Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Jülich
Forschungszentrum, Zentralbibliothek
2010
|
| Schriftenreihe: | Schriften des Forschungszentrums Jülich
Reihe Gesundheit ; Bd. 21 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 120 S. Ill., graph. Darst. 24 cm |
| ISBN: | 9783893366071 |
Internformat
MARC
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| 020 | |a 9783893366071 |c kart. |9 978-3-89336-607-1 | ||
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| 100 | 1 | |a Hanke, Tanja |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H |c Tanja Hanke. Forschungszentrum Jülich GmbH, Institute of Biotechnology (IBT-1). [Hrsg.: Forschungszentrum Jülich GmbH, Zentralbibliothek] |
| 264 | 1 | |a Jülich |b Forschungszentrum, Zentralbibliothek |c 2010 | |
| 300 | |a 120 S. |b Ill., graph. Darst. |c 24 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Schriften des Forschungszentrums Jülich : Reihe Gesundheit |v Bd. 21 | |
| 502 | |a Zugl.: Düsseldorf, Univ., Diss., 2010 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 830 | 0 | |a Schriften des Forschungszentrums Jülich |v Reihe Gesundheit ; Bd. 21 |w (DE-604)BV035800060 |9 21 | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-020410481 | |
Datensatz im Suchindex
| _version_ | 1812452857031426048 |
|---|---|
| adam_text |
CONTENTS
I
ABBREVIATIONS
.
1
II
INTRODUCTION
.
3
III
MATERIALS
AND
METHODS
.
11
1.
BACTERIAL
STRAINS
.
11
2.
PLASMIDS
AND
OLIGONUCLEOTIDES
.
12
3.
CHEMICALS
AND
ENZYMES
.
14
4.
MEDIA
.
15
5.
CULTURE
CONDITIONS
OF
G.
OXYDANS
AND
E.
COLI
.
15
6.
DETERMINATION
OF
CELL
DRY
WEIGHT
.
17
7.
STOCK
CULTURES
.
17
8.
MOLECULAR
BIOLOGICAL
METHODS
.
17
8.1
ISOLATION
OFDNA
.
17
8.2
RECOMBINANT
DNA-TECHNIQUES
.
18
8.3
POLYMERASE
CHAIN
REACTION
(PCR)
.
18
8.4
AGAROSE
GEL
ELECTROPHORESIS
.
19
8.5
TRANSFORMATION
OFE.
COLI
AND
G.
OXYDANS
.
19
8.6
OVEREXPRESSION
OF
THE
G.
OXYDANS
CCP
GENE
ENCODING
CYTOCHROME
C
PEROXIDASE
.
20
8.7
CONSTRUCTION
OF
MARKER-FREE
DELETION
MUTANTS
.
21
8.8
RNA
PREPARATION
.
21
8.9
CDNA
LABELING
AND
RT
PCR
.
22
8.10
G.
OXYDANS
DNA
MICROARRAYS
.
22
9.
BIOCHEMICAL
METHODS
.
23
9.1
CELL
DISRUPTION,
PREPARATION
OF
CRUDE
EXTRACTS
AND
MEMBRANE
FRACTIONS.
23
9.2
DETERMINATION
OF
PROTEIN
CONCENTRATION.24
9.3 POLYACRYLAMIDE
GEL
ELECTROPHORESIS
OF
PROTEINS
(SDS-PAGE)
.24
9.4
PROTEIN
PURIFICATION
BY
COLUMN
CHROMATOGRAPHY
.24
9.5
DETERMINATION
OF
OXYGEN
CONSUMPTION
RATES
WITH
A
CLARK
ELECTRODE
.
26
9.6
DETERMINATION
OF
ENZYME
ACTIVITIES
.
26
9.7
CONVERSION
OF
INACTIVE
ALCOHOL
DEHYDROGENASE
TO
ACTIVE
ENZYME
IN
RESTING
CELLS
.30
10.
BIOANALYTICAL
METHODS
.
30
10.1
SAMPLING
AND
SAMPLE
PROCESSING
FOR
LC-MS
ANALYSIS
.
30
10.2
DETERMINATION
OF
METABOLITES
BY
HIGH
PERFORMANCE
LIQUID
CHROMATOGRAPHY
(HPLC)
.
31
10.3
13
C
METABOLIC
FLUX
ANALYSIS
.
31
10.4
MALDI-TOF-MASS
SPECTROMETRY.
.
32
IV
RESULTS
.
33
1.
CHARACTERISATION
OF
THE
DELETION
MUTANT
G.
OXYDANS
621
H-AQCRABC
.
33
2.
GENOME-WIDE
TRANSCRIPTION
ANALYSES
.
51
3.
1S
C-METABOIOME
ANALYSIS
AND
FLUX
ANALYSIS
(MFA)
.
63
V
DISCUSSION
.
73
1.
ANALYSIS
OF
PHYSIOLOGICAL
AND
METABOLIC
FUNCTIONS
OF
THE
CYTOCHROME
BEY
COMPLEX
IN
G.
OXYDANS
.
73
2.
DIFFERENTIAL
GENE
REGULATION
AT
OXYGEN
LIMITATION
AND
AT
LOW
PH
.
80
3.
CHARACTERISATION
OF
GROWTH
OF
G.
OXYDANS
621H
ON
GLUCOSE
WITH
MICRO
ARRAY-,
'
3
C-METABOLOME
AND
FLUX-ANALYSIS
.
84
VI
REFERENCES
.
89
VII
APPENDIX
.
101 |
| any_adam_object | 1 |
| author | Hanke, Tanja |
| author_facet | Hanke, Tanja |
| author_role | aut |
| author_sort | Hanke, Tanja |
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| building | Verbundindex |
| bvnumber | BV036487824 |
| ctrlnum | (OCoLC)614493108 (DE-599)DNB1001667360 |
| dewey-full | 572.472933 |
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| dewey-search | 572.472933 |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| id | DE-604.BV036487824 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T16:02:19Z |
| institution | BVB |
| isbn | 9783893366071 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020410481 |
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| physical | 120 S. Ill., graph. Darst. 24 cm |
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| series | Schriften des Forschungszentrums Jülich |
| series2 | Schriften des Forschungszentrums Jülich : Reihe Gesundheit |
| spelling | Hanke, Tanja Verfasser aut Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H Tanja Hanke. Forschungszentrum Jülich GmbH, Institute of Biotechnology (IBT-1). [Hrsg.: Forschungszentrum Jülich GmbH, Zentralbibliothek] Jülich Forschungszentrum, Zentralbibliothek 2010 120 S. Ill., graph. Darst. 24 cm txt rdacontent n rdamedia nc rdacarrier Schriften des Forschungszentrums Jülich : Reihe Gesundheit Bd. 21 Zugl.: Düsseldorf, Univ., Diss., 2010 (DE-588)4113937-9 Hochschulschrift gnd-content Schriften des Forschungszentrums Jülich Reihe Gesundheit ; Bd. 21 (DE-604)BV035800060 21 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020410481&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hanke, Tanja Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H Schriften des Forschungszentrums Jülich |
| subject_GND | (DE-588)4113937-9 |
| title | Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H |
| title_auth | Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H |
| title_exact_search | Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H |
| title_full | Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H Tanja Hanke. Forschungszentrum Jülich GmbH, Institute of Biotechnology (IBT-1). [Hrsg.: Forschungszentrum Jülich GmbH, Zentralbibliothek] |
| title_fullStr | Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H Tanja Hanke. Forschungszentrum Jülich GmbH, Institute of Biotechnology (IBT-1). [Hrsg.: Forschungszentrum Jülich GmbH, Zentralbibliothek] |
| title_full_unstemmed | Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H Tanja Hanke. Forschungszentrum Jülich GmbH, Institute of Biotechnology (IBT-1). [Hrsg.: Forschungszentrum Jülich GmbH, Zentralbibliothek] |
| title_short | Studies on central carbon metabolism and respiration of Gluconobacter oxydans 621H |
| title_sort | studies on central carbon metabolism and respiration of gluconobacter oxydans 621h |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020410481&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035800060 |
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