Animal cell culture: essential methods
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Chichester [u.a.]
Wiley-Blackwell
2011
|
| Ausgabe: | 1. impression |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Hier auch später erschienene, unveränderte Nachdrucke |
| Beschreibung: | XXVII, 346 S. Ill., graph. Darst. |
| ISBN: | 9780470666586 |
Internformat
MARC
| LEADER | 00000nam a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV036960153 | ||
| 003 | DE-604 | ||
| 005 | 20171011 | ||
| 007 | t | ||
| 008 | 110117s2011 xxuad|| |||| 00||| eng d | ||
| 010 | |a 2010037008 | ||
| 020 | |a 9780470666586 |c pbk. |9 978-0-470-66658-6 | ||
| 035 | |a (OCoLC)706971498 | ||
| 035 | |a (DE-599)BVBBV036960153 | ||
| 040 | |a DE-604 |b ger |e aacr | ||
| 041 | 0 | |a eng | |
| 044 | |a xxu |c US | ||
| 049 | |a DE-20 |a DE-11 |a DE-355 |a DE-188 |a DE-19 |a DE-703 | ||
| 050 | 0 | |a QH585.2 | |
| 082 | 0 | |a 571.6/381 | |
| 084 | |a WX 6600 |0 (DE-625)152214:13423 |2 rvk | ||
| 084 | |a WX 6603 |0 (DE-625)152214:13427 |2 rvk | ||
| 245 | 1 | 0 | |a Animal cell culture |b essential methods |c ed.: John M. Davis |
| 250 | |a 1. impression | ||
| 264 | 1 | |a Chichester [u.a.] |b Wiley-Blackwell |c 2011 | |
| 300 | |a XXVII, 346 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Hier auch später erschienene, unveränderte Nachdrucke | ||
| 650 | 7 | |a cell culture |2 cabt | |
| 650 | 4 | |a Cell culture |x Technique | |
| 650 | 4 | |a Tissue culture |x Technique | |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Zellkultur |0 (DE-588)4067547-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Tiere |0 (DE-588)4060087-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Gewebekultur |0 (DE-588)4157245-2 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Tiere |0 (DE-588)4060087-7 |D s |
| 689 | 0 | 1 | |a Zellkultur |0 (DE-588)4067547-6 |D s |
| 689 | 0 | 2 | |a Gewebekultur |0 (DE-588)4157245-2 |D s |
| 689 | 0 | 3 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Davis, John M. |d 1952- |e Sonstige |0 (DE-588)1028057199 |4 oth | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020875051&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-020875051 | ||
Datensatz im Suchindex
| _version_ | 1804143685017796608 |
|---|---|
| adam_text | Contents
Contributors
xv
Preface
xvii
Abbreviations
xix
Protocols
xxv
I The Cell Culture Laboratory
1
Sue Clarke and Janette Dillon
1.1
Introduction
1
1.2
Methods and approaches
3
1.2.1
Cell culture laboratory design
3
1.2.1.1
Health and safety implications
4
1.2.1.2
The scale of the work
4
1.2.1.3
Segregation requirements
4
1.2.1.4
General considerations
5
1.2.1.5
Future needs
б
1.2.2
Services
6
1.2.2.1
Water
7
1.2.2.2
Pressurized gases
7
1.2.2.3
Liquid nitrogen
8
1.2.3
Equipment
8
1.2.3.1
Microbiological safety cabinet
8
1.2.3.2
Incubators
14
1.2.3.3
Centrifuges
16
1.2.3.4
Microscopes
16
1.2.3.5
Refrigerators and freezers
16
1.2.3.6
Liquid nitrogen refrigerators for cell storage
17
1.2.3.7
Miscellaneous equipment
17
1.2.4
Culture plasticware and associated small consumable items
18
1.2.4.1
Tissue culture plasticware
19
1.2.4.2
Miscellaneous small items
20
1.2.5
Washing reusable tissue culture equipment
21
1.2.5.1
Pipette washing
22
1.2.6
General care and maintenance of the tissue culture laboratory
23
1.3
Troubleshooting
25
1.3.1
Microbial contamination
26
viii
CONTENTS
1.3.1.1
Extent
1.3.1.2
Containment
1.3.1.3
Cleaning up
1.3.1.4
Identifying the source
1.3.1.5
Operator error
1.3.1.6
External sources
1.3.2
Quality
control testing
Acknowledgements
References
2
Sterilization
Peter
L
Roberts
2.1
Introduction
2.2
Methods and
aDDroaches
21
27
27
28
29
29
29
30
30
33
33
34
2.2.1
Wet heat
34
2.2.1.1
Autodaving
34
2.2.2
Oty
heat
41
2.2.2.1
Incineration
41
2.2.2.2
Hot-air ovens
41
2.2.3
Irradiation
43
2.2.3.1
Ultraviolet light
43
2.2.3.2
Gamma rays
43
2.2.4
Chemical sterilization
44
2.2.4.1
Fumigation
44
2.2.4.2
Liquid disinfectants
46
2.2.5
Filtration
48
2.2.5.1
Types of
filter
48
2.2.5.2
Types of filtration unit
48
2.2.5.3
Filtration set-up
49
2.2.5.4
Testing of bacteria! filters
50
2.2.5.5
HEPA filters
51
2.2.6
Viruses and
prions
53
2.2.6.1
Virus elimination
53
2.2.6.2
Filters for viruses
54
2.2.6.3
Prions
54
2.3
Troubleshooting
56
References
57
Microscopy of Living Cells
61
Colin Gray and Daniel
Zicha
3.1
Introduction
61
3.1.1
Chapter structure
61
3.1.2
Historical context
61
3.2
Methods and approaches
62
3.2.1
General imaging methodology
62
3.2.1.1
Magnification and resolution
64
3.2.1.2
Illumination
67
3.2.1.3
Contrast
70
CONTENTS ix
3.2.1.4
Aberrations
and their corrections
75
3.2.1.5
Environmental control for microscopy specimens
77
3.2.2
Imaging of intact cultured cells in flasks, dishes and plates
77
3.2.3
High-resolution imaging of cells on coverslips
78
3.2.3.1
Cell culture chambers
79
3.2.4
Imaging of fluorescently labelled cells
79
3.2.4.1
Fluorophores and cell labelling
80
3.2.4.2
Microscope configuration for fluorescence
80
3.2.4.3
Three-dimensional imaging
82
3.2.4.4
Direct imaging of protein dynamics and
specific molecular interactions
83
3.2.5
Recording of microscopy images
83
3.2.5.1
Time-lapse imaging
84
3.2.5.2
Multidimensional imaging
85
3.2.6
Analysis of microscopy images
86
3.2.6.1
Image enhancement
86
3.2.6.2
Image processing
-
extraction of morphometric data
86
3.2.6.3
Data analysis
-
statistical evaluation
87
3.2.7
Maintenance
87
3.3
Troubleshooting
88
Acknowledgements
88
References
88
Basic Techniques and Media, the Maintenance of Cell Lines,
and Safety
91
John M. Davis
4.1
Introduction
91
4.1.1
The terminology of cell and tissue culture
91
4.1.1.1
The origin of cell lines
92
4.1.1.2
The characteristics of normal and transformed cells
93
4.1.2
Basic components of the cell culture environment
95
4.1.2.1
Culture medium
96
4.1.2.2
Physicochemkal Factors
102
4.1.2.3
Stationary versus dynamic media supply
102
4.2
Methods and approaches
103
4.2.1
Sterile technique and contamination control
103
4.2.1.1
Working within the microbiological safety cabinet
103
4.2.1.2
Prevention of cellular cross-contamination
107
4.2.1.3
Cell culture at the open bench
108
4.2.2
General procedures for the cell culture laboratory
108
4.2.2.1
Maintenance of the laboratory
108
4.2.3
Preparation of culture media
112
4.2.4
The culture of attached cells
113
4.2.4.1
Routine culture substrates
114
4.2.4.2
Choice of culture vessels
116
4.2.4.3
Use of attachment factors and biosubstrates
118
4.2.4.4
Other culture substrates
121
4.2.5
In vitro cell growth behaviour
121
4.2.5.1
Adaptation to culture
121
4.2.5.2
Phases of cell growth
122
CONTENTS
4.2.6
Determinations of cell growth data
123
4.2.6.1
Calculation of in vitro age
123
4.2.6.2
Multiplication rate and population doubling time
125
4.2.6.3
Counting cells in suspension
126
4.2.6.4
Counting/quantifying cells adherent to a substrate
131
4.2.6.5
Expressions of culture efficiency
134
4.2.7
Transportation of cells
135
4.2.7.1
Transporting frozen cells
135
4.2.7.2
Transporting growing cells at ambient temperatures
137
4.2.8
Safety in the cell culture laboratory
141
4.2.8.1
Liquid nitrogen
141
4.2.8.2
Pathogens
142
4.3
Troubleshooting
144
Acknowledgements
146
References
146
5
Development and Optimization of Serum- and Protein-free
Culture Media
153
Stephen F. Gorfien and David W. Jayme
5.1
Introduction
153
5.1.1
Nomenclature definition
154
5.1.2
Eukaryotic cell culture applications
156
5.1.3
Impact of culture process and nutrient medium format
156
5.2
Methods and approaches
157
5.2.1
Preparation of medium
157
5.2.1.1
Use of liquid medium and supplemental additives
157
5.2.1.2
Reconstitution
of dry-format media
158
5.2.2
Nutrient optimization
-
determination of basal medium formulation
160
5.2.2.1
Analytical approaches to medium optimization
161
5.2.2.2
Metabolic profiling and statistical analysis as optimization tools
162
5.2.3
Combination of basal medium, nutrient feeds and other process parameters
163
5.2.4
Biological performance screening assays
171
5.2.4.1
Reproducibility
172
5.2.4.2
Scalability
172
5.2.4.3
Relevance
173
5.2.4.4
Sensitivity
173
5.2.4.5
Robustness
173
5.3
Troubleshooting
174
5.3.1
Media Preparation
-
What Can Go Wrong?
174
5.3.1.1
Storage
174
5.3.1.2
Solubilization
175
5.3.1.3
Sterilization
175
5.3.2
Generality versus specificity
-
Why cannot a single SFM work for every
cell application?
176
5.3.3
Sensitivity to environmental fluctuations
-
Why are my cells more
sensitive when cultured in SFM?
177
5.3.4
Adaptation of cells to SFM
-
How should I do this, and how and when
should banking occur to minimize
variabilit/?
178
5.4
Conclusion
180
CONTENTS Xi
Acknowledgements
181
References
181
6
Cryopreservation and Banking of Cell Lines
185
Glyn
Л/.
Stacey, Ross Hawkins and Roland A. Fleck
6.1
Introduction
185
6.2
Methods and approaches
187
6.2.1
Preparation for cryopreservation
188
6.2.2
Freezing and thawing of cells
193
6.2.3
Considerations for thawing cryopreserved material
200
6.2.4
Safety issues in cell banking
200
6.3
Troubleshooting
201
Acknowledgements
202
References
202
7
Primary Culture of Specific Cell Types and the Establishment
of Cell Lines
205
Kee
Woei
Ng, Mohan Chothirakottu Abraham, David
Tai
Wei Leong,
Chris Morris and
Jan-Thorsten Schantz
7.1
Introduction
205
7.2
Methods and approaches
206
7.2.1
Primary culture of mesenchymal cells (mesoderm)
207
7.2.1.1
Adipocytes
207
7.2.1.2
Chondrocytes
209
7.2.1.3
Dermal
fibroblasts
211
7.2.1.4
Osteoblasts
213
7.2.1.5
Umbilical vein endothelial cells
■ 216
7.2.2
Primary culture of ectodermal cells
218
7.2.2.1
Epithelium
218
7.2.3
Primary culture of endodermal cells
224
7.2.3.1
Hepatocytes
224
7.3
Troubleshooting
225
7.3.1
Cells
226
7.3.2
Materials
226
7.3.3
Culture environment
226
7.3.4
Protocols and techniques
229
References
229
б
Cloning
231
John Clarke, Alison Porter and John M. Davis
8.1
Introduction
231
8.1.1
Uses of cloning
231
8.1.2
Limitations of
doning
232
8.1.3
Special requirements of cells growing at low densities
234
xii CONTENTS
8.2
Methods and approaches 235
8.2.1
Development of techniques
235
8.2.2
Choice of technique 235
8.2.3
Methods applicable to both attached and suspension cells
236
8.2.3.1
Limiting dilution 236
8.2.3.2
Microspot
technique 24°
8.2.3.3
Cloning by micro-manipulation 241
8.2.3.4
Fluorescence-activated cell sorting
244
8.2.3.5
Automated cell cloning 244
8.2.4
Methods for attached cells 244
8.2.4.1
Cloning rings 244
8.2.4.2
Cloning on a hydrophilic FEP surface
246
8.2.5
Methods for suspension cells 248
8.2.5.1
Cloning in
soft agar
248
8.2.5.2
Cloning in methylcellulose 250
8.3
Troubleshooting 252
References 253
9
The Quality Control of Animal Cell Lines and the Prevention,
Detection and Cure of Contamination
255
Peter Thraves and Cathy Rowe
9.1
Introduction 255
9.2
Methods and approaches
256
9.2.1
Obtaining animal cell cultures
256
9.2.1.1
Cell culture collections
256
9.2.1.2
Quarantine and receipt of animal cell lines
256
9.2.2
Production of cell banks
259
9.2.3
Microbial quality control
259
9.2.3.1
Sources of contamination
260
9.2.3.2
Tests for bacteria, yeasts and fungi
260
9.2.3.3
Tests for mycoplasma
262
9.2.3.4
Virus testing
270
9.2.4
Transmissible spongiform encephalopathy
279
9.2.5
Eradication of contamination
279
9.2.6
Authentication
281
9.2.6.1
Iso-enzyme analysis
283
9.2.6.2
Cytogenetic analysis
284
9.2.6.3 DNA
fingerprinting
284
9.2.6.4
Short tandem repeat profiling
285
9.2.7
Regulatory aspects
287
9.2.8
Summary
289
9.3
Troubleshooting
289
9.3.1
Quality control considerations
290
9.3.1.1
Reagents and materials
290
9.3.1.2
Provenance and integrity of cell lines
290
9.3.1.3
Microbial contamination
291
9.3.2
Environmental monitoring
291
9.3.3
Good practice in the cell culture laboratory
291
References
292
CONTENTS xiii
10 Systems
for Cell Culture Scale-up
297
Jennifer Halsall and John M. Davis
10.1
Introduction
297
10.2
Methods and approaches
298
10.2.1
Adherent cells
298
10.2.1.1
Roller bottles
298
10.2.1.2
Stacked plates
301
10.2.1.3
Microcarriers
303
10.2.2
Suspension cells
307
10.2.2.1
Roller bottles
307
10.2.2.2
Shake flasks
308
10.2.2.3
Spinner flasks
309
10.2.2.4
Bioreactors
(fermenters) 310
10.2.2.5
Wave-type bioreactors
314
10.3
Troubleshooting
316
References
318
11
Good Laboratory Practice in the Cell Culture Laboratory
323
Barbara Orton
11.1
Introduction
323
11.1.1
What if...
323
11.1.2
Chapter aim
324
11.2
Background to GLP
325
11.3
General GLP principles
326
11.3.1
GLP objectives
326
11.3.2
Responsibilities
326
11.3.2.1
Management
326
11.3.2.2
Study Director and personnel
328
11.3.2.3
Quality Assurance personnel
328
11.3.2.4
Archive personnel
328
11.3.3
Training
328
11.3.4
Procedures
329
11.3.5
Records
329
11.3.6
Test item and test system
330
11.3.7
Facilities and equipment
331
11.3.8
Archives
331
11.3.9
QA and audits
333
11.4
Study performance
333
11.4.1
Planning and initiation
333
11.4.2
Study conduct
335
11.4.3
Study report
336
11.5
Good Manufacturing Practice
336
11.6
Summary
336
References
337
Index
339
Colour plates fall between pages
164
and
165.
|
| any_adam_object | 1 |
| author_GND | (DE-588)1028057199 |
| building | Verbundindex |
| bvnumber | BV036960153 |
| callnumber-first | Q - Science |
| callnumber-label | QH585 |
| callnumber-raw | QH585.2 |
| callnumber-search | QH585.2 |
| callnumber-sort | QH 3585.2 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WX 6600 WX 6603 |
| ctrlnum | (OCoLC)706971498 (DE-599)BVBBV036960153 |
| dewey-full | 571.6/381 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6/381 |
| dewey-search | 571.6/381 |
| dewey-sort | 3571.6 3381 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 1. impression |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01935nam a2200505zc 4500</leader><controlfield tag="001">BV036960153</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20171011 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">110117s2011 xxuad|| |||| 00||| eng d</controlfield><datafield tag="010" ind1=" " ind2=" "><subfield code="a">2010037008</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780470666586</subfield><subfield code="c">pbk.</subfield><subfield code="9">978-0-470-66658-6</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)706971498</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV036960153</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">aacr</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">xxu</subfield><subfield code="c">US</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-20</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-188</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-703</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH585.2</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">571.6/381</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WX 6600</subfield><subfield code="0">(DE-625)152214:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WX 6603</subfield><subfield code="0">(DE-625)152214:13427</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Animal cell culture</subfield><subfield code="b">essential methods</subfield><subfield code="c">ed.: John M. Davis</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1. impression</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Chichester [u.a.]</subfield><subfield code="b">Wiley-Blackwell</subfield><subfield code="c">2011</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XXVII, 346 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Hier auch später erschienene, unveränderte Nachdrucke</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">cell culture</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell culture</subfield><subfield code="x">Technique</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Tissue culture</subfield><subfield code="x">Technique</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Zellkultur</subfield><subfield code="0">(DE-588)4067547-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Tiere</subfield><subfield code="0">(DE-588)4060087-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Gewebekultur</subfield><subfield code="0">(DE-588)4157245-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Tiere</subfield><subfield code="0">(DE-588)4060087-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Zellkultur</subfield><subfield code="0">(DE-588)4067547-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Gewebekultur</subfield><subfield code="0">(DE-588)4157245-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Davis, John M.</subfield><subfield code="d">1952-</subfield><subfield code="e">Sonstige</subfield><subfield code="0">(DE-588)1028057199</subfield><subfield code="4">oth</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020875051&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-020875051</subfield></datafield></record></collection> |
| id | DE-604.BV036960153 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:51:35Z |
| institution | BVB |
| isbn | 9780470666586 |
| language | English |
| lccn | 2010037008 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020875051 |
| oclc_num | 706971498 |
| open_access_boolean | |
| owner | DE-20 DE-11 DE-355 DE-BY-UBR DE-188 DE-19 DE-BY-UBM DE-703 |
| owner_facet | DE-20 DE-11 DE-355 DE-BY-UBR DE-188 DE-19 DE-BY-UBM DE-703 |
| physical | XXVII, 346 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Wiley-Blackwell |
| record_format | marc |
| spelling | Animal cell culture essential methods ed.: John M. Davis 1. impression Chichester [u.a.] Wiley-Blackwell 2011 XXVII, 346 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Hier auch später erschienene, unveränderte Nachdrucke cell culture cabt Cell culture Technique Tissue culture Technique Methode (DE-588)4038971-6 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf Tiere (DE-588)4060087-7 gnd rswk-swf Gewebekultur (DE-588)4157245-2 gnd rswk-swf Tiere (DE-588)4060087-7 s Zellkultur (DE-588)4067547-6 s Gewebekultur (DE-588)4157245-2 s Methode (DE-588)4038971-6 s DE-604 Davis, John M. 1952- Sonstige (DE-588)1028057199 oth Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020875051&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Animal cell culture essential methods cell culture cabt Cell culture Technique Tissue culture Technique Methode (DE-588)4038971-6 gnd Zellkultur (DE-588)4067547-6 gnd Tiere (DE-588)4060087-7 gnd Gewebekultur (DE-588)4157245-2 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4067547-6 (DE-588)4060087-7 (DE-588)4157245-2 |
| title | Animal cell culture essential methods |
| title_auth | Animal cell culture essential methods |
| title_exact_search | Animal cell culture essential methods |
| title_full | Animal cell culture essential methods ed.: John M. Davis |
| title_fullStr | Animal cell culture essential methods ed.: John M. Davis |
| title_full_unstemmed | Animal cell culture essential methods ed.: John M. Davis |
| title_short | Animal cell culture |
| title_sort | animal cell culture essential methods |
| title_sub | essential methods |
| topic | cell culture cabt Cell culture Technique Tissue culture Technique Methode (DE-588)4038971-6 gnd Zellkultur (DE-588)4067547-6 gnd Tiere (DE-588)4060087-7 gnd Gewebekultur (DE-588)4157245-2 gnd |
| topic_facet | cell culture Cell culture Technique Tissue culture Technique Methode Zellkultur Tiere Gewebekultur |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020875051&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT davisjohnm animalcellcultureessentialmethods |