Immunohistochemistry: basics and methods
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| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Berlin ; Heidelberg
Springer
2010
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Literaturangaben |
| Physical Description: | X, 153 S. Ill. 24 cm |
| ISBN: | 9783642046087 |
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MARC
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Record in the Search Index
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|---|---|
| adam_text | CONTENTS 1 ANTIBODIES FOR IMMUNOHISTOCHEMISTRY 1 1.1 STRUCTURE OF
ANTIBODIES 2 1.2 POLYCLONAL ANTIBODIES 4 1.3 MOUSE MONOCLONAL ANTIBODIES
4 1.4 RABBIT MONOCLONAL ANTIBODIES 5 1.5 PROTEIN A AND PROTEIN G IN
IMMUNOHISTOCHEMISTRY 7 REFERENCES 8 2 ANTIBODY LABELING AND THE CHOICE
OF THE LABEL 9 2. 1 COVALENT LABELING OF ANTIBODIES 9 2.2 NON-COVALENT
LABELING OF PRIMARY ANTIBODIES WITH LABELED FAB FRAGMENTS 10 2.3 ENZYME
LABELS FOR LIGHT MICROSCOPY 13 2.4 FLUOROPHORE LABELS FOR FLUORESCENCE
MICROSCOPY 15 2.5 COLLOIDAL GOLD LABELS FOR ELECTRON MICROSCOPY 16
REFERENCES 17 3 PROBES PROCESSING IN IMMUNOHISTOCHEMISTRY 19 3.1
FIXATION IN IMMUNOHISTOCHEMISTRY 19 3.1.1 FIXATION IN ALCOHOLS AND
ACETONE 20 3.1.2 FIXATION IN FORMALDEHYDE 20 3.1.3 EFFECT OF
FORMALDEHYDE FIXATION ON ANTIGEN-ANTIBODY BINDING 21 3.2 PARAFFIN
SECTIONS FOR IMMUNOHISTOCHEMICAL ANALYSIS 22 3.2.1 EMBEDDING AND CUTTING
22 3.2.2 MOUNTING PARAFFIN SECTIONS ONTO SLIDES 23 3.3 CRYOSECTIONS FOR
IMMUNOHISTOCHEMICAL ANALYSIS 24 3.4 BUFFERS FOR WASHING AND ANTIBODY
DILUTION 25 3.5 MOUNTING FOLLOWING IMMUNOHISTOCHEMICAL STAINING 27 3.6
STORAGE FOLLOWING IMMUNOHISTOCHEMICAL STAINING 28 REFERENCES 28
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/998804940 DIGITALISIERT
DURCH VIII CONTENTS 4 WORKING WITH ANTIBODIES 31 4.1 DIRECT
IMMUNOSTAINING METHOD 31 4.2 INDIRECT IMMUNOSTAINING METHOD 33 4.3 THE
CHOICE OF ANTIBODIES 35 4.3.1 THE CHOICE OF PRIMARY ANTIBODIES 35 4.3.2
THE CHOICE OF SECONDARY ANTIBODIES 36 4.4 OPTIMAL CONCENTRATION OF THE
ANTIBODY 37 4.5 SPECIFICITY CONTROLS IN IMMUNOHISTOCHEMISTRY 38
REFERENCES 39 5 BACKGROUND STAINING, AUTOFLUORESCENCE AND BLOCKING STEPS
41 5.1 FC RECEPTORS 41 5.2 ENDOGENOUS PEROXIDASE 42 5.3 ENDOGENOUS
ALKALINE PHOSPHATASE 43 5.4 ENDOGENOUS BIOTIN 43 5.5 AUTOFLUORESCENCE OR
THE WOOD THROUGH THE TREES 44 REFERENCES 46 6 IMMUNOSTAINING
ENHANCEMENT 47 6. 1 ANTIGEN RETRIEVAL 47 6.1.1 HEAT-INDUCED ANTIGEN
RETRIEVAL 48 6.1.2 PROTEOLYTIC ANTIGEN RETRIEVAL 50 6.2 SIGNAL
AMPLIFICATION 51 6.2.1 AVIDIN-BIOTIN COMPLEX 52 6.2.2 CHAIN
POLYMER-CONJUGATED TECHNOLOGY 53 6.2.3 TYRAMIDE SIGNAL AMPLIFICATION 54
6.2.4 AMPLIFICATION OF THE AMPLIFIER 58 REFERENCES 58 7 MULTIPLE
MULTICOLOR IMMUNOENZYME STAINING 61 7.1 SIMULTANEOUS IMMUNOENZYMATIC
DOUBLE STAINING 61 7.2 SEQUENTIAL IMMUNOENZYMATIC DOUBLE/MULTIPLE
STAINING 63 7.3 STRIPPING BUFFERS FOR SEQUENTIAL IMMUNOENZYMATIC
DOUBLE STAINING 65 7.4 NUCLEAR COUNTERSTAINING FOLLOWING IMMUNOENZYME
LABELIN CONTENTS X 9 ANTIGEN DETECTION ON TISSUES USING PRIMARY
ANTIBODY RAISED IN THE SAME SPECIES 77 9.1 HAPTENYLATION OF PRIMARY
ANTIBODIES WITH THE FOLLOWING USE OF SECONDARY ANTIBODIES RECOGNIZING
THE CORRESPONDING HAPTEN 78 9.2 BLOCKING ENDOGENOUS TISSUE
IMMUNOGLOBULINS HOMOLOGOUS TO PRIMARY ANTIBODY BY PREINCUBATION WITH
UNCONJUGATED FAB FRAGMENTS 79 REFERENCES 81 10 PROBES FOR STAINING
SPECIFIC CELLULAR ORGANDIES 83 10.1 NUCLEAR MARKERS 83 10.1.1 NUCLEAR
FLUORESCENT COUNTERSTAINING 83 10.1.2 CELL-PROLIFERATION NUCLEAR MARKERS
85 10.1.3 NUCLEAR ENVELOPE MARKERS 85 10.1.4 NUCLEOLAR MARKERS 86 10.2
PROBES FOR MITOCHONDRIA 87 10.3 PROBES FOR ENDOPLASMIC RETICULUM AND
GOLGI APPARATUS 88 10.4 ENDOCYTIC PATHWAYS 89 10.5 PROBES FOR
CYTOSKELETON 91 10.6 PHALLOIDIN PROBES FOR ACTIN FILAMENTS 92 REFERENCES
94 11 THE USE OF EPITOPE TAGS IN HISTOCHEMISTRY 95 REFERENCES 98 12
IMMUNOHISTOCHEMISTRY AT THE ULTRASTRUCTURAL LEVEL 99 12.1 COLLOIDAL GOLD
CONJUGATES 99 12.2 FIXATION FOR ULTRASTRUCTURAL IMMUNOHISTOCHEMISTRY 100
12.3 RESIN ETCHING AND HEAT-INDUCED ANTIGEN UNMASKING IN RESIN SECTIONS
101 12.4 IMMUNOGOLD LABELING PROCEDURE 104 REFERENCES 107 13 DIAGNOSTIC
IMMUNOHISTOCHEMISTRY 109 13.1 KERATINS AND MYOEPITHELIAL MARKERS AS
DIAGNOSTIC MARKERS IN PROLIFERATIVE BREAST DISEASE AND IN TUMORS OF
BREAST 110 13.1.1 HUMAN KERATINS AND THEIR EXPRESSION PATTERNS I X
CONTENTS 13.1.7 KERATINS AND MYOEPITHELIAL MARKERS AS DIAGNOSTIC MARKERS
IN PROLIFERATIVE BREAST DISEASE AND IN TUMORS OF BREAST 117 13.1.8
CARCINOMAS OF LUMINAL PHENOTYPE (LUMINAL TYPE CARCINOMAS) IN THE BREAST
117 13.1.9 TUMORS OF BASAL PHENOTYPE 118 13.1.10 SQUAMOUS CELL
CARCINOMAS 119 13.2 TISSUE MICROARRAYS 122 REFERENCES 125 14 A PICTURE
IS WORTH A THOUSAND WORDS 129 14.1 BRIGHTFIELD MICROSCOPY 129 14.1.1 HOW
TO SET UP A LIGHT MICROSCOPE PROPERLY FOR TRANSMITTED LIGHT
ILLUMINATION: KOEHLER ILLUMINATION 130 14.1.2 THE CHOICE OF MICROSCOPE
OBJECTIVES 131 14.2 FLUORESCENCE MICROSCOPY 134 14.2.1 THE CHOICE OF
FILTER CUBES FOR FLUORESCENCE MICROSCOPY 134 14.2.2 THE CHOICE OF
FLUOROPHORES FOR MULTIPLE IMMUNOSTAINING 137 REFERENCES 139 GLOSSARY 141
INDEX 151
|
| any_adam_object | 1 |
| author | Buchvalov, Igor' B. Boecker, Werner |
| author_GND | (DE-588)14080384X |
| author_facet | Buchvalov, Igor' B. Boecker, Werner |
| author_role | aut aut |
| author_sort | Buchvalov, Igor' B. |
| author_variant | i b b ib ibb w b wb |
| building | Verbundindex |
| bvnumber | BV036739181 |
| classification_rvk | WC 5700 |
| ctrlnum | (OCoLC)699862456 (DE-599)DNB998804940 |
| dewey-full | 616.07 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.07 |
| dewey-search | 616.07 |
| dewey-sort | 3616.07 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Book |
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| illustrated | Illustrated |
| indexdate | 2024-07-09T22:46:59Z |
| institution | BVB |
| isbn | 9783642046087 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020656675 |
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| owner_facet | DE-355 DE-BY-UBR DE-29T |
| physical | X, 153 S. Ill. 24 cm |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | Springer |
| record_format | marc |
| spelling | Buchvalov, Igor' B. Verfasser (DE-588)14080384X aut Immunohistochemistry basics and methods Igor B. Buchwalow ; Werner Böcker Berlin ; Heidelberg Springer 2010 X, 153 S. Ill. 24 cm txt rdacontent n rdamedia nc rdacarrier Literaturangaben Immuncytochemie (DE-588)4161368-5 gnd rswk-swf Immuncytochemie (DE-588)4161368-5 s DE-604 Boecker, Werner Verfasser aut DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020656675&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Buchvalov, Igor' B. Boecker, Werner Immunohistochemistry basics and methods Immuncytochemie (DE-588)4161368-5 gnd |
| subject_GND | (DE-588)4161368-5 |
| title | Immunohistochemistry basics and methods |
| title_auth | Immunohistochemistry basics and methods |
| title_exact_search | Immunohistochemistry basics and methods |
| title_full | Immunohistochemistry basics and methods Igor B. Buchwalow ; Werner Böcker |
| title_fullStr | Immunohistochemistry basics and methods Igor B. Buchwalow ; Werner Böcker |
| title_full_unstemmed | Immunohistochemistry basics and methods Igor B. Buchwalow ; Werner Böcker |
| title_short | Immunohistochemistry |
| title_sort | immunohistochemistry basics and methods |
| title_sub | basics and methods |
| topic | Immuncytochemie (DE-588)4161368-5 gnd |
| topic_facet | Immuncytochemie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020656675&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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