Neutrophil extracellular trap formation in mice:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 95 Bl. Ill., graph. Darst. |
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Record in the Search Index
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| adam_text | TABLE OF CONTENTS TABLE OF CONTENTS ZUSAMMENFASSUNG 1 SUMMARY 2
KEYWORDS/SCHLAGWORTE 3 1. INTRODUCTION 4 1.1. GRANULOPOIESIS 4 1.2.
EXTRAVASATION AND RECRUITMENT TO THE SITE OF INFECTION 6 1.3.
INTRACELLULAR KILLING MECHANISM 7 1.4. EXTRACELLULAR KILLING MECHANISM 9
1.5. NEUTROPHIL EXTRACELLULAR TRAPS 9 1.6. CANDIDA ALBICANS 12 1.7. AIMS
OF THE STUDY 15 2. MATERIAL AND METHODS 16 2.1. CELL BIOLOGICAL METHODS
16 2.1.1. ISOLATION OF BONE MARROW DERIVED NEUTROPHILS FROM MICE 16
2.1.2. ISOLATION OF BLOOD NEUTROPHILS FROM MICE 18 2.1.3. ISOLATION OF
HUMAN PERIPHERAL BLOOD NEUTROPHILS 19 2.1.4. CELL DEATH ASSAY 20 2.1.5.
ROS MEASUREMENT 20 2.2. MICROSCOPICAL METHODS 21 2.2.1.
IMMUNOFLUORESCENCE MICROSCOPY 21 2.2.2. LIVE CELL MICROSCOPY 22 2.2.3.
TRANSMISSION ELECTRON MICROSCOPY 22 2.2.4. SCANNING ELECTRON MICROSCOPY
22 2.3. MICROBIOLOGICAL METHODS 23 2.3.1. C. ALBICANS STRAINS CULTURE 23
2.3.2. BACTERIAL CULTURE 23 2.3.3. KILLINGASSAY 24 BIBLIOGRAFISCHE
INFORMATIONEN HTTP://D-NB.INFO/1002923476 DIGITALISIERT DURCH TABLE OF
CONTENTS 2.4. ANIMAL EXPERIMENTAL METHODS 25 2.4.1. MOUSE BREEDING 25
2.5. STATISTICAL ANALYSIS 25 3. RESULTS 26 3.1. MOUSE NEUTROPHILS
RELEASE NEUTROPHIL EXTRACELLULAR TRAPS IN VITRO 26 3.1.1. ROS
MEASUREMENT 26 3.1.2. CELL DEATH ASSAY 27 3.1.3. SERUM PROLONGS SURVIVAL
OF NEUTROPHILS 28 3.1.4. NET VISUALIZATION 29 3.1.5. SERUM ONASES
DEGRADE NETS 31 3.1.6. NEUTROPHILS UNDERGO DRASTIC MORPHOLOGICAL CHANGES
DURING NET FORMATION 33 3.1.7. PURIFIED MOUSE NEUTROPHILS UNDERGO CELL
DEATH SIMILARTO HUMAN NEUTROPHILS 39 3.2. ROS PRODUCTION CORRELATES WITH
CELL DEATH 41 3.3. ACTIVATION OF NEUTROPHILS WITH PHYSIOLOGICAL STIMULI
IN VITRO 43 3.3.1. BACTERIA ARE NOT SUFFICIENT TO TRIGGER NET RELEASE 43
3.3.2. C. ALBICANS TRIGGERS NET FORMATION 45 3.4. C. ALBICANS CAN ESCAPE
FROM MOUSE NEUTROPHILS 47 3.4.1. C. ALBICANS GROW WITHIN MOUSE
NEUTROPHILS 47 3.4.2. C. ALBICANS WILD-TYPE FORMS HYPHAE IN MOUSE
NEUTROPHILS 51 3.4.3. MOUSE NEUTROPHILS KILL C. ALBICANS LESS EFFICIENT
THAN HUMAN NEUTROPHILS 52 3.4.4. CELL DEATH DUE TO ESCAPING C ALBICANS
IS DIFFERENT FROM NETOSIS 54 4. DISCUSSION 55 4.1. MOUSE NEUTROPHILS
MAKE NETS IN VITRO 55 4.1.1 TABLE OF CONTENTS 4.2. ROS PRODUCTION
CORRELATES WITH CELL DEATH 61 4.3. PHYSIOLOGICAL STIMULI CAN TRIGGER NET
RELEASE IN VITRO 63 4.3.1. BACTERIA DO NOT EFFICIENTLY INDUCE NET
RELEASE 63 4.3.2. C. ALBICANS HYPHAE TRIGGER NETS 64 4.4. C. ALBICANS
ESCAPES FROM MOUSE NEUTROPHILS 65 4.4.1. C. ALBICANS GROWS WITHIN MOUSE
NEUTROPHILS 65 4.4.2. C. O/B/CONS-INDUCED CELL DEATH IS DIFFERENT FROM
NETOSIS 67 4.4.3. HYPHAL GROWTH IS NECESSARY FOR ESCAPING FROM
NEUTROPHILS 68 4.4.4. MOUSE NEUTROPHILS ARE LESS EFFICIENT AT KILLING C.
ALBICANS THAN HUMAN NEUTROPHILS.... 71 5. REFERENCES 73 ABBREVIATIONS 85
PUBLICATIONS 87 ACKNOWLEDGMENTS 88 SELBSTAENDIGKEITSERKLAERUNG 89
BILDNACHWEIS 89
|
| any_adam_object | 1 |
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| author_facet | Ermert, David |
| author_role | aut |
| author_sort | Ermert, David |
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| ctrlnum | (OCoLC)917578479 (DE-599)BVBBV036506949 |
| discipline | Biologie |
| format | Thesis Book |
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| illustrated | Illustrated |
| indexdate | 2024-07-09T22:41:51Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020429245 |
| oclc_num | 917578479 |
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| physical | 95 Bl. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
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| spelling | Ermert, David Verfasser aut Neutrophil extracellular trap formation in mice von David Ermert 2009 95 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Berlin, Humboldt-Univ., Diss., 2009 Neutrophiler Granulozyt (DE-588)4171673-5 gnd rswk-swf Antimikrobieller Wirkstoff (DE-588)4142688-5 gnd rswk-swf Immunreaktion (DE-588)4133841-8 gnd rswk-swf Sekretion (DE-588)4124179-4 gnd rswk-swf Candida albicans (DE-588)4147244-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Neutrophiler Granulozyt (DE-588)4171673-5 s Antimikrobieller Wirkstoff (DE-588)4142688-5 s Sekretion (DE-588)4124179-4 s Immunreaktion (DE-588)4133841-8 s Candida albicans (DE-588)4147244-5 s DE-101 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020429245&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ermert, David Neutrophil extracellular trap formation in mice Neutrophiler Granulozyt (DE-588)4171673-5 gnd Antimikrobieller Wirkstoff (DE-588)4142688-5 gnd Immunreaktion (DE-588)4133841-8 gnd Sekretion (DE-588)4124179-4 gnd Candida albicans (DE-588)4147244-5 gnd |
| subject_GND | (DE-588)4171673-5 (DE-588)4142688-5 (DE-588)4133841-8 (DE-588)4124179-4 (DE-588)4147244-5 (DE-588)4113937-9 |
| title | Neutrophil extracellular trap formation in mice |
| title_auth | Neutrophil extracellular trap formation in mice |
| title_exact_search | Neutrophil extracellular trap formation in mice |
| title_full | Neutrophil extracellular trap formation in mice von David Ermert |
| title_fullStr | Neutrophil extracellular trap formation in mice von David Ermert |
| title_full_unstemmed | Neutrophil extracellular trap formation in mice von David Ermert |
| title_short | Neutrophil extracellular trap formation in mice |
| title_sort | neutrophil extracellular trap formation in mice |
| topic | Neutrophiler Granulozyt (DE-588)4171673-5 gnd Antimikrobieller Wirkstoff (DE-588)4142688-5 gnd Immunreaktion (DE-588)4133841-8 gnd Sekretion (DE-588)4124179-4 gnd Candida albicans (DE-588)4147244-5 gnd |
| topic_facet | Neutrophiler Granulozyt Antimikrobieller Wirkstoff Immunreaktion Sekretion Candida albicans Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020429245&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ermertdavid neutrophilextracellulartrapformationinmice |