Mapping protein DNA interactions by cross-linking (Institut National de la Santé et de la Recherche Médicale, 2001)
Dissecting fission yeast centromeres via silencing -- Localizing replication sties and nuclear proteins -- Finding downstream target DNAs for chromatin proteins by X-CHIP in drosophila -- Emerging techniques: UV laser footprinting and protein-DNA crosslinking -- Nuclear organization and gene expression: visualization of transcription and higher order chromatin structure -- Examining the distribution of telomeric and DNA repair proteins by ChrIP and real-time PCR -- Nuclear organisation and gene silencing in lymphocytes -- Direct visualization of transcription factor binding to regulatory elements in living cells
Part I. Chromatin protein complexes: dynamic assemblies in the nucleus -- Isolation of homeodomain engrailed target genes by UV cross-linking and chromatin IP -- Analysis of chromatin assembly, chromatin domains, and DNA replication using xenopus systems -- Binding sights in chromatin by X-ChIP - Chromatin immunoprecipitation inbudding yeast -- ChIP with native chromatin: advantages and problems relative to methods using cross-linked material -- Highly sensitive FISH in combination with the visualization of the mammalian genome: architecture -- Part II. Fly-FISHing: a protocol to localize single copy genes inside the nucleus in whole mount embryos -- Part 2. Detailed method for immunoprecipitation of unfixed chromatin from mammalian cells