Analysis of hematopoietic plasticity and B cell development in progenitors with regulable hPax5 expression:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Berlin
mbv, Mensch-und-Buch-Verl.
2010
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| Series: | Biological science
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | VIII, 99 S. graph. Darst. |
| ISBN: | 9783866647909 |
Staff View
MARC
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| 245 | 1 | 0 | |a Analysis of hematopoietic plasticity and B cell development in progenitors with regulable hPax5 expression |c vorgelegt von Szandor Simmons |
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Record in the Search Index
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| adam_text | 1. TABLE OF CONTENTS 1. TABLE OF CONTENTS I 2. ABSTRACT V 3.
ZUSAMMENFASSUNG VII 4. INTRODUCTION 1 4.1 HEMATOPOIETIC COMMITMENT *
FROM PLURIPOTENT HEMATOPOIETIC STEM CELLS TO MATURE BLOOD CELLS AND BACK
AGAIN 1 4.1.1 THE HIERARCHIC MODEL OF HEMATOPOIESIS 1 4.1.2 THE
COMPETENT SITES OF HEMATOPOIETIC DEVELOPMENT - CROSSTALK BETWEEN THE
MICROENVIRONMENT AND DIFFERENTIATING HEMATOPOIETIC CELLS 1 4.1.3
EPIGENETIC ACCESSIBILITY - A MATTER OF CHROMATIN MODIFICATIONS 5 4.1.4
INSIGHTS INTO PROB, PREB AND B CELL RECEPTOR FORMATION AND FUNCTION 5
4.1.5 THE FLEXIBILITY OF THE HEMATOPOIETIC SYSTEM 7 5. MATERIALS &
METHODS 11 5.1 MICE 11 5.2 MATERIALS 11 5.2.1 MACHINES 11 5.2.2
DISPOSABLES 12 5.2.3 KITS 13 5.2.4 CHEMICALS 13 GENERAL CHEMICALS 13
FINE CHEMICALS 14 BUFFERS & SOLUTIONS 14 5.2.5 BIOLOGICALS 14
MICROBIOLOGY 14 CELL CULTURE 15 DNA, PLASMIDS & RETROVIRAL VECTORS 15
ENZYMES 15 PRIMERS 15 ANTIBODIES 16 AVIDIN CONJUGATES 18 SOFTWARE 18 5.3
METHODS 19 5.3.1 ANIMAL WORK 19 GENERAL INFORMATION 19 CROSSING &
BREEDING 19 GENOTYPING BY PCR 19 BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/1006306218 DIGITALISIERT DURCH 1. TABLE OF CONTENTS
ISOLATION OF ORGANS 21 TAIL CLIPPING AND EAR MARKINGS 21 FEEDING OF
ANTIBIOTICS 22 I.V. AND I.P. TRANSPLANTATION OF TISSUE CULTURE CELLS &
BONE MARROW CELLS 22 5.3.2 CELLULAR WORK 22 MEDIA 22 CELL LINES 23 CELL
CULTURE OF STROMAL CELL LINES AND THE PHOENIX*-ECO VIRUS PRODUCER CELL
LINE 25 CELL CULTURE OF STROMAL CELL/IL-7 DEPENDENT PREB CELL LINES 25
CELL CULTURE OF ANTIBODY OR GROWTH FACTOR PRODUCING HYBRIDOMA CELL LINES
25 ESTABLISHMENT OF PREB CELL LINES AND CLONES FROM DIFFERENT ORGANS OF
MICE.... 26 FREEZING AND THAWING OF TISSUE CULTURE CELLS 26 TRANSDUCTION
OF PHOENIX*-ECO VIRUS PRODUCER CELL LINE 27 TRANSDUCTION OF PREB CELL
LINES WITH PHOENIX*-ECO CELL DERIVED RETROVIRUSES 27 DIFFERENTIATION OF
PAX5-DEFICIENT PREB CELL LINES INTO IGM IMMATURE B CELLS AFTER
CONDITIONAL EXPRESSION OF HPAX5 IN VITRO 28 DIFFERENTIATION OF
PAX5-DEFICIENT PREB CELL LINES INTO BIPHENOTYPIC CD197B2207CD1 LB T
PROGENITOR CELLS IN VITRO 28 TISSUE SAMPLE PREPARATION 28 FACS STAININGS
29 FACS ANALYSIS 29 FACS SORTING 29 CA 2 ~ SIGNALING MEASUREMENTS 29
LIMITING DILUTION ANALYSIS 30 5.3.3 MICROBIOLOGICAL METHODS 31 MEDIA 31
GROWTH AND STORAGE OF BACTERIA 31 PREPARATION OF ELECTRO-COMPETENT
BACTERIA FOR TRANSFORMATION 31 ELECTRO-TRANSFORMATIO 1. TABLE OF COMENLS
6. RESULTS 3 6.1 GENERATION OF HOMOZYGOUS PAX5 AND PAX5/SURROGATE LIGHT
CHAIN KNOCKOUT MICE 37 6.1.1 PAX5~ ~ KNOCKOUT MICE 37 6.1.2
PAX5 SLC KNOCKOUT MICE 37 6.2 GENERATION OF CONDITIONAL RETROVIRAL
HPAX5 EXPRESSION SYSTEMS 40 6.2.1 CLONING OF A TAMOXIFEN (4-OHT)
INDUCIBLE RETROVIRAL HPAXS EXPRESSION VECTOR 40 6.2.2 CLONING OF A
DOXYCYCLINE DEPENDENT RETROVIRAL HPAX5 EXPRESSION VECTOR SYSTEM 41 6.3
CHOOSING THE WEAPONS: TAMOXIFEN INDUCIBLE HPAX5 EXPRESSION VECTOR VS.
DOXYCYCLINE DEPENDENT HPAX5 EXPRESSION SYSTEM 42 6.4 CHARACTERIZATION OF
PAX5 SLC^ ~ AND PAX5 SLC PRO/PREB-I CELL LINES 45 6.4.1 LOSS OF
SURFACE MARKER EXPRESSION OF PLURIPOTENT PROGENITOR CELLS AND B-LINEAGE
RESTRICTION ON PAX5 SLC* ~ AND PAX5 SLC PRO/PREB-I CELLS AFTER
CONDITIONAL EXPRESSION OF HPAX5 45 6.5 RESCUE OF THE B CELL
DEVELOPMENTAL POTENCY OF PAX5~ SLC~ ~ AND PAX5 SLC PRO/PREB-I CELL
LINES 48 6.5.1 DIFFERENTIATION OF PAX5 SLC* ~ AND PAX5 A SLC
PRO/PREB-I CELL LINES INTO IMMATURE B CELLS IN VITRO 48 6.5.2
DIFFERENTIATION OF PAXS^SLC AND PAX5 SLC PRO/PREB-I CELL LINES
INTO IMMATURE B CELLS IN VIVO 50 6.5.3 DIFFERENTIATION OF PAX5/SLC
DEFICIENT PRO/PREB-I CELLS TRANSDUCED WITH RETROVIRAL EXPRESSION VECTORS
FOR ?.5 AND VPREB 1 IN WILDTYPE OR MUTANT FORM INTO IMMATURE B CELLS IN
1. TABLE OF CONTENTS 6.6.5 A CD197B2207CD1 LB + CELL POPULATION IN
WILDTYPE C57BL6 MICE ACCUMULATE OVER TIME IN VIVO 69 6.7 IL-18R: A NEW
SURFACE MARKER FOR THE DETECTION OF A PROGENITOR CELL POPULATION WITH NK
CELL DEVELOPMENTAL POTENTIAL 71 7. DISCUSSION 76 8. ABBREVIATIONS 86 9.
ACKNOWLEDGEMENTS 89 10. BIBLIOGRAPHY 91
|
| any_adam_object | 1 |
| author | Simmons, Szandor |
| author_facet | Simmons, Szandor |
| author_role | aut |
| author_sort | Simmons, Szandor |
| author_variant | s s ss |
| building | Verbundindex |
| bvnumber | BV036545402 |
| ctrlnum | (OCoLC)669171865 (DE-599)BVBBV036545402 |
| dewey-full | 571.9673835 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.9673835 |
| dewey-search | 571.9673835 |
| dewey-sort | 3571.9673835 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| id | DE-604.BV036545402 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:42:31Z |
| institution | BVB |
| isbn | 9783866647909 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020467100 |
| oclc_num | 669171865 |
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| owner_facet | DE-83 |
| physical | VIII, 99 S. graph. Darst. |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | mbv, Mensch-und-Buch-Verl. |
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| series2 | Biological science |
| spelling | Simmons, Szandor Verfasser aut Analysis of hematopoietic plasticity and B cell development in progenitors with regulable hPax5 expression vorgelegt von Szandor Simmons Berlin mbv, Mensch-und-Buch-Verl. 2010 VIII, 99 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biological science Zugl.: Berlin, Techn. Univ., Diss., 2010 Vorläuferzelle (DE-588)4418410-4 gnd rswk-swf Zelldifferenzierung (DE-588)4067536-1 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf B-Lymphozyt (DE-588)4137121-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content B-Lymphozyt (DE-588)4137121-5 s Vorläuferzelle (DE-588)4418410-4 s Zelldifferenzierung (DE-588)4067536-1 s Genregulation (DE-588)4122166-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020467100&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Simmons, Szandor Analysis of hematopoietic plasticity and B cell development in progenitors with regulable hPax5 expression Vorläuferzelle (DE-588)4418410-4 gnd Zelldifferenzierung (DE-588)4067536-1 gnd Genregulation (DE-588)4122166-7 gnd B-Lymphozyt (DE-588)4137121-5 gnd |
| subject_GND | (DE-588)4418410-4 (DE-588)4067536-1 (DE-588)4122166-7 (DE-588)4137121-5 (DE-588)4113937-9 |
| title | Analysis of hematopoietic plasticity and B cell development in progenitors with regulable hPax5 expression |
| title_auth | Analysis of hematopoietic plasticity and B cell development in progenitors with regulable hPax5 expression |
| title_exact_search | Analysis of hematopoietic plasticity and B cell development in progenitors with regulable hPax5 expression |
| title_full | Analysis of hematopoietic plasticity and B cell development in progenitors with regulable hPax5 expression vorgelegt von Szandor Simmons |
| title_fullStr | Analysis of hematopoietic plasticity and B cell development in progenitors with regulable hPax5 expression vorgelegt von Szandor Simmons |
| title_full_unstemmed | Analysis of hematopoietic plasticity and B cell development in progenitors with regulable hPax5 expression vorgelegt von Szandor Simmons |
| title_short | Analysis of hematopoietic plasticity and B cell development in progenitors with regulable hPax5 expression |
| title_sort | analysis of hematopoietic plasticity and b cell development in progenitors with regulable hpax5 expression |
| topic | Vorläuferzelle (DE-588)4418410-4 gnd Zelldifferenzierung (DE-588)4067536-1 gnd Genregulation (DE-588)4122166-7 gnd B-Lymphozyt (DE-588)4137121-5 gnd |
| topic_facet | Vorläuferzelle Zelldifferenzierung Genregulation B-Lymphozyt Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020467100&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT simmonsszandor analysisofhematopoieticplasticityandbcelldevelopmentinprogenitorswithregulablehpax5expression |