Advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation: development and applications
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
München
Verl. Dr. Hut
2011
|
| Edition: | 1. Aufl. |
| Series: | Physikalische Chemie
|
| Subjects: | |
| Online Access: | Inhaltstext Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-128621 Inhaltsverzeichnis |
| Physical Description: | 135 S. Ill., graph. Darst. 210 mm x 148 mm, 190 g |
| ISBN: | 9783868538649 |
Staff View
MARC
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| 245 | 1 | 0 | |a Advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation |b development and applications |c Matthias Höller |
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| 264 | 1 | |a München |b Verl. Dr. Hut |c 2011 | |
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Record in the Search Index
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IMAGE 1
CONTENTS
1 INTRODUCTION 1
2 FLUORESCENCE 3
2.1 FUNDAMENTALS 3
2.2 FLUORESCENCE ANISOTROPY 5
2.3 FOERSTER RESONANCE ENERGY TRANSFER 5
3 OPTICAL SYSTEM 7
3.1 CONFOCAL MICROSCOPY 7
3.2 THE CONFOCAL PRINCIPLE 7
3.3 PULSED INTERLEAVED EXCITATION 9
3.4 CONFOCAL SETUPS USED FOR THIS WORK 9
3.4.1 TWO-COLOR PIE MULTI-PARAMETER SETUP 10
3.4.2 THREE-COLOR PIE SCANNING SETUP 10
4 FLUORESCENCE FLUCTUATION SPECTROSCOPY 15
4.1 BASIC CONSIDERATIONS 15
4.2 NUMBER&BRIGHTNESS ANALYSIS . 16
4.2.1 DETECTOR NOISE CORRECTION 17
4.3 THE PHOTON COUNTING HISTOGRAM 19
4.3.1 THEORY OF PHOTON DETECTION: MANDEL'S FORMULA 19
4.3.2 FROM MANDEL TO NUMBER AND BRIGHTNESS 20
4.3.3 MULTIPLE FLUORESCENT SPECIES 23
4.4 FLUORESCENCE-INTENSITY DISTRIBUTION ANALYSIS 24
4.4.1 THE POINT SPREAD FUNCTION IN FIDA 24
4.4.2 THE GENERATING FUNCTION 26
4.5 FLUORESCENCE CUMULANT ANALYSIS 28
4.5.1 MOMENTS 28
4.5.2 FACTORIAL MOMENTS - 29
4.5.3 MOMENT GENERATING FUNCTION 29
4.5.4 FACTORIAL MOMENT GENERATING FUNCTION 30
4.5.5 CUMULANTS AND THE CGF 31
4.5.6 FACTORIAL CUMULANTS AND THE FCGF 32
4.5.7 DERIVATION OF FCA 32
4.6 FLUORESCENCE CORRELATION SPECTROSCOPY 35
4.6.1 THE AUTOCORRELATION FUNCTION 35
4.6.2 FLUORESCENCE INTENSITY TIME DEPENDENCE 37
4.6.3 MODEL FOR LINEAR REGRESSION 37
4.6.4 TRIPLET STATES 41
4.7 FLUORESCENCE CROSS-CORRELATION SPECTROSCOPY 42
4.7.1 THE CROSS-CORRELATION FUNCTION 42
I
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1011161494
DIGITALISIERT DURCH
IMAGE 2
4.8 SCANNING FCS 44
4.8.1 THE SCANNING CORRELATION CURVE 45
4.9 RASTER IMAGE CORRELATION SPECTROSCOPY 47
4.10 BURST ANALYSIS 48
4.10.1 BURST ANALYSIS WITH PIE 50
4.11 PHOTON DISTRIBUTION ANALYSIS 52
4.11.1 PDA THEORY 52
5 METHOD DEVELOPMENT WITH PIE 56
5.1 SCANNING FCS WITH PIE 56
5.2 PIE-SCANNING FCS IN AQUEOUS SOLUTION 57
5.3 RASTER IMAGE CORRELATION SPECTROSCOPY WITH PIE 60
5.4 PIE-RICS IN AQUEOUS SOLUTION 61
5.5 PIE-RICS IN LIVING CELLS 64
5.6 APPLICATION TO CAYL.4 CHANNEL DOMAIN 69
5.7 APPLICATION TO CAVL.4 CHANNEL ICDI SUBDOMAIN 71
5.8 DISCUSSION 73
6 DNMTL 75
6.1 INTRODUCTION 75
6.2 SAMPLE PREPARATION 76
6.3 EXCITATION POWER ANALYSIS 76
6.4 GFP/GBP 78
6.5 DNMTL:DNA COMPLEXES 82
6.6 DNMTL CROSS-CORRELATION 86
6.7 DNA CROSS-CORRELATION 88
6.8 FURTHER INVESTIGATION OF THE DNMTL:DNA BONDS 91
6.9 DISCUSSION 93
7 BURST ANALYSIS STUDY OF CONFORMATIONAL CHANGES OF BIP 96
7.1 BIP 96
7.2 SAMPLE PREPARATION 97
7.3 MONITORING BIP CONFORMATIONS 99
7.3.1 INTER-DOMAIN COMMUNICATION 101
7.3.2 CONFORMATION OF THE LID 102
7.4 THE CO-CHAPERONE ERDJ3 105
7.5 DISCUSSION 109
8 SUMMARY AND OUTLOOK 112
A SPECTRA 114
A.I TWO-COLOR PIE MULTI-PARAMETER SETUP 115
A.2 THREE-COLOR PIE SCANNING SETUP 116
A.2.1 DNMTL CONFIGURATION 116
A.2.2 RICS CONFIGURATION 118
B COMPUTER CODE 119
B.I NUMBER & BRIGHTNESS 119
B.2 FAST CORRELATION ALGORITHM [1] 120
II
IMAGE 3
B.3 FIT ALGORITHM FOR PCH AND FIDA 121
B.4 CUMULANT ANALYSIS 123
III |
| any_adam_object | 1 |
| author | Höller, Matthias 1979- |
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| bvnumber | BV037364130 |
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| dewey-full | 543.56 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 543 - Analytical chemistry |
| dewey-raw | 543.56 |
| dewey-search | 543.56 |
| dewey-sort | 3543.56 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| isbn | 9783868538649 |
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| physical | 135 S. Ill., graph. Darst. 210 mm x 148 mm, 190 g |
| psigel | ebook |
| publishDate | 2011 |
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| publisher | Verl. Dr. Hut |
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| series2 | Physikalische Chemie |
| spelling | Höller, Matthias 1979- Verfasser (DE-588)130377945 aut Advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation development and applications Matthias Höller 1. Aufl. München Verl. Dr. Hut 2011 135 S. Ill., graph. Darst. 210 mm x 148 mm, 190 g txt rdacontent n rdamedia nc rdacarrier Physikalische Chemie Zugl.: München, Univ., Diss., 2011 Fluoreszenzkorrelationsspektroskopie (DE-588)4636290-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Fluoreszenzkorrelationsspektroskopie (DE-588)4636290-3 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-128621 X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3716459&prov=M&dok_var=1&dok_ext=htm Verlag kostenfrei Inhaltstext http://edoc.ub.uni-muenchen.de/12862/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-128621 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022517612&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Höller, Matthias 1979- Advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation development and applications Fluoreszenzkorrelationsspektroskopie (DE-588)4636290-3 gnd |
| subject_GND | (DE-588)4636290-3 (DE-588)4113937-9 |
| title | Advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation development and applications |
| title_auth | Advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation development and applications |
| title_exact_search | Advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation development and applications |
| title_full | Advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation development and applications Matthias Höller |
| title_fullStr | Advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation development and applications Matthias Höller |
| title_full_unstemmed | Advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation development and applications Matthias Höller |
| title_short | Advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation |
| title_sort | advanced fluorescence fluctuation spectroscopy with pulsed interleaved excitation development and applications |
| title_sub | development and applications |
| topic | Fluoreszenzkorrelationsspektroskopie (DE-588)4636290-3 gnd |
| topic_facet | Fluoreszenzkorrelationsspektroskopie Hochschulschrift |
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