Thiol redox transitions in cell signaling: B Cellular localization and signaling
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Amsterdam [u.a.]
Elsevier, AP
2010
|
| Edition: | 1. ed. |
| Series: | Methods in enzymology
474 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XLVIII, 340 S. Ill., graph. Darst. |
| ISBN: | 9780123810038 |
Staff View
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Record in the Search Index
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|---|---|
| adam_text | Contents
Contributors
xiii
Preface
xix
Volumes in Series
xxi
1.
Engineering of Fluorescent Reporters into
Redox
Domains
to Monitor Electron Transfers
1
Derek Parsonage, Stacy A. Reeves, P. Andrew Karplus, and Leslie B. Poole
1.
Introduction
2
2.
The Problem: Low Sensitivity and Improperly Rate-Limited
Assays for
Redox
Functions of Bacterial Peroxiredoxin Systems
3
3.
The Solution: Engineering of Fluorescent
Redox
Reporters
into the
N-Terminal
Domain of AhpF and
£
coli Grxl
4
4.
Engineering of Disulfide-Containing Electron Acceptor Domains
to Detect Electron Transfers via Fluorescence Changes; Linkage
of Fluorescein to Bacterial AhpC via a Reducible Disulfide Bond
7
5.
Materials
8
6.
Methods
10
7.
Summary
19
References
19
2.
Blot-Based Detection of Dehydroalanine-Containing Glutathione
Peroxidase with the Use of Biotin-Conjugated Cysteamine
23
Sue Goo Rhee and Chun-Seok Cho
1.
Introduction
24
2.
Oxidative Inactivation of Glutathione Peroxidase and the
Conversion of Its Active Site Sec to
D
HA
25
3.
Preparation of Biotin-Conjugated Cysteamine
27
4.
Blot-Based Detection of DHA-GPxl in RBCs
28
5.
Effects of Oxidative Stress on the Formation of DHA-GPxl
in RBCs
29
6.
Concluding Remarks
30
Acknowledgment
32
References
32
v¡
Contents
3.
Analysis of the
Redox
Regulation of Protein Tyrosine
Phosphatase Superfamily Members Utilizing a
Cysteinyl-Labeling Assay
35
Benoit
Boivin and Nicholas K. Tonks
1.
Introduction
36
2.
Active-Site Structure, Catalysis, and Oxidation
37
3.
Detection Methods
39
4.
General Principle of the Assay
40
5.
Solutions
42
6.
Preparation of the Lysis Buffer
42
7.
Preparation of the
Hypoxie
Glove Box
43
8.
Preparation of Cell Lysates
43
9.
Cysteinyl-Labeling Assay
44
10.
Acute Stimulus-Induced Reversible Oxidation of PTPs
44
11.
Perspectives
45
12.
Conclusion
48
References
48
4.
Measuring the
Redox
State of Cellular Peroxiredoxins
by Immunoblotting
51
Andrew G. Cox, Christine
С
Winterboum, and Mark B. Hampton
1.
Introduction
52
2.
Measurement of Prx Dimerization
54
3.
Measurement of Prx Hyperoxidation
59
4.
Discussion
61
Acknowledgments
64
References
64
5.
Thiol
Redox
Transitions by Thioredoxin
and Thioredoxin-Binding Protein-2 in Cell Signaling
67
Eiji Yoshihara, Zhe Chen, Yoshiyuki Matsuo, Hiroshi Masutani,
and Junji Yodoi
1.
Functional Regulation of
Redox-Sensitive
Proteins by
Thiol Modification
68
2.
Thiol Reduction by the Thioredoxin
Redox
System
70
3.
Thioredoxin Superfamily
73
4.
Reversible
Redox
and Signal Regulation by Thioredoxin and
Thioredoxin-binding Protein-2 (TBP-2)
74
5.
Conclusion
76
References
76
Contents
vii
6.
Detection of Protein Thiols in Mitochondrial Oxidative
Phosphorylation Complexes and Associated Proteins
83
Kelly
K. Andringa
and Shannon M. Bailey
1.
Introduction
84
2.
Mitochondria Isolation and Protein Thiol Labeling
86
3.
Application of Blue Native-PAGE for the Isolation of Oxidative
Phosphorylation Protein Subunits and Other Proteins Associated
with the Complexes
89
4.
Detection of IBTP-Labeled Protein Thiols in Protein Complexes
94
5.
Analysis and Mass Spectrometry Identification of Protein
95
6.
Other Considerations
104
7.
Conclusion
105
Acknowledgments
106
References
106
7.
Mitochondrial Thioredoxin
Reducíase:
Purification,
Inhibitor Studies, and Role in Cell Signaling
109
Maria
Pia Rigobello
and Alberto
Bindoli
1.
Introduction
110
2.
Purification of Thioredoxin
Reducíase
from Isolated
Mitochondria, Cultured Cells, and Whole Organs 111
3.
Estimation of Thioredoxin
Reducíase
Activity
117
4.
Inhibitor Sludies of Thioredoxin
Reducíase
118
5.
Role in Cell Signaling
118
References
120
8.
Measuring Mitochondrial Protein Thiol
Redox
State
123
Raquel
Requejo, Edward T. Chouchani, Thomas R.
Hurd,
Kalja
E. Menger, Mark
В.
Hampion,
and Michael P. Murphy
1.
Introduction
124
2.
Quantification of Mitochondrial Protein Thiols
127
3.
Quantification of Glutathionylation of Mitochondrial Proteins
130
4.
Assessment of S-Nitrosated Protein Thiols
135
5.
Measurement of the Thioredoxin and Peroxiredoxin
Redox
States
139
6.
Conclusions
143
Acknowledgments
144
References
144
viii Contents
9.
Measurement of Extracellular (Exofacial) Versus Intracellular
Protein Thiols
149
Jolanta
Skalska,
Steven Bernstein, and Paul Brookes
1.
Measurement of Mitochondrial Thiol Status
150
2.
Measurement of Cytosolic Thiol Status
156
3.
Measurement of Exofacial Thiols
157
4.
Exofacial Thiol Status and Cancer
160
References
162
10.
Redox
Clamp Model for Study of Extracellular Thiols
and Disulfides in
Redox
Signaling
165
Young-Mi Go and Dean P. Jones
1.
Introduction
166
2.
Key Concepts for Use
166
3.
Principles for Experimental Design
167
4.
Summary of Available
Redox
Clamp Studies
171
5.
Perspectives and Conclusion
177
Acknowledgments
178
References
178
11.
Redox
State of Human Serum Albumin in Terms
of Cysteine-34 in Health and Disease
181
Karl Oettl and
Günther Marsche
1.
Background
182
2.
HPLC Analysis
182
3.
Albumin Thiol State and Exercise
187
4.
Influence of Supplementation
188
5.
Albumin Oxidation in Disease
188
6.
Albumin Thiol State During Aging
191
7.
Summary
193
References
193
12.
Methods for Studying
Redox
Cycling of Thioredoxin
in Mediating Preconditioning-lnduced Survival Genes
and Proteins
197
Chuang
С
Chiueh
1.
Hormetic Mechanism: Role of
Redox
Cycling of Thioredoxin
198
2.
Redox
Functioning of Thioredoxin
199
Contents
¡χ
3.
Implications
of Preconditioning Protection
from Preclinical and Clinical Studies
200
4.
Drugs Mimic Thioredoxin-Medicated Preconditioning-lnduced
Signaling and Protection in Cells
202
5.
Methods and Materials
203
References
209
13.
Oxidative Stress, Thiol
Redox
Signaling Methods
in Epigenetics
213
Isaac K. Sundar, Samuel
Caito, Hongwei
Yao, and Irfan Rahman
1.
Introduction
216
2.
Histone Acetylation Assays Using [3H]-Acetate
Incorporation
219
3.
Histone Acetylation by Immunoblotting
221
4.
HAT Activity Assay
222
5.
HDAC Activity Assay Using [3H]-Labeled Histones
223
6.
HDAC Activity Assay
225
7.
HDACs Levels by Immunoblotting
227
8.
Posttranslational Modifications of HDACs and SIRTs
(Sirtuins
1-7)
by Immunoprecipitation
228
9.
Preparation of Whole Cell
Lysáte
229
10.
Preparation of Cytoplasmic and Nuclear Proteins
229
11.
Redox-Mediated Posttranslational Modification Assays
230
12.
Chromatin Immunoprecipitation (ChIP) Assay
233
13.
Conclusions
238
Acknowledgments
239
References
239
14.
Characterization of Protein Targets of Mammalian
Thioredoxin Reductases
245
Anton A. Turanov, Dolph L. Hatfield, and
Vadim
N.
Gtadyshev
1.
Introduction
246
2.
Preparation of TR-immobilized Affinity Resins
247
3.
Identification of Targets of Mammalian
TRs in Cell Lysates
250
4.
Concluding Remarks and Future Perspectives
252
Acknowledgments
253
References
253
Contents
15.
Alteration of Thioredoxin
Reducíase
1
Levels in
Elucidating Cancer Etiology
255
Min-Hyuk Yoo, Bradley A. Carlson,
Petra Tsuji,
Robert Irons,
Vadim
N.
Gladyshev, and Dolph
L Hatfield
1.
Introduction
256
2.
Materials and Methods
257
3.
Results and Discussion
263
4.
Conclusions and Future Perspectives
271
Acknowledgments
272
References
272
16.
Regulation of Apoptosis Signal-Regulating Kinase
1
in
Redox
Signaling
277
Kazumi Katagiri, Atsushi Matsuzawa, and Hidenori Ichijo
1.
Overview
278
2.
Materials
281
3.
Methods
283
4.
Comment
286
References
287
17.
Protocols for the Detection of S-Glutathionylated
and S-Nitrosylated Proteins In Situ
289
Scott W. Aesif, Yvonne M. W. Janssen-Heininger,
and
Niki
L.
Reynaert
1.
Introduction
290
2.
Protein S-Glutathionylation
290
3.
Protein S-Nitrosylation
293
4.
Summary
295
References
295
18.
Synthesis, Quantification, Characterization, and
Signaling Properties of Gtutathionyl Conjugates of Enals
297
Sanjay Srivastava,
Kota
V.
Ramana,
Aruni Bhatnagar,
and Satish K. Srivastava
1.
Introduction
298
2.
Synthesis, Quantification, and Characterization of Reagent
Glutathionyl Conjugates of
HNE
300
3.
Metabolism of
HNE 305
Contents xi
4.
Signaling Properties of Gtutathionyl Conjugates of
HNE
309
5.
Conclusions
310
Acknowledgments
311
References
311
19.
Thioredoxin and
Redox
Signaling in Vasculature—Studies
Using Trx2 Endothelium-Specific Transgenic Mice
315
Wang
Min, Luyao
(Kevin) Xu, Huanjiao (jenny) Zhou,
Qunhua Huang, Haifeng Zhang, Yun He, Xu Zhe, and Yan Luo
1.
Introduction
316
2.
Methods
318
References
323
Author Index
325
Subject Index
333
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| genre_facet | Aufsatzsammlung |
| id | DE-604.BV036667122 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:45:18Z |
| institution | BVB |
| isbn | 9780123810038 |
| language | English |
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| publishDateSearch | 2010 |
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| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Thiol redox transitions in cell signaling B Cellular localization and signaling ed. by Enrique Cadenas ... 1. ed. Amsterdam [u.a.] Elsevier, AP 2010 XLVIII, 340 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 474 Methods in enzymology ... Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Thiole (DE-588)4185267-9 gnd rswk-swf Redoxsystem (DE-588)4177304-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Thiole (DE-588)4185267-9 s Redoxsystem (DE-588)4177304-4 s Signaltransduktion (DE-588)4318717-1 s DE-604 Cadenas, Enrique Sonstige oth (DE-604)BV036555262 2 Methods in enzymology 474 (DE-604)BV000000938 474 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020586299&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Thiol redox transitions in cell signaling Methods in enzymology Signaltransduktion (DE-588)4318717-1 gnd Thiole (DE-588)4185267-9 gnd Redoxsystem (DE-588)4177304-4 gnd |
| subject_GND | (DE-588)4318717-1 (DE-588)4185267-9 (DE-588)4177304-4 (DE-588)4143413-4 |
| title | Thiol redox transitions in cell signaling |
| title_auth | Thiol redox transitions in cell signaling |
| title_exact_search | Thiol redox transitions in cell signaling |
| title_full | Thiol redox transitions in cell signaling B Cellular localization and signaling ed. by Enrique Cadenas ... |
| title_fullStr | Thiol redox transitions in cell signaling B Cellular localization and signaling ed. by Enrique Cadenas ... |
| title_full_unstemmed | Thiol redox transitions in cell signaling B Cellular localization and signaling ed. by Enrique Cadenas ... |
| title_short | Thiol redox transitions in cell signaling |
| title_sort | thiol redox transitions in cell signaling cellular localization and signaling |
| topic | Signaltransduktion (DE-588)4318717-1 gnd Thiole (DE-588)4185267-9 gnd Redoxsystem (DE-588)4177304-4 gnd |
| topic_facet | Signaltransduktion Thiole Redoxsystem Aufsatzsammlung |
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