Guide to techniques in mouse development: B Mouse molecular genetics
Saved in:
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier [u.a.]
2010
|
| Edition: | 2. ed. |
| Series: | Methods in enzymology
477 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XLVIII, 580 S., [4] Bl. Ill., graph. Darst. |
| ISBN: | 9780123848802 9780123848826 |
Staff View
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Record in the Search Index
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|---|---|
| adam_text | Contents
Contributors
xiii
Preface
xix
Volumes in Series
xxi
Section I.
Transgenesis 1
1.
Lentivirus
Transgenesis 3
Alexander
Pfeifer,
Tiongti Lim,
and
Katrin Zimmermann
1.
Introduction
4
2.
Generation of Lentiviral Vectors
5
3.
Generation of Transgenic Animals with Lentiviral Vectors
8
4.
Characterization of Transgenic Animals
11
5.
Summary
13
Acknowledgments
13
References
13
2.
GermUne Modification Using Mouse
Spermatogonial Stem Cells
17
Mito
Kanatsu-Shinohara and Takashi Shinohara
1.
Introduction
18
2.
Establishing and Maintaining a GS Cell Culture
19
3.
Gene Transduction and Genetic Selection of GS Cells
27
4.
Spermatogoniat Transplantation and Offspring Production
29
References
34
3.
Embryonic In Vivo Electroporation in the Mouse
37
Tetsuichiro
Saito
1.
Introduction
38
2.
Materials
40
3.
In
Utero
Electroporation
43
4.
Ехо
Utero
Electroporation
47
5.
Analysis of Electroporated Mice
49
Acknowledgments
49
References
49
vi
Contents
Section II. Transposons
51
4.
Current
Applications
of
Transposons
in Mouse Genetics
53
Adam J. Dupuy
1.
Introduction
54
2.
Molecular Characteristics of TEs with Activity in Mice
54
3.
Applications of TEs in Mouse Genetics
61
4.
The Future of TEs in Mouse Genetics
67
References
67
5.
Functional Genomics in the Mouse using the
Sleeping Beauty Transposon System
71
Kyoji Horie, Chikara Kokubu, and Junji Takeda
1.
Introduction
72
2.
Genome-Wide Germline Mutagenesis
with the SB Transgenic Approach
74
3.
Region-Specific Chromosome Engineering
with the SB Knock-in Approach
82
4.
Concluding Remarks
87
Acknowledgments
88
References
88
6.
The Use of
DNA
Transposons
for Cancer Gene
Discovery in Mice
91
George Vassiliou, Roland
Rad,
and Allan Bradley
1.
Introduction
92
2.
Choice of Transposon
93
3.
Transposon Design for Cancer Screens
95
4.
Whole-Body (Constitutive) Screens
98
5.
Tissue-Specific Mutagenesis
98
6.
Inducible Transposase Expression
100
7.
Mapping of Transposon Integration Sites
100
8.
Statistical Mining of Recurrent Integration Sites
101
9.
Validation of Putative Cancer Genes
101
10.
Idiosyncrasies of
Transposons as
Cancer
Gene Discovery Tools
102
11.
Concluding Remarks
103
References
103
Contents
vii
Section
III. Recombinases
107
7.
A
Practical Summary of Site-Specific Recombination,
Conditional Mutagenesis, and Tamoxifen
Induction of CreERT2
109
Konstantinos Anastassiadis,
Stefan
Glaser,
Andrea
Kranz,
Kaj
Berhardt, and A. Francis Stewart
1.
Introduction
110
2.
Recombinase
Target Sites
110
3.
Applications
112
4.
Alíele
Design
112
5.
Recombinase
Properties
114
6.
Tamoxifen Administration in Mice and Cultured Cells
117
7.
Problems
118
8.
Concluding Remarks
119
Acknowledgments
119
References
119
8.
A Recombineering Pipeline to Make Conditional
Targeting Constructs
125
Jun
Fu,
Madeleine Teucher,
Konstantinos
Anastassiadis,
William Skarnes, and A. Francis Stewart
1.
Introduction
126
2.
Recombineering
126
3.
Methods
127
4.
Standard Recombineering Electroporation Protocol
140
5.
Concluding Remarks
142
Acknowledgments
142
References
142
9.
Confirmation of Recombination Site Functionality
in Gene Targeting Vectors using
Recombinase-Expressing Bacteria
145
M. David Stewart and Richard R. Behringer
1.
Introduction
146
2.
Materials
147
3.
Method
147
4.
Example of Results
148
5.
Summary
150
Acknowledgments
150
References
150
viii Contents
10.
Genetic
Fate Mapping Using Site-Specific Recombinases
153
Emilie
Legué
and Alexandra
L
Joyner
1.
Principles Behind Genetic Fate Mapping
154
2.
Genetic Fate Mapping Technique
156
3.
Future Applications: Combining Genetic Fate
Mapping with Mutant Analysis
177
References
178
11.
Mapping Cell Fate and Function Using Recombinase-Based
Intersectional Strategies
183
Susan M. Dymecki, Russell S. Ray, and
Jun
С.
Kim
1.
Introduction
184
2.
Accessing Mouse Embryonic Cells In
Utero
for Tracer Molecule Delivery Using
Transgenesis
and Site-Specific
DNA
Recombination
185
3.
Improving Cell-Subtype Selectivity in Genetic Fate Maps
Using a Dual-Recombinase Intersectional Method
189
4.
Transgenes
Enabling Subtractive as well as Intersectional
Genetic Fate Mapping
193
5.
Exploiting Different Reporter Molecules to Reveal
Different Features of Mapped Cell Populations
195
6.
3forl
196
7.
Intersectional
Transgene
Activation Reaches
from Mapping Cell Fate to Mapping Cell Function
196
8.
Methods and Materials
199
9.
Concluding Remarks
208
Acknowledgments
209
References
209
Section IV. Mutagenesis
215
12.
Genome-Wide Forward Genetic Screens in Mouse
ES
Cells
217
Meng
Amy Li, Stephen J. Pettitt,
Kosuké Yusa,
and Allan Bradley
1.
Introduction
218
2.
Strategies for Genome-Wide Mutagenesis
220
3.
Using
ß/m-Deficient ES
Cells to Conduct
Recessive Genetic Screens
229
4.
Mutant Validation
232
5.
Concluding Remarks
238
Acknowledgments
239
References
240
Contents ix
13. Gene
Trap Mutagenesis in the Mouse
243
Rotand
H.
Friedel
and Philippe Soriano
1.
Introduction
244
2.
Gene Trapping Strategies
244
3.
Design of the Gene Trap Vector
253
4.
Gene Trapping Protocol for Retroviral Vectors
255
5.
Gene Trapping Protocol for Transposon Vectors
258
6.
Identification of Trap Insertion Sites
by Splinkerette PCR
259
7.
Ordering and Handling of Gene Trap
Clones from
Consortia
261
8.
Outlook
264
Acknowledgments
264
References
264
14.
A Wider Context for Gene Trap Mutagenesis
271
Joshua M. Brickman, Anestis Tsakiridis, Christine To,
and William
L
Stanford
1.
Introduction
272
2.
Gene Trap Vectors
273
3.
Random Integration as a Means to Generate New Mutations
278
4.
Targeted Trapping
279
5.
Public Resources of Mutagenized
ES
Cell Clones
280
6.
Gene Discovery and Annotation
285
7.
Hypothesis-Driven Screens
287
8.
Protocols
288
Acknowledgments
291
References
291
15.
Mouse Mutagenesis with the Chemical Supermutagen
ENU
297
Frank J. Probst and Monica
).
Justice
1.
Introduction
298
2.
Materials and Methods
304
3.
Conclusion
308
Acknowledgments
310
References
310
x
Contents
16.
Phenotype-Driven Mouse ENU
Mutagenesis
Screens
313
Tamara Caspary
1.
Introduction
314
2.
Screen
Design 315
3.
Screen Execution
316
4. Gene Identification 323
5.
The Future for Mouse Forward Genetics
326
References
326
17.
Using ENU Mutagenesis for Phenotype-Driven
Analysis of the Mouse
329
Rolf W. Stottmann and David R.
Beier
1.
Introduction
330
2.
ENU Screen Design
331
3.
ENU Treatment
336
4.
Mutant Ascertainment
339
5.
Mutation Identification
340
6.
Mutation Validation
343
7.
Summary
345
References
345
Section V. Gene Knockdowns
349
18.
Exploration of Self-Renewal and Pluripotency
in
ES
Cells Using RNAi
351
Christoph
Schaniel, Dung-Fang Lee, and Ihor R. Lemischka
1.
Introduction
352
2.
Maintenance of Mouse Embryonic Stem Cells
353
3.
siRNA-Mediated Gene Knockdown
354
4.
Lentivirus-Based shRNA Knockdown System
358
5.
Concluding Remarks
364
References
364
19.
Transgenic RNAi Applications in the Mouse
367
Jost Seibler and
Frieder Schwenk
1.
Introduction
368
2.
Short Interfering RNAs
369
3.
Short Hairpin
RNA
Expression Vectors
370
4.
Transgenic shRNA Expression In Vivo
371
5.
Conditional RNAi
373
6.
Experimental Protocols
376
References 382
Contents
x¡
20. Gene
Knockdown in the Mouse Through RNAi
387
Aljoscha
Kleinhammer,
Wolfgang
Wurst,
and
Ralf Kühn
1.
Introduction
388
2.
Principle of the Approach
389
3.
Materials
398
4.
Methods
399
5.
Concluding Remarks
412
Acknowledgments
413
References
413
21.
In Vivo Analysis of Gene Knockdown in
Tetracycline-lndudble shRNA Mice
415
Christopher S. Raymond, Lei Zhu, Thomas
F. Vogt,
and Myung K. Shin
1.
Introduction
416
2.
Methods
419
3.
Notes
426
References
426
22.
The Power of Reversibility: Regulating Gene
Activities via Tetracycline-Controlled Transcription
429
Kai Schönig,
Hermann Bujard, and Manfred
Gossen
1.
Introduction
430
2.
The
Tet
Regulatory Systems
431
3.
Tet-Transgenic Mice Available from Repositories
434
4.
Tet-Controlled
Transgenes
to Study
Learning and Memory
439
5.
Tet-Transgenic Animals in Cancer Research
439
6.
Secondary iPSC Technology
443
7.
Transgenic Rats
444
8.
Future Perspectives
444
References
447
Section VI. Gene Expression Profiling
455
23.
Gene Expression Profiling of Mouse Oocytes
and Preimplantation Embryos
457
Francesca E. Duncan and Richard M.
Schultz
1.
Introduction
458
2.
Experimental Design Considerations
463
xii Contents
3.
RNA
Isolation
and cRNA Target Preparation Protocol
(Based on the Affymetrix Protocol for Eukaryotic
Small Sample Target Labeling Assay Version II)
464
4.
Quality Control Assessment of cRNA
473
5.
Methods for Microarray Data Analysis
474
6.
Validation Methods
476
7.
Archiving Results
476
8.
Future Directions
476
Acknowledgments
478
References
479
24.
Interrogating the Transcriptome of Oocytes
and Preimplantation Embryos
481
Anne E. Peaston, Joel H.
Graber,
Barbara B. Knowles,
and Wilhelmine
N.
de Vries
1.
Introduction
482
2.
RNA
Relative Quantification in Oocytes and Preimplantation
Embryos
487
3.
Comparative Analysis of Large-Scale Expression Analyses
495
4.
Taking Isoform-Specific Changes into Account
500
5.
Concluding Remarks
502
Acknowledgments
503
References
506
25.
Gene Expression Profiling of Mouse
Embryos with Microarrays
511
Alexei A. Sharov, Yulan Piao, and
Minoru
S. H. Ko
1.
Introduction
512
2.
Considerations for Methods of Gene Expression Profiling
513
3.
Experimental Strategies
515
4.
Expression Profiling of Small Amounts of RNAs
520
5.
QC of Microarray Results
526
6.
Analysis and Interpretation of Microarray Data
531
7.
Submitting the Data to the Public Database
536
Acknowledgments
537
References
537
Author Index
543
Subject Index
571
|
| any_adam_object | 1 |
| author2 | Wassarman, Paul M. |
| author2_role | edt |
| author2_variant | p m w pm pmw |
| author_facet | Wassarman, Paul M. |
| building | Verbundindex |
| bvnumber | BV036728893 |
| classification_rvk | WC 4355 WG 1940 |
| ctrlnum | (OCoLC)705913835 (DE-599)BVBBV036728893 |
| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| indexdate | 2024-07-09T22:46:45Z |
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| isbn | 9780123848802 9780123848826 |
| language | English |
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| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Guide to techniques in mouse development B Mouse molecular genetics ed. by Paul M. Wassarman ... 2. ed. Amsterdam [u.a.] Elsevier [u.a.] 2010 XLVIII, 580 S., [4] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 477 Methods in enzymology ... Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Entwicklungsbiologie (DE-588)4152440-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Maus (DE-588)4169148-9 s Entwicklungsbiologie (DE-588)4152440-8 s Molekulargenetik (DE-588)4039987-4 s b DE-604 Wassarman, Paul M. edt (DE-604)BV036728888 2 Methods in enzymology 477 (DE-604)BV000000938 477 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020646607&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Guide to techniques in mouse development Methods in enzymology Molekulargenetik (DE-588)4039987-4 gnd Maus (DE-588)4169148-9 gnd Entwicklungsbiologie (DE-588)4152440-8 gnd |
| subject_GND | (DE-588)4039987-4 (DE-588)4169148-9 (DE-588)4152440-8 (DE-588)4143413-4 |
| title | Guide to techniques in mouse development |
| title_auth | Guide to techniques in mouse development |
| title_exact_search | Guide to techniques in mouse development |
| title_full | Guide to techniques in mouse development B Mouse molecular genetics ed. by Paul M. Wassarman ... |
| title_fullStr | Guide to techniques in mouse development B Mouse molecular genetics ed. by Paul M. Wassarman ... |
| title_full_unstemmed | Guide to techniques in mouse development B Mouse molecular genetics ed. by Paul M. Wassarman ... |
| title_short | Guide to techniques in mouse development |
| title_sort | guide to techniques in mouse development mouse molecular genetics |
| topic | Molekulargenetik (DE-588)4039987-4 gnd Maus (DE-588)4169148-9 gnd Entwicklungsbiologie (DE-588)4152440-8 gnd |
| topic_facet | Molekulargenetik Maus Entwicklungsbiologie Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020646607&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV036728888 (DE-604)BV000000938 |
| work_keys_str_mv | AT wassarmanpaulm guidetotechniquesinmousedevelopmentb |