Interaction of human primary keratinocytes with toll-like receptor ligands and resulting pro-inflammatory signals:
Saved in:
| Main Author: | |
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20100120-813357-1-1 Inhaltsverzeichnis |
| Physical Description: | 115 S. Ill., graph. Darst. |
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Record in the Search Index
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| adam_text | IMAGE 1
TECHNISCHE UNIVERSITAET MUENCHEN
LEHRSTUHL FUER CHEMISCH-TECHNISCHE ANALYSE UND CHEMISCHE
LEBENSMITTELTECHNOLOGIE
INTERACTION OF HUMAN PRIMARY KERATINOCYTES WITH
TOLL-LIKE RECEPTOR LIGANDS AND RESULTING PRO- INFLAMMATORY SIGNALS
BEHNAM NADERI KALALI
VOLLSTAENDIGER ABDRUCK DER VON DER FAKULTAET WISSENSCHAFTSZENTRUM
WEIHENSTEPHAN FUER ERNAEHRUNG, LANDNUTZUNG UND UMWELT DER TECHNISCHEN
UNIVERSITAET MUENCHEN ZUR
ERLANGUNG DES AKADEMISCHEN GRADES EINES
DOKTORS DER NATURWISSENSCHAFTEN
GENEHMIGTE DISSERTATION.
VORSITZENDER: UNIV.- PROF. DR. W. HUBER
PRUEFER DER DISSERTATION:
1. UNIV.- PROF. DR. DR. H.C. H. PARLAR
2. UNIV.- PROF. DR. M. W. OLLERT
DIE DISSERTATION WURDE AM 22.09.2009 BEI DER TECHNISCHEN UNIVERSITAET
MUENCHEN
EINGEREICHT UND DURCH DIE FAKULTAET WISSENSCHAFTSZENTRUM WEIHENSTEPHAN
FUER
ERNAEHRUNG, LANDNUTZUNG UND UMWELT AM 20.01.2010 ANGENOMMEN.
2010
B
20 560
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1003108679
DIGITALISIERT DURCH
IMAGE 2
LIST OF CONTENTS
1. LIST OF CONTENTS
1. LIST OF CONTENTS 1
2. LIST OF ABBREVIATIONS 4
3. INTRODUCTION 5
3.1. SKIN 5
3.1.1. SKIN A PHYSICAL BARRIER 8
3.1.2. SKIN IMMUNE SYSTEM 8
3.2. LANGERHANS CELLS 9
3.3. KERATINOCYTES 10
3.4. TOLL-LIKE RECEPTORS (TLRS) 10
3.4.1. TLR1/2/6 12
3.4.2. TLR3 13
3.4.3. TLR4 16
3.4.4. TLR5 17
3.4.5. TLR7/8 17
3.4.6. TLR9 18
3.4.7. TLR10 19
3.5. TLRS SIGNALLING PATHWAY 20
3.5.1. MYD88 20
3.6. CONSEQUENCES OF TLR ACTIVATION 22
3.7. AIM OF THE STUDY 24
4. METHODS AND MATERIALS 25
4.1. CELL CULTURE AND REAGENTS 25
4.2. CYTOKINE AND CHEMOKINE IMMUNOASSAYS 26
4.3. MICROARRAY ANALYSIS 27
4.4. SEPARATION OF KERATINOCYTES INTO BASAL AND SUPRABASAL LAYER CELLS
28
4.5. FACS ANALYSIS OF CELL SURFACE MARKERS AND PURIFICATION FOR MDCS 28
4.6. PREPARATION OF MDCS 29
4.7. RNA INHIBITION 29
4.8. RNA ISOLATION 30
4.9. RNA QUANTIFICATION 31
IMAGE 3
LIST OF CONTENTS
4.10. RT-PCR 31
4.11. REAL-TIME PCR 32
4.12. IMMUNOFLUORESCENCE STAINING 35
4.13. RELA AND IRF3 NUCLEAR TRANSLOCATION ANALYSIS 35
4.14. CELL LYSIS, GEL ELECTROPHORESIS AND IMMUNOBLOTTING 36
4.15. GARDIQUIMOD TREATMENT AND CELL VIABILITY 37
5. RESULTS 38
5.1. EXPRESSION OF TOLL-LIKE RECEPTORS IN HUMAN EPIDERMIS AND CULTURED
KERATINOCYTES 38
5.2. TLR-EXPRESSION ON DENDRITIC CELLS FROM ATOPIC VERSUS NON-ATOPIC
DONORS .40
5.3. MDCS SURFACE RECEPTOR EXPRESSION PROFILE AND RESPONSE TO
TLR-LIGANDS41
5.4. EXPRESSION OF THE DSRNA RECOGNIZING RECEPTORS PKR AND RIG IN MDCS
.45
5.5. DIFFERENTIAL IL-8 INDUCTION BY VARIOUS TLR LIGANDS IN CULTURED
HUMAN
KERATINOCYTES 46
5.6. NF-KB INDUCTION BY TLR LIGANDS IN CULTURED HUMAN KERATINOCYTES 49
5.7. ROLE OF LPS AND TLR4 IN CULTURED HUMAN KERATINOCYTES 51
5.8. GENOME-WIDE ANALYSIS OF CHANGES IN KERATINOCYTE GENE EXPRESSION IN
RESPONSE TO POLY (I:C) STIMULATION 53
5.9. ALL DSRNA RECEPTORS ARE EXPRESSED IN PRIMARY KERATINOCYTES 61
5.10. THE MAJOR DSRNA RECOGNIZING AND SIGNALING PATHWAYS ARE FUNCTIONAL
IN
PRIMARY HUMAN KERATINOCYTES 65
5.11. INHIBITION OF DSRNA SIGNALLING PATHWAYS 66
5.12. TLR7, THE RECEPTOR FOR IMIDAZOQUINOLINES AND SINGLE-STRANDED RNA,
IS
FUNCTIONALLY UP-REGULATED IN POLY (L:C)-STIMULATED KERATINOCYTES 70
6. DISCUSSION 75
7. REFERENCES 87
8. LIST OF FIGURES 106
9. LIST OF TABLES 108
10. ACKNOWLEDGMENT 109
11. SUMMARY 111
12. CURRICULUM VITAE 112
|
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| author | Naderi Kalali, Behnam 1972- |
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| dewey-full | 612.7 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 612 - Human physiology |
| dewey-raw | 612.7 |
| dewey-search | 612.7 |
| dewey-sort | 3612.7 |
| dewey-tens | 610 - Medicine and health |
| discipline | Chemie Medizin |
| format | Thesis Book |
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| spelling | Naderi Kalali, Behnam 1972- Verfasser (DE-588)140998101 aut Interaction of human primary keratinocytes with toll-like receptor ligands and resulting pro-inflammatory signals Behnam Naderi Kalali 2010 115 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2010 Keratinozyt (DE-588)4293091-1 gnd rswk-swf Immunreaktion (DE-588)4133841-8 gnd rswk-swf Toll-like-Rezeptoren (DE-588)4748432-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Keratinozyt (DE-588)4293091-1 s Immunreaktion (DE-588)4133841-8 s Toll-like-Rezeptoren (DE-588)4748432-9 s b DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:91-diss-20100120-813357-1-1 https://mediatum.ub.tum.de/node?id=813357 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20100120-813357-1-1 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020304466&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Naderi Kalali, Behnam 1972- Interaction of human primary keratinocytes with toll-like receptor ligands and resulting pro-inflammatory signals Keratinozyt (DE-588)4293091-1 gnd Immunreaktion (DE-588)4133841-8 gnd Toll-like-Rezeptoren (DE-588)4748432-9 gnd |
| subject_GND | (DE-588)4293091-1 (DE-588)4133841-8 (DE-588)4748432-9 (DE-588)4113937-9 |
| title | Interaction of human primary keratinocytes with toll-like receptor ligands and resulting pro-inflammatory signals |
| title_auth | Interaction of human primary keratinocytes with toll-like receptor ligands and resulting pro-inflammatory signals |
| title_exact_search | Interaction of human primary keratinocytes with toll-like receptor ligands and resulting pro-inflammatory signals |
| title_full | Interaction of human primary keratinocytes with toll-like receptor ligands and resulting pro-inflammatory signals Behnam Naderi Kalali |
| title_fullStr | Interaction of human primary keratinocytes with toll-like receptor ligands and resulting pro-inflammatory signals Behnam Naderi Kalali |
| title_full_unstemmed | Interaction of human primary keratinocytes with toll-like receptor ligands and resulting pro-inflammatory signals Behnam Naderi Kalali |
| title_short | Interaction of human primary keratinocytes with toll-like receptor ligands and resulting pro-inflammatory signals |
| title_sort | interaction of human primary keratinocytes with toll like receptor ligands and resulting pro inflammatory signals |
| topic | Keratinozyt (DE-588)4293091-1 gnd Immunreaktion (DE-588)4133841-8 gnd Toll-like-Rezeptoren (DE-588)4748432-9 gnd |
| topic_facet | Keratinozyt Immunreaktion Toll-like-Rezeptoren Hochschulschrift |
| url | https://mediatum.ub.tum.de/node?id=813357 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20100120-813357-1-1 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020304466&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT naderikalalibehnam interactionofhumanprimarykeratinocyteswithtolllikereceptorligandsandresultingproinflammatorysignals |