Holdings: Heterologous gene expression in E.coli

Heterologous gene expression in E.coli: methods and protocols

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Bibliographic Details
Other Authors:	Evans, Thomas C. (Editor)
Format:	Book
Language:	English
Published:	Totowa Humana Press 2011
Series:	Methods in molecular biology 705
Subjects:	Methode Escherichia coli Heterologe Genexpression Aufsatzsammlung
Online Access:	Inhaltstext Inhaltsverzeichnis
Physical Description:	XI, 310 S. Ill., graph. Darst.
ISBN:	9781617379666

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MARC


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adam_text	IMAGE 1 CONTENTS PREFACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . V CONTRIBUTORS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . IX 1. ADJUSTMENT OF CODON USAGE FREQUENCIES BY CODON HARMONIZATION IMPROVES PROTEIN EXPRESSION AND FOLDING . . . . . . . . . . . . . . . . . . . . . 1 EVELINA ANGOV, PATRICIA M. LEGLER, AND RYAN M. MEASE 2. SUMO FUSION TECHNOLOGY FOR ENHANCED PROTEIN EXPRESSION AND PURIFICATION IN PROKARYOTES AND EUKARYOTES . . . . . . . . . . . . . . . . . . 15 RAYMOND J. PEROUTKA III, STEVEN J. ORCUTT, JAMES E. STRICKLER, AND TAUSEEF R. BUTT 3. MOLECULAR AND CHEMICAL CHAPERONES FOR IMPROVING THE YIELDS OF SOLUBLE RECOMBINANT PROTEINS . . . . . . . . . . . . . . . . . . . . 31 ARIO DE MARCO 4. GENETIC SELECTION OF SOLUBILITY-ENHANCED PROTEINS USING THE TWIN-ARGININE TRANSLOCATION SYSTEM . . . . . . . . . . . . . . . . . . . . . 53 ADAM C. FISHER, MARK A. ROCCO, AND MATTHEW P. DELISA 5. PROTEIN FOLDING LIQUID CHROMATOGRAPHY . . . . . . . . . . . . . . . . . . . . . 69 QUAN BAI AND XINDU GENG 6. SITE-SPECIFIC PROTEIN LABELING BY INTEIN-MEDIATED PROTEIN LIGATION . . . . . . . 87 INCA GHOSH, NANCY CONSIDINE, ELISSA MAUNUS, LUO SUN, AIHUA ZHANG, JOHN BUSWELL, THOMAS C. EVANS, JR., AND MING-QUN XU 7. EFFICIENT EXPRESSION OF HUMAN AROMATASE (CYP19) IN E. COLI . . . . . . . . . . 109 NORIO KAGAWA 8. EXPRESSION OF RECOMBINANT CYTOCHROMES C IN E. COLI . . . . . . . . . . . . . . . 123 YURI Y. LONDER 9. SEMI-SYNTHESIS OF GLYCOPROTEINS FROM E. COLI THROUGH NATIVE CHEMICAL LIGATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151 JONATHAN P. RICHARDSON AND DEREK MACMILLAN 10. EXPRESSION OF RECOMBINANT PROTEINS WITH UNIFORM N-TERMINI . . . . . . . . . . 175 ORSOLYA KIRALY, LAN GUAN, AND MIKLOS SAHIN-TOTH 11. RECENT DEVELOPMENTS IN DIFFICULT PROTEIN EXPRESSION: A GUIDE TO E. COLI STRAINS, PROMOTERS, AND RELEVANT HOST MUTATIONS . . . . . . . . . . . . . . . . . 195 JAMES C. SAMUELSON 12. PERIPLASMIC CHAPERONES USED TO ENHANCE FUNCTIONAL SECRETION OF PROTEINS IN E. COLI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 MARTIN SCHLAPSCHY AND ARNE SKERRA VII IMAGE 2 VIII CONTENTS 13. ENGINEERING UNUSUAL AMINO ACIDS INTO PEPTIDES USING LANTIBIOTIC SYNTHETASE . . 225 JUN-ICHI NAGAO, KOUKI SHIOYA, YOSHITAKA HARADA, KEN-ICHI OKUDA, TAKESHI ZENDO, JIRO NAKAYAMA, AND KENJI SONOMOTO 14. THE TARGETED EXPRESSION OF NUCLEOTIDE SUGAR TRANSPORTERS TO THE E. COLI INNER MEMBRANE . . . . . . . . . . . . . . . . . . . . . . . . . . 237 JOE TIRALONGO AND ANDREA MAGGIONI 15. DETECTION OF PROTEIN-PROTEIN INTERACTIONS IN BACTERIA BY GFP-FRAGMENT RECONSTITUTION . . . . . . . . . . . . . . . . . . . . . . . . . 251 AKIRA KANNO, TAKEAKI OZAWA, AND YOSHIO UMEZAWA 16. ENHANCING THE SOLUBILITY OF RECOMBINANT PROTEINS IN ESCHERICHIA COLI BY USING HEXAHISTIDINE-TAGGED MALTOSE-BINDING PROTEIN AS A FUSION PARTNER . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 PING SUN, JOSEPH E. TROPEA, AND DAVID S. WAUGH 17. INTRODUCING PREDETERMINED MUTATIONS THROUGHOUT A TARGET GENE USING TDEM (TRANSPOSON-DIRECTED BASE-EXCHANGE MUTAGENESIS) . . . . . . . . 275 YUN CHEOL KIM 18. FLUORESCENT SITE-SPECIFIC LABELING OF ESCHERICHIA COLI EXPRESSED PROTEINS WITH SFP PHOSPHOPANTETHEINYL TRANSFERASE . . . . . . . . . . . . . . . . . . . . 295 AIHUA ZHANG, LUO SUN, JOHN BUSWELL, NANCY CONSIDINE, INCA GHOSH, ANASTASIYA MASHARINA, CHRISTOPHER NOREN, AND MING-QUN XU INDEX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 309
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discipline	Biologie Chemie-Ingenieurwesen Biotechnologie Medizin
format	Book
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isbn	9781617379666
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spelling	Heterologous gene expression in E.coli methods and protocols ed. by Thomas C. Evans ... Totowa Humana Press 2011 XI, 310 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 705 Methode (DE-588)4038971-6 gnd rswk-swf Escherichia coli (DE-588)4070959-0 gnd rswk-swf Heterologe Genexpression (DE-588)4342015-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Escherichia coli (DE-588)4070959-0 s Heterologe Genexpression (DE-588)4342015-1 s Methode (DE-588)4038971-6 s DE-604 Evans, Thomas C. edt Methods in molecular biology 705 (DE-604)BV035362695 705 X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3523267&prov=M&dok_var=1&dok_ext=htm Inhaltstext SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021071819&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Heterologous gene expression in E.coli methods and protocols Methods in molecular biology Methode (DE-588)4038971-6 gnd Escherichia coli (DE-588)4070959-0 gnd Heterologe Genexpression (DE-588)4342015-1 gnd
subject_GND	(DE-588)4038971-6 (DE-588)4070959-0 (DE-588)4342015-1 (DE-588)4143413-4
title	Heterologous gene expression in E.coli methods and protocols
title_auth	Heterologous gene expression in E.coli methods and protocols
title_exact_search	Heterologous gene expression in E.coli methods and protocols
title_full	Heterologous gene expression in E.coli methods and protocols ed. by Thomas C. Evans ...
title_fullStr	Heterologous gene expression in E.coli methods and protocols ed. by Thomas C. Evans ...
title_full_unstemmed	Heterologous gene expression in E.coli methods and protocols ed. by Thomas C. Evans ...
title_short	Heterologous gene expression in E.coli
title_sort	heterologous gene expression in e coli methods and protocols
title_sub	methods and protocols
topic	Methode (DE-588)4038971-6 gnd Escherichia coli (DE-588)4070959-0 gnd Heterologe Genexpression (DE-588)4342015-1 gnd
topic_facet	Methode Escherichia coli Heterologe Genexpression Aufsatzsammlung
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