Quantitative thin-layer chromatography: a practical survey
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| Main Authors: | , , |
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| Format: | Book |
| Language: | English |
| Published: |
Heidelberg [u.a.]
Springer
2011
|
| Subjects: | |
| Online Access: | Inhaltstext Inhaltsverzeichnis |
| Physical Description: | XV, 388 S. Ill., graph. Darst. |
| ISBN: | 9783642107276 |
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MARC
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| 100 | 1 | |a Spangenberg, Bernd |d 1955- |e Verfasser |0 (DE-588)131662422 |4 aut | |
| 245 | 1 | 0 | |a Quantitative thin-layer chromatography |b a practical survey |c Bernd Spangenberg ; Colin F. Poole ; Christel Weins |
| 246 | 1 | 3 | |a Quantitative thin layer chromatography |
| 264 | 1 | |a Heidelberg [u.a.] |b Springer |c 2011 | |
| 300 | |a XV, 388 S. |b Ill., graph. Darst. | ||
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| 337 | |b n |2 rdamedia | ||
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| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Poole, Colin F. |e Verfasser |4 aut | |
| 700 | 1 | |a Weins, Christel |d 1951- |e Verfasser |0 (DE-588)143966383 |4 aut | |
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IMAGE 1
CONTENTS
1 HISTORY OF PLANAR CHROMATOGRAPHY 1
1.1 HISTORY OF PAPER CHROMATOGRAPHY (PC) 1
1.2 HISTORY OF THIN-LAYER CHROMATOGRAPHY 7
1.3 THE HISTORY OF QUANTITATIVE PLANAR CHROMATOGRAPHY 8
REFERENCES 10
2 THEORETICAL BASIS OF THIN LAYER CHROMATOGRAPHY (TLC) 13 2.1 PLANAR AND
COLUMN CHROMATOGRAPHY 13
2.2 TLC CAPILLARY FLOW 15
2.3 TLC DISTRIBUTION EQUILIBRIUM 18
2.3.1 ADSORPTION CHROMATOGRAPHY 18
2.3.2 PARTITION CHROMATOGRAPHY 20
2.4 THE RETARDATION FACTOR (R { ) 22
2.4.1 THE EMPIRICAL R F FACTOR 22
2.4.2 THE THERMODYNAMIC R' T FACTOR 24
2.5 MOBILE PHASE COMPOSITION 25
2.6 TRANSFER OF TLC SEPARATIONS TO COLUMNS 27
2.7 THE7? M VALUE 28
2.8 TEMPERATURE DEPENDENCE OF TLC SEPARATIONS 29
2.9 ADVANCED THEORETICAL CONSIDERATIONS 30
2.10 INDICES CHARACTERIZING SEPARATION AND RESOLUTION 37
2.11 ZONE BROADENING IN PLANAR CHROMATOGRAPHY 40
2.11.1 THE A TERM 40
2.11.2 THESSTERM 41
2.11.3 THE C TERM 42
2.11.4 LOCAL PLATE HEIGHT H 42
2.11.5 THE VAN DEEMTER EQUATION 43
2.12 OPTIMUM SEPARATION CONDITIONS IN TLC 45
2.13 SEPARATION NUMBER 47
2.14 REAL PLATE HEIGHT 50
REFERENCES 51
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1000038696
DIGITALISIERT DURCH
IMAGE 2
X CONTENTS
3 THE STATIONARY PHASE IN THIN-LAYER CHROMATOGRAPHY 53 3.1 ACTIVATING
AND DEACTIVATING STATIONARY PHASES 54
3.2 SNYDER'S ADSORPTION MODEL 55
3.3 LAYER CHARACTERISTICS 57
3.3.1 LAYER THICKNESS (D F ) 59
3.3.2 AVERAGE PARTICLE SIZE (D P ) 60
3.3.3 PARTICLE SIZE DISTRIBUTION 60
3.3.4 SPECIFIC SURFACE AREA (O S ) 60
3.3.5 PORE VOLUME (V P ) 61
3.3.6 AVERAGE PORE DIAMETER (P RF ) 61
3.4 THE MOST IMPORTANT STATIONARY PHASES IN TLC 62
3.4.1 ALUMINIUM OXIDE 62
3.4.2 MAGNESIUM SILICATE 62
3.4.3 SILICAGEL 63
3.4.4 CHEMICALLY BONDED SILICA GEL LAYERS 66
3.4.5 KIESELGUHR 67
3.4.6 CELLULOSE 68
3.4.7 POLYAMIDES 70
3.4.8 ION EXCHANGE RESINS 71
3.4.9 CHIRALPHASES 72
3.4.10 LAYERS WITH FLUORESCENT INDICATORS 73
3.4.11 MAKING YOUR OWN PLATES 73
3.5 LIGHT ABSORPTION ON PLATE SURFACES 74
REFERENCES 77
4 THE MOBILE PHASE IN ADSORPTION AND PARTITION CHROMATOGRAPHY 81 4.1
SOLVENT CHARACTERISTICS 81
4.2 SOLVENT THEORY FOR ADSORPTION CHROMATOGRAPHY (ACCORDING TO SNYDER)
83
4.2.1 SOLVENT STRENGTH (E) 85
4.2.2 SOLVENT STRENGTH OF BINARY MIXTURES 86
4.3 SOLVENT THEORY IN PARTITION CHROMATOGRAPHY 88
4.3.1 SOLVENT THEORY (ACCORDING TO SNYDER) 89
4.3.2 OTHER METHODS FOR CHARACTERIZING SOLVENTS 92
4.4 OPTIMIZING SOLVENT COMPOSITION 93
4.5 THE PRISMA MODEL (ACCORDING TO NYIREDY) 97
4.6 SOLVENT ADDITIVES 99
4.7 APPENDIX: SOLVENT PROPERTIES 102
REFERENCES 103
5 PREPARING AND APPLYING SAMPLES 105
5.1 SAMPLE PREPARATION 105
5.1.1 THE QUECHERS APPROACH 105
5.1.2 SOLID-PHASE EXTRACTION 106
5.1.3 STIR BAR SORPTIVE EXTRACTION 108
IMAGE 3
CONTENTS XI
5.2 THE DOSAGE QUALITY 108
5.3 CHOICE OF APPLICATION POSITION 112
5.4 PRACTICAL APPLICATION METHODS 113
5.4.1 SAMPLE APPLICATION VIA PLATE CONTACT 113
5.4.2 SAMPLE APPLICATION WITHOUT PLATE CONTACT 114
5.4.3 SAMPLE APPLICATION VIA CONTACT SPOTTING 114
5.4.4 PLATE OVERLOADING AND INCOMPLETE DRYING 116
REFERENCES 117
6 BASIS FOR TLC DEVELOPMENT TECHNIQUES 119
6.1 INFLUENCE OF THE VAPOUR PHASE 119
6.2 CHAMBER TYPES FOR LINEAR DEVELOPMENT 123
6.2.1 N-CHAMBERS ("TROUGH CHAMBERS") 123
6.2.2 S-CHAMBER ("SMALL CHAMBER") 124
6.2.3 VARIO-KS-CHAMBER 125
6.2.4 THE H-CHAMBER ("HORIZONTAL CHAMBER") 125
6.3 CONTROLLING SEPARATIONS VIA THE VAPOUR PHASE 126
6.3.1 SOLVENT COMPOSITION DURING SEPARATION 126
6.3.2 PLATE PRE-LOADING VIA THE VAPOUR PHASE 130
6.4 CIRCULAR SEPARATIONS 133
6.5 SOLVENT GRADIENTS 134
6.5.1 THEORY OF SOLVENT GRADIENTS 134
6.5.2 EVAPORATION-CONTROLLED GRADIENT ELUTION 140
6.5.3 MULTIPLE DEVELOPMENT IN TLC 142
6.5.4 AUTOMATED MULTIPLE DEVELOPMENT (AMD) 143
6.6 NORMAL PHASE SEPARATIONS WITH WATER-CONTAINING SOLVENTS 145 6.7
PLATE DEVELOPMENT WITH FORCED FLOW 147
6.7.1 ROTATION PLANAR CHROMATOGRAPHY (RPC) 147
6.7.2 OVER-PRESSURE LAYER CHROMATOGRAPHY (OPLC) 147 6.8 TWO DIMENSIONAL
TLC (2D TLC) 148
6.8.1 DEVELOPMENT IN ORTHOGONAL DIRECTIONS 148
6.8.2 GRAFTEDTLC 149
6.8.3 STABILITY TEST AND SRS TECHNIQUE 151
6.9 DRYING THE PLATE 153
REFERENCES 153
7 SPECIFIC STAINING REACTIONS 155
7. 1 CHEMICAL REACTIONS PRIOR TO SEPARATION (PRE-CHROMATOGRAPHIE
DERIVATIZATION) 157
7.1.1 SAMPLE ENRICHMENT BY PRE-CHROMATOGRAPHIC DERIVATIZATION 157
7.1.2 PRE-CHROMATOGRAPHIC IN SITU DERIVATIZATION 159
7.1.3 PRE-CHROMATOGRAPHIC STAINING 163
7.1.4 REAGENTS IN THE MOBILE PHASE 167
IMAGE 4
XII CONTENTS
7.2 POST-CHROMATOGRAPHIC REACTIONS (DERIVATIZATION AFTER DEVELOPMENT)
168
7.2.1 FLUORESCENCE ENHANCER 170
7.2.2 PH AND REDOX INDICATORS 171
7.2.3 UNIVERSAL REAGENTS (CHARRING REAGENTS) 172
7.2.4 ALDEHYDE REAGENTS 173
7.2.5 CH-AND NH-REACTING REAGENTS 176
7.2.6 BORON-CONTAINING REAGENTS 180
7.2.7 ALKALINE REAGENTS 182
7.2.8 CHLORAMINE-T REAGENT 183
7.2.9 DIAZOTIZATION REACTIONS 184
7.2.10 IODINE-STARCH AND WURSTERS REAGENTS 185
7.2.11 REACTIONS WITH METAL REAGENTS 187
7.2.12 REAGENTS FOR METAL CATIONS 190
7.3 REACTIONS VIA THE GAS PHASE 191
7.3.1 AMMONIUM BICARBONATE REAGENT 192
7.3.2 TIN(IV) CHLORIDE REAGENT 192
7.3.3 FORMIC ACID REAGENT 193
7.3.4 HYDROGEN CHLORIDE REAGENT 193
7.3.5 TRICHLOROACETIC ACID REAGENT 193
7.3.6 NITRIC ACID REAGENT 194
7.4 THERMAL TREATMENT OF TLC PLATES 194
7.5 ACTIVITY ANALYSIS USING CHEMICAL REAGENTS 194
7.5.1 FOLIN-CIOCALTEU REAGENT 195
7.5.2 CHECKING FOR FREE RADICAL SCAVENGER ACTIVITY USING DPPH REAGENT
195
7.5.3 NUCLEOPHILIC REACTION ABILITY 196
REFERENCES 197
8 BIOEFFECTIVE-LINKED ANALYSIS IN MODERN HPTLC 201
8.1 PRINCIPLE OF THE METHOD 202
8.1.1 CONTAMINANT ANALYSIS IN THE ENVIRONMENT AND FOOD AND THE PRINCIPLE
OF BIOACTIVITY-BASED ANALYSIS 202 8.1.2 AIMS AND FUNDAMENTAL ASPECTS OF
BIOEFFECTIVE-LINKED ANALYSIS BY THIN-LAYER CHROMATOGRAPHY 203
8.1.3 HPTLC AS A METHOD FOR BIOEFFECTIVE-LINKED ANALYSIS 203 8.2 GENERAL
RULES FOR THE ANALYSIS OF BIOEFFECTIVE COMPOUNDS 205 8.3 ENZYME TESTS
206
8.3.1 UREASE-INHIBITION TEST FOR HEAVY METALS 206
8.3.2 ANALYSIS USING REDOX ENZYMES 207
8.3.3 THE DETECTION OF CHOLINESTERASE INHIBITORS 208
8.4 INHIBITION OF PHOTOSYNTHESIS BY HERBICIDES 216
8.4.1 REAGENT PREPARATION 216
8.4.2 HILLREACTION 217
8.4.3 DETECTION USING ALGAE 217
IMAGE 5
CONTENTS XIII
8.5 DETECTING BIOEFFECTIVE COMPOUNDS WITH PHOTOBACTERIA 218 8.5.1
PRACTICAL USE OF PHOTOBACTERIA 218
8.5.2 REACTION TIME OPTIMIZATION 220
8.5.3 APPLICATIONS OF PHOTOBACTERIA 221
8.6 DETECTION OF FUNGICIDAL- AND ANTIBIOTICAL-ACTIVE SUBSTANCES IN
ENVIRONMENTAL SAMPLES 222
8.6.1 DETERMINING FUNGICIDES 222
8.6.2 SCREENING OF PESTICIDES IN FOOD AND SURFACE WATER 222 8.6.3
DETECTION OF COMPOUNDS BY ANTIBIOTIC ACTIVITY 223 8.7 YEAST ESTROGEN
SCREEN 225
REFERENCES 227
9 PLANAR CHROMATOGRAPHY DETECTORS 23 1
9.1 TRANSMITTANCE MEASUREMENTS IN THIN-LAYER CHROMATOGRAPHY 231 9.1.1
THE LAMBERT-BEER LAW 232
9.2 REFLECTANCE MEASUREMENTS IN TLC AND HPTLC 233
9.2.1 THE KUBELKA-MUNK EQUATION 234
9.2.2 REFLECTANCE MEASUREMENTS WITH A DIODE-ARRAY SCANNER 237 9.2.3
SPATIAL RESOLUTION ON THE PLATE 239
9.2.4 SPECTRAL DISTRIBUTION ON HPTLC PLATES 240
9.2.5 SPECTRAL EVALUATION ALGORITHM 242
9.2.6 VIDEO-DENSITOMETRIC MEASUREMENTS 245
9.3 INFRARED AND RAMAN DETECTION IN THIN-LAYER CHROMATOGRAPHY 247 9.3.1
ANALYSIS OF THIN-LAYER CHROMATOGRAMS BY DIFFUSE REFLECTANCE INFRARED
FOURIER TRANSFORMATION 247
9.3.2 ANALYSIS OF THIN-LAYER CHROMATOGRAMS BY NEAR-INFRARED FT-RAMAN
SPECTROSCOPY 248
9.3.3 ANALYSIS OF THIN-LAYER CHROMATOGRAMS BY SURFACE-ENHANCED RAMAN
SCATTERING SPECTROMETRY 249
9.4 MASS SPECTROMETRIC DETECTION IN TLC 250
9.4.1 DIRECT PLATE EXTRACTION (SSSP) 250
9.4.2 MALDI TECHNIQUES (MALDI-MS) 252
9.4.3 ATMOSPHERIC PRESSURE MASS SPECTROMETRY 252
9.5 THIN-LAYER RADIOCHROMATOGRAPHY (TL-RC) 254
9.5.1 DIRECT RADIOACTIVITY MEASUREMENTS ON TLC PLATES 254 9.5.2 PHOSPHOR
IMAGING 255
REFERENCES 257
10 DIFFUSE REFLECTANCE FROM TLC LAYERS 261
10.1 THE LAMBERT COSINE LAW 261
10.2 THEORY OF DIFFUSE REFLECTANCE 263
10.2.1 SPECIAL CASE A: THE REVERSAL REFLECTANCE FORMULA 267 10.2.2
SPECIAL CASE B: THE FLUORESCENCE FORMULA 267
10.2.3 SPECIAL CASE C: THE KUBELKA-MUNK EXPRESSION 268
IMAGE 6
XIV CONTENTS
10.3 MASS-DEPENDENT REFLECTION 270
10.4 SIMPLIFYING THE EXPRESSION 274
REFERENCES 274
11 FLUORESCENCE IN TLC LAYERS 277
11.1 THEORY OF FLUORESCENCE AND PHOSPHORESCENCE 277
11.2 FLUORESCENCE ENHANCEMENT 280
11.3 QUANTIFICATION IN TLC BY FLUORESCENCE 282
11.3.1 LOW SAMPLE CONCENTRATION FLUORESCENCE IN LIGHT SCATTERING MEDIA
283
11.3.2 HIGH SAMPLE CONCENTRATION FLUORESCENCE IN LIGHT SCATTERING MEDIA
284
11.4 CONTOUR PLOTS FOR FLUORESCENCE EVALUATION 285
11.5 TLC PLATES CONTAINING A FLUORESCENT DYE 285
REFERENCES 288
12 CHEMOMETRICS IN HPTLC 289
12.1 CALCULATION OF FL F VALUES 289
12.2 COMPOUND IDENTIFICATION USING UV-VISIBLE AND FLUORESCENCE SPECTRA
291
12.3 CORRELATION SPECTROSCOPY 293
12.3.1 THEORY OF CORRELATION SPECTROSCOPY 293
12.3.2 COMBINATION OF R F AND UV-VISIBLE SPECTRAL LIBRARY SEARCH 296
12.3.3 ZONE PURITY CHECK 296
12.4 SELECTION OF THE MEASUREMENT WAVELENGTH 298
12.5 STATISTICAL PHOTOMETRIC ERROR (DETECTOR VARIANCE) 301 12.5.1
RECIPROCAL MODEL 301
12.5.2 ABSORBANCE MODEL 302
12.5.3 KUBELKA-MUNK MODEL 303
12.5.4 FLUORESCENCE MODEL 304
12.5.5 MINIMIZING THE STATISTICAL PHOTOMETRIC ERROR 305 12.6 DIODE
BUNDLING AND DATA SMOOTHING 305
12.7 SIGNAL INTEGRATION: AREA OR HEIGHT EVALUATION? 307
12.8 DECONVOLUTION OF OVERLAPPING PEAKS 308
12.9 NEW VISUALIZATION METHODS FOR PLOTS 310
REFERENCES 313
13 STATISTICS FOR QUANTITATIVE TLC 315
13.1 THE MEAN VALUE 315
13.2 VARIANCE AND PRECISION 316
13.2.1 DEFINITION OF VARIANCE 316
13.2.2 RELATIVE VARIANCE 318
13.2.3 QUANTIFICATION OF RELATIVE VARIANCE 319
IMAGE 7
CONTENTS XV
13.3 TRUENESS, PRECISION, AND ACCURACY 320
13.4 THE GAUSS DISTRIBUTION 321
13.4.1 AREA OF THE GAUSS DISTRIBUTION 322
13.4.2 QUANTILES OF THE GAUSS DISTRIBUTION 322
13.4.3 TEST FOR A NORMAL DISTRIBUTION 324
13.5 STUDENT'S DISTRIBUTION (F DISTRIBUTION) 325
13.6 ERROR PROPAGATION 326
13.7 CALIBRATION METHODS 328
13.7.1 THE LINEAR CALIBRATION FUNCTION 329
13.7.2 ESTIMATING X^ 330
13.7.3 ESTIMATING A AND F^ 331
13.7.4 ESTIMATING THE VARIANCES OF A AND Y C 333
13.8 THEF-TEST 334
13.9 THE LINEAR REGRESSION VARIANCE 334
13.10 THE ANALYTICAL FUNCTION OF LINEAR REGRESSION 335
13.11 QUANTITATIVE ANALYSIS USING EXTERNAL STANDARDS 338 13.12
SECOND-ORDER CALIBRATION FUNCTION 339
13.13 ANALYTICAL FUNCTION OF THE SECOND-ORDER CALIBRATION 343 13.14 RISK
OF SYSTEMATIC ERROR 345
13.14.1 CONSTANT SYSTEMATIC ERROR 345
13.14.2 PROPORTIONAL SYSTEMATIC ERROR 345
13.15 USE OF INTERNAL STANDARDS 346
13.16 STANDARD ADDITION METHOD 347
13.17 MEANITEST 350
REFERENCES 351
14 PLANNING AN ANALYSIS AND VALIDATION IN TLC 353
14.1 TERMS USED IN VALIDATION 353
14.2 METHOD VALIDATION 354
14.2.1 TESTING SPECIFICITY 354
14.2.2 QUANTIFYING ANALYTES 357
14.2.3 GENERAL ASPECTS OF CALIBRATION 358
14.2.4 LINEARITY AND WORKING RANGE 359
14.2.5 CHOOSING A CALIBRATION FUNCTION 360
14.2.6 EXTERNAL STANDARD CALCULATION 362
14.2.7 OPTIMIZED CALIBRATION METHODS 362
14.2.8 PRECISION 363
14.2.9 ACCURACY 364
14.2.10 CONFIDENCE INTERVAL 367
14.2.11 LIMIT OF DETECTION AND LIMIT OF QUANTIFICATION 367 14.2.12
ROBUSTNESS 370
14.3 CONTROL CHARTS AS QUALITY INDICATORS IN ROUTINE ANALYSIS 372
REFERENCES 373
INDEX 375 |
| any_adam_object | 1 |
| author | Spangenberg, Bernd 1955- Poole, Colin F. Weins, Christel 1951- |
| author_GND | (DE-588)131662422 (DE-588)143966383 |
| author_facet | Spangenberg, Bernd 1955- Poole, Colin F. Weins, Christel 1951- |
| author_role | aut aut aut |
| author_sort | Spangenberg, Bernd 1955- |
| author_variant | b s bs c f p cf cfp c w cw |
| building | Verbundindex |
| bvnumber | BV036886145 |
| classification_rvk | VG 7300 |
| ctrlnum | (OCoLC)702640366 (DE-599)DNB1000038696 |
| dewey-full | 543.84 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 543 - Analytical chemistry |
| dewey-raw | 543.84 |
| dewey-search | 543.84 |
| dewey-sort | 3543.84 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie |
| format | Book |
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| id | DE-604.BV036886145 |
| illustrated | Illustrated |
| indexdate | 2024-07-20T10:54:35Z |
| institution | BVB |
| isbn | 9783642107276 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020801422 |
| oclc_num | 702640366 |
| open_access_boolean | |
| owner | DE-11 DE-19 DE-BY-UBM DE-92 DE-703 |
| owner_facet | DE-11 DE-19 DE-BY-UBM DE-92 DE-703 |
| physical | XV, 388 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Springer |
| record_format | marc |
| spelling | Spangenberg, Bernd 1955- Verfasser (DE-588)131662422 aut Quantitative thin-layer chromatography a practical survey Bernd Spangenberg ; Colin F. Poole ; Christel Weins Quantitative thin layer chromatography Heidelberg [u.a.] Springer 2011 XV, 388 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Quantitative Analyse (DE-588)4125311-5 gnd rswk-swf Dünnschichtchromatographie (DE-588)4013227-4 gnd rswk-swf Dünnschichtchromatographie (DE-588)4013227-4 s Quantitative Analyse (DE-588)4125311-5 s DE-604 Poole, Colin F. Verfasser aut Weins, Christel 1951- Verfasser (DE-588)143966383 aut Erscheint auch als Online-Ausgabe 978-3-642-10729-0 text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3424289&prov=M&dok_var=1&dok_ext=htm Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020801422&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Spangenberg, Bernd 1955- Poole, Colin F. Weins, Christel 1951- Quantitative thin-layer chromatography a practical survey Quantitative Analyse (DE-588)4125311-5 gnd Dünnschichtchromatographie (DE-588)4013227-4 gnd |
| subject_GND | (DE-588)4125311-5 (DE-588)4013227-4 |
| title | Quantitative thin-layer chromatography a practical survey |
| title_alt | Quantitative thin layer chromatography |
| title_auth | Quantitative thin-layer chromatography a practical survey |
| title_exact_search | Quantitative thin-layer chromatography a practical survey |
| title_full | Quantitative thin-layer chromatography a practical survey Bernd Spangenberg ; Colin F. Poole ; Christel Weins |
| title_fullStr | Quantitative thin-layer chromatography a practical survey Bernd Spangenberg ; Colin F. Poole ; Christel Weins |
| title_full_unstemmed | Quantitative thin-layer chromatography a practical survey Bernd Spangenberg ; Colin F. Poole ; Christel Weins |
| title_short | Quantitative thin-layer chromatography |
| title_sort | quantitative thin layer chromatography a practical survey |
| title_sub | a practical survey |
| topic | Quantitative Analyse (DE-588)4125311-5 gnd Dünnschichtchromatographie (DE-588)4013227-4 gnd |
| topic_facet | Quantitative Analyse Dünnschichtchromatographie |
| url | http://deposit.dnb.de/cgi-bin/dokserv?id=3424289&prov=M&dok_var=1&dok_ext=htm http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020801422&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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