CONSEQUENCES
............................................................
21
1.4.3
THE
GLYCATION
THEORY
OF
AGING
.........................................................
28
1.5
MOUSE
MODELS
WITH
REDUCED
SOD
ACTIVITY
..................................................29
1.5.1
MUTANT
MICE
LACKING
COPPER/ZINC
SUPEROXIDE
DISMUTASE
..........
30
1.5.2
MUTANT
MICE
LACKING
MANGANESE
SUPEROXIDE
DISMUTASE
.............
31
1.6
AIMS
OF
THIS
THESIS
........................................................................................
33
2
IN
VITRO
ANALYSIS
OF
DNA
GLYCATION
PRODUCTS
35
2.1
INTRODUCTION
AND
EXPERIMENTAL
DESIGN
......................................................
35
2.1.1
SELECTION
OF
A
REPORTER
GENE
ASSAY
..................................................
36
2.1.2
SELECTION
OF
THE
TRANSFECTION
METHOD
................................................
37
2.1.3
INTRODUCTION
AND
QUANTIFICATION
OF
CEDG
MODIFICATIONS
...........
38
2.1.4
INFLUENCE
OF
CEDG
ADDUCTS
ON
LUCIFERASE
ACTIVITY
IN
HEK
293
T
CELLSINVITRO
.......................................................................................
40
2.2
RESULTS
.............................................................................................................
42
2.2.1
CYTOTOXIC
EFFECT
ON
TRANSFECTED
HEK
293
T
CELLS
..............................
42
2.2.2
QUANTIFICATION
OF
PLASMID
TRANSCRIPTION
...........................................
42
2.2.3
INFLUENCE
OF
CEDG
ADDUCTS
ON
THE
FUNCTIONALITY
OF
THE
AMPICILLIN
RESISTANCE
GENE
......................................................................
44
2.2.4
RESTRICTION
ENZYME
DIGESTION
OF
CEDG-MODIFIED
PLASMID
DNA
.
46
2.3
DISCUSSION
....................................................................................................
47
3
SIMULTANEOUS
ANALYSIS
OF
ENDOGENOUSLY
FORMED
AGES
51
3.1
INTRODUCTION
AND
EXPERIMENTAL
DESIGN
........................................................
51
3.1.1
IN
VITRO
AGING
MODEL
...........................................................................
52
3.2
RESULTS
............................................................................................................
55
3.2.1
DEVELOPMENT
OF
A
METHOD
FOR
THE
SIMULTANEOUS
ISOLATION
OF
NUCLEAR
DNA,
MTDNA,
AND
CYTOSOLIC
PROTEINS
...................................
55
3.2.2
ISOLATION
OF
NUCLEAR
DNA,
MITOCHONDRIAL
DNA,
AND
CYTOSOLIC
PROTEINS
FROM
NIH
3T3
FIBROBLASTS
...................................................
59
3.2.3
CHARACTERIZATION
OF
THE
MITOCHONDRIAL
FRACTION
................................
59
3.2.4
DETERMINATION
OF
OPTIMAL
CONDITIONS
FOR
THE
SENESCENCE-LIKE
GROWTH
ARREST
OF
NIH
3T3
FIBROBLASTS
................................................
61
3.2.5
DETERMINATION
OF
CEDG
CONTENT
OF
NUCLEAR
DNA
AND
MTDNA
...
64
3.2.6
ANALYSIS
OF
PROTEIN
EXPRESSION
AND
AGE
MODIFICATIONS
IN
SENES
CENT
FIBROBLASTS
..................................................................................
64
3.2.7
ANALYSIS
OF
OXIDATIVE
PROTEIN
MODIFICATION
......................................
69
3.2.8
IDENTIFICATION
OF
OXIDIZED
PROTEINS
...................................................
71
3.2.9
IMMUNOPRECIPITATION
OF
ACTIN
AND
SUBSEQUENT
ANALYSIS
FOR
OXIDA
TIVE
MODIFICATIONS
.............................................................................
71
3.3
DISCUSSION
.......................................................................................
73
4
FORMATION
OF
AGES
IN
SOD
KNOCKOUT
MICE
79
4.1
INTRODUCTION
AND
EXPERIMENTAL
DESIGN
........................................................
79
4.1.1
ANTIOXIDANT
STATUS
IN
SOD
MUTANT
MICE
...........................................
81
4.1.2
FETAL
FIBROBLASTS
FROM
MNSOD
MUTANT
MICE
.......................................82
4.2
RESULTS
...........................................................................................................
83
4.2.1
IN
VITRO
MODIFICATION
OF
DNA
BY
OXIDATIVE
STRESS
..............................
83
4.2.2
PREPARATION
OF
PRIMARY
MEFS
..........................................................
83
4.2.3
CULTURING
SOD2+/+
AND
SOD2
L+
MEFS
.............................................
84
4.2.4
DEVELOPMENT
OF
A
METHOD
FOR
THE
SIMULTANEOUS
ISOLATION
OF
NUCLEAR
DNA,
MTDNA,
AND
CYTOSOLIC
PROTEINS
FROM
PRIMARY
MEFS
86
4.2.5
ISOLATION
OF
NUCLEAR
DNA,
MTDNA
AND
CYTOSOLIC
PROTEINS
FROM
SOD2+/+AND
SOD2-/+FIBROBLASTS
.....................................................
87
4.2.6
CHARACTERIZATION
OF
THE
MITOCHONDRIAL
FRACTION
...............................
89
4.2.7
DETERMINATION
OF
CEDG
CONTENT
OF
NUCLEAR
DNA
AND
MTDNA
...
89
4.2.8
ANALYSIS
OF
PROTEIN
EXPRESSION
AND
AGE
MODIFICATIONS
.............
90
4.2.9
ANALYSIS
OF
OXIDATIVE
PROTEIN
EXPRESSION
..........................................
91
4.3
DISCUSSION
....................................................................................................
91
5
MATERIALS
AND
METHODS
97
5.1
INSTRUMENTATION
............................................................................................97
5.2
LABORATORY
EQUIPMENT
.................................................................................
98
5.3
CHEMICALS
AND
REAGENTS
...............................................................................99
5.4
METHODS
FOR
THE
IN
VITRO
ANALYSIS
OF
DNA
GLYCATION
PRODUCTS
...................
109
5.4.1
CULTIVATION
OF
HEK
293
T
CELLS
........................................................
109
5.4.2
PLASMID
DNA
PREPARATION
.............................................................
110
5.4.3
INTRODUCTION
OF
CEDG
MODIFICATIONS
.............................................
110
5.4.4
COMPETITIVE
ELISA
FOR
CEDG
...........................................................
112
5.4.5
TRANSIENT
TRANSFECTION
.....................................................................
112
5.4.6
REPORTER
GENE
ASSAY
........................................................................
112
5.4.7
CYTOTOXIC
EFFECT
ON
TRANSFECTED
HEK
293
T
CELLS
...........................
113
5.4.8
TRANSFORMATION
OF
GLYCATED
DNA
TO
A
BACTERIAL
HOST
......................
113
5.4.9
RESTRICTION
DIGESTION
OF
CEDG-MODIFIED
PLASMIDS
........................
113
5.4.10
RNA
ISOLATION
AND
RT-PCR
.............................................................
114
5.5
METHODS
FOR
THE
ANALYSIS
OF
ENDOGENOUSLY
FORMED
AGES
IN
NIH
3T3
FIBROBLASTS
.....................................................................................................
114
5.5.1
CULTIVATION
OF
NIH
3T3
MOUSE
EMBRYO
FIBROBLASTS
.........................
114
5.5.2
FREEZING
AND
THAWING
OF
NIH
3T3
FIBROBLASTS
.................................
115
5.5.3
SIMULTANEOUS
ISOLATION
OF
NUCLEI,
MITOCHONDRIA,
AND
CYTOSOLIC
PROTEINS
FROM
NIH
3T3
FIBROBLASTS
.................................................
115
5.5.4
ISOLATION
OF
DNA
FROM
NUCLEI
AND
MITOCHONDRIA
............................
116
5.5.5
ANALYSIS
OF
CELL
PROLIFERATION
...........................................................
116
5.5.6
ANALYSIS
OF
CELL
VIABILITY
...................................................................
117
5.5.7
SENESCENCE-LIKE
GROWTH
ARREST
OF
NIH
3T3
FIBROBLASTS
..................
117
5.5.8
ASSAY
FOR
SUCCINATE
DEHYDROGENASE
ACTIVITY
....................................
118
5.5.9
PCR
ANALYSIS
OF
THE
ISOLATED
MTDNA
..............................................
118
5.5.10
COMPETITIVE
ELISA
FOR
CEDG
............................................................
118
5.5.11
QUANTIFICATION
OF
PROTEIN
YIELD
.........................................................
119
5.5.12
GEL-ELECTROPHORETIC
SEPARATION
OF
CYTOSOLIC
PROTEINS
.......................
119
5.5.13
2-D
GEL
ELECTROPHORESIS
....................................................................
120
5.5.14
AGE-CML
ELISA
..............................................................................
122
5.5.15
ULTRAFILTRATION
....................................................................................
123
5.5.16
SYNTHESIS
OF
CML-MODIFIED
AND
CEL-MODIFIED
BSA
.......................
123
5.5.17
TEST
FOR
CML-ANTIBODY
SPECIFICITY
....................................................
123
5.5.18
WESTERN
BLOT
ANALYSIS
FOR
AGE-MODIFIED
PROTEINS
.........................
124
5.5.19
WESTERN
BLOT
FOR
ACTIN
......................................................................
125
5.5.20
WESTERN
BLOT
ANALYSIS
FOR
OXIDATIVE
PROTEIN
MODIFICATIONS
....
126
5.5.21
PARTIAL
ENZYMATIC
PROTEIN
HYDROLYSIS
IN
GEL
....................................
126
5.5.22
MALDI-TOF-MS
ANALYSIS
.................................................................
127
5.5.23
IMMUNOPRECIPITATION
OF
ACTIN
.........................................................
127
5.6
METHODS
FOR
THE
ANALYSIS
OF
AGES
IN
NUCLEAR
DNA,
MTDNA,
AND
CYTOSOLIC
PROTEINS
OF
SOD2
MEFS
..............................................................................
128
5.6.1
IN
VITRO
INCUBATION
OF
DNA
WITH
HYDROGEN
PEROXIDE
...................
128
5.6.2
BREEDING
AND
GENOTYPING
................................................................
129
5.6.3
PREPARATION
OF
PRIMARY
MOUSE
EMBRYONIC
FIBROBLASTS
(MEFS)
.
.
.
129
5.6.4
CULTIVATION
OF
MEFS
........................................................................
130
5.6.5
GROWTH
CURVES
OF
MEFS
..................................................................
130
5.6.6
SIMULTANEOUS
ISOLATION
OF
NUCLEI,
MITOCHONDRIA,
AND
CYTOSOLIC
PROTEINS
FROM
MEFS
.........................................................................
131
5.6.7
ISOLATION
OF
DNA
FROM
NUCLEI
AND
MITOCHONDRIA
...........................
132
5.6.8
ASSAY
FOR
SUCCINATE
DEHYDROGENASE
ACTIVITY
...................................
132
5.6.9
COMPETITIVE
ELISA
FOR
CEDG
..........................................................
133
5.6.10
WESTERN
BLOT
ANALYSIS
FOR
AGE-MODIFIED
PROTEINS
........................
133
5.6.11
WESTERN
BLOT
ANALYSIS
FOR
OXIDATIVE
PROTEIN
MODIFICATIONS
.
...
133
5.7
STATISTICAL
ANALYSIS
.........................................................................................
133
BIBLIOGRAPHY
135
LIST
OF
ABBREVIATIONS
153
LIST
OF
FIGURES
157
LIST
OF
TABLES
159
DANKSAGUNG
161
|
| any_adam_object | 1 |
| author | Breyer, Viola |
| author_GND | (DE-588)143994174 |
| author_facet | Breyer, Viola |
| author_role | aut |
| author_sort | Breyer, Viola |
| author_variant | v b vb |
| building | Verbundindex |
| bvnumber | BV037376840 |
| classification_rvk | WD 5360 |
| collection | ebook |
| ctrlnum | (OCoLC)725235920 (DE-599)DNB1011016532 |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV037376840 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T23:22:59Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-022530055 |
| oclc_num | 725235920 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-29T |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-29T |
| physical | V, 160 S. Ill., graph. Darst. 21 cm |
| psigel | ebook |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| record_format | marc |
| spelling | Breyer, Viola Verfasser (DE-588)143994174 aut Intracellular glycation of nuclear DNA, mitochondrial DNA, and cytosolic proteins triggered by endogenous oxidative stress = Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress vorgelegt von Viola Breyer Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress 2011 V, 160 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2011 Oxidativer Stress (DE-588)4353497-1 gnd rswk-swf DNS (DE-588)4070512-2 gnd rswk-swf Zellkern (DE-588)4067544-0 gnd rswk-swf Glykation (DE-588)4767145-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Oxidativer Stress (DE-588)4353497-1 s Zellkern (DE-588)4067544-0 s DNS (DE-588)4070512-2 s Glykation (DE-588)4767145-2 s b DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:29-opus-24465 https://open.fau.de/handle/openfau/1651 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-24465 Resolvingsystem http://d-nb.info/1011059290/34 Langzeitarchivierung Nationalbibliothek DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022530055&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Breyer, Viola Intracellular glycation of nuclear DNA, mitochondrial DNA, and cytosolic proteins triggered by endogenous oxidative stress = Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress Oxidativer Stress (DE-588)4353497-1 gnd DNS (DE-588)4070512-2 gnd Zellkern (DE-588)4067544-0 gnd Glykation (DE-588)4767145-2 gnd |
| subject_GND | (DE-588)4353497-1 (DE-588)4070512-2 (DE-588)4067544-0 (DE-588)4767145-2 (DE-588)4113937-9 |
| title | Intracellular glycation of nuclear DNA, mitochondrial DNA, and cytosolic proteins triggered by endogenous oxidative stress = Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress |
| title_alt | Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress |
| title_auth | Intracellular glycation of nuclear DNA, mitochondrial DNA, and cytosolic proteins triggered by endogenous oxidative stress = Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress |
| title_exact_search | Intracellular glycation of nuclear DNA, mitochondrial DNA, and cytosolic proteins triggered by endogenous oxidative stress = Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress |
| title_full | Intracellular glycation of nuclear DNA, mitochondrial DNA, and cytosolic proteins triggered by endogenous oxidative stress = Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress vorgelegt von Viola Breyer |
| title_fullStr | Intracellular glycation of nuclear DNA, mitochondrial DNA, and cytosolic proteins triggered by endogenous oxidative stress = Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress vorgelegt von Viola Breyer |
| title_full_unstemmed | Intracellular glycation of nuclear DNA, mitochondrial DNA, and cytosolic proteins triggered by endogenous oxidative stress = Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress vorgelegt von Viola Breyer |
| title_short | Intracellular glycation of nuclear DNA, mitochondrial DNA, and cytosolic proteins triggered by endogenous oxidative stress |
| title_sort | intracellular glycation of nuclear dna mitochondrial dna and cytosolic proteins triggered by endogenous oxidative stress intrazellulare glykierung genomischer dna mitochondrialer dna und zytosolischer proteine ausgelost durch endogenen oxidativen stress |
| title_sub | = Intrazelluläre Glykierung genomischer DNA, mitochondrialer DNA und zytosolischer Proteine ausgelöst durch endogenen oxidativen Stress |
| topic | Oxidativer Stress (DE-588)4353497-1 gnd DNS (DE-588)4070512-2 gnd Zellkern (DE-588)4067544-0 gnd Glykation (DE-588)4767145-2 gnd |
| topic_facet | Oxidativer Stress DNS Zellkern Glykation Hochschulschrift |
| url | https://open.fau.de/handle/openfau/1651 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-24465 http://d-nb.info/1011059290/34 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022530055&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT breyerviola intracellularglycationofnucleardnamitochondrialdnaandcytosolicproteinstriggeredbyendogenousoxidativestressintrazellulareglykierunggenomischerdnamitochondrialerdnaundzytosolischerproteineausgelostdurchendogenenoxidativenstress AT breyerviola intrazellulareglykierunggenomischerdnamitochondrialerdnaundzytosolischerproteineausgelostdurchendogenenoxidativenstress |