Interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors: the role in protection against hypoxia
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2010
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 88 S. Ill., graph. Darst. 21 cm |
Internformat
MARC
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| 100 | 1 | |a Müller, Lars |d 1971- |e Verfasser |0 (DE-588)140519920 |4 aut | |
| 245 | 1 | 0 | |a Interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors |b the role in protection against hypoxia |c vorgelegt von Lars Müller |
| 264 | 1 | |c 2010 | |
| 300 | |a 88 S. |b Ill., graph. Darst. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Tübingen, Univ., Diss., 2010 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020419499&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-020419499 | |
Datensatz im Suchindex
| _version_ | 1807953328412295168 |
|---|---|
| adam_text |
CONTENT
ABBREVIATIONS
4
INTRODUCTION
7
1.1.1
ENDOTHELIN
7
1.1.2
ENDOTHELIN
RECEPTORS
7
1.1.3
ENDOTHELIN
AND
ARTERIAL
BLOOD
PRESSURE
8
1.1.4
ENDOTHELIN
ACTION
IN
THE
KIDNEY
AND
LUNGS
8
1.1.5
ENDOTHELIN
ROLE
IN
CARDIOVASCULAR,
PULMONARY
AND
RENAL
DISEASES
9
1.1.6
ENDOTHELIN
ACTION
IN
THE
BRAIN
11
1.1.7
ENDOTHELIN
SIGNALLING
11
1.1.8
NEUROPROTECTIVE
EFFECTS
OF
ERYTHROPOIETIN
14
1.1.9
ERYTHROPOIETIN
SIGNALLING
15
1.2
EVIDENCE
FOR
THE
CROSSTALK
BETWEEN
EPO
AND
ENDOTHELIN
17
1.3
PURPOSE
OF
THE
STUDY
18
2.
MATERIALS
AND
METHODS
20
2.1
.
LIST
OF
MATERIALS
20
2.1.1
CELL
CULTURE
MATERIALS
20
2.1.2
IMMUNOFLUORESCENCE
MATERIALS
20
2.1.3
RNA
ISOLATION
AND
RT-PCR
MATERIALS
21
2.1.4
LDH
MEASUREMENT
21
2.1.5
MEASUREMENT
OF
EPO
21
2.1.6
MEASUREMENT
OF
HUMAN
ENDOTHELIN-1
21
2.2
.
METHODS
21
2.2.1
RAT
ASTROGLIAL
PRIMARY
CULTURE
21
2.2.2
HUMAN
ASTROCYTES
(SV-40
FHAS)
CULTURE
22
2.2.3
HYPOXIA
AND
STIMULATION
WITH
BQ123,
BQ-788
AND
EPO
22
2.3
IMMUNOCYTOCHEMICAL
ANALYSIS
23
2.4
DETERMINATION
OF
THE
CELL
VIABILITY
24
2.5
CYTOTOXICITY
ASSAY
24
2.6
RT-PCR
25
2.7
DETERMINATION
OF
ENDOTHELIN-1
26
2.8
DETERMINATION
OF
ERYTHROPOETIN
27
2.9
STATISTICAL
ANALYSES
27
3.
RESULTS
28
3.1
INFLUENCE
OF
ETA
BLOCKADE
AND
EPO
ON
NEURONS
28
3.2
INFLUENCE
OF
ETA
BLOCKADE
AND
EPO
ON
NEURAL
PRECURSORS
35
3.3
INFLUENCE
OF
BQ123
AND
EPO
ON
THE
SURVIVAL
OF
HUMAN
ASTROCYTES
36
3.4
AGE-RELATED
CHANGES
IN
RELEASE
OF
EPO
FROM
ASTROCYTES
37
3.5
INFLUENCE
OF
ETA
RECEPTOR
BLOCKADE
ON
THE
EXPRESSION
OF
EPO
39
3.6
ETA
RECEPTOR
BLOCKADE
INCREASES
THE
CONCENTRATION
OF
ET-1
RELEASED
FROM
HUMAN
ASTROCYTES
41
3.7
INFLUENCE
OF
EPO
ON
THE
RELEASE
OF
ET-1
FROM
HUMAN
ASTROCYTES
42
3.8
INFLUENCE
OF
THE
LONG
TERM
HYPOXIA
ON
THE
VIABILITY
OF
HUMAN
ASTROCYTES
44
3.9
EFFECTS
OF
ETA
RECEPTOR
BLOCKADE
ON
THE
EXPRESSION
OF
EPO
RECEPTOR
IN
ASTROCYTES
AND
NEURAL
PRECURSORS
46
4.
TABLE
SUMMARIZING
THE
RESULTS
50
5.
DISCUSSION
53
5.1
ETA
BLOCKADE
AND
EPO
INCREASES
NEURONAL
SURVIVAL
53
5.2
ETA
BLOCKADE
AND
EPO
UPREGULATES
THE
POPULATION
OF
NEURAL
PRECURSORS
54
5.3
ETA
BLOCKADE
AND
EPO
INCREASES
THE
SURVIVAL
OF
HUMAN
ASTROCYTES
55
5.4
EPO
RELEASE
FROM
ASTROCYTES
IS
AFFECTED
BY
AGING
56
5.5
ETA
RECEPTOR
BLOCKADE
UPREGULATES
THE
EXPRESSION
OF
EPO
57
5.6
ETA
RECEPTOR
BLOCKADE
INCREASES
THE
CONCENTRATION
OF
ET-1
RELEASED
FROM
HUMAN
ASTROCYTES
58
5.7
EPO
DECREASES
THE
RELEASE
OF
ET-1
FROM
HUMAN
ASTROCYTES
60
5.8
LONG
TERM
(48H
AND
72H)
BUT
NOT
24H
HYPOXIA
CAUSES
CELL
DEATH
IN
HUMAN
ASTROCYTES
CULTURE
61
5.9
ETA
RECEPTOR
BLOCKADE
AFFECTS
THE
EXPRESSION
OF
EPO
RECEPTOR
IN
ASTROCYTES
AND
NEURAL
PRECURSORS
62
6.
SUMMARY
65
7.
REFERENCES
66
8.
ACKNOWLEDGMENTS
87
9.
CURRICULUM
VITAE
88 |
| any_adam_object | 1 |
| author | Müller, Lars 1971- |
| author_GND | (DE-588)140519920 |
| author_facet | Müller, Lars 1971- |
| author_role | aut |
| author_sort | Müller, Lars 1971- |
| author_variant | l m lm |
| building | Verbundindex |
| bvnumber | BV036497000 |
| ctrlnum | (OCoLC)560576022 (DE-599)BVBBV036497000 |
| dewey-full | 612.01 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 612 - Human physiology |
| dewey-raw | 612.01 |
| dewey-search | 612.01 |
| dewey-sort | 3612.01 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Thesis Book |
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| language | English |
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| physical | 88 S. Ill., graph. Darst. 21 cm |
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| spelling | Müller, Lars 1971- Verfasser (DE-588)140519920 aut Interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors the role in protection against hypoxia vorgelegt von Lars Müller 2010 88 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Tübingen, Univ., Diss., 2010 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020419499&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Müller, Lars 1971- Interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors the role in protection against hypoxia |
| subject_GND | (DE-588)4113937-9 |
| title | Interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors the role in protection against hypoxia |
| title_auth | Interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors the role in protection against hypoxia |
| title_exact_search | Interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors the role in protection against hypoxia |
| title_full | Interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors the role in protection against hypoxia vorgelegt von Lars Müller |
| title_fullStr | Interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors the role in protection against hypoxia vorgelegt von Lars Müller |
| title_full_unstemmed | Interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors the role in protection against hypoxia vorgelegt von Lars Müller |
| title_short | Interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors |
| title_sort | interplay between endothelin and erythropoietin in astroglial cells and neuronal progenitors the role in protection against hypoxia |
| title_sub | the role in protection against hypoxia |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020419499&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mullerlars interplaybetweenendothelinanderythropoietininastroglialcellsandneuronalprogenitorstheroleinprotectionagainsthypoxia |