Microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Düsseldorf
VDI-Verl.
2010
|
| Edition: | Als Ms. gedr. |
| Series: | Fortschritt-Berichte VDI
Reihe 14, Landtechnik, Lebensmitteltechnik ; 134 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. Sprache |
| Physical Description: | VII, 146 S. Ill., graph. Darst. 21 cm |
| ISBN: | 9783183134144 |
Staff View
MARC
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| 100 | 1 | |a Heidebach, Thomas |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins |c Thomas Heidebach |
| 250 | |a Als Ms. gedr. | ||
| 264 | 1 | |a Düsseldorf |b VDI-Verl. |c 2010 | |
| 300 | |a VII, 146 S. |b Ill., graph. Darst. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
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| 490 | 1 | |a Fortschritt-Berichte VDI : Reihe 14, Landtechnik, Lebensmitteltechnik |v 134 | |
| 500 | |a Zsfassung in dt. Sprache | ||
| 502 | |a Zugl.: München, Techn. Univ., Diss., 2010 | ||
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| 650 | 0 | 7 | |a Mikroverkapselung |0 (DE-588)4169865-4 |2 gnd |9 rswk-swf |
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Record in the Search Index
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| adam_text | IMAGE 1
CONTENT
1. INTRODUCTION 1
2. LITERATURE REVIEW 3
2.1 MICROENCAPSULATION 3
2.2 ESTABLISHED METHODS FOR THE MICROENCAPSULATION OF MICROBIAL CELLS 6
2.2.1 EXTRUSION TECHNIQUE 7
2.2.2 EMULSION TECHNIQUE 9
2.2.3 SPRAY DRYING 10
2.3 ASSESSMENT OF CURRENT METHODS FOR PROBIOTIC ENCAPSULATION 11
2.3.1 ENCAPSULATION YIELD 11
2.3.2 THERAPEUTIC MINIMUM AND CORE LOAD 11
2.3.3 DESIRED CAPSULE-SIZES 12
2.3.4 CO-ENCAPSULATION OF PREBIOTIC SUBSTANCES 13
2.4 TECHNOLOGICAL CHALLENGES ARISING FROM PROBIOTIC ENCAPSULATION 13
2.4.1 HYDROCOLLOID-BASED MICROCAPSULES 13
2.4.2 PROTEIN-BASED MICROCAPSULES 15
2.4.2.1 TRANSGLUTAMINASE-INDUCED GELATION 16
2.4.2.2 RENNET INDUCED GELATION 17
2.4.3 LIPID-BASED MICROCAPSULES 18
2.5 IMPACT OF MICROCAPSULES ON PROBIOTIC SURVIVAL AND FOOD
CHARACTERISTICS 18
2.5.1 APPLICATION OF PROBIOTIC MICROCAPSULES IN YOGHURT 18
2.5.2 APPLICATION OF PROBIOTIC MICROCAPSULES IN CHEESE 21
2.5.3 OTHER FOODS CONTAINING ENCAPSULATED PROBIOTICS 22
2.5.4 PROTECTION DURING DRYING AND SUBSEQUENT DRIED STORAGE 25
2.5.4.1 DRYING OF MICROENCAPSULATED PROBIOTICS 25
2.5.4.2 STORAGE OF DRIED MICROCAPSULES 27
2.6 SURVIVAL DURING GASTRIC TRANSIT 28
2.6.1 ALGINATE-BASED MICROCAPSULES 29
2.6.2 OTHER POLYSACCHARIDE-BASED MATRIX-MATERIALS 34
2.6.3 PROTEIN BASED MICROCAPSULES 36
2.7 INTESTINAL SURVIVAL AND CELL RELEASE 38
2.7.1 PROTECTIVE EFFECT OF MICROENCAPSULATION UNDER SIMULATED INTESTINAL
CONDITIONS 38
2.7.2 RELEASE OF MICROENCAPSULATED CELLS IN THE INTESTINE 38
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/100898177X
DIGITALISIERT DURCH
IMAGE 2
VI
2.7.2.1 IN-VITRO INVESTIGATIONS 38
2.7.2.2 IN-VIVO INVESTIGATIONS 39
3. RATIONALE AND OBJECTIVES 41
4. MATERIALS AND METHODS 42
4.1 MATERIALS 42
4.2 CHEMICALS 44
4.3 ENCAPSULATION METHODS 44
4.3.1 ENCAPSULATION BASED ON TRANGLUTAMINASE-INDUCED GELATION OF SODIUM-
CASEINATE SUSPENSION 45
4.3.1.1 PREPARATION OF THE PROTEIN-CELL-MIXTURE 45
4.3.1.2 ENCAPSULATING PROCESS 45
4.3.2 ENCAPSULATION BASED ON RENNET-INDUCED GELATION OF
SKIM-MILK-CONCENTRATES
46
4.3.2.1 PREPARATION OF THE SKIM-MILK-CONCENTRATE-CELL-MIXTURE 46
4.3.2.2 ENCAPSULATION PROCESS 46
4.4 FREEZE-DRYING AND STORAGE OF DRIED POWDERS 47
4.5 STORAGE OF IN A MODEL MILK PRODUCT 48
4.6 ANALYTICAL PROCEDURES 48
4.6.1 VIABILITY OF FREE AND ENCAPSULATED BACTERIA DURING STORAGE IN
ACIDIFIED MILK.
48
4.6.2 VIABILITY OF FREE AND ENCAPSULATED BACTERIA AFTER SEQUENTIAL
INCUBATION AT
LOWPH-LEVELS 48
4.6.3 ENUMERATION OF THE FREE AND ENCAPSULATED BACTERIA 49
4.6.4 MECHANICAL DISINTEGRATION OF THE CAPSULES 49
4.6.5 ENZYMATIC DISINTEGRATION OF THE CAPSULES 49
4.6.6 DEGRADATION OF MICROCAPSULES DUE TO SIMULATED GASTRIC TREATMENT 50
4.6.7 RHEOLOGICAL MEASUREMENTS 50
4.6.8 DETERMINATION OF THE BUFFERING EFFECT OF THE MICROCAPSULES 50
4.6.9 CAPSULE SIZE DETERMINATION 51
4.6.10 OPTICAL EXAMINATION OF MICROCAPSULES 51
4.6.11 DETERMINATION OF THE PROTEIN CONTENT 52
4.6.12 MEASUREMENT OF WATER ACTIVITY AND WATER CONTENT 52
4.7 CALCULATION OF SURVIVAL RATES 52
IMAGE 3
VII
4.7.1 CALCULATION OF THE ENCAPSULATION YIELD AND SURVIVAL IN SIMULATED
GASTRIC
CONDITIONS 52
4.7.2 DETERMINATION OF SURVIVAL AFTER DRYING AND SUBSEQUENT STORAGE 53
4.8 STATISTICAL ANALYSES 54
5. RESULTS AND DISCUSSION 54
5.1 DEVELOPMENT OF AN ENCAPSULATION-METHOD BY MEANS OF TRANSGLUTAMINASE
54
5.1.1 GELATION BEHAVIOUR OF TRANSGLUTAMINASE INCUBATED CASEIN SOLUTIONS
54
5.1.2 FORMATION AND CHARACTERIZATION OF CASEIN CAPSULES 59
5.1.2.1 PARTICLE SIZE DISTRIBUTION 61
5.1.2.2 INFLUENCE OF THE ENCAPSULATION METHOD ON THE PROBIOTIC CELL
NUMBERS 66
5.2 DEVELOPMENT OF AN ENCAPSULATION-METHOD BY MEANS OF RENNET 68
5.2.1 GELATION BEHAVIOUR OF COLD-RENNETED SKIM-MILK-CONCENTRATE
SOLUTIONS 69
5.2.2 FORMATION AND CHARACTERIZATION OF MILK PROTEIN CAPSULES 72
5.2.3 INFLUENCE OF THE ENCAPSULATION METHOD ON PROBIOTIC CELL NUMBERS 76
5.3 ASSESSMENT OF THE DEVELOPED MICROCAPSULES 77
5.3.1 SURVIVAL UNDER SIMULATED GASTRIC CONDITIONS 77
5.3.1.1 TGASE-INDUCED MICROCAPSULES 78
5.3.1.2 INVESTIGATION OF THE PH-VALUE WITHIN THE CAPSULES 85
5.3.1.3 RENNET-INDUCED MICROCAPSULES 90
5.3.1.4 COMBINED APPLICATION OF TRANSGLUTAMINASE AND RENNET 94
5.4 INFLUENCE OF MICROENCAPSULATION ON STORAGE IN ACIDIFIED MILK 97
5.4.1 SURVIVAL DURING STORAGE 98
5.4.2 RELEASE OF CELLS FROM THE MICROCAPSULES DURING STORAGE 101
5.5 SURVIVAL DURING DRYING AND SUBSEQUENT DRIED STORAGE 104
5.5.1 SURVIVAL OF ENCAPSULATED PROBIOTIC CELLS DURING FREEZE DRYING 104
5.5.2 STORAGE OF DRIED MICROCAPSULES 108
5.5.2.1 INFLUENCE OF STORAGE TEMPERATURE AND RELATIVE HUMIDITY ON
SURVIVAL. 108
5.5.2.2 INFLUENCE OF MICROENCAPSULATION ON SURVIVAL DURING STORAGE 114
6. CONCLUSION 116
7. PERSPECTIVES: DEVELOPMENT OF NOVEL APPROACHES FOR PROBIOTIC
MICROENCAPSULATION
117
8. SUMMARY 122
9. ZUSAMMENFASSUNG 126
10. REFERENCES 131
|
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| indexdate | 2024-07-09T22:49:33Z |
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| isbn | 9783183134144 |
| language | English |
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| spelling | Heidebach, Thomas Verfasser aut Microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins Thomas Heidebach Als Ms. gedr. Düsseldorf VDI-Verl. 2010 VII, 146 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Fortschritt-Berichte VDI : Reihe 14, Landtechnik, Lebensmitteltechnik 134 Zsfassung in dt. Sprache Zugl.: München, Techn. Univ., Diss., 2010 Gelieren (DE-588)4156459-5 gnd rswk-swf Mikroverkapselung (DE-588)4169865-4 gnd rswk-swf Probiotikum (DE-588)4338112-1 gnd rswk-swf Enzymkatalyse (DE-588)4152480-9 gnd rswk-swf Milchproteine (DE-588)4134965-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Probiotikum (DE-588)4338112-1 s Mikroverkapselung (DE-588)4169865-4 s Milchproteine (DE-588)4134965-9 s Gelieren (DE-588)4156459-5 s Enzymkatalyse (DE-588)4152480-9 s DE-604 Fortschritt-Berichte VDI Reihe 14, Landtechnik, Lebensmitteltechnik ; 134 (DE-604)BV000021889 134 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020774830&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Heidebach, Thomas Microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins Fortschritt-Berichte VDI Gelieren (DE-588)4156459-5 gnd Mikroverkapselung (DE-588)4169865-4 gnd Probiotikum (DE-588)4338112-1 gnd Enzymkatalyse (DE-588)4152480-9 gnd Milchproteine (DE-588)4134965-9 gnd |
| subject_GND | (DE-588)4156459-5 (DE-588)4169865-4 (DE-588)4338112-1 (DE-588)4152480-9 (DE-588)4134965-9 (DE-588)4113937-9 |
| title | Microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins |
| title_auth | Microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins |
| title_exact_search | Microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins |
| title_full | Microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins Thomas Heidebach |
| title_fullStr | Microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins Thomas Heidebach |
| title_full_unstemmed | Microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins Thomas Heidebach |
| title_short | Microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins |
| title_sort | microencapsulation of probiotic microorganisms by means of enzymatic gelation of milk proteins |
| topic | Gelieren (DE-588)4156459-5 gnd Mikroverkapselung (DE-588)4169865-4 gnd Probiotikum (DE-588)4338112-1 gnd Enzymkatalyse (DE-588)4152480-9 gnd Milchproteine (DE-588)4134965-9 gnd |
| topic_facet | Gelieren Mikroverkapselung Probiotikum Enzymkatalyse Milchproteine Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020774830&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000021889 |
| work_keys_str_mv | AT heidebachthomas microencapsulationofprobioticmicroorganismsbymeansofenzymaticgelationofmilkproteins |