MicroRNA expression detection methods:
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Berlin [u.a.]
Springer
2010
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XX, 387 S. Ill., graph. Darst. |
| ISBN: | 9783642049279 |
Staff View
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| adam_text | Titel: MicroRNA expression detection methods
Autor: Wang, Zhiguo
Jahr: 2010
Contents
Part I General Remarks
1 Detection, Profiling, and Quantification of miRNA Expression ....... 3
1.1 miRNA Biology ....................................................... 3
1.1.1 Biogenesis of miRNAs ......................................... 3
1.1.2 Actions of miRNAs ............................................. 7
1.2 Human Disease-Related Expression Profiles of miRNAs ............. 9
1.2.1 Spatiotemporal Expression Profiles ............................ 9
1.2.2 miRNA Transcriptome and Human Physiology .............. 17
1.2.3 miRNA Transcriptome in Diseased States .................... 20
1.3 miRNAs as Biomarkers for Human Disease ......................... 37
1.4 Methods for Analyzing miRNAs Expression ........................ 38
1.4.1 Ideal Methods for miRNA Detection .......................... 39
1.4.2 Classification of Methods for miRNA Detection ............. 39
1.4.3 Brief Introduction to the Currently Available miRNA
Detection Methods ............................................. 40
References ................................................................. 53
Part II miRNA Microarray Methods
2 Microarray and Its Variants for miRNA Profiling ................... 67
2.1 Introduction ........................................................... 68
2.2 Protocol ............................................................... 70
2.2.1 Materials ....................................................... 70
2.2.2 Instruments ..................................................... 71
2.2.3 Reagents ....................................................... 72
2.2.4 Procedures ..................................................... 73
2.3 Application and Limitation ........................................... 77
References ................................................................. 78
xii Contents
Part ni Northern Blotting Methods
3 Northern Blotting and Its Variants for Detecting
Expression and Analyzing Tissue Distribution of miRNAs ........... 83
3.1 Introduction ........................................................... 83
3.2 Protocol ............................................................... 85
3.2.1 Basic Protocols ................................................ 85
3.2.2 Protocols with Improved Sensitivity .......................... 89
3.2.3 Protocols with Improved Specificity and Sensitivity .......... 91
3.2.4 Protocols with Nonisotopic Detection ......................... 97
3.3 Application and Limitation ........................................... 99
References ................................................................. 99
Part IV In Situ Hybridization Methods
4 In Situ Hybridization and Its Variants for Detecting
Expression and Analyzing Cellular Distribution of miRNAs ....... 103
4.1 Introduction ......................................................... 104
4.2 Protocol ............................................................. 106
4.2.1 Basic Protocol ................................................ 106
4.2.2 LNA-Modified Protocol with Enhanced Sensitivity
and Specificity ................................................ 116
4.2.3 Ultramer Extension Protocol with Reduced Stringency
and Expense ................................................... 122
4.3 Application and Limitation ......................................... 126
References ................................................................ 127
Part V Real-Time RT-PCR Methods
5 End-Point Stem-Loop Real-Time RT-PCR for miRNA
Quantification ........................................................... 131
5.1 Introduction ......................................................... 132
5.2 Protocol ............................................................. 134
5.2.1 Materials ...................................................... 134
5.2.2 Instruments .................................................... 135
5.2.3 Reagents ...................................................... 135
5.2.4 Procedures .................................................... 135
5.3 Application and Limitation ......................................... 139
References ................................................................ 139
Contents xiii
6 miR-Q RT-PCR for miRNA Quantification .......................... 141
6.1 Introduction ......................................................... 141
6.2 Protocol ............................................................. 142
6.2.1 Materials ...................................................... 142
6.2.2 Instruments .................................................... 142
6.2.3 Reagents ...................................................... 143
6.2.4 Procedures .................................................... 143
6.3 Application and Limitation ......................................... 146
References ................................................................ 146
7 Poly(A)-Tailed Universal Reverse Transcription ..................... 147
7.1 Introduction ......................................................... 147
7.2 Protocol ............................................................. 148
7.2.1 Materials ...................................................... 148
7.2.2 Instalments .................................................... 149
7.2.3 Reagents ...................................................... 149
7.2.4 Procedures .................................................... 149
7.3 Application and Limitation ......................................... 151
References ................................................................ 151
8 Multiplexing RT-PCR for High-Throughput
miRNA Profiling ........................................................ 153
8.1 Introduction ......................................................... 154
8.2 Protocol ............................................................. 155
8.2.1 Materials ...................................................... 155
8.2.2 Instruments .................................................... 155
8.2.3 Reagents ...................................................... 155
8.2.4 Procedures .................................................... 155
8.3 Application and Limitation ......................................... 156
References ................................................................ 157
9 miRNA Amplification Profiling (mRAP) .............................. 159
9.1 Introduction ......................................................... 159
9.2 Protocol ............................................................. 160
9.2.1 Materials ...................................................... 160
9.2.2 Instruments .................................................... 162
9.2.3 Reagents ...................................................... 163
9.2.4 Procedures .................................................... 164
9.3 Application and Limitation ......................................... 169
References ................................................................ 169
xiv Contents
Part VI Cloning Methods
10 miRNA Serial Analysis of Gene Expression
(miRAGE or SAGE) .................................................... 173
10.1 Introduction ........................................................ 173
10.2 Protocol ............................................................ 174
10.2.1 Materials ................................................... 174
10.2.2 Instruments ................................................ 176
10.2.3 Reagnets ................................................... 176
10.2.4 Procedures ................................................. 177
10.3 Application and Limitation ........................................ 187
References ................................................................ 188
Part VII Nanoparticle Methods
11 Electrocatalytic Nanoparticle Tags Technique
for High-Sensitivity miRNA Expression Analysis .................... 191
11.1 Introduction ........................................................ 191
11.2 Protocol ............................................................ 194
11.2.1 Materials ................................................... 194
11.2.2 Instruments ................................................ 194
11.2.3 Reagents ................................................... 194
11.2.4 Procedures ................................................. 195
11.3 Application and Limitation ........................................ 197
References ................................................................ 197
12 Nanoparticle-Amplified SPR Imaging for
High-Sensitivity miRNA Profiling ..................................... 199
12.1 Introduction ........................................................ 199
12.2 Protocol ............................................................ 201
12.2.1 Materials ................................................... 201
12.2.2 Instruments ................................................ 201
12.2.3 Reagents ................................................... 202
12.2.4 Procedures ................................................. 202
12.3 Application and Limitation ........................................ 205
References ................................................................ 206
13 Conducting Polymer Nanowires Technique for
High-Sensitivity miRNA Expression Analysis ........................ 207
13.1 Introduction ........................................................ 207
13.2 Protocol ............................................................ 209
13.2.1 Materials ................................................... 209
13.2.2 Instruments ................................................ 209
Contents xv
13.2.3 Reagents ................................................... 209
13.2.4 Procedures ................................................. 211
13.3 Application and Limitation ........................................ 214
References ................................................................ 215
14 Gold Nanoparticle Probe Method for miRNA
Quantification ........................................................... 217
14.1 Introduction ........................................................ 217
14.2 Protocol ............................................................ 220
14.2.1 Materials ................................................... 220
14.2.2 Instruments ................................................ 220
14.2.3 Reagents ................................................... 220
14.2.4 Procedures ................................................. 221
14.3 Application and Limitation ........................................ 224
References ................................................................ 224
Part VIII Other Methods
15 Splinted Ligation Method .............................................. 229
15.1 Introduction ........................................................ 230
15.2 Protocol ............................................................ 230
15.2.1 Materials ................................................... 230
15.2.2 Instruments ................................................ 230
15.2.3 Reagents ................................................... 232
15.2.4 Procedures ................................................. 233
15.3 Application and Limitation ........................................ 238
References ................................................................ 239
16 Padlock-Probes and Rolling-Circle Amplification ................... 241
16.1 Introduction ........................................................ 241
16.2 Protocol ............................................................ 243
16.3 Materials ........................................................... 243
16.3.1 Instruments ................................................ 243
16.3.2 Reagents ................................................... 243
16.3.3 Procedures ................................................. 244
16.4 Application and Limitation ........................................ 246
16.4.1 Applications ............................................... 246
16.4.2 Limitations ................................................. 247
References ................................................................ 247
17 Invader Assay ........................................................... 249
17.1 Introduction ........................................................ 249
17.2 Protocol ............................................................ 250
17.2.1 Materials ................................................... 250
xvi Contents
17.2.2 Instruments ................................................ 250
17.2.3 Reagents ................................................... 250
17.2.4 Procedures ................................................. 251
17.3 Application and Limitation ........................................ 255
References ................................................................ 255
18 Single Molecule Method ................................................ 257
18.1 Introduction ........................................................ 257
18.2 Protocol ............................................................ 258
18.2.1 Materials ................................................... 258
18.2.2 Instruments ................................................ 259
18.2.3 Reagents ................................................... 259
18.2.4 Procedures ................................................. 259
18.3 Application and Limitation ........................................ 263
References ................................................................ 264
19 Enzymatic Method ...................................................... 267
19.1 Introduction ........................................................ 267
19.2 Protocol ............................................................ 269
19.2.1 Materials ................................................... 269
19.2.2 Instruments ................................................ 269
19.2.3 Reagents ................................................... 269
19.2.4 Procedures ................................................. 270
19.3 Application and Limitation ........................................ 274
References ................................................................ 274
20 Surface-Enhanced Raman Spectroscopy Method .................... 275
20.1 Introduction ........................................................ 275
20.2 Protocol ............................................................ 277
20.2.1 Materials ................................................... 277
20.2.2 Instruments ................................................ 277
20.2.3 Reagents ................................................... 277
20.2.4 Procedures ................................................. 278
20.3 Application and Limitation ........................................ 280
References ................................................................ 280
21 RAKEAssay ............................................................ 281
21.1 Introduction ........................................................ 281
21.2 Protocol ............................................................ 283
21.2.1 Materials ................................................... 283
21.2.2 Instruments ................................................ 283
21.2.3 Reagents .................................................. 283
21.2.4 Procedures ................................................. 284
Contents Xvii
21.3 Application and Limitation ........................................ 287
References ................................................................ 287
22 Bead-Based Flow Cytometric miRNA Expression Profiling ......... 289
22.1 Introduction ........................................................ 289
22.2 Protocol ............................................................ 290
22.2.1 Materials ................................................... 290
22.2.2 Instruments ................................................ 290
22.2.3 Reagents ................................................... 290
22.2.4 Procedures ................................................. 290
22.3 Application and Limitation ........................................ 293
References ................................................................ 294
23 Bioluminescence miRNA Detection Method .......................... 295
23.1 Introduction ........................................................ 295
23.2 Protocol ............................................................ 296
23.2.1 Materials ................................................... 296
23.2.2 Instruments ................................................ 297
23.2.3 Reagents ................................................... 297
23.2.4 Procedures ................................................. 298
23.3 Application and Limitation ........................................ 301
References ................................................................ 302
24 Molecular Beacon Method ............................................. 303
24.1 Introduction ........................................................ 303
24.2 Protocol ............................................................ 306
24.2.1 Materials ................................................... 306
24.2.2 Instruments ................................................ 307
24.2.3 Reagents ................................................... 307
24.2.4 Procedures ................................................. 307
24.3 Application and Limitation ........................................ 310
References ................................................................ 310
25 Ribozyme Method ....................................................... 313
25.1 Introduction ........................................................ 313
25.2 Protocol ............................................................ 315
25.2.1 Materials ................................................... 315
25.2.2 Instruments ................................................ 315
25.2.3 Reagents ................................................... 315
25.2.4 Procedures ................................................. 316
25.3 Application and Limitation ........................................ 318
References ................................................................ 319
xviii Contents
26 Electrocatalytic Moiety Labeling Technique for
High-Sensitivity miRNA Expression Analysis ........................ 321
26.1 Introduction ........................................................ 321
26.2 Protocol ............................................................ 322
26.2.1 Materials ................................................... 322
26.2.2 Instruments ................................................ 323
26.2.3 Reagents ................................................... 324
26.2.4 Procedures ................................................. 324
26.3 Application and Limitation ........................................ 327
References ................................................................ 328
Part IX Circulating miRNA Detection Methods
27 Serum and Plasma miRNA Detection ................................. 331
27.1 Introduction ........................................................ 331
27.2 Protocol ............................................................ 332
27.2.1 Materials ................................................... 332
27.2.2 Instruments ................................................ 333
27.2.3 Reagents ................................................... 333
27.2.4 Procedures ................................................. 333
27.3 Application and Limitation ........................................ 337
References ................................................................ 337
28 miRNA Detection from Peripheral Blood Microvesicles ............ 339
28.1 Introduction ........................................................ 339
28.2 Protocol ............................................................ 340
28.2.1 Materials ................................................... 340
28.2.2 Instruments ................................................ 340
28.2.3 Reagents ................................................... 341
28.2.4 Procedures ................................................. 341
28.3 Application and Limitation ........................................ 343
References ................................................................ 343
29 Detection of Placental miRNAs in Maternal Plasma ................. 345
29.1 Introduction ........................................................ 345
29.2 Protocol ............................................................ 346
29.2.1 Materials ................................................... 346
29.2.2 Instruments ................................................ 346
29.2.3 Reagents ................................................... 346
29.2.4 Procedures ................................................. 347
29.3 Application and Limitation ........................................ 348
References ................................................................ 349
Contents xix
Part X Single-cell miRNA Detection Methods
30 Quantitative LNA-ELF-FISH Method for miRNA
Detection in Single Mammalian Cell .................................. 353
30.1 Introduction ........................................................ 353
30.2 Protocol ............................................................ 354
30.2.1 Materials ................................................... 354
30.2.2 Instruments ................................................ 354
30.2.3 Reagents ................................................... 355
30.2.4 Procedures ................................................. 355
30.3 Application and Limitation ........................................ 358
References ................................................................ 358
31 Single Cell Stem-Looped Real-Time PCR ............................ 361
31.1 Introduction ........................................................ 361
31.2 Protocol ............................................................ 363
31.2.1 Materials ................................................... 363
31.2.2 Instruments ................................................ 363
31.2.3 Reagents ................................................... 364
31.2.4 Procedures ................................................. 364
31.3 Application and Limitation ........................................ 367
References ................................................................ 367
32 miRNA Function-Reporter Expression Assay ........................ 369
32.1 Introduction ........................................................ 369
32.2 Protocol ............................................................ 370
32.2.1 Materials ................................................... 370
32.2.2 Instruments ................................................ 371
32.2.3 Reagents ................................................... 371
32.2.4 Procedures ................................................. 371
32.3 Application and Limitation ........................................ 373
References ................................................................ 374
Part XI Whole Mount In Situ Analysis
33 Whole Mount In Situ Hybridization (WM-ISH) for miRNA
Expression Profiling During Vertebrate Development .............. 377
33.1 Introduction ........................................................ 377
33.2 Protocol ............................................................ 379
33.2.1 Materials ................................................... 379
33.2.2 Instruments ................................................ 379
xx Contents
33.2.3 Reagents ................................................... 379
33.2.4 Procedures ................................................. 380
33.3 Application and Limitation ........................................ 383
References ................................................................ 384
Index .......................................................................... 385
|
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| id | DE-604.BV036107924 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:11:47Z |
| institution | BVB |
| isbn | 9783642049279 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018998153 |
| oclc_num | 449852660 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-11 |
| physical | XX, 387 S. Ill., graph. Darst. |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | Springer |
| record_format | marc |
| spelling | Wang, Zhiguo Verfasser aut MicroRNA expression detection methods Zhiguo Wang ; Baofeng Yang Berlin [u.a.] Springer 2010 XX, 387 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Gene Expression Profiling methods Gene expression MicroRNAs RNA Genexpression (DE-588)4020136-3 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Small RNA (DE-588)7501213-3 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Transkription Genetik (DE-588)4185906-6 gnd rswk-swf Small RNA (DE-588)7501213-3 s Transkription Genetik (DE-588)4185906-6 s Biochemische Analyse (DE-588)4255721-5 s DE-604 Genexpression (DE-588)4020136-3 s Methode (DE-588)4038971-6 s b DE-604 Yang, Baofeng Verfasser aut HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018998153&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Wang, Zhiguo Yang, Baofeng MicroRNA expression detection methods Gene Expression Profiling methods Gene expression MicroRNAs RNA Genexpression (DE-588)4020136-3 gnd Methode (DE-588)4038971-6 gnd Small RNA (DE-588)7501213-3 gnd Biochemische Analyse (DE-588)4255721-5 gnd Transkription Genetik (DE-588)4185906-6 gnd |
| subject_GND | (DE-588)4020136-3 (DE-588)4038971-6 (DE-588)7501213-3 (DE-588)4255721-5 (DE-588)4185906-6 |
| title | MicroRNA expression detection methods |
| title_auth | MicroRNA expression detection methods |
| title_exact_search | MicroRNA expression detection methods |
| title_full | MicroRNA expression detection methods Zhiguo Wang ; Baofeng Yang |
| title_fullStr | MicroRNA expression detection methods Zhiguo Wang ; Baofeng Yang |
| title_full_unstemmed | MicroRNA expression detection methods Zhiguo Wang ; Baofeng Yang |
| title_short | MicroRNA expression detection methods |
| title_sort | microrna expression detection methods |
| topic | Gene Expression Profiling methods Gene expression MicroRNAs RNA Genexpression (DE-588)4020136-3 gnd Methode (DE-588)4038971-6 gnd Small RNA (DE-588)7501213-3 gnd Biochemische Analyse (DE-588)4255721-5 gnd Transkription Genetik (DE-588)4185906-6 gnd |
| topic_facet | Gene Expression Profiling methods Gene expression MicroRNAs RNA Genexpression Methode Small RNA Biochemische Analyse Transkription Genetik |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018998153&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT wangzhiguo micrornaexpressiondetectionmethods AT yangbaofeng micrornaexpressiondetectionmethods |