Techniques in confocal microscopy:
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
London [u.a.]
Acad. Press
2010
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| Series: | Reliable lab solutions
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XXI, 511 S., [9] Bl. Ill. |
| ISBN: | 0123846587 9780123846587 |
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| adam_text | TECHNIQUES IN CONFOCAL MICROSCOPY RELIABLE LAB SOLUTIONS EDITED BY P.
MICHAEL CONN DIRECTOR, OFFICE OF RESEARCH ADVOCACY (OHSU) SENIOR
SCIENTIST, DIVISIONS OF REPRODUCTIVE SCIENCES AND NEUROSCIENCE (ONPRC)
PROFESSOR, DEPARTMENTS OF PHARMACOLOGY AND PHYSIOLOGY, CELL AND
DEVELOPMENTAL BIOLOGY, AND OBSTETRICS AND GYNECOIOGY (OHSU) BEAVERTON,
OREGON AMSTERDAM * BOSTON * HEIDELBERG * LONDON NEW YORK * OXFORD *
PARIS * SAN DIEGO SAN FRANCISCO * SINGAPORE * SYDNEY * TOKYO X^L^XI V
1-EJA. ACADEMIC PRESS IS AN IMPRINT OF ELSEVIER CONTENTS CONTRIBUTORS
PREFACE PART I PRACTICAL CONSIDERATIONS AND EQUIPMENT 1. THEORETICAL
BASIS OF CONFOCAL MICROSCOPY ROBERT H. WEBB 3 6 6 11 11 12 14 14 16 16
17 17 18 18 18 2. PRACTICAL CONSIDERATIONS IN ACQUIRING BIOLOGICAL
SIGNALS FROM CONFOCAL MICROSCOPE HAO HE, ROSE CHIK YING ONG, KAM TAI
CHAN, HO PUI HO, AND SIU KAI KONG I. INTRODUCTION 21 II. LASER-INDUCED
RISE OF FLUORESCENCE SIGNALS 22 III. WATER CONTENT OF DMSO AND
EFFICIENCY OF DYE LOADING 23 REFERENCES 26 I. II. III. IV. V. VI. VII.
VIII. IX. X. XI. XII. XIII. XIV. A SIMPLE VIEW OPTICAL SECTIONING
POINT-SPREAD FUNCTION PINHOLE MAGNIFICATION COMPLETE MICROSCOPE
VARIETIES OF CONFOCAL MICROSCOPE MULTIPHOTON MICROSCOPE LIGHT SOURCES
INCOHERENT SOURCES BLEACHING COLOCALITY NUMERICAL APERTURE SUMMARY
REFERENCES CONTENTS 3. EQUIPMENT FOR MASS STORAGE AND PROCESSING OF DATA
GUY COX I. MASS STORAGE 28 II. IMAGE PROCESSING 33 APPENDIX 45
REFERENCES 45 4. ANTIFADING AGENTS FOR CONFOCAL FLUORESCENCE MICROSCOPY
MIGUEL BERRIOS, KIMBERLY A. CONLON, AND DAVID E. COLFLESH I.
INTRODUCTION TO THE REVISED CHAPTER 47 II. INTRODUCTION 48 III. SPECIMEN
PREPARATION 50 IV. ANTIFADING AGENTS 58 V. ACQUISITION OF FADING
FLUORESCENT IMAGES 64 APPENDIX: VENDORS 69 REFERENCES 70 5. MOUNTING
TECHNIQUES FOR CONFOCAL MICROSCOPY MANABU KAGAYAMA AND YASUYUKI SASANO
I. INTRODUCTION 73 II. MATERIALS AND METHODS 74 III. RESULTS AND
DISCUSSIONS 76 REFERENCES 77 6. PREPARATION OF WHOLE MOUNTS AND THICK
SECTIONS FOR CONFOCAL MICROSCOPY HISASHI HASHIMOTO, HIROSHI ISHIKAWA,
AND MORIAKI KUSAKABE I. UPDATE . 79 II. INTRODUCTION 80 III. FIXATION
83 IV. SECTIONING 86 V. PRETREATMENT 87 VI. FLUORESCENT STAINING 90
VII. VASCULAR CAST WITH FLUOROCHROME-LABELED GELATIN 90 VIII. MOUNTING
93 IX. OBSERVATION 97 APPENDIX 99 REFERENCES 103 CONTENTS 7. USE OF
CONFOCAL MICROSCOPY TO INVESTIGATE CELL STRUCTURE AND FUNCTION GHASSAN
BKAILY AND DANIELLE JACQUES I. INTRODUCTION 106 II. PREPARATIONS USED IN
CONFOCAL MICROSCOPY 107 III. SETTINGS OF CONFOCAL MICROSCOPE 108 IV.
ORGANELLE FLUORESCENT PROBES 108 V. VOLUME RENDERING AND NUCLEAR
CALCIUM, SODIUM, AND PH MEASUREMENTS 110 VI. RAPID SCAN IMAGING 110 VII.
COCULTURE OF CELLS 112 V^III. LOADING OF FLUORESCENT PROBES FOR CONFOCAL
MICROSCOPY 113 IX. CONCLUSION 120 REFERENCES 122 COMBINING LASER
SCANNING CONFOCAL MICROSCOPY AND ELECTRON MICROSCOPY XUEJUN SUN I.
UPDATE 124 II. INTRODUCTION 125 III. EXPERIMENTAL PROCEDURES 126 IV.
METHOD 1: IMMUNOGOLD FLUORESCENCE METHOD 127 V. METHOD 2: AVIDIN*BIOTIN
COMPLEX METHOD 133 VI. APPLICATION OF THE METHOD TO IMMUNOCYTOCHEMISTRY
136 VII. DISCUSSION AND CONCLUSION 137 REFERENCES 139 PART II FUNCTIONAL
APPROACHES 9. VOLUME MEASUREMENTS IN CONFOCAL MICROSCOPY CARLOS B.
MANTILLA, Y. S. PRAKASH, AND GARY C. SIECK I. INTRODUCTION * 143 II.
SELECTION OF OPTICS IN CONFOCAL MICROSCOPY ; 145 III. OPTICAL SECTIONING
USING CONFOCAL MICROSCOPY 146 IV. ESTIMATION OF ERRORS IN CONFOCAL
VOLUME MEASUREMENTS 152 V. PRACTICAL LIMITATIONS OF USING CONFOCAL
MICROSCOPY FOR VOLUME MEASUREMENTS 154 REFERENCES 160 10. QUANTITATION
OF PHAGOCYTOSIS BY CONFOCAL MICROSCOPY GEORGE F. BABCOCK AND CHAD T.
ROBINSON I. UPDATE 163 II. QUANTIFICATION OF PHAGOCYTOSIS BY CONFOCAL
MICROSCOPY 165 III. OPSONINS AND RECEPTORS 166 CONTENTS IV. METHODS USED
TO MEASURE PHAGOCYTOSIS 166 V. CHOOSING PROPER PARTICLE FOR PHAGOCYTOSIS
167 VI. FLUOROCHROME LABELING OF BACTERIA 168 VII. EQUIPMENT 168 VIII.
SEPARATION OF EXTERNAL FROM INTERNAL PARTICLES 169 IX. QUANTIFICATION OF
PHAGOCYTOSIS BY CONFOCAL MICROSCOPY 170 X. DATA ANALYSIS AND
INTERPRETATION 172 XI. SUMMARY 173 REFERENCES 173 11. RECEPTOR*LIGAND
INTERNALIZATION GUIDO ORLANDINI, RITA GATTI, GIAN CARLO GAZZOLA,
ALBERICO BORGHETTI, AND NICOLETTA RONDA I. INTRODUCTORY UPDATE 175 II.
INTRODUCTION 177 III. MATERIALS 178 IV. CELL CULTURE 182 V. MEMBRANE
BINDING OF LIGANDS 182 VI. RECEPTOR*LIGAND INTERNALIZATION 183 VII.
TWO-STEP STAINING FOR IDENTIFICATION OF SUBCELLULAR STRUCTURES 186
REFERENCES 187 12. QUANTITATIVE IMAGING OF METABOLISM BY TWO-PHOTON
EXCITATION MICROSCOPY DAVID W. PISTON AND SUSAN M. KNOBEL I.
INTRODUCTION 189 II. USE OF NAD(P)H AUTOFLUORESCENCE FOR METABOLIC
IMAGING 190 III. TWO-PHOTON EXCITATION MICROSCOPY 191 IV. APPLICATIONS
TO QUANTITATIVE METABOLIC IMAGING 195 REFERENCES 204 13. TRAFFICKING OF
THE ANDROGEN RECEPTOR VIRGINIE GEORGET, BEATRICE TEROUANNE, JEAN-CLAUDE
NICOLAS, AND CHARLES SULTAN I. INTRODUCTION 205 II. PLASMID CONSTRUCTION
AND FUNCTIONAL PROPERTIES OF GFP*AR 206 III. GFP-AR LOCALIZATION BY
FLUORESCENCE AND BY IMMUNODETECTION 209 IV. VARIOUS FLUORESCENCE
MICROSCOPE TECHNOLOGIES FOR GFP ANALYSIS 212 V. INTRACEUULAR DYNAMICS
AND QUANTIFICATION OF GFP*AR IN LIVING CELLS 213 VI. APPLICATIONS OF
GFP-AR 215 REFERENCES 219 CONTENTS 14. CONFOCAL MICROSCOPY: KALLIKREIN
PROTEASES AND KININ PEPTIDE RECEPTORS KANTI D. BHOOLA, CELIAJ. SNYMAN,
AND CARLOS D. FIGUEROA I. PREAMBLE 222 II. INTRODUCTION 225 III. METHODS
AND PROCEDURES 238 IV. IMAGES OF TISSUE KALLIKREIN (PROTEASE) AND KININ
RECEPTORS (PEPTIDE RECEPTORS) CAPTURED BY CONFOCAL MICROSCOPY 242 V.
VISUALIZATION OF PROTEASES AND RECEPTORS ON NONFIXED CELLS BY CONFOCAL
MICROSCOPY 243 4VI. VISUALIZATION OF PROTEASES AND RECEPTORS ON FIXED
CELLS BY CONFOCAL MICROSCOPY 245 REFERENCES 258 15. MULTIPHOTON
EXCITATION MICROSCOPY, CONFOCAL MICROSCOPY, AND SPECTROSCOPY OF LIVING
CELLS AND TISSUES; FUNCTIONAL METABOLIC IMAGING OF HUMAN SKIN IN VIVO
BARRY R. MASTERS, PETER T. C. SO, KI HEAN KIM, CHRISTOF BUEHLER, AND
ENRICO GRATTON I. INTRODUCTION 262 II. FUNCTIONAL METABOLIC IMAGING OF
CELLULAR METABOLISM BASED ON NAD(P)H FLUORESCENCE 264 III. ULTRAVIOLET
CONFOCAL FLUORESCENCE MICROSCOPY COMPARED WITH MULTIPHOTON EXCITATION
MICROSCOPY 265 IV. LASER SOURCES FOR MULTIPHOTON EXCITATION MICROSCOPY
268 V. PULSE COMPENSATION TECHNIQUES 270 VI. MULTIPHOTON EXCITATION
MICROSCOPE 271 VII. SIMULTANEOUS MULTIPHOTON EXCITATION MICROSCOPY AND
REFLECTED LIGHT CONFOCAL MICROSCOPY 274 VIII. VIDEO RATE MULTIPHOTON
EXCITATION MICROSCOPY 274 IX. MULTIPHOTON EXCITATION MICROSCOPY OF IN
VIVO HUMAN SKIN: EMISSION SPECTROSCOPY AND FLUORESCENT LIFETIME
MEASUREMENTS . 276 X. USE OF LASER PULSE PICKER TO MITIGATE TWO-PHOTON
EXCITATION- DAMAGE TO LIVING SPECIMENS 280 REFERENCES / 281 16.
VIDEO-RATE, SCANNING SLIT CONFOCAL MICROSCOPY OF LIVING HUMAN CORNEA IN
VIVO: THREE-DIMENSIONAL CONFOCAL MICROSCOPY OF THE EYE BARRY R. MASTERS
AND MATTHIAS BB HNKE I. BIOMICROSCOPY OF THE LIVING EYE FROM SLIT LAMP
TO CONFOCAL MICROSCOPE 283 II. OPTICAL PRINCIPLES OF CONFOCAL MICROSCOPE
284 III. OBJECTIVE SCANNING CONFOCAL MICROSCOPE 285 IV. CONFOCAL
MICROSCOPY OF CORNEA 286 V. OPTICAL LOW-COHERENCE REFLECTOMETRY TO
MEASURE POSITION WITHIN CORNEA 294 CONTENTS VI. CHNICAL EXAMINATION
TECHNIQUE WITH CONFOCAL MICROSCOPE 295 VII. CLINICAL FINDINGS WITH
SCANNING SLIT CONFOCAL MICROSCOPE 300 VIII. THREE-DIMENSIONAL CONFOCAL
MICROSCOPY AND VISUALIZATION OF THE LIVING EYE 303 REFERENCES 305 17. IN
VIVO IMAGING OF MAMMALIAN CENTRAL NERVOUS SYSTEM NEURONS WITH THE IN
VIVO CONFOCAL NEUROIMAGING (ICON) METHOD SYLVIA PRILLOFF, PETRA
HENRICH-NOACK, RALF ENGELMANN, AND BERNHARD A. SABEL I. UPDATE 307 II.
INTRODUCTION 309 III. EQUIPMENT 310 IV. ANIMAL PREPARATION 312 V.
APPLICATIONS AND FUTURE OF ICON 313 VI. SUPPLIERS 315 REFERENCES 315 18.
IDENTIFICATION OF VIRAL INFECTION BY CONFOCAL MICROSCOPY DAVID N. HOWELL
AND SARA E. MILLER I. INTRODUCTION 317 II. CONFOCAL MICROSCOPY 319 III.
ELECTRON MICROSCOPY 329 IV. SUMMARY 331 REFERENCES 332 19. MEMBRANE
TRAFFICKING SABINE KUPZIG, SAN SAN LEE, AND GEORGE BANTING I.
INTRODUCTION * 335 II. METHODS 336 III. FUTURE DEVELOPMENTS 341
REFERENCES 342 PART III GREEN FLUORESCENT PROTEIN 20. MONITORING OF
PROTEIN SECRETION WITH GREEN FLUORESCENT PROTEIN CHRISTOPH KAETHER AND
HANS-HERMANN GERDES I. INTRODUCTION 345 II. CONSTITUTIVE SECRETION 346
III. REGULATED SECRETION 352 REFERENCES 353 CONTENTS 21. COMPARISON OF
ENHANCED GREEN FLUORESCENT PROTEIN AND ITS DESTABILIZED FORM AS
TRANSCRIPTION REPORTERS XIAONING ZHAO, TOMMY DUONG, CHIAO-CHIAN HUANG,
STEVEN R. KAIN, AND XIANQIANG LI I. INTRODUCTION 355 II. PRINCIPLE OF
METHODS 356 III. PROCEDURE 357 IV. CONCLUSION AND REMARKS 359 REFERENCES
361 22. MEASURING PROTEIN DEGRADATION WITH GREEN FLUORESCENT PROTEIN
STEPHEN R. CRONIN AND RANDOLPH Y. HAMPTON I. INTRODUCTION 363 II.
HMG-COA REDUCTASE 365 III. HMG2P-GFP REPORTER PROTEIN 365 IV. DIRECT
VISUAL EXAMINATION OF HMG2P*GFP 367 V. FLOW MICROFLUORIMETRY AND
FLUORESCENCE-ACTIVATED CELL SORTING ANALYSIS OF DEGRADATION 369 VI.
DIRECT EXAMINATION OF HMG2P*GFP IN LIVING COLONIES 372 VII. CAVEATS AND
CONSIDERATIONS 374 REFERENCES 377 23. STUDYING NUCLEAR RECEPTORS WITH
GREEN FLUORESCENT PROTEIN FUSIONS GORDON L. HAGER I. INTRODUCTION 379
II. TAGGING NUCLEAR RECEPTORS WITH GFP 380 III. CHARACTERIZATION OF
FUSION CHIMERA 383 IV. CHOICE OF FLUORESCENT PROTEIN TO BE UTILIZED FOR
RECEPTOR LABELING 384 V. MODE OF EXPRESSION 386 VI. INSTRUMENTATION 386
VII. FUTURE POTENTIAL 387 REFERENCES * 388 / 24. SIGNALING,
DESENSITIZATION, AND TRAFFICKING OF G PROTEIN-COUPLED RECEPTORS REVEALED
BY GREEN FLUORESCENT PROTEIN CONJUGATES LARRY S. BARAK, JIE ZHANG,
STEPHEN S. G. FERGUSON, STEPHANE A. LAPORTE, AND MARC G. CARON I.
INTRODUCTION 391 II. PROPERTIES OF G PROTEIN-COUPLED RECEPTORS, G
PROTEIN-COUPLED RECEPTOR KINASES, AND /?-ARRESTINS 392 III. DESIGN OF
GFP-CONJUGATED G PROTEIN-COUPLED RECEPTORS, G PROTEIN-COUPLED RECEPTOR
KINASES, AND /5-ARRESTINS 395 IV. CHARACTERIZATION OF GFP-CONJUGATED G
PROTEIN-COUPLED RECEPTORS, G PROTEIN-COUPLED RECEPTOR KINASES, AND
/J-ARRESTINS 396 CONTENTS V. INSTRUMENTATION AND METHODOLOGY 398 VI.
CELL DISTRIBUTION OF J?2AR-GFP AND Y326A-02AR-GFP 399 VII. DETERMINATION
OF RECEPTOR ACTIVATION BY GFP-CONJUGATED G PROTEIN-COUPLED RECEPTOR
KINASE AND /?-ARRESTIN 401 VIII. ANALYSIS OF /?-ARRESTIN 2-GFP
TRANSLOCATION THAT OCCURS IN SINGLE CELLS 403 IX. LIMITATIONS 404 X.
CONCLUSION 405 REFERENCES 406 25. FLUORESCENT PROTEINS IN SINGLE- AND
MULTICOLOR FLOW CYTOMETRY LONNIE LYBARGER AND ROBERT CHERVENAK I. UPDATE
409 II. INTRODUCTION 410 III. CHOICE OF FLUORESCENT PROTEINS 411 IV.
TRANSFECTION OF MAMMALIAN CELLS 411 V. STABLE FLUORESCENT PROTEIN
EXPRESSION 413 VI. COMBINING FLUORESCENT PROTEINS WITH COMMON
FLUOROCHROMES 417 VII. CONCLUSIONS 419 REFERENCES 419 26. JELLYFISH
GREEN FLUORESCENT PROTEIN: A TOOL FOR STUDYING ION CHANNELS AND
SECOND-MESSENGER SIGNALING IN NEURONS L. A. C. BLAIR, K. K. BENCE, ANDJ.
MARSHALL I. INTRODUCTION 421 II. PRIMARY CULTURES OF CEREBELLAR GRANULE
NEURONS 422 III. GREEN FLUORESCENT PROTEIN AND GENE-OF-INTEREST
CONSTRUCTS 425 IV. TRANSIENT TRANSFECTION OF NEURONS BY CALCIUM
PHOSPHATE PRECIPITATION 428 V. LIPOFECTAMINE TRANSFECTION IN CELL LINES
429 VI. APPLICATIONS TO BIOLOGICAL PROBLEMS 429 REFERENCES 432 27.
EXPRESSION OF GREEN FLUORESCENT PROTEIN AND INOSITOL 1,4,5-TRIPHOSPHATE
RECEPTOR IN XENOPUS LAEVIS OOCYTES ATSUSHI MIYAWAKI, JULIE M. MATHESON,
LEE G. SAYERS, AKIRA MUTO, TAKAYUKI MICHIKAWA, TEIICHI FURUICHI, AND
KATSUHIKO MIKOSHIBA I. INTRODUCTION 433 II. PROCEDURES 434 III. USE OF
GFP FOR EVALUATION OF RELATIVE EXPRESSION LEVELS OF EXOGENOUS PROTEIN IN
XENOPUS OOCYTES 437 IV. GFP LOCALIZATION OF INTRACEUULAR IPJR-CA STORES
IN XENOPUS OOCYTES 438 REFERENCES 442 CONTENTS 28. CONFOCAL IMAGING OF
CA 2+ , PH, ELECTRICAL POTENTIAL, AND MEMBRANE PERMEABILITY IN SINGLE
LIVING CELLS JOHNJ. LEMASTERS, DONNA R. TROLLINGER, TING QIAN, WAYNE E.
CASCIO, AND HISAYUKI OHATA I. INTRODUCTION 444 II. FORMATION OF OPTICAL
SECTIONS BY CONFOCAL MICROSCOPY 444 III. PRECAUTIONS AGAINST PHOTODAMAGE
AND PHOTOBLEACHING 446 IV. PREPARATION OF CEUS FOR MICROSCOPY 447 V.
MULTICOLOR FLUORESCENCE 448 VI. IMAGING ELECTRICAL POTENTIAL WITH
CATIONIC FLUOROPHORES 448 VII. PRINCIPLE OF RATIO IMAGING 449 VIII.
COMPARTMENTATION OF ESTER-LOADED FLUOROPHORES 451 IX. MEMBRANE
PERMEABILITY 453 X. CONFOCAL IMAGING OF CA + 454 XI. CONCLUSION 459
REFERENCES 460 PART IV LASER CAPTURE MICRODISSECTION 29. LASER CAPTURE
MICRODISSECTION AND ITS APPLICATIONS IN GENOMICS AND PROTEOMICS JAMES L.
WITTLIFF I. BACKGROUND 463 II. PROTOCOL FOR PROCESSING HUMAN TISSUE
SPECIMENS 465 III. ADDITIONAL APPLICATIONS OF LASER CAPTURE
MICRODISSECTION 475 REFERENCES 476 30. GOING IN VIVO WITH LASER
MICRODISSECTION ANETTE MAYER, MONIKA STICH, DIETER BROCKSCH, KARIN
SCHUTZE, AND GEORGIA LAHR I. INTRODUCTION 479 II. STEP I: CEU CULTURE
PREPARATION / 480 III. STEP II: COLLECTION OF CATAPULTED CEUS 485 IV.
STEP III: RECULTIVATION OF CATAPULTED CEUS 486 V. FUTURE APPLICATIONS
486 REFERENCES 487 CONTENTS 31. FLUORESCENCE IN SITU HYBRIDIZATION OF
LCM-ISOLATED NUCLEI FROM PARAFFIN SECTIONS DOUGLAS J. DEMETRICK, LISA M.
DIFRANCESCO, AND SABITA K. MURTHY I. INTRODUCTION 489 II. MATERIALS AND
METHODS 491 III. DISCUSSION 492 IV. TROUBLESHOOTING TIPS 494 REFERENCES
496 INDEX 499
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| spelling | Techniques in confocal microscopy ed. by P. Michael Conn London [u.a.] Acad. Press 2010 XXI, 511 S., [9] Bl. Ill. txt rdacontent n rdamedia nc rdacarrier Reliable lab solutions Konfokale Mikroskopie (DE-588)4336446-9 gnd rswk-swf Konfokale Mikroskopie (DE-588)4336446-9 s DE-604 Conn, Patrick Michael 1949-2016 Sonstige (DE-588)136758541 oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020641815&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Techniques in confocal microscopy Konfokale Mikroskopie (DE-588)4336446-9 gnd |
| subject_GND | (DE-588)4336446-9 |
| title | Techniques in confocal microscopy |
| title_auth | Techniques in confocal microscopy |
| title_exact_search | Techniques in confocal microscopy |
| title_full | Techniques in confocal microscopy ed. by P. Michael Conn |
| title_fullStr | Techniques in confocal microscopy ed. by P. Michael Conn |
| title_full_unstemmed | Techniques in confocal microscopy ed. by P. Michael Conn |
| title_short | Techniques in confocal microscopy |
| title_sort | techniques in confocal microscopy |
| topic | Konfokale Mikroskopie (DE-588)4336446-9 gnd |
| topic_facet | Konfokale Mikroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020641815&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT connpatrickmichael techniquesinconfocalmicroscopy |