Post-transcriptional and post-translational regulation of the Sucrose Transporter SUT4 from Solanaceae:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Göttingen
Cuvillier
2010
|
| Ausgabe: | 1. Aufl. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IX, 149 S. Ill., graph. Darst. |
| ISBN: | 9783869553412 |
Internformat
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS ABSTRACT I ZUSAMMENFASSUNG II TABLE OF CONTENTS ILL
ABBREVIATIONS AND ACRONYMS VII 1 INTRODUCTION 1 1.1 PHYLOGENETIC AND
PHYSIOLOGIC ANALYSIS OF SUCROSE TRANSPORTERS IN PLANTS 1 1.1.1
PHYLOGENETIC ANALYSIS OF SUCROSE TRANSPORTERS IN PLANT KINGDOM 1 1.1.2
SUCROSE TRANSPORTERS IN SOLANACEOUS PLANTS 3 1.2 PHLOEM MOBILE MRNAS AND
PROTEINS 6 1.2.1 PHLOEM MOBILE MRNAS 7 1.2.2 PHLOEM MOBILE PROTEINS 9
1.3 MRNA STABILITY AND RNA-BINDING PROTEINS 11 1.3.1 DETERMINANTS OF
MRNA STABILITY IN PLANTS 11 1.3.2 THE ROLE OF STRESSES IN REGULATING
MRNA STABILITY 12 1.3.3 RNA-BINDING PROTEINS IN PLANTS 13 1.4 LIGHT
DEPENDENT SHADE AVOIDANCE RESPONSES IN PLANTS 14 1.5 METHODS TO
INVESTIGATE PROTEIN-PROTEIN OR RNA-PROTEIN INTERACTIONS 16 1.5.1 THE
SPLIT-UBIQUITIN MEMBRANE YEAST TWO-HYBRID ASSAY IDENTIFY PROTEIN
INTERACTION PARTNER IN A UNBIASED MANNER 16 1.5.2 BIFC SYSTEM SERVES AS
AN EFFICIENT TECHNIQUE FOR IDENTIFICATION OF PROTEIN-PROTEIN INTERACTION
PARTNERS 17 1.5.3 CO-IMMUNOPRECIPITATION AND TANDEM AFFINITY
PURIFICATION TECHNIQUES. 18 1.5.4 YEAST THREE-HYBRID ASSAY TO IDENTIFY
RNA BINDING PROTEINS 18 1.6 THE GOALS OF THE STUDY 20 2 MATERIALS AND
METHODS 21 2.1 MATERIALS 21 2.1.1 CHEMICALS AND ENZYMES 21 2.1.1.1
CHEMICALS 21 2.1.1.2 ENZYMES AND ANTIBODIES 21 2.1.2 BACTERIAL STRAINS
22 2.1.3 YEAST STRAIN 22 2.1.4 CDNA LIBRARY 22 2.1. BIBLIOGRAFISCHE
INFORMATIONEN HTTP://D-NB.INFO/1003246672 DIGITALISIERT DURCH 2.3.5
GENOMIC DNA ISOLATION 30 2.3.6 RNA ISOLATION AND REVERSE TRANSCRIPTION
31 2.3.7 RNA QUANTIFICATION BY REAL-TIME RT-PCR 31 2.3.8 NORTHERN BLOT
32 2.3.9 PROTEIN EXTRACTION AND DETECTION 32 2.3.9.1 PROTEIN
PURIFICATION FROM S1SUT4 TOBACCO 32 2.3.9.2 MICROSOMAL FRACTION
ISOLATION 32 2.3.9.3 PLASMA MEMBRANE FRACTION ISOLATION 33 2.3.9.4 BCA
PROTEIN ASSAY (THERMO SCIENTIFIC) 33 2.3.9.5 WESTERN BLOT 34 2.3.10 BIFC
IN LIVING TOBACCO CELLS 34 2.4 YEAST RELATED EXPERIMENTS 35 2.4.1
PREDICTION OF STSUT4 RNA STRUCTURES 35 2.4.2 THE CONSTRUCT OF HYBRID RNA
MOLECULE 35 2.4.3 CONSTRUCT OF CDNA LIBRARY 36 2.4.4 OUTLINE OF WORKING
ROUTE OF YEAST THREE-HYBRID ASSAY 37 2.4.5 X-GAL ANALYSIS OF INTERACTION
CLONES 39 2.4.6 REDUCE NON-SPECIFIC TRANSACTIVATION OF THE HIS3 REPORTER
GENE 39 2.4.7 SMALL SCALE YEAST TRANSFORMATION 40 2.4.8 LARGE SCALE
YEAST TRANSFORMATION 40 2.4.9 ISOLATION OF PLASMID DNA FROM YEAST CELLS
41 2.5 REGULATION OF HALF LIFE OF SUCROSE TRANSPORTER MRNAS 42 2.5.1
INFLUENCES ON SUT4 UNDER FAR-RED LIGHT ENRICHMENT 42 2.5.2 INHIBITOR
STUDIES AND FEEDING EXPERIMENTS 42 2.6 PHYSIOLOGICAL ANALYSIS OF
TRANSGENIC POTATO AND TOBACCO PLANTS 42 2.6.1 REAL-TIME MONITORING OF
ETHYLENE (ET) PRODUCTION 42 2.6.2 IN VITRO POLLEN GERMINATION ASSAY 43
2.6.3 POLLEN VIABILITY ASSAY 43 2.6.4 SOLUBLE SUGAR CONTENT AND STARCH
MEASUREMENT 44 2. 3.3.3.3 TUBER YIELD OF TRANSGENIC STSUT2&STSUT4-RNAI
POTATO PLANTS 66 3.3.3.4 TUBER DORMANCY AND SPROUTING 68 3.4 SUT4
OVER-EXPRESSION IN TOBACCO PLANTS 69 3.4.1 CONFIRMATION OF TOBACCO
TRANSFORMATION 70 3.4.1.1 RECOMBINANT PN-TAPA S1SUT4 CONSTRUCT 70
3.4.1.2 CONFIRMATION OF SUCCESSFUL TRANSFORMATION 70 3.4.2 SISUT4 MRNA
ACCUMULATION IN N-TAPA SISUT4 02 TOBACCO 71 3.4.3 S1SUT4 PROTEIN LEVEL
IN N-TAPA S1SUT4 TOBACCO 72 3.4.4 PHENOTYPES OF N-TAPA S1SUT4 TOBACCO
PLANTS 74 3.4.5 SUT4 IN SOLANACEOUS PLANTS IS A PLASMA MEMBRANE PROTEIN
78 3.5 THE EFFECTS ON GENES INVOLVED IN FLOWER INDUCTION AND
TUBERIZATION IN STSUT4-RNAI POTATO PLANTS 79 3.5.1 STSUT4 ACCUMULATION
WAS INDUCED UNDER FR LIGHT ENRICHMENT 80 3.5.2 INCREASED STSUT4 MRNA
ACCUMULATION WAS CAUSED BY PROLONGED HALF LIFE OF TRANSCRIPTS UNDER FR
ENRICHMENT 81 3.5.3 FAR-RED LIGHT AFFECTS STSUT4 AND SUAR1 ACCUMULATION
82 3.5.4 THE EXPRESSION OF GIGANTEA (GI) IN STSUT4-RNAI POTATO PLANTS 84
3.5.5 THE EXPRESSION OF DIFFERENT FLOWERING GENES IN STSUT4-RNAI POTATO
PLANTS UNDER SD CONDITION 85 3.6 CONFIRMATION OF SUT4 PROTEIN-PROTEIN
INTERACTION PARTNERS 87 3.6.1 THE CONFIRMATION OF PROTEIN DISULFIDE
ISOMERASE (PDI) INTERACTING WITH SUT 4.3.3 STSUT4 IS INVOLVED IN SHADE
AVOIDANCE RESPONSES 107 4.3.4 SUT4 SERVES AS AN COMPONENT IN POTATO
FLOWERING AND TUBERIZATION PATHWAYS 109 4.4 THE ROLE OF STSUT2 IN POTATO
PLANTS 111 4.5 REGULATION OF SUCROSE TRANSPORTER PROTEIN STABILITY AND
LOCALIZATION 112 4.5.1 PDI MIGHT BE INVOLVED IN PROTEIN FOLDING AND
LOCALIZATION OF SUCROSE TRANSPORTERS 112 4.5.2 SUT4 IS INVOLVED IN
ETHYLENE SIGNALING PATHWAY THROUGH INTERACTION WITH ETR, AN ETHYLENE
RECEPTOR 113 4.6 YEAST THREE-HYBRID SYSTEM FOR IDENTIFICATION OF
RNA-PROTEIN INTERACTIONS .. 114 4.6.1 HYBRID RNA (BAIT VECTORS) 114
4.6.2 DIFFERENCES OF VP16 AND GAL4 ACTIVATION DOMAIN 115 4.6.3
IDENTIFICATION OF SEQUENCE SPECIFIC RNA BINDING PROTEINS 116 4.6.4
LIMITATIONS OF YEAST THREE-HYBRID ASSAY 117 5 CONCLUSION 121 6 APPENDIX
123 6.1 MAP OF VECTORS USED IN THIS STUDY 123 6.2 SECONDARY STRUCTURE OF
DIFFERENT LENGTH OF STSUT4 MRNAS 125 7 REFERENCES 129 8 ACKNOWLEDGEMENT
145 PUBLICATION 147 VI
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| any_adam_object | 1 |
| author | He, Hongxia 1977- |
| author_GND | (DE-588)141471743 |
| author_facet | He, Hongxia 1977- |
| author_role | aut |
| author_sort | He, Hongxia 1977- |
| author_variant | h h hh |
| building | Verbundindex |
| bvnumber | BV036489910 |
| classification_rvk | WL 9325 |
| ctrlnum | (OCoLC)665158565 (DE-599)BVBBV036489910 |
| dewey-full | 572.5654 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.5654 |
| dewey-search | 572.5654 |
| dewey-sort | 3572.5654 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| spelling | He, Hongxia 1977- Verfasser (DE-588)141471743 aut Post-transcriptional and post-translational regulation of the Sucrose Transporter SUT4 from Solanaceae Hongxia He 1. Aufl. Göttingen Cuvillier 2010 IX, 149 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Berlin, Humboldt-Univ., Diss., 2010 Carrier-Proteine (DE-588)4213127-3 gnd rswk-swf Posttranskriptionelle Regulation (DE-588)4356177-9 gnd rswk-swf Saccharose (DE-588)4178816-3 gnd rswk-swf Posttranslationale Änderung (DE-588)4204069-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Saccharose (DE-588)4178816-3 s Carrier-Proteine (DE-588)4213127-3 s Posttranskriptionelle Regulation (DE-588)4356177-9 s Posttranslationale Änderung (DE-588)4204069-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020412529&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | He, Hongxia 1977- Post-transcriptional and post-translational regulation of the Sucrose Transporter SUT4 from Solanaceae Carrier-Proteine (DE-588)4213127-3 gnd Posttranskriptionelle Regulation (DE-588)4356177-9 gnd Saccharose (DE-588)4178816-3 gnd Posttranslationale Änderung (DE-588)4204069-3 gnd |
| subject_GND | (DE-588)4213127-3 (DE-588)4356177-9 (DE-588)4178816-3 (DE-588)4204069-3 (DE-588)4113937-9 |
| title | Post-transcriptional and post-translational regulation of the Sucrose Transporter SUT4 from Solanaceae |
| title_auth | Post-transcriptional and post-translational regulation of the Sucrose Transporter SUT4 from Solanaceae |
| title_exact_search | Post-transcriptional and post-translational regulation of the Sucrose Transporter SUT4 from Solanaceae |
| title_full | Post-transcriptional and post-translational regulation of the Sucrose Transporter SUT4 from Solanaceae Hongxia He |
| title_fullStr | Post-transcriptional and post-translational regulation of the Sucrose Transporter SUT4 from Solanaceae Hongxia He |
| title_full_unstemmed | Post-transcriptional and post-translational regulation of the Sucrose Transporter SUT4 from Solanaceae Hongxia He |
| title_short | Post-transcriptional and post-translational regulation of the Sucrose Transporter SUT4 from Solanaceae |
| title_sort | post transcriptional and post translational regulation of the sucrose transporter sut4 from solanaceae |
| topic | Carrier-Proteine (DE-588)4213127-3 gnd Posttranskriptionelle Regulation (DE-588)4356177-9 gnd Saccharose (DE-588)4178816-3 gnd Posttranslationale Änderung (DE-588)4204069-3 gnd |
| topic_facet | Carrier-Proteine Posttranskriptionelle Regulation Saccharose Posttranslationale Änderung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020412529&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT hehongxia posttranscriptionalandposttranslationalregulationofthesucrosetransportersut4fromsolanaceae |