Investigation on developmental roles of the two zebrafish Claudin5 family members:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Göttingen
Cuvillier
2010
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | IX, 92 S. Ill., graph. Darst. |
| ISBN: | 9783869555461 |
Staff View
MARC
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Record in the Search Index
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|---|---|
| adam_text | IMAGE 1
CONTENTS
CONTENTS
ABSTRACT I
ZUSAMMENFASSUNG II
ABBREVIATIONS IV
CONTENTS VII
1. INTRODUCTIONS 1
1.1. CLAUDINS AS STRUCTURAL AND FUNCTIONAL COMPONENTS OF TIGHT JUNCTIONS
1 1.1.1. STRUCTURE AND CLASSES OF CLAUDINS 2
1.1.2. CLAUDINS IN TIGHTJUNCTION STRAND FORMATION 5
1.1.3. TIGHT JUNCTION ASSEMBLY AND EPITHELIAL CELL POLARITY FACTORS 6
1.1.4. CLAUDINS AND EMBRYONIC BARRIERS 8
1.2. ZEBRAFISH BRAIN VENTRICULAR EXPANSION 11
1.2.1. STEPS OF INITIAL ZEBRAFISH BRAIN VENTRICULAR EXPANSION 11
1.2.2. THE ROLE AND THE SECRETION OF CEREBROSPINAL FLUID IN BRAIN
VENTRICULAR
EXPANSION 12
1.2.3. THE ROLE OF NEUROEPITHELIUM IN BRAIN VENTRICLE FORMATION 14
13. VASCULOGENESIS OF THE ZEBRAFISH DORSAL AORTA AND CARDINAL VEIN 15
1.3.1. MORPHOGENESIS OF THE ZEBRAFISH DORSAL AORTA AND THE CARDINAL VEIN
15
1.3.2. REGULATION OF ARTERIAL-VENOUS SPECIFICATION 17
2. AIMS OF THIS STUDY 22
3. MATERIALS AND METHODS 23
3.1. MATERIALS 23
3.1.1. FISH LINES AND HUSBANDRY 23
3.1.2. ANTIBODIES, DYES, ENZYMES, CHEMICALS AND KITS 23
3.1.3. MEDIA, BUFFERS AND SOLUTIONS 25
3.1.4. IMPORTANT CHEMICALS 26
3.1.5. CONSUMABLES AND EQUIPMENTS 26
3.1.6. SOFTWARES 27
3.2. MOLECULAR BIOLOGY METHODS 28
- V I I-
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1008347094
DIGITALISIERT DURCH
IMAGE 2
CONTENTS
3.2.1. SENSE MRNA SYNTHESIS 28
3.2.2. SYNTHESIS OF ANTISENSE RNA PROBES 30
3.2.3. SITE DIRECT MUTAGENESIS CLONING 31
3 3. ANIMAL EXPERIMENTS 31
3.3.1. GENERATION OF INJECTION NEEDLES AND CHAMBERS 31
3.3.2. MORPHOLINO ANTISENSE OLIGONUCLEOTIDE DESIGN AND PREPARATION 31
3.3.3. EMBRYO INJECTION 32
3.3.4. ZEBRAFISH IMAGING AND BRAIN VENTRICLE INJECTION 32
3.3.5. SODIUM GREEN INDICATOR AND OUABAIN INCUBATION OF FISH EMBRYO 33
3.4. HISTOLOGY 33
3.4.1. VIBRATOM SECTIONING AND IMMUNOHISTOCHEMISTRY 33
3.4.2. WHOLE-MOUNT IN SITU HYBRIDIZATION 35
3.4.3. PLASTIC SECTION 36
3.4.4. EM ANALYSIS 37
3.5. SEQUENCE ANALYSIS AND PHYLOGENETIC ANALYSIS 37
4. RESULTS 39
4.1. IDENTIFICATION OF ZEBRAFISH CLAUDIN5 ORTHOLOGUES 39
4.1.1. IDENTIFICATION OF TWOZEBRAFLSH C/ARF/N5 GENES 39
4.1.2. ZEBRAFISH CLAUDIN5A IS EXPRESSED WITHIN THE BRAIN DURING
VENTRICULAR LUMEN
EXPANSION 40
4.2. CLAUDIN5A IS ESSENTIAL FOR ZEBRAFISH BRAIN VENTRICULAR LUMEN
EXPANSION 42
4.2.1. CLAUDIN5A CAN BE SPECIFICALLY KNOCKED-DOWN 42
4.2.2. LOSS OF CLAUDIN5A DOES NOT AFFECT THE TIGHT JUNCTION SCAFFOLD OF
THE
NEUROEPITHELIUM 44
4.2.3. LOSS OF CLAUDIN5A DISRUPTS THE NEUROEPITHELIAL-VENTRICULAR
PARACELLULAR
BARRIER FUNCTION 44
4.2.4. LOSS OF CLAUDIN5A AFFECTS BRAIN VENTRICULAR LUMEN EXPANSION AT 20
HPF 48
4.2.5. LOSS OF CLAUDIN5A DOES NOT INCREASE APOPTOTIC CELL DEATH OR
IMPAIR
PROLIFERATION RATES 50
4.2.6. LOSS OF CLAUDIN5A AFFECTS BRAIN VENTRICULAR LUMEN EXPANSION AT
LATER STAGE ... 51
4.2.7. CLAUDIN5A LOCALIZATION DEPENDS ON NEUROEPITHELIAL TISSUE
INTEGRITY 53
-VIII-
IMAGE 3
CONTENTS
4 3. CLAUDIN5A AND CLAUDIN5B ARE ESSENTIAL FOR ZEBRAFISH VASCULOGENESIS
56
4.3.1. CLAUDIN5A AND CLAUDIN5B ARE BOTH EXPRESSED WITHIN ENDOTHELIAL
VESSELS 56
4.3.2. LOSS OF CLAUDIN5A AND CLAUDIN5B AFFECTS THE SEPARATION OF DORSAL
AORTA AND
POSTERIOR CARDINAL VEIN 58
4.3.3. THE VASCULOGENESIS DEFECT CAUSED BY LOSS OF CLAUDINSA AND
CLAUDINSB CAN
BE RESCUED BY EITHER CLAUDIN5A OR CLAUDIN5A YI48A 59
4.3.4. LOSS OF CLAUDIN5A/B DOES NOT ALTER TIGHT JUNCTION FORMATION
BETWEEN
ENDOTHELIAL CELLS 60
4.3.5. LOSS OF CLAUDIN5AFT DOES NOT AFFECT ARTERIAL VERSUS VENOUS
DIFFERENTIATION .... 63
5. DISCUSSION 67
5.1. CLAUDIN5A TIGHTENS THE NEUROEPITHELIAL-VENTRICULAR BARRIER DURING
ZEBRAFISH BRAIN VENTRICULAR EXPANSION 67
5.2. NA +,K+-ATPASE AND CLAUDINSA CONTRIBUTE TO BRAIN VENTRICLE
EXPANSION VIA
SEPARATE PATHWAYS 69
53. THE ROLE OF CLAUDINSS IN ZEBRAFISH VASCULOGENESIS 72
BIBLIOGRAPHY 75
ACKNOWLEDGEMENTS 89
ERKLAERUNG 90
CURRICULUM VITAE 91
LIST OF PUBLICATIONS 92
- I X-
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| indexdate | 2024-07-09T22:48:40Z |
| institution | BVB |
| isbn | 9783869555461 |
| language | English |
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| physical | IX, 92 S. Ill., graph. Darst. |
| publishDate | 2010 |
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| publisher | Cuvillier |
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| spelling | Zhang, Jingjing 1981- Verfasser (DE-588)141961864 aut Investigation on developmental roles of the two zebrafish Claudin5 family members von Jingjing Zhang Göttingen Cuvillier 2010 IX, 92 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Berlin, Humboldt-Univ., Diss., 2010 Embryonalentwicklung (DE-588)4070792-1 gnd rswk-swf Zebrabärbling (DE-588)4079468-4 gnd rswk-swf Claudine Biologie (DE-588)7755373-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Zebrabärbling (DE-588)4079468-4 s Embryonalentwicklung (DE-588)4070792-1 s Claudine Biologie (DE-588)7755373-1 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020723432&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zhang, Jingjing 1981- Investigation on developmental roles of the two zebrafish Claudin5 family members Embryonalentwicklung (DE-588)4070792-1 gnd Zebrabärbling (DE-588)4079468-4 gnd Claudine Biologie (DE-588)7755373-1 gnd |
| subject_GND | (DE-588)4070792-1 (DE-588)4079468-4 (DE-588)7755373-1 (DE-588)4113937-9 |
| title | Investigation on developmental roles of the two zebrafish Claudin5 family members |
| title_auth | Investigation on developmental roles of the two zebrafish Claudin5 family members |
| title_exact_search | Investigation on developmental roles of the two zebrafish Claudin5 family members |
| title_full | Investigation on developmental roles of the two zebrafish Claudin5 family members von Jingjing Zhang |
| title_fullStr | Investigation on developmental roles of the two zebrafish Claudin5 family members von Jingjing Zhang |
| title_full_unstemmed | Investigation on developmental roles of the two zebrafish Claudin5 family members von Jingjing Zhang |
| title_short | Investigation on developmental roles of the two zebrafish Claudin5 family members |
| title_sort | investigation on developmental roles of the two zebrafish claudin5 family members |
| topic | Embryonalentwicklung (DE-588)4070792-1 gnd Zebrabärbling (DE-588)4079468-4 gnd Claudine Biologie (DE-588)7755373-1 gnd |
| topic_facet | Embryonalentwicklung Zebrabärbling Claudine Biologie Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020723432&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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