PCR troubleshooting and optimization: the essential guide
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Norfolk
Caister Acad. Press
2011
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | VII, 235, 4 S. Ill., graph. Darst. |
| ISBN: | 9781904455721 1904455727 |
Staff View
MARC
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Record in the Search Index
| _version_ | 1804143586418098176 |
|---|---|
| adam_text | PCR Troubleshooting and
Optimization
The Essential Guide
Edited by
Suzanne Kennedy
MO BIO Laboratories, Inc ,
Carlsbad, CA, USA
and
Nick Oswald
BitesizeBio
Edinburgh, UK
Caister Academic Press
Contents
List of Contributors v
1 Magic in Solution: an Introduction and Brief History of PCR 1
Carl T Wittwer and Jared S Farrar
2 qPCR Inhibition and Amplification of Difficult Templates 23
Jack M Gallup
3 Significance of Controls and Standard Curves in PCR 67
Ian Kavanagh, Gerwyn Jones and Saima N Nayab
4 Obtaining Maximum PCR Sensitivity and Specificity 79
Cameron N Gundry and Matthew D Poulson
5 RT-PCR Optimization Strategies 97
Martina Reiter and Michael W Pfaffl
6 Real-Time PCR Instrumentation: an Instrument Selection Guide 119
Sandrine Javorschi-Miller and Ivan Delgado Orlic
7 Quantitative PCR Data Analysis - Unlocking the Secret
to Successful Results 139
Jan Hellemans and Jo Vandesompele
8 The MIQE Guidelines Uncloaked 151
Greg Shipley
9 PCR Applications for Epigenetics Research 167
Gavin Meredith, Miroslav Dudas, Mark Landers, Vasiliki Anest,
Jonathan Wang, Caifu Chen, Peter Jozsi and Christopher Adams
10 High-Resolution Melting Analysis 201
John F Mackay and Carl T Wittwer
11 Microfluidic Emulsion PCR 217
N Reginald Beer and John H Leamon
Index 233
Colour plates A1
|
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| classification_tum | CHE 860f CHE 810f BIO 040f BIO 180f |
| ctrlnum | (OCoLC)706089830 (DE-599)BVBBV036878745 |
| dewey-full | 572.86 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.86 |
| dewey-search | 572.86 |
| dewey-sort | 3572.86 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie |
| format | Book |
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| id | DE-604.BV036878745 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:50:01Z |
| institution | BVB |
| isbn | 9781904455721 1904455727 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020794188 |
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| physical | VII, 235, 4 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Caister Acad. Press |
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| spelling | PCR troubleshooting and optimization the essential guide ed. by Suzanne Kennedy ... Norfolk Caister Acad. Press 2011 VII, 235, 4 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier polymerase chain reaction cabt Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 s DE-604 Kennedy, Suzanne Sonstige oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020794188&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | PCR troubleshooting and optimization the essential guide polymerase chain reaction cabt Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| subject_GND | (DE-588)4256726-9 |
| title | PCR troubleshooting and optimization the essential guide |
| title_auth | PCR troubleshooting and optimization the essential guide |
| title_exact_search | PCR troubleshooting and optimization the essential guide |
| title_full | PCR troubleshooting and optimization the essential guide ed. by Suzanne Kennedy ... |
| title_fullStr | PCR troubleshooting and optimization the essential guide ed. by Suzanne Kennedy ... |
| title_full_unstemmed | PCR troubleshooting and optimization the essential guide ed. by Suzanne Kennedy ... |
| title_short | PCR troubleshooting and optimization |
| title_sort | pcr troubleshooting and optimization the essential guide |
| title_sub | the essential guide |
| topic | polymerase chain reaction cabt Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| topic_facet | polymerase chain reaction Polymerase-Kettenreaktion |
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