Guide to techniques in mouse development: A Mice, embryos, and cells
Saved in:
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier [u.a.]
2010
|
| Edition: | 2. ed. |
| Series: | Methods in enzymology
476 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XLVIII, 482 S., [4] Bl. Ill., graph. Darst. |
| ISBN: | 9780123747754 9780123848833 |
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Record in the Search Index
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|---|---|
| adam_text | Contents
Contributors
xiii
Preface
xix
Volumes in Series
xxi
Section I. General Resources
1
1.
A Survey of Internet Resources for Mouse Development
3
Thomas L. Saunders
1.
Introduction
4
2.
Mouse Embryo Atlases
5
3.
Gene Expression Atlases and Databases
8
4.
Locating and Acquiring Mutant Mice and Embryonic Stem Cells
13
5.
Discussion
16
6.
Further Reading
17
References
17
Section II. Handling Mouse Lines
23
2.
Transport of Mouse Lines by Shipment of Live Embryos
25
Kevin A. Kelley
1.
Introduction
26
2.
Materials
28
3.
Methods
30
4.
Results and Discussion
33
References
35
3.
Strategies and Considerations for Distributing
and Recovering Mouse Lines
37
Yubin
Du, Wen
Xie, and Chengyu Liu
1.
Introduction
38
2.
Shipping Live Mice
39
3.
Shipping Nonfrozen Embryos or Gametes
42
v¡
Contents
4.
Archiving and Distributing Mouse Lines Through Cryopreservation
46
5.
Conclusion
49
Acknowledgment
50
References
50
4.
Archiving and Distributing Mouse Lines by Sperm
Cryopreservation,
IVF,
and Embryo Transfer
53
Hideko Takahashi and Chengyu Liu
1.
Introduction
54
2.
Sperm Cryopreservation
55
3.
Storing and Shipping Frozen Sperm Samples
59
4.
In Vitro Fertilization
61
5.
Oviduct Transfer
64
6.
Conclusions
67
Acknowledgment
68
References
68
Section III. Gametes and Embryos
71
5.
Isolation and Manipulation of Mouse Gametes and Embryos
73
Eveline S. Litscher and Paul M. Wassarman
1.
Introduction
74
2.
Materials, Media, and Solutions
74
3.
Collection and Storage Conditions
76
4.
Sperm-Egg Interactions
80
5.
Analysis of the Status of the Sperm s Acrosome
81
6.
Visualization of Gold-Labeled Antibody on Sperm
82
Acknowledgments
83
References
83
6.
Cryopreservation of Mouse Gametes and Embryos
85
Carlisle P.
Landei
1.
Introduction
86
2.
Cryopreservation of Mouse Embryos
86
3.
Cryopreservation of Spermatozoa
94
4.
Cryopreservation of Oocytes and Ovaries
100
5.
Troubleshooting 100
6.
Beyond the Methods: Other Considerations
101
7.
Summary
103
Acknowledgments
103
References
104
Contents
vii
7.
Ovarian Follicle Culture Systems for Mammals
107
David F.
Albertini
and
Gökhan Akkoyunlu
1.
Background
108
2.
2D Culture Systems
112
3. 3D
Culture Systems
115
4.
Considerations Based on Species Variations
117
5.
Conclusions and Future Considerations
118
Acknowledgments
119
References
119
8.
Production of Mouse Chimeras by Aggregating
Pluripotent Stem Cells with Embryos
123
Andras
Nagy,
Kristina
Nagy, and Marina Gertsenstein
1.
Introduction
124
2.
Methods for Producing Chimeras: General Issues
126
3.
Preparation of Donor Embryos
128
4.
Preparation of Pluripotent Cells
135
5.
Making the Aggregates
138
6.
Embryo Transfer to Pseudopregnant Females
142
7.
Detection of Chimerism and Testing for Germline Transmission
146
8.
Discussion
146
References
147
9.
Production of Cloned Mice from Somatic Cells,
ES
Cells,
and Frozen Bodies
151
Sayaka Wakayama, Eiji Mizutani, and Teruhiko Wakayama
1.
Introduction
152
2.
Equipment
153
3.
Mouse Strains
154
4.
Culture Media
154
5.
Chemicals
155
6.
Protocol
155
References
167
10.
Nuclear Transfer in Mouse Oocytes and Embryos
171
Zhiming Han, Yong Cheng, Cheng-Guang Liang, and Keith E. Latham
1.
Introduction
172
2.
Equipment and Solutions
172
3-
Procedures
175
Acknowledgments
184
References
184
viii
Contents
11.
Culture of Whole Mouse Embryos at Early
Postimplantation
to
Organogénesis
Stages: Developmental Staging and Methods
185
Jaime A. Rivera-Pérez,
Vanessa Jones, and Patrick P. L. Tarn
1.
Introduction
186
2.
Developmental Staging of
Postimplantation
Mouse
Embryos Dissected Between E5.5 and E8.25
188
3.
Materials
195
4.
Culture Protocols
198
Acknowledgments
202
References
202
12.
In
Utero
and
Ехо
Utero
Surgery on Rodent Embryos
205
Valérie Ngô-Muller
and Ken Muneoka
1.
Introduction
206
2.
Preparation for Abdominal Surgery
207
3.
In
Utero
Manipulation of Embryos
209
4.
Ехо
Utero
Manipulation of Embryos
212
5.
Postsurgery
215
6.
Experimental Approaches
216
7.
Summary
222
References
222
Section IV. Fertilization
227
13.
Enhancement of
IVF
in the Mouse by Zona-Drilling
229
Kevin A. Kelley
1.
Introduction
230
2.
Materials
233
3.
Methods
235
4.
Results and Discussion
240
References
249
14.
ICS!
in the Mouse
251
Paula Stein and Richard M.
Schultz
1.
Introduction
252
2.
Experimental Procedures
252
Acknowledgments
261
References
261
Contents ix
Section
V.
ES
and IPS Cells
263
15.
A Simple Procedure for the Efficient Derivation
of Mouse
ES
Cells
265
Esther S. M. Wong, Kenneth H. Ban, Rafidah Mutalif, Nancy A. Jenkins,
Neal G. Copeland, and Colin
L
Stewart
1.
Introduction
266
2.
Methodology/Workflow
269
3.
Concluding Remarks and Future Perspective
281
References
282
16.
Producing Fully
ES
Cell-Derived Mice from Eight-Cell Stage
Embryo Injections
285
Thomas M. DeChiara, William T. Poueymirou,
Wojtek Auerbach,
David Frendewey, George D. Yancopoulos, and David M. Valenzueta
1.
Introduction
286
2.
Methods
288
3.
Summary
293
References
294
17.
The Loss-of-Allele Assay for
ES
Cell Screening and
Mouse Genotyping
295
David Frendewey,
Rostislav Chemomorsky,
Lakeisha Esau,
Jinsop Om, Yingzi Xue, Andrew J. Murphy, George D. Yancopoulos,
and David M. Valenzuela
1.
Introduction
296
2.
Methods
298
References
307
18.
Induced Pluripotent Stem Cells
309
Holm Zaehres, Jeong Beom Kim, and Hans R.
Schöler
1.
Generation of iPS Cells
309
2.
Expression Vectors for iPS Cell Generation
311
3.
Retrovirus Production
314
4.
iPS Cell Generation with Retroviral Vectors
315
5.
iPS Cell Generation with Proteins and Small Molecules
317
6.
iPS Cell Characterization
319
7.
Biotechnological and Medical Applications
321
Acknowledgments
321
References
322
x
Contents
Section
VI. Imaging Mouse Development
327
19.
Imaging Mouse Embryonic Development
329
Ryan S. Udan and Mary E. Dickinson
1.
Introduction
330
2.
Overview of Key Parameters
331
3.
Whole Embryo Culture
333
4.
Labeling Cells of Interest
338
5.
Time-Lapse Imaging of Early Mouse Embryos
340
6.
Image Analysis
344
7.
Summary
346
References
346
20.
Imaging Mouse Development with Confocal
Time-Lapse Microscopy
351
Sonja
Nowotschin, Anna Ferrer-Vaquer, and Anna-Katerina Hadjantonakis
1.
Introduction
352
2.
Materials
355
3.
Methods
361
Acknowledgments
375
References
376
21.
Ultrasound and Magnetic Resonance Microimaging of
Mouse Development
379
Brian J. Nieman and Daniel H.
Turnbull
1.
Introduction
380
2.
Ultrasound Biomicroscopy
381
3.
Magnetic Resonance Microimaging
385
4.
Summary
396
Acknowledgments
396
References
397
Section
VII.
Hematopoiesis
401
22.
Use of Transgenic Fluorescent Reporter Mouse Lines
to Monitor Hematopoietic and Erythroid Development
During
Embryogenesis 403
Stuart
T. Fraser,
Joan Isern, and Margaret H. Baron
1.
Introduction
404
2.
Materials
410
Contents xi
3.
Methods
413
4. Notes 423
References
423
23. Identification
and
In
Vivo Analysis of
Murine
Hematopoietic
Stem Cells
429
Serine
Avagyan, Yacine M. Amrani, and Hans-Willem Snoeck
1.
Hematopoietic Stem Cells
429
2.
Assays of Hematopoietic Stem Cells
431
3.
Isolation of Hematopoietic Stem Cells
438
References
442
Author Index
449
Subject Index
473
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| spelling | Guide to techniques in mouse development A Mice, embryos, and cells ed. by Paul M. Wassarman ... 2. ed. Amsterdam [u.a.] Elsevier [u.a.] 2010 XLVIII, 482 S., [4] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 476 Methods in enzymology ... Zellkultur (DE-588)4067547-6 gnd rswk-swf Entwicklungsbiologie (DE-588)4152440-8 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Maus (DE-588)4169148-9 s Entwicklungsbiologie (DE-588)4152440-8 s Zellkultur (DE-588)4067547-6 s b DE-604 Wassarman, Paul M. edt (DE-604)BV036728888 1 Methods in enzymology 476 (DE-604)BV000000938 476 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020646604&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Guide to techniques in mouse development Methods in enzymology Zellkultur (DE-588)4067547-6 gnd Entwicklungsbiologie (DE-588)4152440-8 gnd Maus (DE-588)4169148-9 gnd |
| subject_GND | (DE-588)4067547-6 (DE-588)4152440-8 (DE-588)4169148-9 (DE-588)4143413-4 |
| title | Guide to techniques in mouse development |
| title_auth | Guide to techniques in mouse development |
| title_exact_search | Guide to techniques in mouse development |
| title_full | Guide to techniques in mouse development A Mice, embryos, and cells ed. by Paul M. Wassarman ... |
| title_fullStr | Guide to techniques in mouse development A Mice, embryos, and cells ed. by Paul M. Wassarman ... |
| title_full_unstemmed | Guide to techniques in mouse development A Mice, embryos, and cells ed. by Paul M. Wassarman ... |
| title_short | Guide to techniques in mouse development |
| title_sort | guide to techniques in mouse development mice embryos and cells |
| topic | Zellkultur (DE-588)4067547-6 gnd Entwicklungsbiologie (DE-588)4152440-8 gnd Maus (DE-588)4169148-9 gnd |
| topic_facet | Zellkultur Entwicklungsbiologie Maus Aufsatzsammlung |
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